• Journal of Korea Water Resources Association
• /
• v.38 no.9 s.158
• /
• pp.699-711
• /
• 2005

In this study, the U.S. Geological Survey's DR3M-II(Distributed Routing Rainfall-Runoff Model) was applied for small urban drainage. DR3M-II is a watershed model for routing storm runoff through a branched system of pipes and natural channels using rainfall input. The model was calibrated and verified using short term rainfall-runoff data collected from Sanbon basin. Also, the parameters were optimized using Rosenbrock technic. An estimated simulation error for peak discharge was about 7.4 percent and the result was quite acceptable. Results of the sensitivity analysis indicate that the percent of effective impervious area and ${\alpha}$ defining surface slope and roughness were the most sensitive variables affecting runoff volumes and peak discharge for low and high intensity storm respectively. In most cases, soil moisture accounting and infiltration parameters are the variables that give more effects to runoff volumes than peak discharge. Parameter ${\alpha}$ showed the opposite result.

• Journal of the Korean Chemical Society
• /
• v.54 no.5
• /
• pp.515-522
• /
• 2010

A relation between antimicrobial activities and the formation constants of solid complexes of Cu(II), Ni(II), Co(II), Mn(II) and Fe(III) with tridentate Schiff base ligand, 4-hydroxy-3(1-{2-(benzylideneamino)-phenylimino}-ethyl)-6-methyl-2Hpyran-2-one (HL) derived from o-phenylene diamines, dehydroacetic acid (DHA) and p-chloro benzaldehyde have been studied. The ligand and metal complexes were characterized by elemental analysis, conductivity, magnetic susceptibility, thermal analysis, X-ray diffraction, IR, $^1H$-NMR, UV-vis and mass spectra. From the analytical data, the stiochiometry of the complexes was found to be 1:2 (metal:ligand) with octahedral geometry. The molar conductance values suggest the nonelectrolytic nature of metal complexes. The X-ray diffraction data suggests monoclinic crystal system for Ni(II) and orthorhombic crystal system for Cu(II) and Co(II) complexes. The IR spectral data suggest that the ligand behaves as tridentate ligand with ONN donor atoms sequence towards central metal ion. Thermal behavior (TG/DTA) and kinetic parameters calculated by Coats-Redfern method suggests more ordered activated state in complex formation. The protonation constants of the complexes were determined potentiometrically in THF:water (60:40) medium at $25^{\circ}C$ and ionic strength ${\mu}=0.1\;M$ ($NaClO_4$). Antibacterial activities in vitro were performed against Staphylococcus aureu and Escherichia coli. Antifungal activities were studied against Aspergillus Niger and Trichoderma. The effect of the metal ions and stabilities of complexes on antimicrobial activities are discussed.

• Mycobiology
• /
• v.40 no.1
• /
• pp.20-26
• /
• 2012

The complexes of tailor made ligands with life essential metal ions may be an emerging area to answer the problems of multi drug resistance. The coordination complexes of VO(II), Co(II), Ni(II) and Cu(II) with the Schiff bases derived from isatin with 3-chloro-4-floroaniline and 2-pyridinecarboxaldehyde with 4-aminoantipyrine have been synthesized by conventional as well as microwave methods. These compounds have been characterized by elemental analysis, molar conductance, electronic spectra, FT-IR, FAB mass and magnetic susceptibility measurements. FAB mass data show degradation of complexes. Both the ligands behave as bidentate and tridentate coordinating through O and N donor. The complexes exhibit coordination number 4, 5 or 6. The Schiff base and metal complexes show a good activity against the bacteria; $Staphylococcus$ $aureus$, $Escherichia$ $coli$ and $Streptococcus$ $fecalis$ and fungi $Aspergillus$ $niger$, $Trichoderma$ $polysporum$, $Candida$ $albicans$ and $Aspergillus$ $flavus$. The antimicrobial results also indicate that the metal complexes are better antimicrobial agents as compared to the Schiff bases. The minimum inhibitory concentrations of the metal complexes were found in the range 10-40 ${\mu}g/mL$.

• Journal of the Korean Chemical Society
• /
• v.53 no.4
• /
• pp.407-414
• /
• 2009

The solid complexes of Cu(II), Ni(II), Co(II), Mn(II) and Fe(III) with 4-hydroxy-3-(1-{2-(2-hydroxybenzylidene)- amino phenylimino}-ethyl)-6-methy-pyran-2-one (H2L) derived from o-phenylenediamine, 3-acetyl- 6-methyl-(2H) pyran, 2,4 (3H)-dione (dehydroacetic acid or DHA) and salicylic aldehyde have been synthesized and characterized by elemental analysis, conductometry, magnetic susceptibility, UV-visible, IR, $^1H$-NMR spectra, X-ray diffraction, thermal analysis, and screened for antimicrobial activity. The IR spectral data suggest that the ligand behaves as a dibasic tetradentate ligand with ONNO donor atoms sequence towards central metal ion. From the microanalytical data, the stoichiometry of the complexes has been found to be 1:1 (metal: ligand). The physico-chemical data suggests square planar geometry for Cu(II) and Ni(II) complexes and octahedral geometry for Co(II), Mn(II) and Fe(III) complexes. The x-ray differaction data suggests orthorhombic crystal system for Cu(II) complex, monoclinic crystal system for Ni(II), Co(II) and Fe(III) and tetragonal crystal system for Mn(II) complex. Thermal behaviour (TG/DTA) of the complexes was studied and kinetic parameters were determined by Coats-Redfern method. The ligand and their metal complexes were screened for antibacterial activity against Staphylococcus aureus and Escherichia coli and fungicidal activity against Aspergillus Niger and Trichoderma.

• Journal of Bio-Environment Control
• /
• v.25 no.3
• /
• pp.162-167
• /
• 2016

This study was carried out to investigate proper stem cutting position and air temperature to regenerate roots from cuttings of bitter gourd. 'NS454' (NS) and 'Dragon' (DR) cultivars were tested and the cutting position was cut at the 3rd node in the stem (treatment I) and cut at the central part (stem segment) between 3rd leaf and 4th leaf in the stem (treatment II). The air temperature was maintained at 18, 23, 28 and $35^{\circ}C$ in the growth chambers, respectively. The photosynthetic photon flux at the ground of a chamber was maintained in approximately $150-200{\mu}mol\;m^{-2}s^{-1}$ during the 16-hour photoperiod. The relative humidity in the chambers was maintained over 85%. After 10 days of cuttings, regardless of cutting position, DR and NS cultivars showed 100% the survival rate in the $18^{\circ}C$ or $23^{\circ}C$ treatments, however, most of plants $18^{\circ}C$ treatment had not rooting. In the $28^{\circ}C$ treatment, regardless of cutting position, DR and NS cultivars showed 100% of the rooting rate. In the $23^{\circ}C$ treatment, for DR cultivar, the node cutting showed 90% of rooting rate and the stem segment cutting showed 40% rooting rate. For NS cultivar, the node cutting showed 50% of rooting rate and the stem segment cutting showed 40% rooting rate. DR and NS cultivars have high rooting rate, while the rooting rate remarkably decreased in the $35^{\circ}C$ treatment. For DR cultivar, the stem segment cutting showed 5.3 of the highest rooting number and the node cutting showed 2.7 rooting number in the $28^{\circ}C$ treatment. NS cultivar was not significantly different the rooting regardless of cutting position. The results suggested that cutting at the stem segment cutting of bitter gourd is an effective cutting method for increasing survival rate and the air temperature should be maintained at $28^{\circ}C$.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.24 no.6
• /
• pp.942-949
• /
• 2010

Morus alba L., a kind of Oriental medicinal herbs, has been traditionally used to treat pulmonary asthma and congestion. According to recent studies, extracts of M. alba L. have showed anti-inflammatory, anti-oxidant, anti-tumor and hypoglycemic effects. However, the molecular mechanisms on how it acts as a death-inducer in cancer cells have not been fully understood. In this study, we investigated the cell death effects of methylene chloride extracts of M. alba L. (MEMA) in A549 human lung carcinoma cells. It was shown that MEMA induced the apoptotic cell death proved by increased sub-G1 phase cell population, apoptotic body formation and chromatin condensation. MEMA treatment induced the expression of death receptor-related proteins such as death receptor (DR) 4, DR5, Fas and FasL, which further triggered the activation of caspase-8 and the cleavage of Bid in a concentration-dependent manner. However, MEMA reduced anti-apoptotic Bcl-2 and Bcl-xL expression which contributed to the loss of mitochondrial membrane potential (MMP), and the activations of caspase-9 and caspase-3. Meanwhile, the morphological study indicated a characteristic finding of autophagy, such as the formation of autophagosomes in MEMA-treated cells. Furthermore, markers of autophagy, namely, the increased MDC-positive cells, conversion of microtubule-associated protein light chain 3 (LC3)-I to LC3-II and increased beclin-1 accumulation, were observed. Taken together, these findings demonstrated that MEMA triggered both autophagy and apoptosis in A549 cancer cells. They might suggest that M. alba L. could be a prospective clinical application to treat human lung cancers.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.5
• /
• pp.387-394
• /
• 2001

Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

• Journal of the Korean Professional Engineers Association
• /
• v.16 no.3
• /
• pp.5-23
• /
• 1983

On the Seoul Metropolitan Subway Construction of No. 3, 4 Line, the total length is 57 Km and it is now undergoing almost 55％ progress. The working method is classified into Open Cut of 70% and the rest of 30% tunnelling method in the 48 job site. Above tunnelling method is execute by American Steel Support System and the rest of 10 job site carried out by New Austria Tunnelling Method. This paper describes Blasting Standardizations works on the above Tunnelling ' Open Cut Method under big slogan, first safety, second execution. As a superintendent, I strived standardization of works with Better powder, Better Drills ' Better Pattern. Geological structure of Seoul area is composed by Jurassic Granite and also the above rockgroup are over burden by Alluviums as a Unconformity. First of all, I carried out the standard amount of powder and burden through experimental standard blasting by each powder as following Blasting works in the subway construction is surrounding shop Building, under pass the city river and also under pass highest building basement floor. I made allowable Blasting Vibration Value by West-Germany Vornorm DIN 4150, Teil 3 and should measure each blasting works as fellows all of powder is used basically Low-Gravity and Low Velocity such as Slurry, Ammonium Nitrate ' Finex I, II. for Smooth Blasting Instead of Gelatin Dynamite. Electric Detonation Cap is used basically M/S Delay Cup instead of Electric delay ' Simultaneous cap. I applied following formula V=KW3/4 $D^{-2}$ V=Particle Velocity (Cm/sec) K=Ginh Huh's Value W=Delay Charge (Kg) D=Distance(m) In the Open Cut, within 1m distance from H-pile I made to use the Concrete breaker, as following V=7W/$^{0.5}$V/$^{-1.75}$ On the Concentrate Building area, I advise to use Light class drill ø36m Bit and advance 1.1m per round blasting the three boom jumbo drill over ø45mm used only suburb of city.e Light class drill ø36m Bit and advance 1.1m per round blasting the three boom jumbo drill over ø45mm used only suburb of city.

• The Bulletin of The Korean Astronomical Society
• /
• v.43 no.1
• /
• pp.44.1-44.1
• /
• 2018

We present a spectroscopic study of the Galactic supernova remnant (SNR) Cygnus Loop using the fifth Data Release (DR5) of LAMOST. The LAMOST (Large Sky Area Multi-Object Fiber Spectroscopic Telescope) features both a large field-of-view (about 20 deg2) and a large aperture (~4 m in diameter), which allow us to obtain 4000 spectra simultaneously. Its wavelength coverage ranges from ${\sim}3700{\AA}$ to $9000{\AA}$ with a spectral resolution of $R{\approx}1800$. The Cygnus Loop is a prototype of middle-aged SNRs, which has advantages of being bright, large in angular size (${\sim}3.8^{\circ}{\times}3^{\circ}$), and relatively unobscured by dust. Along the line of sight of the Cygnus Loop, 2747 LAMOST DR5 spectra are found in total, which are spatially distributed over the entire remnant. Among them, 778 spectra are selected based on the presence of emission lines (i.e., [O III]${\lambda}5007$, Ha, and [S II]${\lambda}{\lambda}$ 6717, 6731) for further visual inspection. About half of them (336 spectra) show clear spectral features to confirm their association with the remnant, 370 spectra show stellar features only, and 72 spectra are ambiguous and need further investigation. For those associated with the remnant, we identify emission lines and measure their intensities. Spectral properties considerably vary within the remnant, and we compare them with theoretical models to derive physical properties of the SNR such as electron density and temperature, and shock velocity. While some line ratios are in good agreement with model prediction, others cannot be explained by simple shock models with a range of shock velocities. We discuss these discrepancies between model predictions and the observations and finally highlight the powerfulness of the LAMOST data to investigate spatial variations of physical properties of the Cygnus Loop.

• Development and Reproduction
• /
• v.20 no.1
• /
• pp.1-10
• /
• 2016

Molecular targeting for the altered signaling pathways has been proven to be effective for the treatment of many types of human cancer, including colorectal cancer (CRC). The dual phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) inhibitor BEZ235 has shown to exhibit potent antitumor activity against solid tumors. Autophagy is a cellular lysosomal catabolic process to maintain metabolic homeostasis, which has been known to be induced in response to many therapeutic agents in cancer cells. This process is negatively regulated by mTOR and often acts as prosurvival or prodeath mechanism following cancer therapeutics. The current study was designed to investigate the antiproliferation activity of BEZ235 and to evaluate the role of autophagy induced by BEZ235 using HCT15 CRC cells bearing ras oncogene mutation. We found that BEZ235 decreases cell viability, which was mostly dependent on $G_1$ arrest of cell cycle via suppression of cyclin A expression. BEZ235 affects PI3K/Akt/mTOR signaling pathway by increasing the phosphorylation of AKT at $Ser^{473}$ and RAS/RAF/MEK/ERK pathway by decreasing the phosphorylation of ERK at $Tyr^{204}$. BEZ235 also stimulated autophagy induction as evidenced by the increased expression of LC3-II and abundant acidic vesicular organelles (AVOs) in the cytoplasm. In addition, the combination of BEZ235 with autophagy inhibitor chloroquine, a known antagonist of autophagy, counteracted the antiproliferation effect of BEZ235. Thus, our study indicates that autophagy induced in response to BEZ235 treatment appears to act as cell death mechanism in HCT15 CRC cells.