• 한국산림과학회지
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• 제102권4호
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• pp.566-570
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• 2013

정금나무의 기능성 품종을 육성하기 위하여 정금나무 선발 5개체의 열매에 대한 항산화 활성을 측정하였으며, 항산화 성분인 총 페놀 함량을 측정하였다. 총 페놀 함량은 gallic acid를 기준물질로 하여 분석한 결과 15.8~19.9 mg/g 범위로 전체 평균 17.9 mg/g을 나타냈으며, DPPH free radical 소거활성은 400 ppm의 농도에서 69.1~91.4% 범위로 전체평균 84.2%를 나타내었다. 환원력은 0.56~0.96 범위로 전체 평균 0.79를 나타내었다. 상관분석 결과 DPPH free radical 소거활성과 환원력에서 상관계수가 0.969로 매우 높은 정의 상관관계를 나타내었다.

• 산업식품공학
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• 제15권3호
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• pp.221-229
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• 2011

Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix $L_9(3^4)$ method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula were 0.93, indicating 'good correlation'. The optimum extraction conditions was obtained at the extraction time of 1 hr. temperature of $85^{\circ}C$, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were $67.21{\pm}2.17$ and $52.94{\pm}2.10 mg/g$, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

• 농약과학회지
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• 제7권2호
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• pp.92-99
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• 2003

96-well plate를 사용해서 DPPH free radical 소거활성의 고효율검정 (high throughput screening)방법을 확립하였고, 이 방법을 이용하여 107개의 식물특정 효소저해제와 다양한 식물 추출물의 항산화활성을 조사하였다. DPPH free radical 소거활성 측정 측정을 위한 적정 시험조건은 총 반응액이 $250{\mu}L$일 경우 $100{\mu}M$의 DPPH(pH 7.8), 20 분의 반응시간이었고, 이 조건하에서 ascorbate와 a-tocopherol 은 농도 의존적인 항산화활성을 나타내었다. 107개의 식물 특정효소저해제 중에서 11개의 화학물질이 $100{\mu}M$ 농도에서 70% 이상의 항산화활성이 있었는데, 특히 ampicillin과 gallic acid는 각각 90.2% 와 92.6% 의 나타냈다. 또한 100개의 식물 추출물은 $50{\mu}g/mL$ 농도에서 70% 이상의 활성을 보이는 추출물이 11개이었는데, 그 중에서 AT-407의 활성이 90.1%로 가장 높게 나타났다. 따라서 96-well plate를 이용한 DPPH free ra 이때 소거활성 측정방법은 여러가지 합성물질이나 다양한 천연물질에 대하여 보다 간편하고 신속하게 항산화활성을 측정할 수 있을 것으로 사료되었다.

• Archives of Pharmacal Research
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• 제26권7호
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• pp.535-539
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• 2003

The EtOAc soluble fraction from the stem of Sorghum bicolor showed a strong free radical scavenging activity. Five major compounds were isolated from this fraction. They were identified by spectral data as methyl ferulate (1), methyl p-hydroxycinnamate (2), p-hydroxybenzaldehyde (3), tricin (4), and quercetin 3,4 -dimethyl ether (5). Among these compounds, 1 exhibited a strong, free radical scavenging activity on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) with an $IC_50$ value of 0.7 $\mu$M. We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. All five compounds showed anti-lipid peroxidation activity ($IC_50$ values of 0.5, 0.4, 0.3 and 0.3 $\mu$ M, respectively).

• Archives of Pharmacal Research
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• 제25권3호
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• pp.300-305
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• 2002

The EtOAc and n-BuOH soluble fractions from the aerial part of Setaria viridis showed a strong free radical scavenging activity. Six major compounds were isolated from these fractions. They were identified by spectral data as tricin (1), p-hydroxycinnamic acid (2), vitexin 2"-Ο-xyloside (3), orientin 2"-Ο-xyloside (4), $tricin-7-Ο-{\beta}-D-glucoside$ (5) and vitexin 2"-Ο-glucoside (6). Among these compounds, 4 and 5 exhibited strong free radical scavenging activities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH). We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. As expected, 4 and 5 exhibited significant inhibition on $ascorbic/Fe^{2-}$ induced lipid peroxidation in rat liver microsomes.ver microsomes.

• Advances in Traditional Medicine
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• 제4권3호
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• pp.197-209
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• 2004

The study was aimed at evaluating the antioxidant and antimicrobial activities of methanol extract of Caesalpinia bonducella leaves (MECB) (Family: Caesalpiniaceae). The effect of MECB on antioxidant activity, reducing power, free radical scavenging (DPPH radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and hydrogen peroxide radical scavenging), total phenolic content and antimicrobial activities were studied. The antioxidant activity of MECB increased in a dose dependent manner. About 50, 100, 250 and 500 g of MECB showed 53.4, 61.2, 69.1 and 76.2 % inhibition respectively on peroxidation of linoleic acid emulsion. Like antioxidant activity, the effect of MECB on reducing power increased in a dose dependent manner. The free radical scavenging activity of MECB was determined by DPPH radical scavenging method. The potency of this activity was increased with increased amount of extract. MECB was found to inhibit the nitric oxide radicals generated from sodium nitroprusside $(IC_{50}\;=\;102.8\;g/ml)$ whereas the $IC_{50}$ value of curcumin was 20.4 g/ml. Moreover, the MECB was found to scavenge the superoxide generated by photoreduction of Riboflavin. MECB was also found to inhibit the hydroxyl radical generated by Fenton reaction, where the $IC_{50}$ value is 104.17 g/ml compared with catechin 5 g/ml, which indicates the antioxidant activity of MECB. The MECB capable of scavenging hydrogen peroxide in a concentration-dependent manner. The amounts of total phenolic compounds were also determined. Antimicrobial activities of MECB were carried out using disc diffusion methods with five Gram positive, four Gram negative and four fungal species. The results obtained in the present study indicate that MECB leaves are potential source of natural antioxidant and antimicrobial agents.

• Preventive Nutrition and Food Science
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• 제12권3호
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• pp.177-180
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• 2007

Tocotrienols (T3) are minor plant constituents found abundantly in rice bran, which provide a significant source of vitamin E in animal feeds. T3 was reported to have an intrinsic hypocholesterolemic effect by inhibiting HMG-Co A reductase. It has similar antioxidative properties as tocopherols in food and biological system due to their similar chemical structures. However, the antioxidant activity and mechanism of T3 to scavenge free radicals and to inhibit the peroxidation of linoleic acid are less understood. The purpose of this study was to investigate the scavenging effect of T3 on free radicals and its inhibition of peroxide formation. Free radical scavenging activity was monitored by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method whereas inhibition of linoleic acid peroxidation was evaluated using the thiocyanate method. Thiobarbituric acid (TBA) test was used to determine malonaldehyde formation from linoleic acid peroxidation. Free radical scavenging activity increased with increasing concentration levels of T3. T3 exhibited 38.2, 78.6, 92.7 and 96.2% radical scavenging activity at concentrations of 2, 8, 32 and 128 ppm, respectively. At 128 ppm, it was highly effective in inhibiting linoleic acid peroxidation. The activity of T3 evaluated by the thiocyanate method showed low absorbance values indicating a high level of antioxidant activity. All treatments showed similar trends in antioxidant activity when evaluated by both the thiocyanate method and TBA test.

• 한국식품영양과학회지
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• 제32권1호
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• pp.115-118
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• 2003

본 연구는 캠벨종 포도의 종자와 과피 에탄올 추출물의 추출조건과 그에 따른 DPPH법에 의한 free radical 소거 활성을 비교하여 최적의 추출 조건의 확립과 항산화 활성을 살펴보았다. 추출 조건은 추출 용매인 에탄올의 농도와 추출 온도를 달리 하여 각각의 수율을 비교하였으며, 각 분획물은 hexane 분획물, chloroform 분획물, ethyl acetate 분획물, butanol 분획물 및 물 분획물로 나누어, free radical 소거 활성을 RC$_{50}$으로 나타내었다. 추출 수율은 종자에서는 추출 온도 78$^{\circ}C$, 에탄올 농도 100%에서 11.4%로 다른 온도와 농도 조건에 비하여 높은 수율을 나타냈으며, 과피 에서도 역시 추출 온도 78$^{\circ}C$, 에탄올 농도 100%에서 44.5%로 다른 온도와 농도 조건에 비하여 높은 수율을 나타냈다. 각 분획물 중에서는 물 분획물이 종자에서는 39.2%인데 비하여 과피 에서는 85.6%로 다른 분획물에 비하여 많이 차지하였다. DPPH법에 의한free radical 소거 활성에서는 종자의 경우, 5$0^{\circ}C$ 에탄을 추출물이 RC$_{50}$=16.8 $\mu\textrm{g}$/mL로, 분획물에서는 ethyl acetate 분획물이 RC/syb 50/=15.4 $\mu\textrm{g}$/mL로 가장 높은 활성을 나타내었다. 과피의 경우, 78$^{\circ}C$ 에탄올 추출물이 RC$_{50}$=2437.5 $\mu\textrm{g}$/mL로, 분획물에서는 butanol 분획물이 RC$_{50}$=698.4 $\mu\textrm{g}$/mL로 가장 높은 활성을 나타내었다. 이상의 결과를 통하여 추출 온도와 용매 농도에 따른 수율의 차이가 있었으며 free radical 소거 활성에서는 종자 에탄을 추출물이 과피 에탄올 추출물 보다 145배 이상의 현저히 높은 활성을 나타내었다.을 나타내었다.

• 대한본초학회지
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• 제33권6호
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• pp.1-8
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• 2018

Objectives : Herbal medicinal mixture (JMB) are consisted of Caryophylli Flos, Aucklandiae Radix and Angelicae Dahuricae Radix. Each of herbal medicines has studied on anti-oxidant effect. So this study was conducted to investigate efficacy and potency of JMB on anti-oxidation. Methods : The JMB was extracted at room temperature by 80% ethanol. And total polyphenol contents, total flavonoid contents in JMB ethanol extract were determined by colorimetric method. Also, DPPH, ABTS free radical scavenging capacity and reducing power of JMB ethanol extract were measured at 100, 500, 1000, $5000{\mu}g/m{\ell}$ concentrations by spectrometric assay. Positive control was used BHA (butylated hydroxyanisole). Results : The total polyphenol contents and total flavonoid contents of the extract were 55.38 mg/TAEg, 513.72 mg/RUEg, respectively. Also, DPPH free radical scavenging capacity and reducing power of JMB ethanol extract in treated concentrations (100, 500, 1000, $5000{\mu}g/m{\ell}$) increased dose dependently. In particular, DPPH free radical scavenging capacity of JMB ethanol extract at 500, 1000, $5000{\mu}g/m{\ell}$ was similar to positive control (BHA) at high concentration (50, $100{\mu}g/m{\ell}$). ABTS free radical scavenging capacity of JMB ethanol extract at 500, 1000, 5000 ug/ml was similar to BHA at high concentration (50, $100{\mu}g/m{\ell}$). Also, reducing power was showed that JMB ethanol extract at $5000{\mu}g/m{\ell}$ was similar to BHA at high concentration (50, $100{\mu}g/m{\ell}$). Conclusions : These results suggest that JMB ethanol extract has effects to scavenge free radicals. Therefore, JMB has potential and applicable benefits for development of materials and products to have anti-oxidation functions.

• Natural Product Sciences
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• 제13권3호
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• pp.199-206
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• 2007

Antioxidant activity of 70% aqueous ethanolic extract of leaves of Justicia gendarussa (EJ) was evaluated. EJ was prepared by cold maceration method. The antioxidant potency of EJ was investigated employing various established in vitro systems, such as DPPH radical scavenging, nitric oxide (NO) scavenging, ${\beta}-carotene$ linoleic acid module system (${\beta}$ CLAMS), hydroxyl (OH) radical scavenging, anti lipid peroxidation. $IC_{50}$ values were determined in each experiment. Also, ferric ion reduction capacity of extracts in presence and absence of chelating agent (EDTA) and total antioxidant capacity were determined. Preliminary phytochemical investigation was carried out to know the nature of constituents present in the leaves and correlate it with antioxidant activity. Further total phenolic content was determined in EJ. $IC_{50}$ values of EJ were 123.09 ${\pm}$ 3.01, 643.0 ${\pm}$ 61.10, 132.3 ${\pm}$ 6.03, 68.5 ${\pm}$ 11.5 and 68.13 ${\pm}$ 1.38 ${\mu}g/mL$ in DPPH radical scavenging, NO scavenging, ${\beta}$ CLAMS, OH radical scavenging and anti lipid peroxidation activity respectively. In total antioxidant capacity assay, ascorbic acid equivalent value was found to be 205.56 ${\pm}$ 4.69 ${\mu}g/mg$ of extract. Total phenolic content was found to be 43.76 ${\pm}$ 4.27 ${\mu}g$ equivalent of gallic acid per mg of extract. Phytochemical investigation reveals the presence of flavonoids. The results indicate that EJ possess antioxidant activity and flavonoids are responsible for this activity.