• Title/Summary/Keyword: DPPH 소거능

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Quality Characteristics of Omija (Schizandra chinensis) Extracts with Various Water Types (물의 종류에 따른 오미자 추출액의 이화학적 품질특성)

  • Lee, Sung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.706-712
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    • 2013
  • This is the basic study on the extraction characteristics of Omija (Schizandra chinensis) for juices of Korean traditional foods. Extraction is being performed as 25 times of the solvent ratio at room temperature and at $65^{\circ}C$ with deionized water, tap water and Cheong-song mineral water which have high contents of Ca and Mg. The overall good results of the extraction conditions are calculated from the values of L (Lightness), redness (a), yellowness (b), soluble solids content, acidity, pH levels and the DPPH radical scavenging activity by using the electron donating ability (EDA). The results are as follows: 12 hours of room temperature and 6 hours at $65^{\circ}C$ were good extraction times. The Cheong-song mineral water at $65^{\circ}C$ is available for rapid extractions when compared to deionized water and tap water. A longer extraction time shows lower values of acidity but increasing values of soluble solids content and DPPH radical scavenging activity when using the electron donating ability. The best sequences for the extractions are Cheong-song mineral water followed by tap water and distilled water after overall consideration.

9 types of domestic plant combination oil effective for relieving depression Bioactive effect research (우울증완화의 효과적인 국내산 9종 식물 조합오일의 생리활성 효과 연구)

  • Sook-Heui Jung
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.1
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    • pp.103-112
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    • 2023
  • This study selected 9 species of domestic native plants (Baechohyang, Red Pine, Turmeric, Ginger, San parsley seed, Bulsugam, Hwangchil, Tangja, Valerian) that have been proven effective for depression, and based on the analysis results of each fragrance component, 9 species were selected. Antioxidant (DPPH, ABTS), cytotoxicity (MTS), and anti-inflammatory (Nitric oxide) experiments were performed by combining essential oils. As a result of the analysis of fragrance components, DL-Limonene (38.44%), g-Terpinene (8.9%), Estragole (5.18%), and a-Pinene (1.73%), which were previously studied in combination oil, were identified. DPPH radical scavenging ability, which is an antioxidant activity, showed 75.4%, ABTS radical scavenging ability was 74.04%, and NO production inhibition was 33.14% at a concentration of 5uL/ml with no cytotoxicity confirmed. Through this, the effect of domestic blending essential oils on the improvement or prevention of depression is verified, and scientific efficacy and ingredient studies are conducted in a mutually cooperative manner to seek solutions to depression and provide basic data to confirm whether or not depressive symptoms are improved. want to do.

Anti-diabetic and Anti-oxidative Activities of Extracts from Crataegus pinnatifida (산사 추출물의 항산화 및 항당뇨 활성)

  • Nam, Sang-Myeoung;Kang, Il-Jun;Shin, Mee-Hye
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.2
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    • pp.270-277
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    • 2015
  • This study was performed to investigate the antidiabetic and antioxidant activities of Crataegus pinnatifida which was extracted with water and different concentrations of EtOH (0~100%). The extraction yield of 70% EtOH (33.16%) was higher than that of 50% EtOH (27.79%), water (21.71%), 30% EtOH (21.88%) and 100% EtOH (19.03%). Total polyphenol contents of 50% EtOH extract from C. pinnatifida were the highest. DPPH and ABTS radical scavenging activities were $80.79{\pm}0.83%$ and $34.92{\pm}0.97%$ in 50% EtOH extract, respectively, which were higher than those of other extracts. The inhibitory activities of 50% ethanol extract from C. pinnatifida against advanced glycation end products (AGEs) formation and ${\alpha}$-glucosidase were determined to be $27.09{\pm}2.27%$ and $58.87{\pm}0.70%$, respectively. The inhibitory activity of water extract from C. pinnatifida against aldose reductase was higher ($30.68{\pm}1.41%$) than those of other extracts. Overall, 50% EtOH extract from C. pinnatifida showed the highest antidiabetic and antioxidant effects. These results suggest that 50% ethanol extracts from C. pinnatifida have potential as a useful ingredient with antidiabetic and antioxidant effects.

Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Ark shell (Scapharca broughtonii) (피조개의 항산화 활성과 Acetylcholinesterase 저해 활성)

  • Kim, Jeong-Been;Kim, Jae-Min;Lee, Young-Min;Baek, In-Seok;Lee, Seung-Cheol
    • Korean journal of food and cookery science
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    • v.30 no.2
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    • pp.212-218
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    • 2014
  • Ark shell (Scapharca broughtonii; Korean name, pijogae) is one of the most widely cultivated and consumed shellfishes in Korea. The purpose of this study was to evaluate the antioxidant activity and acetylcholinesterase inhibitory activity of ark shell. After preparing the methanol extract of ark shell powder, the extract was subsequently fractionated by hexane, diethyl ether, ethyl acetate and water. The antioxidant activity evaluated by DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power was relatively higher in the water fraction; however, the activity was spread out in all fractions. Acetylcholinesterase inhibitory activity was the highest in the diethyl ether fraction. Taken together with the results of both antioxidant and acetylcholinesterase inhibitory activities, it can be suggested that different kinds of physiological compounds were contained in the ark shell.

Effects of Hot Water Extracts of Domestic Blue Mussel and New Zealand Green Lipped Mussel on Alcohol Metabolizing Enzymatic, DPPH Radical Scavenging, and Angiotensin Converting Enzyme Inhibitory Activities (국산 홍합과 뉴질랜드 초록입 홍합 열수 추출물의 알코올분해효소 활성에 미치는 영향 및 DPPH 라디칼 소거능과 Angiotensin Converting Enzyme 저해 활성)

  • Kim, Si-Kyung;Ok, Dul-Lee;Park, Eunju;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.9
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    • pp.1363-1368
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    • 2014
  • The physiological activities of cultivated Korean blue mussel (Mytilus edulis) and New Zealand green-lipped mussel (Perna canaliculus) were analyzed and compared. Both hot water extracts of blue mussel flesh (BMF) and green-lipped mussel flesh (GMF) showed increased activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). BMF showed increased ADH and slightly decreased ALDH activities compared to GMF. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of BMF was higher than that of GMF at the same concentration. BMF and GMF showed similar inhibitory activity against angiotensin converting enzyme at a concentration of 30 mg/mL. These results suggest that cultivated Korean blue mussel has similar physiological activity with New Zealand green-lipped mussel.

Antioxidant and Xanthine Oxidase Inhibitory Activities of Hot Water Extracts of Medicinal Herbs (한약재 열수추출물의 항산화 활성 및 Xanthine Oxidase 저해 활성)

  • Shin, Yu-Jin;Hwang, Jeong-Man;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.10
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    • pp.1712-1716
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    • 2013
  • In our study, as many as 29 edible medicinal herbs were selected for testing their ability in the effective treatment of gout based on oriental medicine theory. We extracted each medicinal herb (135 g) with 4 L of distilled water at $100{\sim}105^{\circ}C$ for 210 min. Thereafter, we evaluated both the antioxidant and xanthine oxidase inhibition activities of the extracts obtained. Among all the edible medicinal herbs used in our study, only the extract from Scutellaria baicalensis Georgi (Korean name: hwang-geum) showed (1) the maximum total phenolic content (TPC) (2.25 mg gallic acid equivalent/mL), (2) DPPH radical scavenging activity (94.04%), and (3) xanthine oxidase inhibition activity (87.75%). We also observed that TPC was relatively highly correlated with both the DPPH radical scavenging activity (r=0.63) and xanthine oxidase inhibition activity (r=0.77). Our results suggest that S. baicalensis G. may be a potent antioxidant source for the extraction and development of nutraceuticals that may be utilized for effective treatment of gout.

Screening for DPPH Free Radical Scavenging Activities of Autogenous Seaweeds in Jeju Island Using a Electron Spin Resonance (ESR) Spectroscopy (Electron Spin Resonance을 이용한 제주 자생 해조류의 DPPH Free Radical 소거활성 검색)

  • Cha, Seon-Heui;Heo, Soo-Jin;Jeon, You-Jin
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.4
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    • pp.252-259
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    • 2006
  • Extracts which were prepared by four different extractions - 80% methanol extracts (ME) at high ($70^{\circ}C$) and a room temperature ($20^{\circ}C$), respectively and aqueous extracts (AE) at both temperatures with the residue after the methanol extracts - of 10 green, 19 brown and 25 red seaweeds collected in Jeju Island coast were examined for their DPPH free radical scavenging activity using a ESR (electron spin resonance) spectroscopy. A variety of the extracts showed positive scavenging effect against DPPH free radical (except the green seaweeds). Among the extracts, the brown seaweed extracts exhibited the highest scavenging activity. Especially, Sargassum spp. of the brown seaweeds have remarkable scavenging activities - both methanolic and aqueous at the both temperatures ($20^{\circ}C$ and $70^{\circ}C$). On the other hand, ME showed better scavenging activity than AE in the red seaweed extracts. These results indicate that autogenous seaweeds in Jeju will be potential natural antioxidants for functional food compounds.

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Antioxidant activity of hot-water extracts and floral waters from natural plant pigments (천연색소 함유 식물추출물과 플로럴 워터의 항산화 활성)

  • Kim, Yeon-Soon;Suh, Hwa-Jin;Park, Shin
    • Food Science and Preservation
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    • v.22 no.1
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    • pp.129-133
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    • 2015
  • The antioxidant potentials of ten kinds of medicinal plants used as natural dyeing agents were tested. Among the plant extracts and floral waters that were examined in this study, the antioxidant activities of the DPPH and ABTS radical scavengers increased with increasing amounts of the extracts. The hot-water extracts from Aphis chinensis, Caesalpinia sappan L., Rumex crispus L., and Areca catechu effectively inhibited the DPPH and ABTS radicals at concentration below 0.1 mg/mL. The floral water obtained through steam distillation from Areca catechu, Rubia akane Nakai, Coptis chinensis, and Phellodendron amurense showed relatively valid antioxidant activities. In particular, the natural colorants extracted from Rumex crispus L., Areca catechu, and Aphis chinensis effectively suppressed the photogenerated singlet oxygen induced by a photosensitizer in in-vitro assay systems. The concentrations ($IC_{50}$) of the hot-water Aphis chinensis extract required to exert a 50% reduction effect on DPPH, ABTS, and singlet oxygen were found to be 8.5, 8.0, and $210{\mu}g/mL$, respectively. The study results suggest that extracts derived from naturally occurring colorants as well as floral waters could be used as natural antioxidants in the food and health-care industries.

The Change in Biological Activities of Brown Rice and Germinated Brown Rice (품종별 현미 발아 전후의 생리활성물질 변화)

  • Kim, Dae-Jung;Oh, Sea-Kwan;Yoon, Mi-Ra;Chun, A-Reum;Choi, Im-Soo;Lee, Dong-Hyun;Lee, Jun-Soo;Yu, Kwang-Won;Kim, Yeon-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.6
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    • pp.781-789
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    • 2011
  • We studied the biological activities, including antioxidant compounds, antioxidant activities, anti-proliferative activities, and immunological activities of brown rice and germinated brown rice. We examined the DPPH, ABTS radical scavenging activity, and reducing power of 70% ethanol extracts from some cultivars of brown rice and germinated brown rice. The total polyphenol, total flavonoid, and ${\gamma}$-oryzanol contents of the extracts were measured with spectrophotometric methods. The Hongjinjubeyo brown rice and germinated brown rice extracts showed markedly higher antioxidative activity than those of 70% ethanol extracts from other cultivars. The 70% ethanol extracts from brown rice and germinated brown rice had the most effective anti-proliferative activity (cytotoxicity) against breast cancer cells (MCF-7) compared to colorectal cancer cells (HCT-116). A $500\;{\mu}g$/mL concentration of 70% Hongjinjubyeo ethanol extract had higher macrophage and mitogenic activities of immunological activity than other cultivars.

Food Quality of Muffin with Germinated Brown Rice Soaked in Mycelial Culture Broth of Phellinus linteus (상황버섯균사체 배양액 침지 발아현미를 첨가한 머핀의 식품학적 특성)

  • Jung, Kyong-Im;Cho, Eun-Kyung;Choi, Young-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.6
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    • pp.875-884
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    • 2011
  • The purpose of this study was to examine the qualities of optimized muffins with germinated brown rice soaked in mycelial culture broth of Phellinuslinteus (GBRP) using response surface methodology. Firstly, general compositions of optimized muffins with GBRP were higher than that of control and total sugar contents were similar. However, the total free amino acid and constitutional amino acid contents except for GABA were lower than those of control. Starch hydrolysis in control was higher than in optimized muffins with GBRP, whereas protein digestibility and protein efficiency ratio were not. The weights of optimized muffins with GBRP were higher than that of control (p<0.01), whereas height (p<0.01) and pH (p<0.001) were similar. The hardness (p<0.05) and chewiness (p<0.05) of optimized muffins with GBRP were higher compared to control; adhesiveness, springiness, and gumminess were similar, but cohesiveness (p<0.01) was not. The flavor (p<0.05) and taste (p<0.01) of optimized muffins with GBRP were higher than those of control; appearance, texture and overall acceptability were similar, but color (p<0.05) was not. The total polyphenol contents (p<0.01), DPPH radical scavenging activity (p<0.01), and superoxide dismutase-like activity (p<0.05) of optimized muffins with GBRP were higher than those of control, but nitrite scavenging activity was similar.