DOI QR코드

DOI QR Code

Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Ark shell (Scapharca broughtonii)

피조개의 항산화 활성과 Acetylcholinesterase 저해 활성

  • Kim, Jeong-Been (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Jae-Min (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Young-Min (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Baek, In-Seok (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
  • 김정빈 (경남대학교 식품생명학과) ;
  • 김재민 (경남대학교 식품생명학과) ;
  • 이영민 (경남대학교 식품생명학과) ;
  • 백인석 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Received : 2014.02.03
  • Accepted : 2014.04.09
  • Published : 2014.04.30

Abstract

Ark shell (Scapharca broughtonii; Korean name, pijogae) is one of the most widely cultivated and consumed shellfishes in Korea. The purpose of this study was to evaluate the antioxidant activity and acetylcholinesterase inhibitory activity of ark shell. After preparing the methanol extract of ark shell powder, the extract was subsequently fractionated by hexane, diethyl ether, ethyl acetate and water. The antioxidant activity evaluated by DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power was relatively higher in the water fraction; however, the activity was spread out in all fractions. Acetylcholinesterase inhibitory activity was the highest in the diethyl ether fraction. Taken together with the results of both antioxidant and acetylcholinesterase inhibitory activities, it can be suggested that different kinds of physiological compounds were contained in the ark shell.

국내 연안에서 널리 양식되고 있는 피조개의 생리적 기능성을 발굴하기 위하여 항산화능과 acetylcholinesterase 저해능을 측정하였다. 피조개 분말로부터 메탄올 추출물을 제조하고, 이로부터 극성에 따라 hexane, diethyl ether, ethyl acetate, 그리고 물 분획물을 얻었다. DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력으로 항산화능을 분석한 결과, 각 활성은 한 분획물에 치중되지 않았지만 물 분획물에서 비교적 높았다. 그러나 acetylcholinesterase 저해능은 diethyl ether 분획물에서 높게 측정되었다. 이로서 항산화능과 acetylcholinesterase 저해능에 관여하는 다양한 물질들이 피조개에 함유되어 있음을 확인하였다.

Keywords

References

  1. An MI, An KW, Choi CY. 2009. Changes in antioxidant enzyme activity and physiological responses to cadmium and tributyltin exposure in the ark shell, Scapharca broughtonii. Mol Cell Toxicol 5(4):273-282
  2. An MI, Choi CY. 2010. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters. Comp Biochem Phys B 155(1):34-42
  3. Diplock AT. 1997. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad 27(5):511-532
  4. Fisheries Information Service. www.fips.go.kr. Accessed February 3, 2014
  5. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. 2011. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal 24(7):1043-1048 https://doi.org/10.1016/j.jfca.2011.01.008
  6. Food Composition Table. Rural Resources Development Institute. 2006
  7. Ijaz A, Itrat A, Abdul M, Sarfraz AN, Muhammad IC. 2003. Cholinesterase inhibitory constituents from Onosma hispida. Chem Pharm Bull 51(4):412-414 https://doi.org/10.1248/cpb.51.412
  8. Jang CH, Eun JS, Park HW, Seo SM, Yang JH, Leem KH, Oh SH. Oh CH, Baek NI, Kim DK. 2003. An acetylcholinesterase inhibitor from the leaves of Securinega suffruticosa. Korean J Pharmacogn 34(1):14-17
  9. Jeong SM, Kim SY, Park HR, Lee SC. 2004. Effect of far-infrared radiation on the activity of extracts from Citrus unshiu peels. J Korean Soc Food Sci Nutr 33(9):1580-1583 https://doi.org/10.3746/jkfn.2004.33.9.1580
  10. Kim HL, Kang SG, Kim IC, Kim SJ, Kim DW, Ma SJ, Gao T, Li H, Kim MJ, Lee TH, Ham KS. 2006. In vitro anti-hypertensive, antioxidant and anticoagulant activities of extracts from Haliotis hannai. J Korean Soc Food Sci Nutr 35(7):835-840 https://doi.org/10.3746/jkfn.2006.35.7.835
  11. Kim HY. 1988. Studies on the utilization of arkshell 1. Preparation and quality stability during storage of powdered dried ark shell for instant soup. Korean J Food Hygiene 3(4):217-223
  12. Lahiri DK, Farlow MR, Greig NH, Sambamurti K. 2002. Current drug targets for Alzheimer's disease treatment. Drug Devel Res 56(3):267-281 https://doi.org/10.1002/ddr.10081
  13. Lee DW, You DH, Yang EK, Jang IC, Bae MS, Jeon YJ, Kim SJ, Lee SC. 2010. Antioxidant and ACE inhibitory activities of Styela clava according to harvesting time. J Korean Soc Food Sci Nutr 39(3):331-336 https://doi.org/10.3746/jkfn.2010.39.3.331
  14. McGleenon BM, Dynan KB, Passmore AP. 1999. Acetylcholinesterase inhibitors in Alzheimer's disease. Br J Clin Pharmacol 48(4):471-480
  15. National Fisheries Research Development Institute. Nutrient Database for Ark shell Available from: http://portal.nfrdi.re.kr/ page?id=aq_seafood_2_7&type=tot&from=totList&fim_col_i d=2009-MF0004224-6-D01. Accessed February 3, 2014
  16. Nose Y, Hanyu I, Iwai T, Shimizu M. 1989. Encyclopedia of Fishes. Tokyodoo Syuksubanbu, Tokyo, Japan. p 9
  17. Oyaizu M. 1996. Studies on product of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jap J Nurt 44(6):307-315
  18. Park CK. 2002. Comparison of extractive nitrogenous constituents in the three species of raw bloody clams, Scaphrca broughtonii, S. subcrenata, and Tegellarca granosa extracts. Korean J Food Sci Technol 34(6):954-961
  19. Pellegrini N, Re R, Yang M, Rice-Evans C. 1999. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline- 6-sulfonic acid) radical cation decolorization assay. Vol 299. pp 379-389. In: Methods in Enzymololy. Packer L (ed). Academic Press. New York, NY. USA
  20. Prashanth D, Vinutha B, Salma KM, Sreeja SL, Pratiti D, Padmaja R, Radhika S, Amit A, Venkateshwarlu K, Deepak M. 2007. Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. J Ethnopharmacol 109(2):359-363 https://doi.org/10.1016/j.jep.2006.06.014
  21. Pulok KM, Venkatesan K, Mainak M, Peter JH. 2007. Acetylcholinesterase inhibitors from plants. Phytomedicine 14(4):289-300 https://doi.org/10.1016/j.phymed.2007.02.002
  22. Richard D, Kefi K, Barbe U, Bausero P, Visioli F. 2008. Polyunsaturated fatty acids as antioxidants. Pharmacol Res 57(6):451–455 https://doi.org/10.1016/j.phrs.2008.05.002
  23. Rollinger JM, Hornick A, Langer T, Stuppner H, Prast H. 2004. Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products. J Med Chem 47(25):6248-6254 https://doi.org/10.1021/jm049655r
  24. Shimizu M, Yamaguchi K, Takiguchi M. 1989. Fish Guide Book. Jookoeiyodaikaku Syuksubanbu, Tokyo, Japan. p 102
  25. Song JE, Lee JS. 2008. Physicochemical characteristics of antidementia acetylcholinesterase inhibitor-containing methanol extract from Sorghum bicolor and industrial application. J Natural Sci Pai Chai Univ 19(1):45-55
  26. Tang XC, Han YF. 1999. Pharmacological profile of huperzine A, a novel acetylcholinesterase inhibitor from chinese herb. CNS Drug Reviews 5(3):281-300
  27. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. 2002. Free radical scavenging properties of wheat extracts. J Agric Food Chem 50(6):1619-1624 https://doi.org/10.1021/jf010964p

Cited by

  1. Sensory characteristics of Step-by-Step Sodium Reduction on Frequently used High Sodium Foods in the Institutional Food Service Industry vol.31, pp.4, 2015, https://doi.org/10.9724/kfcs.2015.31.4.465
  2. 식물성 압착오일의 흡착제에 따른 항산화 활성 비교 vol.19, pp.4, 2018, https://doi.org/10.5762/kais.2018.19.4.57
  3. 반응표면분석법을 활용한 피조개(Scapharca broughtonii) 조미가공품용 조미 소스의 공정 최적화 vol.53, pp.3, 2014, https://doi.org/10.5657/kfas.2020.0334
  4. Ameliorative effects of ark clams (Scapharca subcrenata and Tegillarca granosa) on endothelial dysfunction induced by a high-fat diet vol.63, pp.1, 2014, https://doi.org/10.1186/s13765-020-00556-4