• Journal of Life Science
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• v.29 no.4
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• pp.428-435
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• 2019

Alaska pollock (Gadus chalcogrammus) is a marine fish species belonging to the family Gadidae. In this study, whether the Alaska Polloack Roe have antioxidant properties, 2,2-diphenyl-1 picrylhydroazyl (DPPH), hydroxyl radical (OH) reducing activity, and nitric oxide radical (NO) scavenging activity were evaluated in distilled water extract (DWE) and ethanol extract (ETE) of raw Alaska pollock roe, Gochujang Pollock roe, and fermented seasoned Pollock roe. The DPPH scavenging activity of the DWE with Gochujang Pollock roe was 71.9% at a concentration of 1.0 mg/ml and that of the ETE was 73.7% at the same concentration. The DPPH scavenging activity of the DWE with fermented seasoned Pollock roe was 78.0% at 1.0 mg/ml, whereas that of ETE was 78.4% at the same concentration. The $IC_{50}$ values of the DWE and ETE of raw Pollock roe for DPPH were $11.65{\mu}g/ml$ and $11.47{\mu}g/ml$, respectively. The OH scavenging activities of raw Pollock roe, Gochujang Pollock roe, and fermented seasoned Pollock roe ethanolic extracts at a concentration of 1.0 mg/ml were 70.9%, 79.0, and 80.6%, respectively. The $IC_{50}$ values of the DWE and EWE of raw Pollock roe for NO were $11.45{\mu}g/ml$ and $11.41{\mu}g/ml$, respectively. The DPPH, OH, and NO scavenging abilities in DWEs and ETEs of Gochujang and fermented seasoned Pollock roe were higher than those of instant (no Gochujang or season treatment) treatment Pollock roe. Both the Gochujang and fermented seasoned Pollock roes have natural radical scavenging ability and may be useful potential antioxidant food supplements.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.12
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• pp.1793-1798
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• 2015

Antioxidant activities of various solvent extracts from Coriolus versicolor were investigated for their total polyphenol content, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity. C. versicolor extracts were produced by organic solvents such as ethanol, ethyl acetate, and methanol. Total polyphenol and flavonoid contents in the ethanol and ethyl acetate extracts were higher than those in the methanol extract. DPPH radical scavenging activity of methanol extract showed the highest value of 80.3%, which was similar to that of ascorbic acid (85.5%). All extracts showed good (>90.0%) ABTS cation radical scavenging activity. The antimicrobial activities of these extracts from C. versicolor were evaluated against six strains of bacteria using the disc diffusion method. All extracts showed antimicrobial activities against all tested bacteria except Staphylococcus aureus. These results indicate that various extracts from C. versicolor could be useful as natural antioxidant and antimicrobial agents.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.111.1-111
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• 2003

삼백초 지상부의 에탄올 추출농도와 추출 시간에 따른 수율, total phenol과 total flavonoid함량, DPPH 라디칼 소거능을 조사하였다. 추출 온도 9$0^{\circ}C$에서 에탄올농도를 증가시키면서 8시간동안 환류추출하였다. 추출수율과 DPPH 라디칼 소거능은 에탄올농도 40%까지는 증가하다가 60%이상에서는 감소하였고 total phenolic compound와 total flavonoid의 함량도 비슷한 경향을 보였다. total phenolic compound의 함량은 40%와 60%에서 큰 차이가 없는 반면 total flavonoid의 함량은 40%에서 더 높았다. total phenolic compound와 total flavonoid 함량, DPPH 라디칼 소거능 및 추출수율은 40% 이상에서는 큰 변화가 없어 추출용매의 조건은 40% 에탄올로 선정하였다. 40% 에탄올로 9$0^{\circ}C$에서 24시간 동안 4시간 간격으로 환류추출하였을 때 total phenolic compound와 total flavonoid 함량, DPPH 라디칼 소거능 및 추출수율은 4시간 이후 큰 차이가 없었다. 삼백초의 용매추출조건으로는 40% 에탄올, 4시간 추출하는 것이 적합하다고 판정된다.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.426-432
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• 2013

The results of the content of total polyphenol and flavonoid, DPPH (1-1-diphenyl-2-picryl-hydrazyl) radical scavenging activity, ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging activity, nitrite scavenging activity and SOD-like activity of methanol extracts from immature cotton boll are follows. The contents of total polyphenol and flavonoid compound were higher in small size cotton boll, and DPPH and ABTS radical scavenging activity also showed a relatively high activity in the small size. These results indicate that there is a correlation between phenol content and DPPH radical scavenging, ABTS radical scavenging. The test concentrations of immature cotton boll extract for measuring DPPH and ABTS radical scavenging activities were set as 1.25, 2.5, 5, 10 and 20 mg/ml. Immature cotton boll has high radical scavenging activity at the concentration of 1.25~20 mg/ml and the result showed a tendency to increase in a concentration-dependent. The nitrite scavenging activity showed high activity in the pH 1.2, and the result in the pH 4.2 showed progressively less active, and in the pH 6.0 near neutral was confirmed that does not affect the nitrite scavenging. In addition, SOD-like activity showed somewhat lower activity compared with ascorbic acid, but tended to be higher when compared with the results of the other natural substances. Through this experiment, we confirmed that immature cotton boll was excellent antioxidant activity. Therefore, it is demonstrated that the cotton suggest the possibility of development of new material for cosmetic product or functional food in the future, and is expected to make a greater usability.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.631-642
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• 2017

Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to hydrolysis of Ogae egg white protein using the six proteases. The antioxidant activity of the produced peptides was analyzed. As a result, the maximum value of hydrolysis was protamex(46.3%), DPPH radical scavenging was bromelain(57.23%), hydroxy radical scavenging was alcalase(30.21%), superoxide radical scavenging was alcalase(58.07%), and $Fe^{2+}$ chelation ability was alcalase(72.06%). Furthermore, the antioxidant Inhibition concentration ($IC_{50}$) of peptides was evaluated for each enzyme. As a result, the maximum value of alcalase was $Fe^{2+}$ cheating ability($IC_{50}$, 1.24 mg/mL), bromelain was DPPH radical scavenging($IC_{50}$, 2.46 mg/mL), flavourzyme was $Fe^{2+}$ cheating ability($IC_{50}$, 1.25 mg/mL), neutrase was DPPH radical scavenging($IC_{50}$, 3.64 mg/mL), papain was DPPH radical scavenging ($IC_{50}$, 3.82 mg/mL) and protamex was DPPH radical scavenging($IC_{50}$, 1.93 mg/mL). Therefore, we expect that peptides produced from Ogae egg white protein using protease enzyme are useful as an antioxidant functional food ingredients.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.1893-1898
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• 2013

Seaweeds, laver, sea mustard, kelp, and fusiformis, were prepared and investigated for its antioxidant and tyrosinase inhibition activities. The extracts yield, color, total phenolic contents, antioxidative activity, and tyrosinase inhibition activity of the extract samples were measured. Hunter Lightness values of laver, sea mustard, kelp, and fusiformis extracts were 82.88, 78.53, 83.04, and 78.11, respectively. The contents of total phenolic compounds of the seaweed extracts powder, laver, sea mustard, kelp, and fusiformis were 43.23, 11.59, 10.09, and 46.59 mg/g of sample, respectively. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the fusiformis extract was shown to be the highest value compared with other seaweed extracts. 2,2'-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) radical scavenging activities of laver, sea mustard, kelp, and fusiformis extracts were 258.00, 219.26, 95.77, and $1186.62{\mu}mol$ trolox equivalence per gram, respectively, at the 1,000 ppm level. TBARS value of oil emulsion, samples without extracts was higher than those of the samples prepared with laver and sea mustard extracts. The inhibition rates (%) of the mushroom tyrosinase of laver, sea mustard, kelp, and fusiformis extracts powder were 25.93, 26.32, 24.76 and 20.24% at 1,000 ppm, respectively. The results indicated that laver, sea mustard, kelp and fusiformis extracts possess biological activities such as antioxidant activity and tyrosinase inhibition effect.

• Journal of Mushroom
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• v.18 no.3
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• pp.244-253
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• 2020

The aim of this study was to investigate the biological activity of extracts obtained from the mycelium, sclerotium, and fruiting body of Wolfiporia cocos using different extraction solvents (hot water, 70% ethanol, and 70% methanol). Among the three developmental stages, the mycelium extracts showed the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ferric reducing antioxidant power (FRAP), nitrite scavenging activity, and total polyphenolic content. Among the extraction solvents in the context of the W. cocos mycelium, the DPPH radical scavenging activity, FRAP, and total polyphenol content in the hot-water extracts were significantly higher than those in the other extracts. In the case of the sclerotium, the reducing power, nitrite scavenging activity, and total polyphenol content were significantly higher in 70% ethanolic extracts. The fruiting body showed the highest DPPH radical scavenging activity, reducing power, nitrite scavenging activity, and total polyphenol content in the context of hot-water extraction. Moreover, the β-glucan content was significantly higher in the sclerotium versus the mycelium or fruiting body. The total amino acid and total essential amino acid contents were remarkably higher in the mycelium and fruiting body than in the sclerotium; of note, and arginine (Arg) and phenylalanine (Phe) were highly detected among the amino acid components.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.64-64
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• 2021

색과 향과 맛 및 기능성까지 뛰어난 대표적인 꽃차가 백목련이나 기능성에 관한 연구는 아직 매우 미미하다. 본 연구에서는 유리산소 소거능(DPPH)와 아질산염 소거능(Nitrite Scavenging Activity)이 백목련 꽃차가 제조된 2017년부터 2021년까지의 연도에 따라서 차이가 있는 지를 알아보고자 수행하였으며 그 결과는 다음과 같았다. 1. DPPH는 측정농도(0.5g/100ml=500ppm)에서 제조연도가 2017년 것은 87.8±0.0, 2018년 것은 87.5±0.1, 2019년 것은 85.6±0.6, 2020년 것은 88.5±0.2, 2021년 것은 86.1±0.1으로 2020년에 제조한 것이 가장 높은 함량을 보였다. RC50이란 소거능력이 50%를 환원시킬 수 있는 환원율을 의미하며 낮을수록 적은 양으로 환원이 가능하여 함량이 높다고 할 수 있으며 RC50값은 2017년이 140,2, 2018년이 165.0, 2019년이 178.6, 2020년이 112.2, 2021년이 151.4으로 2020년이 가장 뛰어난 활성을 보였다. 2. 아질산염 소거능은 측정농도(0.5g/100ml=500ppm)에서 제조연도가 2017년 것은 31.8±1.9, 2018년 것은 21.1±4.6, 2019년 것은 18.2±1.9, 2020년 것은 31.0±1.4, 2021년 것은 19.3±5.7의 수치를 보여 2017년과 2020년이 높은 활성을 보였다.

• Journal of Acupuncture Research
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• v.28 no.2
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• pp.117-123
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• 2011

목적 : 엘라스타제는 폐의 탄성 섬유를 파괴하여 폐기종과 폐고혈압을 유발하는 역할을 한다. 이에 본 연구는 문합약침액(文蛤藥鍼液)의 엘라스타제 억제효능과 항산화효능을 측정하여 폐기종과 폐고혈압 치료의 가능성을 확인하고자 실시하였다. 방법 : 문합약침액(文蛤藥鍼液)의 엘라스타제에 대한 억제효능과 di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH) 및 nitric oxide(NO) 자유기 소거능을 측정하였다. 결과 : 문합약침액(文蛤藥鍼液)은 엘라스타제 활성을 통계적으로 유의하게 억제하였고, DPPH 및 NO 자유기 소거능이 있음을 통계적으로 유의하게 확인하였다. 결론 : 본 연구를 통해 문합약침액(文蛤藥鍼液)의 엘라스타제 억제효능과 항산화 효능이 확인되었다. 본 연구 결과를 근거로 시험관 내 실험 이외의 추가적인 연구를 통해 폐기종과 폐고혈압 치료제로서 문합약침액(文蛤藥鍼液)의 활용을 고려해 볼 수 있을 것으로 기대한다.

• Food Science and Preservation
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• v.22 no.4
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• pp.577-584
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• 2015

The objective of this study was to investigate the antioxidant activity of imported tropical and subtropical fruits including dragon fruits, green kiwi, papaya, pineapple, pomegranate, and yellow mango. A seventy percent of ethanol extracts were prepared. Total phenolic content, DPPH (1,1-diphenyl-2-picryl hydrazyl)-, ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), superoxide anion radical scavenging activity, FRAP (ferric reducing antioxidant power), tyrosinase inhibitory activity, and reducing power were investigated for the comparisons of antioxidant activities. The phenolic content expressed as gallic acid equivalents (GAE) was found to be highest in pomegranate (12.22 mg GAE/g), followed by pineapple (3.77 mg GAE/g). Pomegranate and pineapple exhibited higher antioxidant activity than those of other fruits except for FRAP. DPPH and ABTS radical scavenging activity from pomegranate were 93.00%, and 98.98%, respectively, at a concentration of 5 mg/mL, which were equal to those of ascorbic acid used for a positive control.