• 한국식품위생안전성학회지
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• 제33권6호
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• pp.427-437
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• 2018

In this study, 23 ethanolic extracts from 20 medicinal plants were evaluated for biological activities. Results revealed that of 23 samples, seven samples have demonstrated good antimicrobial activity. Minimum inhibitory concentrations were 0.4-2.0 mg/mL, while minimum bactericidal concentrations were mostly high 0.8-2.0 mg/mL for selected seven samples. Five samples revealed > 70 mg gallic acid equivalent (GAE)/g of total phenolic contents. Among test samples, six samples exhibited > 80% inhibition of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and only two samples exhibited > 80% inhibition of 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. A total of five test samples revealed Trolox equivalent antioxidant capacity more than $1000{\mu}m/g$. The MTT assay indicated that eight test samples exhibited > 90% viability of murine macrophage cells (RAW 264.7) at $250{\mu}g/mL$ and suppressed iNOS mRNA expression at transcriptional level when stimulated by lipopolysaccharide (LPS). Some medicinal plants revealed promising results, and so they have prospective for further more inclusive studies.

• Biomolecules & Therapeutics
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• 제21권5호
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• pp.349-357
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• 2013

6'-O-galloylpaeoniflorin (GPF) is a galloylated derivate of paeoniflorin and a key chemical constituent of the peony root, a perennial flowering plant that is widely used as an herbal medicine in East Asia. This study is the first investigation of the cytoprotective effects of GPF against hydrogen peroxide ($H_2O_2$)-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated a significant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, $H_2O_2$-generated intracellular reactive oxygen species (ROS), the superoxide anion radical ($O_2^-$), and the hydroxyl radical (${\cdot}$OH). GPF also safeguarded HaCaT keratinocytes against $H_2O_2$-provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector against ROS-mediated oxidative stress.

• Biomolecules & Therapeutics
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• 제16권3호
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• pp.286-292
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• 2008

Strawberry is widely consumed in diet and has been attracted much attention due to its potential for human health benefits. Strawberry contains a diverse range of phytochemicals but the biological activities with molecular mechanisms are poorly elucidated yet. In this study, the effects of the extracts of strawberry (Maehyang cultivar) on antioxidant, antiinflammatory, and antiproliferative potential against various cancer cells were investigated. The strawberry extracts (SE) of Maehyang cultivar showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities. In addition, SE inhibited the growth of human colon (HCT-116), lung (A549), stomach (SNU-638) and fibrosarcoma (HT-1080) cancer cells. The strawberry extracts also exhibited the inhibitory effect on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and suppressed LPS-induced inducible nitric oxide synthase (iNOS) protein and mRNA expression in mouse macrophage RAW 264.7 cells. These findings suggest that the strawberry extracts (Maehyang cultivar) might have antioxidant, antiinflammotry, and anticancer activities.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.321-327
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• 2022

본 연구는 생김치, 적숙기 및 과숙기 상태의 세 가지 발효단계별 김치의 이화학적 특성과 항산화 활성에 대해 알아보았다. pH 및 산도 측정을 통해 생김치(pH 5.6, 산도 0.3%), 적숙기 김치(pH 4.3, 산도 0.64%), 과숙기 김치(pH 3.7, 산도 1.24%)를 시료로 사용하였다. 발효가 진행될수록 glucosinolate의 함량이 증가하였으며, 특히 생김치, 적숙기 김치, 과숙기 김치의 glucoraphanin 함량이 가장 높았다. 뿐만 아니라, 발효가 진행되면서 항산화 물질로 알려진 glucoraphanin, sulforaphane, 및 총 폴리페놀 함량이 증가하였다. 발효단계별 김치 시료의 항산화 활성을 측정한 결과, 100, 250, 500 ㎍/mL의 농도에서 생김치에 비해 적숙기와 과숙기 김치의 DPPH radical 소거능이 우수하게 나타났으며, ·OH radical 소거능을 측정하였을 때 모든 농도(100, 250, 500, 1000 ㎍/mL)에서 적숙기와 과숙기 김치가 생김치에 비해 우수한 소거능을 나타낸 것으로 보아 김치의 발효가 진행될수록 radical 소거능이 증가함을 알 수 있었다. 이는 생김치에 비해 발효과정을 거친 적숙기 및 과숙기 김치가 glucosinolate, sulforaphane, 총 폴리페놀 함량이 증가하여 항산화 활성이 높게 나타난 것으로 사료된다.

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.215-220
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• 2007

One of the important functions of skin is protection from harmful environments. Many studies have explored how to prevent skin from wrinkling and the occurrence of pigmentation changes. Skin wrinkling and pigmentation changes could be caused by unusual disruption of connective tissue, the formation of free radicals and ultraviolet radiation. In this study, extracts obtained from 254 different kinds of Jeju medicinal plants were screened for inhibitory effects on tyrosinase and elastase, and for free radical scavenging effects. Four herbs, Phormium tenax, Morus bombycis, Morus alba, and Cudrania tricuspidata, were potent inhibitors of tyrosinase ($IC_{50}$ values 4.62, 5.46, 8.17, and 64.17 ${\mu}g$/mL, respectively). Aleurites fordii [$IC_{50}$: 5.29 ${\mu}g$/mL, 1,1-diphenyl-2-picrylhydrazyl (DPPH)], Distylium racemosum ($IC_{50}$: 6.14 ${\mu}g$/mL), Acer palmatum ($IC_{50}$: 5.44 ${\mu}g$/mL), and Spiraea salicifolia ($IC_{50}$: 5.25 ${\mu}g$/mL) showed good antioxidative effects. Furthermore, Distylium racemosum ($IC_{50}$: 7.51 ${\mu}g$/mL), Diospyros kaki ($IC_{50}$: 15.1 ${\mu}g$/mL), Cornus macrophylla ($IC_{50}:$ 16.59 ${\mu}g$/mL), and Psidium guajava ($IC_{50}$: 40.25 ${\mu}g$/mL) exhibited potent inhibitory effects on elastase. These results suggest that medicinal plants possessing several biological activities may be potent inhibitors of the processes involved in pigmentation increases and aging. Further investigations will focus on in vivo assays and on the chemical identification of the major active components responsible for whitening and anti-aging activity in the screened efficacious extracts.

• 대한본초학회지
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• 제34권3호
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• pp.1-7
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• 2019

Objectives : Amyloid ${\beta}(A{\beta})$ could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in $A{\beta}$-induced an AD model. Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of $A{\beta}$ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 mg/kg AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test. Results : The total polyphenol and flavonoids contents of the AK extract were $88.73{\pm}6.36mg$ gallic acid equivalent/g, $84.21{\pm}5.04mg$ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, $1000{\mu}g/m{\ell}$) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the $A{\beta}$ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and $A{\beta}$ 1-42-induced shorting of the step-through latency in the passive avoidance test. Conclusions : The findings suggest that AK indicated the antioxidant protective effects against $A{\beta}$-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.

• 대한화장품학회지
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• 제33권3호
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• pp.165-173
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• 2007

선구 연구로부터 저자들은 제주 자생 식물 추출물의 항산화 및 세포보호 효과에 대한 결과의 일부를 이미 보고하였으며, 본 연구에서는 제주도에서 자생하는 나머지 37종의 식물 추출물에 대한 항산화, 미백 그리고 주름생성억제 효과를 측정하였다. 항산화 효과는 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)을 이용한 free radical 소거 활성 측정, $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종으로 인한 luminol의 화학발광을 이용한 소거 활성, 사람 적혈구를 대상으로 하여 rose-bengal로 증감된 활성산소에 대한 세포보호 효과를 측정하였다. 미백 및 주름억제 효과측정으로는 각각 tyrosinase, elastase의 활성 저해 효과를 측정하였다. 실험결과, free radical의 소거 활성($FSC_{50}$)은 소귀나무 수피(Myrica rubra, 5 ${\mu}g/mL$), 광대싸리 수피(Securinega suffruticosa, 8 ${\mu}g/mL$)에서 높게 나타났고, 활성산소 소거 활성($OSC_{50}$)은 상수리나무 잎(Quercus acutissima)과 광대싸리 수피(Securinega suffruticosa)에서 0.009 ${\mu}g/mL$로 높게 나타났으며, 세포보호 효과(${\tau}_{50}$)는 50 ${\mu}g/mL$에서 광대싸리 수피(Securinega suffruticosa, 895 min), 버드나무 줄기(Salix koreensis, 640 min)에서 크게 나타났다. 200 ${\mu}g/mL$에서 tyrosinase의 활성 저해 효과($IC_{50}$)는 소귀나무 수피(Myrica rubra, 77.8%), elastase의 활성 저해 효과($IC_{50}$)는 버드나무 줄기(Salix koreensis, 76.2%)에서 큰 효과가 나타났다. 결론적으로 광대싸리 수피, 소귀나무 수피, 상수리나무 잎, 버드나무 줄기, 동백나무 잎/줄기 추출물은 기능성 화장품 제조를 위한 원료로 사용하기에 충분한 가능성이 있음을 확인할 수 있었으며, 향후에 제품화를 위하여 좀 더 다양한 연구들이 필요하다고 사료된다.

• 대한화장품학회지
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• 제31권1호
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• pp.73-78
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• 2005

본 연구는 천연물로부터 기능성화장품의 소재로 이용할 수 있는 새로운 항산화제를 개발하기 위하여 정선산 오가피나무 추출물의 항산화 효과를 측정하였다. 통상적 항산화력 평가기법인 DPPH 라디칼 소거능 평가 방법은 천연물의 항산화능을 평가하기에 가장 쉬운 방법중에 하나이나 생체내에서 발생되는 특정 라디칼에 대한 항산화능을 평가하기에는 부적합하여 본 연구진은 전사상자성공명법이라는 방법을 이용하여 특정 라디칼에 대한 오가피나무 추출물의 항산화능을 평가하였다. 전자상자성공명법을 이용한 항산화능 평가 결과 오가피나무의 추출물은 유해산소종 및 유해질소종에 대한 매우 우수한 항산화능을 가지고 있음이 밝혀졌으며, 이리한 결과로 보아 오가피나무 추출물은 새로운 항산화제 소재 및 피부내의 산화적 스트레스 방지목적의 기능성 화장품소재로서의 개발가능성이 매우 높음을 알 수 있었다.

• 대한한방부인과학회지
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• 제22권2호
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• pp.119-131
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• 2009

Purpose: The depression accompanied with menopuase shows the relation with the dopamine secretion. These studies were undertaken to evaluate the anti- oxidative and neuroprotective effects of Bunsimgieum(BSGE) on dopaminergic neurons. Methods: To estimate the antioxidant effects, we carried out 1.1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3-ethylbenzothiazoline -6-sulfonic acid (ABTS) radical cation decolorization assay, and measurement of total polyphenolic content. To evaluate neuroprotective effect of BSGE in vitro, We performed thiazolyl blue tetrazolium bromide (MTT) assay, reactive oxygen species (ROS) creation in SH-SY5Y. Tyrosine hydroxylase (TH) immunocytochemistry, nitric oxide (NO) assay, and TNF-${\alpha}$ assay in primary rat mesencephalic dopaminergic neurons. Results: The DPPH free radical and the ABTS radical cation inhibition activities were increased at a dose dependent manner. Total polyphenolic content was 0.83%. In SH-SY5Y culture, BSGE significantly increased the decreased cell viability by 6-OHDA at the concentrations of 10${\mu}$g/m${\ell}$ in pre-treatment group, 0.1-200${\mu}$g/m${\ell}$ in post-treatment group. The production of ROS induced by 6-OHDA was significantly inhibited in BSGE treated group. In mesencephalic dopaminergic cell culture, the BSGE group reduced the dopaminergic cell loss against 6-OHDA toxicity and the production of No and TNF-${\alpha}$ at the concentration of 5${\mu}$g/m${\ell}$. Conclusion: These results shows that BSGE has antioxidant and neuroprotective effects in the dopaminergic cells through decreasing the production of ROS, NO and TNF-${\alpha}$ which can cause many neurodegenerative changes in brain cell. We suggest that BSGE could be useful for the treatment of postmenopausal depression related with the decrease of dopamine.

• 한국식품영양과학회지
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• 제37권1호
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• pp.1-7
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• 2008

본 연구에서는 산수유 에탄올 추출물의 항산화능, 항돌연변이 및 항암활성을 분석하여 새로운 생리활성 물질의 탐색과 고부가가치의 기능성식품으로서의 이용 가능성을 알아 보고자 하였다. 산수유 에탄올 추출물의 전자공여능은 농도 의존적으로 증가하여 500 ppm의 농도에서는 합성항산화제인 BHT보다 높았고 천연항산화제인 L-ascorbic acid와 유사하였다. 뿐만 아니라, S. Typhimurium TA100에 대해 산수유 에탄올 추출물은 직접돌연변이원인 4-NQO 사용 시 항돌연변이 효과가 우수한 것으로 나타났다. 또한, 산수유 에탄올 추출물은 $700{\mu}g/plate$ 첨가하였을 때 인체 간암 세포와 자궁경부암 세포의 증식을 모두 78% 이상 억제하였다. 산수유 에탄올 추출물은 비교적 높은 함량의 폴리페놀과 플라보노이드를 함유하고 있어, 이들 물질이 항산화와 항암효과에 영향을 미칠 것으로 사료되었다. 그러나 산수유에는 비타민 C를 비롯한 다른 항산화 성분도 함유되어 있으므로 이들의 항산화성도 추출물의 항산화력에 영향을 주었을 것으로 생각된다. 결론적으로, 산수유 에탄올 추출물은 항산화능과 항돌연변이 활성이 있었으며 인체 간암세포, 자궁경부암 세포 및 대장암 세포에 대해 항암 활성이 있는 것으로 나타나 기능성식품의 소재로서의 이용 가능성을 보여주었다.