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Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures

  • Matos, Rosan Barbosa De;Braga-de-Souza, Suzana;Pitanga, Bruno Pena Seara;Silva, Victor Diogenes Amaral Da;Jesus, Erica Etelvina Viana De;Pinheiro, Alexandre Morales;Costa, Maria De Fatima Dias;El-Bacha, Ramon Dos Santos;Ribeiro, Catia Suse De Oliveira;Costa, Silvia Lima
    • Parasites, Hosts and Diseases
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    • v.52 no.6
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    • pp.613-619
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    • 2014
  • Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.

Soil Erosion Assessment Tool - Water Erosion Prediction Project (WEPP) (토양 침식 예측 모델 - Water Erosion Prediction Project (WEPP))

  • Kim, Min-Kyeong;Park, Seong-Jin;Choi, Chul-Man;Ko, Byong-Gu;Lee, Jong-Sik;Flanagan, D.C.
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.4
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    • pp.235-238
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    • 2008
  • The Water Erosion Prediction Project (WEPP) was initiated in August 1985 to develop new generation water erosion prediction technology for federal agencies involved in soil and water conservation and environmental planning and assessment. Developed by USDA-ARS as a replacement for empirical erosion prediction technologies, the WEPP model simulates many of the physical processes important in soil erosion, including infiltration, runoff, raindrop detachment, flow detachment, sediment transport, deposition, plant growth and residue decomposition. The WEPP included an extensive field experimental program conducted on cropland, rangeland, and disturbed forest sites to obtain data required to parameterize and test the model. A large team effort at numerous research locations, ARS laboratories, and cooperating land-grant universities was needed to develop this state-of-the-art simulation model. The WEPP model is used for hillslope applications or on small watersheds. Because it is physically based, the model has been successfully used in the evaluation of important natural resources issues throughout the United State and in several other countries. Recent model enhancements include a graphical Windows interface and integration of WEPP with GIS software. A combined wind and water erosion prediction system with easily accessible databases and a common interface is planned for the future.

Methodologies for the Determination of Endogenous Phosphorus Losses in Growing Pigs

  • Alves, Debora Aline;da Rocha, Leonardo Tombesi;dos Santos Camargo, Claudiele Aparecida;Figueiredo, Andriele Medianeira;Ceron, Marcos Speroni;Lucca, Walter;Zanella, Irineo;de Oliveira, Vladimir
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.11
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    • pp.1632-1638
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    • 2016
  • A study was conducted to compare the endogenous basal losses of phosphorus (EBLP) in pigs fed diets containing gelatin (GEL) or spray-dried porcine plasma (SDPP) as protein sources and to determine the standardized total tract digestibility (STTD) of phosphorus (P) in SDPP. The trial was carried out at the Federal University of Santa Maria, Brazil. Twelve castrated pigs with an initial body weight of 55 kg were individually allotted in metabolic crates during two 12-day periods, each with 7 days of adaptation and 5 days of total fecal collection. The beginning and the end of the collecting periods were determined according to the marker-to-marker approach, using ferric oxide as an indigestible marker. Pigs were submitted to four semi-purified diets, one being a P-free diet with 30% of GEL as the protein source and three were diets with 10%, 20%, and 30% inclusion of SDPP respectively. Data were subjected to analysis of variance and the model included the effects of period, animal and treatments; the results of the three diets with increased levels of SDPP were subjected to linear regression analysis. The intercept of the relation of between ingested P and absorbed P represented the EBLP, while the slope indicated the STTD of P in SDPP. The EBLP means obtained by P-free diet and regression method were compared with the Student t test. The EBLP were 128.95 mg/kg dry matter intake (DMI) and 153.63 mg/kg DMI (standard error = 77.0; p<0.06) using the P-free diet with GEL as the protein source and the regression method, obtained with diets containing increased levels of SDPP, respectively. The apparent digestibility of P was 87.9%, 94.2%, and 92.9% for the treatments containing 10%, 20%, and 30% inclusion of SDPP, respectively. The estimated STTD of P obtained with the linear regression was 97.4%. When the EBLP estimated by the P-free diet was used to corrected the apparent digestibility of P in diets containing SDPP, the STTD of P in SDPP was 96.9%, 98.8%, and 95.9% for 10%, 20%, and 30% SDPP, respectively. Therefore, it can be concluded that SDPP can replace GEL to estimate the endogenous losses of P. In addition, the STTD of P in SDPP estimated with the P-free diet was 97.2% and it was 97.4% by the regression method, utilizing SDPP.

Effects of processing, particle size and moisturizing of sorghum-based feeds on pellet quality and broiler production

  • da Silva, Patricia Garcia;Oliveira, Luana Martins Schaly;de Oliveira, Nayanne Rodrigues;de Moura, Fabio Ataides Junior;Silva, Maura Regina Sousa;Cordeiro, Deibity Alves;Minafra, Cibele Silva;dos Santo, Fabiana Ramos
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.1
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    • pp.98-105
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    • 2018
  • Objective: This study aimed to assess the effect of pelleted and expanded sorghum-based feeds prepared with different moisture levels and particle size of ingredients on metabolizable energy, ileal digestibility of amino acids and broiler performance. Methods: The experiment was performed with 720 male broiler chicks of the Cobb strain, with treatments of six replications, with 15 birds each; they were arranged in a completely randomized design and $2{\times}2{\times}2$ factorial scheme (pelleted or expanded feed processing, 0.8% or 1.6% moisture addition in the mixer, and particle size of 650 or 850 microns). Results: Higher pellet quality (pellets, % and pellet durability index [PDI]) was obtained in expanded diets and inclusion of 1.6% moisture. The particle size of 850 microns increased the PDI of final diet. All studied treatments had no significant effect on weight gain and broiler carcass and cut yields. Lower feed conversion occurred for birds fed pelleted feed at 42 d. The highest apparent metabolizable energy (AME) and apparent metabolizable energy corrected to zero nitrogen balance (AMEn) values of feed in the initial rearing phase (10 to 13 days) were observed in birds fed pelleted feed or for feed prepared with 1.6% moisture. The highest ileal digestibility coefficients of amino acids were obtained with the consumption of pelleted feed prepared with a particle size of 650 microns and 1.6% moisture. Conclusion: Pelleted feed prepared with a milling particle size of 650 microns and 1.6% moisture provided increased ileal digestibility of amino acids and AMEn in the starter period. However, the expanded feed improved pellet quality and feed conversion of broilers at 42 days of age. We conclude that factors such as moisture, particle size and processing affect the pellet quality, and therefore should be considered when attempting to optimize broiler performance.

Design of a Holter Monitoring System with Flash Memory Card (플레쉬 메모리 카드를 이용한 홀터 심전계의 설계)

  • 송근국;이경중
    • Journal of Biomedical Engineering Research
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    • v.19 no.3
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    • pp.251-260
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    • 1998
  • The Holter monitoring system is a widely used noninvasive diagnostic tool for ambulatory patient who may be at risk from latent life-threatening cardiac abnormalities. In this paper, we design a high performance intelligent holter monitoring system which is characterized by the small-sized and the low-power consumption. The system hardware consists of one-chip microcontroller(68HC11E9), ECG preprocessing circuit, and flash memory card. ECG preprocessing circuit is made of ECG preamplifier with gain of 250, 500 and 1000, the bandpass filter with bandwidth of 0.05-100Hz, the auto-balancing circuit and the saturation-calibrating circuit to eliminate baseline wandering, ECG signal sampled at 240 samples/sec is converted to the digital signal. We use a linear recursive filter and preprocessing algorithm to detect the ECG parameters which are QRS complex, and Q-R-T points, ST-level, HR, QT interval. The long-term acquired ECG signals and diagnostic parameters are compressed by the MFan(Modified Fan) and the delta modulation method. To easily interface with the PC based analyzer program which is operated in DOS and Windows, the compressed data, that are compatible to FFS(flash file system) format, are stored at the flash memory card with SBF(symmetric block format).

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GIS-based Land Suitability Assessment for Pears (Pyrus) Using Soil and Climate Conditions in South Korea (GIS 기반의 토양 및 기후조건 통합 배 과수의 적지 평가)

  • Kim, Hojung;Shim, Kyo-Moon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.4
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    • pp.337-346
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    • 2019
  • Land suitability assessments for agricultural crop production determine the suitability of an area in terms of crop yields. We employed the maximum limiting characteristic method (MLCM) and an analytic hierarchy process (AHP) to assess land suitability for Pears (Pyrus) in all Korean provinces (Dos). In general, suitability grades according to the MLCM were much lower than those according to the AHP. The MLCM determined that suitable areas (S1, S2, and S3) exist on 19.55% of the land, whereas 99.08% of the land was classified as suitable by the AHP. Based on information on pear farms in each Korean province in 5 years (1982, 1987, 1992, 1997, 2002, and 2007), the proportion of suitable land area according to the MLCM in each province as compared to all suitable areas was closer to the proportion of areas covered by pear farms in the 5 years in each province and also to the proportion of pear farm areas in 2007 in each province. Based on the assumption that if a province has more suitable areas for pears, more pears will be cultivated in that province, the results of the MLCM can be regarded as more accurate than those of the AHP.

Characterization of Tifton 85 bermudagrass haylage with different layers of polyethylene film and storage time

  • Nath, Caroline Daiane;Neres, Marcela Abbado;Scheidt, Kacia Carine;Bersot, Luciano dos Santos;Sunahara, Samantha Mariana Monteiro;Sarto, Jaqueline Rocha Wobeto;Stangarlin, Jose Renato;Gomes, Simone Damasceno;Sereno, Mallu Jagnow;Perin, Ana Paula
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1197-1204
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    • 2018
  • Objective: The objective was to characterize the fermentative and microbiological profile of Tifton 85 bermudagrass haylage with different layers of polyethylene film and storage time. Methods: The experimental design consisted of a randomized block design with four and six wrapping layers (100 and 150 microns in total. respectively) allocated in the main plots, through repeated measures analysis (30, 60, and 90 days of storage) with four replicates. Results: The storage time and number of wrapping layers did not show changes in the population of Clostridium and lactic acid bacteria. A decrease was observed in the enterobacteria population with an increase in the storage period in the two wrapping layers studied. Upon opening of the haylage at 30 days, the population of Bacillus was lower in haylages made with six layers of wrapping (3.63 log colony forming units/g). No growth of Listeria sp. or Salmonella sp. was observed during the experimental period. The fungal genera with a greater occurrence were Penicillium sp. and Fusarium sp. The following mycotoxins were not detected: ochratoxin A, fumonisins, and zearalenone. Relative to the organic butyric, propionic, and acetic acids, the haylages presented a low concentration of lactic acid; this may have prevented a drop in the pH, which was high when the silos were opened (5.4). The levels of ammoniacal nitrogen and soluble carbohydrates presented no variation among the number of wrapping layers, with an overall average of 35.55 and 38.04 g/kg. Conclusion: Tifton 85 bermudagrass haylage wrapped with four and six layers presented adequate fermentation and microbiological characteristics in the evaluated periods.

The Detection of Signaling Dos on 4G LTE Cellular Network (4G LTE 이동통신망에서의 시그널링 DoS 탐지 기술)

  • Jang, Woung;Kim, Se-Kwon;Oh, Joo-Hyung;Im, Chae-Tae
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.146-149
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    • 2013
  • For in recently years, global cellular network service is changing rapidly to 4G. However, the fast introducing of 4G has been going with not enough research about security threat, it could be many kind of vulnerability. Therefore the research about security threat on 4G network is ongoing in external countries, but not sufficient in domestic. particularly in domestic situation of rapidly increased subscribers, The security threats which are hindering stability and usability could make a fatal effect on many users. 4G network should be considered about the feature of mobile network to protect 4G network stable. Mobile network has limited radio resources, it releases the radio resource which is not used in selected time and reallocate when detected the data transmission. Many signaling messages are transferred in the network entities to allocate or release the radio resource. In this paper, it will be introduced the technology to detect signaling DoS traffic hindering the stability and usability of network entities managing the radio resources by huge signaling message from the repetitive wireless connection/release message.

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Preparation and Characterization of Domestic Alkali-Surfactant-Polymer Solution for Enhanced Oil Recovery (국내산 계면활성제를 이용한 오일회수증진용 알칼리-계면활성제-폴리머용액의 제조 및 특성평가)

  • Lee, Sang Heon;Kim, Sang Kyum;Park, Ji Yun;Lee, Do Kyun;Hwang, Soon Choel;Bae, Wisup;Kim, In Won;Rhee, Young Woo
    • Clean Technology
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    • v.19 no.4
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    • pp.481-486
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    • 2013
  • Alkali-surfactant-polymer (ASP) solution was manufactured by using the domestic surfactants for enhanced oil recovery. Domestic surfactants such as linear alkylbenzene sulfonic acid (LAS) and dioctyl sulfosiuccinate (DOSS) were used. This surfactants were purchased from AK chemtech Co., Ltd. (Korea). LAS and DOSS were blended and the ratio of LAS to DOSS are 1:1 and 2:1. Decane was used as a model compound of the crude oil. Surfactant solution and decane were blended to analyze microemulsion. Brine-oil-surfactant are mixed at varying concentration of brine from 0.8 to 3.6 wt.%. Increasing salinity causes the phase transition of microemulsion from water to middle to oil. Also, by measuring the surface tension and interfacial tension using pendent drop tensiometer and Huh's equation optimal ratio of the ASP solution was determined.

A Study on the Performance Improvement of the Security Transmission Using the SSFNet (SSFNet을 이용한 보안전송 성능개선에 관한 연구)

  • Ryu, Jung-Eun;Ryu, Dong-Ju;Lee, Taek-Hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.2
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    • pp.811-815
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    • 2005
  • IPSec(Internet Protocol Security) is a framework for a set of protocols for security at the network or packet processing layer of network communication. IPSec is providing authentication, integrity and confidentiality security services. The specifications for Internet Key Exchange(IKEv1) were released to the world. Some criticisms of IKEv1 were that it was too complex and endeavored to define too much functionality in one place. Multiple options for multiple scenarios were built into the specification. The problem is that some of the included scenarios are rarely if ever encountered. For IPsec to work, the sending and receiving devices must chare a Public Key. This is accomplished through a protocol known as Internet Security Association and Key Management Protocol/Oakley(ISAKMP/Oakley), which allows the receiver to obtain a public key and authenticate the sender using digital certificates. This thesis is a study on the performance improvement of the security transmission using the SSFNet(Scalable Simulation Framework Network Models)

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