• The Korean Journal of Ecology
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• 제24권6호
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• pp.385-394
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• 2001

An attempt to clarify the landscape structure of urban areas was carried out in the greenbelt around Seoul, Korea's metropolis. By means of aerial photographs and a field survey, a vegetation map including land-use pattern was made. Landscape structure was described by analyzing this vegetation map and the results of phytosociological survey. Landscape element types identified were (1) secondary forest, (2) plantation, (3) cultivated field, (4) urbanized area, (5) graveyard, and (6) bare rock. Vegetation units, resulting from the phytosociological analysis, included Quercus mongolica, Q. variabilis, Q. acutissima, Pinus densiflora, Q. aliena, and Alnus japonica communities. Plantations were composed of Robinia pseudoacacia, Populus tomentiglandulosa, P. rigida, Larix leptolepis, P. koraiensis, and Castanea crenata stands. Patches near to human settlements in the lower zones of the mountains were fragmented and small but they became larger towards the higher mountain zones. On the other hand, the number of patches was fewer and their size was larger in Mt. Cheonggye more distant from the principal residential area, larger in size, and higher in elevation compared with the other 2 mountains, Mt. Daemo and Mt. Acha. Floristic composition of Mongolian oak(Q. mongolica) stand distributing in the upper part of each mountain, in which artificial interference is rare, showed a difference among those study areas different in parent rock and disturbance regime. But that of black locust(R. pseudoacacia) stand located in lowland of mountainous area, in which artificial interference is frequent was similar to each other. As the results of analyses on the frequency distribution of diameter classes of major species, dominant landscape elements, Mongolian oak forest showed different responses depending on artificial interference as continuous maintenance and retrogressive succession in the sites far from and near to the residential areas, respectively. On the other hand, black locust stands showed a probability to be restore to the native oak forest through progressive succession.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.449-458
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• 2019

Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10-20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.897-910
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• 2022

Bovine fecal microbiota is important for host health and its composition can be affected by various factors, such as diet, age, species, breed, regions, and environments. The objective of this study was to evaluate the impact of diet and gender on fecal microbiota in Korean native Hanwoo cattle. The 16S rRNA gene amplicon sequencing of fecal microbiota was conducted from 44 Hanwoo cattle divided into four groups: (1) 11 heifers fed an oat hay plus total mixed ration (TMR) diet for breeding (HOTB), (2) 11 heifers fed an early fattening TMR diet (HEFT), (3) 11 steers fed the early fattening TMR diet (SEFT), and (4) 11 steers fed the late fattening TMR diet (SLFT). Firmicutes and Bacteroidota were the first and second most dominant phyla in all the samples, respectively. The Firmicutes/Bacteroidota (F/B) ratio associated with feed efficiency was significantly greater in the SLFT group than in the other groups. At the genus level, Romboutsia, Paeniclostridium, and Turicibacter were the most abundant in the SLFT while Akkermansia, Bacteroides, and Monoglobus were the most abundant in the HOTB group. Although the same early fattening TMR diet was fed to Hanwoo heifers and steers, Marvinbryantia and Coprococcus were the most abundant in the HEFT group while Alistipes and Ruminococcus were the most abundant in the SEFT group. Shannon and Simpson diversity indices were significantly lower in the SLFT group than in the other groups. Distribution of fecal microbiota and functional genetic profiles were significantly different among the four treatment groups. The present study demonstrates that different diets and genders can affect fecal microbiota and the F/B ratio may be associated with feed efficiency in Hanwoo cattle. Our results may help develop strategies to improve gut health and productivity through manipulation of fecal microbiota using the appropriate diet considering Hanwoo cattle gender.

• Mycobiology
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• 제43권4호
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• pp.373-383
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• 2015

Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

• 생태와환경
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• 제56권3호
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• pp.207-217
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• 2023

본 연구는 2023년 5~6월 낙동강하구에 생육하는 잘피 분포 현황을 조사하기 위해 실시하였다. 잘피 서식 면적을 조사하기 위해 낙동강하구를 7개 구역으로 구분하여 드론을 이용한 항공촬영을 실시하여 잘피 서식을 확인한 후 조간대에서는 도보로, 조하대에서는 선박 및 잠수조사로 GPS 트랙킹을 실시하였다. 잘피 현황을 분석하기 위해 각 구역에 대표적으로 출현하는 잘피의 종별 형태적 특성, 생육밀도와 생체량을 측정하였다. 낙동강하구에는 애기거머리말, 거머리말, 줄말과 게바다말이 출현하였고, 각 종의 분포 면적은 각각 338.2 ha, 92.9 ha, 0.9 ha와 1.4 ha로 총 잘피 서식지 면적은 432.5 ha로 조사되었다. 애기거머리말은 낙동강하구 대부분의 사주와 갯벌에 넓게 분포하였고, 거머리말은 눌차도, 진우도와 다대동에 서식하였다. 줄말은 을숙도와 명지갯벌의 애기거머리말 서식지 내에 출현하였고, 게바다말은 다대동의 암반에 생육하였다. 애기거머리말, 거머리말, 줄말과 게바다말의 생육 밀도는 각각 4,575.8±338.3 shoots m^-2, 244.8±12.0 shoots m^-2, 11,302.1±290.0 shoots m^-2와, 2,862.5±153.5 shoots m^-2였다. 생체량은 각각 239.7±18.5 gDW m^-2, 362.3±20.5 gDW m^-2, 33.3±1.2 gDW m^-2와, 1,290.0±37.0 gDW m^-2였다. 본 연구 결과 낙동강하구에는 애기거머리말이 우점하였고, 특히, 을숙도, 대마등과 명지갯벌의 애기거머리말 서식지는 국내 최대 규모로 형성되었음을 알 수 있었다. 낙동강하구는 생태적, 환경적, 경제적 가치가 높은 중요한 곳으로, 이곳에 생육하는 잘피의 지속적인 조사와 관리가 필요하다.

• 한국작물학회지
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• 제64권3호
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• pp.246-268
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• 2019

Soybean [Glycine max (L.) Merr.] is a species of legume native to East Asia. The interactions between climatic conditions and genetic characteristics are known to affect the agricultural performance of soybean. Therefore, the present investigation was conducted to identify the main elements affecting the agricultural performances of 11 soybean varieties/lines from China [Harbin ($45^{\circ}12^{\prime}N$), Yanji ($42^{\circ}53^{\prime}N$), Dalian ($39^{\circ}30^{\prime}N$), Qingdao ($36^{\circ}26^{\prime}N$)] and the Republic of Korea [Suwon ($37^{\circ}16^{\prime}N$), and Jeonju ($35^{\circ}49^{\prime}N$)]. The days to flowering (DTF) of soybeans with the e1-nf and e1-as alleles and the E1e2e3e4 genotype, except in 'Keumgangkong', 'Tawonkong', and 'Duyoukong', were relatively short compared to those of soybeans with other alleles. Although DTF of the soybeans was highly correlated with all climatic conditions [negative: precipitation, average temperature (AVT), accumulated temperature; positive: day-length (DL)], days to maturity and 100-seed weight of the soybeans showed no significant correlation with any climatic conditions. The soybeans with a dominant Dt1 allele, except 'Tawonkong', had the longest stem length (STL). Moreover, STL of the soybeans grown in the test fields showed a positive correlation with only DL; however, the results of our chamber test that was conducted to complement the field tests showed that STL of soybean was positively affected by AVT and DL. Although soybean yield (YLD) showed positive correlations with latitude and DL (except L62-667, OT89-5, and OT89-6), the response of YLD to the climatic conditions was cultivar-specific. Our results show that DTF and STL of soybeans grown in six different latitudes are highly affected by DL, and AVT and genetic characteristic also affect DTF and STL.

• 생태와환경
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• 제41권3호
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• pp.402-411
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• 2008

남조류 제어능이 밝혀진 국내산 말조개와 논우렁이를 금강수계에서 채집하여, 저온기 부영양 호수에서 우점하는 규조류에 대한 섭식특성을 파악하고자, 두 패류를 단독 또는 혼합적용하고, 수조내 엽록소-a, 조류현존량, 섭식율 및 배설물 생산량의 변화를 각각 조사하였다. 섭식 실험은 패류밀도에 따라 말조개 단독처리군 5가지, 논우렁이 단독처리군 5가지, 두 패류의 혼합 처리군 4가지 등으로 구분하여 각각 3회씩 반복 실험하였다. 분석결과, 모든 패류 처리군에서 엽록소-a (또는 조류현존량 농도)가 뚜렷하게 감소하였으며, 말조개와 말조개 밀도가 높은 혼합군에서 조류제어율이 높았다. 각 출현종에 대한 패류의 조류제어능은 Synedra ulna, Scenedesmus sp., Ankistrodesmus falcatus 등은 말조개 밀도가 높을수록, Diatoma vulgare는 혼합 처리군에서 각각 높게 제어된 반면, Cryptomonas ovata는 고밀도 논우렁이 처리군에서 오히려 20% 성장을 유도하였다. 패류의 섭식율은 패류와 처리시간에 따라 차이를 보였는데, 초기에는 운동성이 좋은 논우렁이가 강한 섭식을 보인 반면, 시간이 경과함에 따라 말조개 및 고밀도 말조개 혼합 처리군에서 섭식율이 높았다. 패류의 배설물 생산은 혼합 처리군에서 가장 높았으며, 60 ${\mu}m$ 이상의 크기에서 높게 나타났다. 본 연구에서 국내산 말조개와 논우렁이를 단독 또는 혼합 적용시 모든 처리군에서 규조류가 뚜렷하게 감소하였으며, 말조개나 말조개 혼합 비율이 높을수록 제어율이 높았다. 따라서 앞으로 패류들의 현장적용 기술이 개발된다면 저온기 규조류 대발생의 생태친화적 제어가 가능할 것으로 사료되었다.

• 한국자원식물학회지
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• 제31권4호
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• pp.396-418
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• 2018

본 연구는 사무산의 관속식물상을 밝히고 주요 식물을 조사하였다. 2014년 6월부터 2015년 10월까지 총 9회에 걸쳐 현지조사를 실시한 결과 관속식물은 108과 306속 424종 8아종 48변종 4품종 1교잡종으로 총 502분류군이 확인되었다. 식물종다양성이 높은 과는 국화과(70분류군), 화본과(52분류군), 장미과(30분류군), 콩과(28분류군), 사초과(20분류군)이었다. 한반도 고유종은 10분류군이며, IUCN 평가기준에 따른 적색목록식물은 14분류군으로 확인되었다. 식물구계학적 특정식물은 V등급 5분류군, IV등급 11분류군, III등급 14분류군, II등급 10분류군, I등급 21분류군으로 총 61분류군이 관찰되었다. 외래식물은 43분류군으로 귀화율 8.6%, 도시화지수 13.4%로 나타났다. 삼림식생은 소나무림과 굴참나무림, 상수리나무림, 신갈나무림, 느티나무림, 아까시나무림으로 대별된다. 삼림의 수직구조는 안정적이며, DBH-Class 분석 결과는 교목층의 우점종이 한동안 유지될것으로 나타났다. 사무산은 식물종다양성이 높고 고유종, 희귀식물, 호석회성식물 등 주요 식물들이 다수 분포하며, 식물지리학적 가치가 높은 것으로 생각된다. 이러함에도, 인위적인 교란과 훼손에 대한 어떠한 보호조치도 없기 때문에 생물자원이 지속적으로 소실되고 있다. 따라서 산림식생 보전을 위한 대책이 마련될 필요가 있다.