• 생태와환경
• /
• 제43권2호
• /
• pp.338-344
• /
• 2010

상수원 및 수계의 수질관리 업무를 효율적이고 능동적으로 수행하고, 수질오염사고 발생시 신속한 대응조치를 할 수 있는 조기경보체계 구축을 위하여 주요 상수원 및 하천 등에 수질자동측정망을 설치하여 운영하고 있다. 수온, pH, EC, DO, TOC 항목에 대한 정도평가를 실시하여, 수질자동 측정 자료에 대한 정확도를 확보하여 신뢰도를 제고하고자 하였다. 수질자동측정기기와 수동측정기기에 대한 측정값을 비교한 결과, 대체로 일반항목(수온, pH, EC, DO) 및 TOC의 상대정확도가 20% 이내의 목표를 만족하였다. 그러나 수질자동측정소의 저류수조의 측정기기(내부)와 채수지점의 측정기기(외부)의 측정값 차이를 분석한 결과, 채수거리가 먼 측정소의 DO에 대한 내부-외부의 상대정확도는 목표값을 다소 벗어나는 것으로 나타났다. 채수관로에 조류 및 미생물 부착에 의한 오염이 원인으로 판단되어 역세척하여 측정한 결과, 측정된 DO 농도는 최대 53%까지 상승하는 효과를 나타냈다. 따라서 채수관로 관리가 수질자동측정 자료의 신뢰성을 확보하는데 중요함을 알 수 있었다.

• 한국물환경학회지
• /
• 제31권2호
• /
• pp.87-93
• /
• 2015

In this study, the inhibitory effect of toxic metals was investigated on the activated sludge of the municipal sewage treatment plant. The allowable concentration of toxic metals was also estimated for the stable operation of the biological treatment process. The single and mixture toxicity of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ were evaluated for the activated sludge microorganisms. As a result, nitrifying microorganisms were more susceptible than heterotrophic microorganisms. $IC_{10}$ (Inhibition Concentration of 10%) of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ for the nitrifying microorganisms was 3 mg/L, 7 mg/L and 25 mg/L, respectively. The mixture toxicity showed three times more sensitive than the single toxicity. The concentrations of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ to minimize the inhibitory effect on organic matter removal and nitrification in batch experiments were found to be 1.3 mg/L, 2.5 mg/L and 6.3 mg/L.

• Journal of Microbiology and Biotechnology
• /
• 제22권6호
• /
• pp.818-825
• /
• 2012

Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI database was observed. Certain parameters for immobilization of the purified keratinase on chitosan beads were investigated. The production of the chitosan beads was optimized using factorial design and surface response techniques. The optimum chitosan bead production for protease immobilization was a 20 g/l chitosan solution in acetic acid [1.5% (v/v)], glutaraldehyde ranging from 34 g to 56 g/l, and an activation time between 6 and 10 h. Under these conditions, above 80% of the enzyme was immobilized on the support. The behavior of the keratinase loading on the chitosan beads surface was well described using the Langmuir model. The maximum capacity of the support ($q_m$) and dissociation constant ($K_d$) were estimated as 58.8 U/g and 0.245 U/ml, respectively. The thermal stability of the immobilized enzyme was also improved around 2-fold, when compared with that of the free enzyme, after 30 min at $65^{\circ}C$. In addition, the activity of the immobilized enzyme remained at 63.4% after it was reused five times. Thus, the immobilized enzyme exhibited an improved thermal stability and remained active after several uses.

• 한국비블리아학회지
• /
• 제29권3호
• /
• pp.13-40
• /
• 2018

본 연구에서는 한국의 지역대표도서관과 비슷한 위계에서 유사한 기능을 수행하는 일본의 도도부현립(都道府縣立)도서관의 상호협력체계 분석을 통하여, 국내의 지역대표도서관 건립의 기초 자료로 활용하고자 한다. 연구를 위해 일본 문부과학성이 선정한 도도부현립(都道府縣立)도서관 우수 선진 사례 도서관 8개 관을 방문하여 상호협력 체계와 상호협력서비스 현황을 분석하고, 그 중 5개 관을 선정하여 도면분석을 통한 공간구성 분석을 진행하였다. 그 결과 도도부현립(都道府縣立)도서관은 일본의 지역대표도서관으로서 역할과 기능을 정확하게 인지하고 전국구 단위에서 국립 국회도서관뿐만 아니라 인근의 지역대표도서관과도 상호협력을 시행하며 범국가적인 상호협력체계를 형성하고 있는 것을 확인하였다. 또 공간구성에서는 도서관의 역할에 따라 사무영역과 지원영역의 업무관리부문의 구성비가 가장 높게 나타나는 공간 구성비를 나타내고 있었다.

• Journal of Microbiology and Biotechnology
• /
• 제18권5호
• /
• pp.933-941
• /
• 2008

An extracellular $\beta$-glucosidase produced by Monascus purpureus NRRL1992 in submerged cultivation was purified by acetone precipitation, gel filtration, and hydrophobic interaction chromatography, resulting in a purification factor of 92-fold. A $2^2$ central-composite design (CCD) was performed to find the best temperature and pH conditions for enzyme activity. Maximum activity was observed in a wide range of temperature and pH values, with optimal conditions set at $50^{\circ}C$ and pH 5.5. The $\beta$-glucosidase showed moderate thermostability, was inhibited by $HgCl_2$, $K_2Cr_O_4$, and $K_2Cr_2O_7$, whereas other reagents including $\beta$-mercaptoethanol, SDS, and EDTA showed no effect. Activity was slightly stimulated by low concentrations of ethanol and methanol. Hydrolysis of p-nitrophenyl-$\beta$-D-glucopyranoside (pNPG), cellobiose, salicin, n-octyl-$\beta$-D-glucopyranoside, and maltose indicates that the $\beta$-glucosidase has broad substrate specificity. Apparently, glucosyl residues were removed from the nonreducing end of p-nitrophenyl-$\beta$-D-cellobiose. $\beta$-Glucosidase affinity and hydrolytic efficiency were higher for pNPG, followed by maltose and cellobiose. Glucose and cellobiose competitively inhibited pNPG hydrolysis.

• 한국동물위생학회지
• /
• 제43권2호
• /
• pp.99-105
• /
• 2020

This case describes outbreaks of acute aspergillosis in a red-crowned crane. A six-month-old, male, crane had showed clinical signs (i.e. anorexia, performance loss, ruffled feathers and drooped wings and open mouth breathing, etc.) before death. In necropsy examination, spherical to oval nodules disseminated from the respiratory tract to other organs. Those nodules were formed predominantly in air sacs, lung, peritoneum, serosa of esophagus and trachea. The nodules varied in size from 1 mm to over 1cm and the color was white to yellow. Microscopically, most of lung architecture were replaced by multiple foci which were characterized by well demarcated eosinophilic and karyorrhetic debris and surrounded by numerous Inflammatory cell. Most within necrotic center of the nodules, large numbers of fungal hyphae were present. Microbiology result indicated fungal growths on sabroud dextrose agar and bacterial growths on blood agar. Bacteria identified as E. rhusiopathiae using MALDI-TOF (microflex, BRUKER, USA) and fungi identified as A. fumigatus, A. terreus by sequencing the ITS1 and ITS4 regions. To confirm the route of infection, we checked the existence of the same pathogens in cohabitant (i.e. mother crane). The young age and weakened immunity (i.e. bacterial infection, etc.) causes fatal aspergillosis in birds.

• 한국식품조리과학회지
• /
• 제19권5호
• /
• pp.562-570
• /
• 2003

The awareness of ritual foods in general rituals were surveyed and compared in the Gyeonggi and Gyungsangbuk-do areas in order to identify the characteristics of Korean rituals and establish desirable ritual foods. As materials for "Jeon" (fried pan cakes) in rituals, fish fillets, meat and vegetables were largely used in the Gyeonggi region. In the Gyeongbuk region, all three of these ingredients were the most highly used for "Jeon" also. In the case of rice cakes, "Songpyun" steamed on a layer of pine needles, "Jeolpyun" and "Sirudduk", with a red beans, were mostly used in Gyeonggi-do, while "Songpyun", "Jeolpyun" and "Ingelmi" were largely used in Gyeongsangbuk-do. As seasoned vegetables and herbs, fernbrakes, root of bell flowers, green bean sprouts and bean sprouts were largely used in Gyeonggi-do region, whereas, fernbrakes, bean sprouts, root of bell flowers and spinaches were mainly used in Gyeongbuk region. The use of fernbrakes was highest in both regions. With regard to the number of side dishes, 2∼3 kinds of seasoned herbs and 3∼4 kinds of fruits were mostly used, but with slightly higher numbers in the Gyeongsangbuk-do than the Gyeonggi-do region. With regard to liquor used for rituals, clear strained rice wine was used most in the Gyeonggi-do area, while more unrefined rice wine was used in the Gyeongbuk region. Meat was the most used ingredient in broth slices of dried meat and cod were highly used in the Gyeonggi region, but slices of dried squid were most widely used in the Gyeongbuk region. Most households in both regions tended not to use raw fish in the rituals, and as for the ingredients of Korean Kabobs, meat was the most widely used, then fish and finally vegetables were the most used ingredients. Beef soup was the most used, but more green vegetable soup was used in the Gyeongbuk than the Gyeonggi region. Sweet drink made from fermented rice (sikhe) was generally used in the rituals. It was the most widely used in the Chusok-Hangawi Ritual in the Gyeonggi region, while it was used in the New Year's Ritual in the Gyeongbuk region.

• 한국환경보건학회지
• /
• 제45권5호
• /
• pp.474-486
• /
• 2019

Objectives: This study was performed to investigate the emission characteristics of aldehydes emitted by the printing industry and to evaluate their impact on adjacent residential areas. Methods: Aldehydes and THC were measured from the front of the control device in the printing and coating process. Aldehydes and ketones were measured by workplace area, residential area, and background area to evaluate their impact. Results: The concentrations of formaldehyde (<0.047 ppm) and acetaldehyde (<0.068 ppm) in the printing and coating process were relatively low, and the methyl ethyl ketone used as the primary solvent was the main carbonyl compound in the printing process. The daily mean concentrations of formaldehyde and acetaldehyde were not significantly different by workplace area, residential area, or background area. However, the concentration of methyl ethyl ketone was high in the order of workplace area, residential area, and background area. Conclusions: The concentrations of formaldehyde and acetaldehyde in the adjacent residential areas are considered to be more influenced by secondary sources of photochemical reactions than by primary sources. Methyl ethyl ketone is considered to be highly influenced by the primary source, which is printing facilities.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2017년도 학술발표회
• /
• pp.494-494
• /
• 2017

국내의 호소는 대부분이 농업용수 공급을 목적으로 설치된 인공 호소이다. 최근 이상기후 및 유역 오염원 증가로 호소의 수질오염도가 증가 추이에 있으며, 특히 하절기 외기온도 상승과 함께 녹조 대량발생, 어류 집단폐사 등 수질오염사고가 야기되고 있다. 이러한 수질오염사고는 호소의 지역적 특성으로 인한 여러 가지 원인이 있겠지만 직접적인 원인으로는 호소의 산소 고갈에 기인된다. 본 연구에서는 농업용 호소의 수온, DO, EC 등에 대한 일주기 및 계절적 동적변화를 규명하여 호소의 수질개선대책수립에 활용하고자 하였다. 연구대상 지구는 경기도 시흥시에 소재하고 있는 M 농업용 저수지이며, 2015년 6월 4일부터 9월 22일까지 수온, DO, EC을 측정할 수 있는 연속 자동측정장치를 설치하였고, 측정지점은 저수지 수심을 상(바닥층에서 1.5m 상부), 중(바닥층에서 1.0m 상부), 하(바닥층에서 0.5m 상부)로 구분하여 각각 측정하였다. 호소의 수온은 일주기로 수온성층 형성과 대류혼합이 반복되었으며, 상 하층간의 수온 차이가 평균 $1.4^{\circ}C$, 최대 $5.0^{\circ}C$의 차이를 보였다. 또한 강우가 발생한 이후에는 일시적으로 저수지 상 하 층간 수온 차이가 거의 없는 것으로 나타났는데, 이는 강우에 의한 수체의 수직혼합으로 저수지 전체의 물질순환에 영향을 미친 것으로 판단된다. 호내 DO 농도는 일(day) 주기 동안 농도변화가 크게 나타났으며, 여름철 무강우 기간에는 저수지 바닥층의 평균 DO 농도가 약 0.5mg/L로 거의 무산소 상태가 지속되는 것으로 관측되었다. DO는 기온이 낮아지거나 강수가 발생할 때 대류에 의한 수직혼합에 의해 간헐적으로 회복되다가 다시 고갈되는 현상이 반복되는 것으로 나타났다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 춘계학술대회
• /
• pp.105-105
• /
• 2019

Dendropanax morbifera H.Lev is an evergreen tree that lives in subtropical climates. About 75 species of D. morbifera live in East Asia, but only one species live in Korea(Korean dendropanax). D. morbifera belonging to Aralicacae family 98% of D. morbifera are distributed in Jeollanam-do in the Korea and the grow wild in Gyeongsangnam-do and Jeju Island. The harvest time and usage of D. morbifera were recorded in traditional medicinal books. The roots and stems of D. morbifera had been used for traditional medicine to treat migraine, menstrual irregularity and skin disease. And D. morbifera leaves are contain flavonoids and polyacetylene compounds. In this study, we were investigated the physiological activity of D. morbifera by different areas collected at the same time, and compared to characteristics of plants. D. morbifera collected from Jeollanam-do (Goheung-gun), Gyeongsangnam-do (Namhae-gun) and Jeju Island, and dried at $50^{\circ}C$ for three days. We used dried D. morbifera powder for antioxidant tests. Each sample was extracted with hot water under the same conditions. The contents of total polyphenols and total flavonoids from D. morbifera were identified. Also, we performed to DPPH radical scavenging activity, ABTS cation radical scavenging activity and Superoxide anion scavenging activity efficacy for antioxidant activity determination. The contents of total polyphenols and total flavonoids in hot water extract of D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do were higher than Jeju. However, D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do showed no significant difference those content of total polyphenols and total flavonoids. And the antioxidant capacity was showed the similar patterns in antioxidant activity.