• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.130-135
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• 2000

The characteristics of two different modes of perfusion culture, intermittent and continuous bleedings, were investigated by culturing the hybridoma cells producing von Willebrand Factor (vWF) monoclonal antibody (McAb) in a 15 L bioreactor without clogging the filter. Both culture methods exhibited similar profiles of cell density and metabolite concentrations during the culture period at the cell concentration of around 1${\times}$107 cells/mL. When the perfusion rate was increased, the intermittrnt bleeding culture showed problems of ammonia accumulation and decrease of cell viability. The continuous bleeding culture in terms of nutrient consumption and metabolite production kinetics. But the analysis of specific oxygen consumption rate showed that the specific oxygen consumption rate of intermittent bleeding culture was similar to that of exponential growth phase. The continuous bleeding culture showed higher specific oxygen consumption rate of intermittent bleeding culture. finally we proved the possibility of long-term operation of continuous bleeding culture and produced approximately 40 g of vWF McAb in a 15L bioreactor after one-month operation.

• Journal of Sasang Constitutional Medicine
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• v.18 no.1
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• pp.107-117
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• 2006

1. Objectives There are many studies on the ordinary symptoms based on Sasang constitution. But there. are not quite satisfactory between the types of Sasang constitution. So This study is for learning the characteristics of ordinary symptoms depending on Soyangin Soeumin and Taeyangin Taeumin of the Sasang constitution. 2. Methods One thousand and two hundred twenty nine subjects were included in Bundang Oriental Hospital of Dongguk University. We classified them into the each type of Sasang Constitutional Medicine(SCM) by the well-trained SCM specialist, and assessed their ordinary features by the questionnaire. Binary logistic regression analysis was applied to evaluate the influence of ordinary features to the diagnosis of SCM. 3. Results (1) Compared to Taeyangin Taeumin, Soyangin Soeumin do not sweat a lot. Even if Soyangin Soeumifl do sweat, one does not feel refreshed. Soyangin Soeumin do not sweat while sleeping at night nor eating meals, but discharges cold sweat if one does not feel well. (2) Compared to Taeyangin Taeumin, Soyangin Soeumin go to stool less frequently and have diarrhea when one does not feel well. Soyangin Soeumin do not feel irritated even though one is not able to go to stool for one day. (3) Compared to Taeyangin Taeumin, Soyangin Soeumin do not have foams in urine. (4) Compared to Taeyangin Taeumin, Soyangin Soeumin tend to eat slow, do not eat a lot normally, do not have a great appetite, have problems for digestion, and especially have difficulties in digestion when one is stressed. 4. Conclusions We found that Taeyangin Taeumin and Soyangin Soeumin have characteristics of ordinary symptoms, but partly there are not in accoradance with ones what Lee ]e-Ma said in his book. So in future we hope clinical studies are required steadily.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.2
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• pp.100-106
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• 2014

The present study investigated variations in soil microbial communities by fatty acid methyl ester (FAME) and the chemical properties at 24 sites of upland soils in Gyeongnam Province. The electrical conductivity of the soil under potato cultivation was significantly higher than those of the red pepper and soybean soils (p < 0.05). The gram-negative bacteria community in potato soil was significantly lower than those in the garlic and soybean soils (p < 0.05). The communities of actinomycetes and arbuscular mycorrhizal fungi in the red pepper soil were significantly higher than those in the potato soil (p < 0.05). In addition, the cy17:0 to 16:$1{\omega}7c$ ratio was significantly lower in red pepper, soybean, and garlic soils compared with potato soil, indicating that microbial stress decreased. Consequently, differences in soil microbial community were highly associated with cultivated crop species, and this might be resulted from the difference in soil chemical properties.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.93-96
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• 2012

'Double Shine', a cultivar of Gypsophila paniculata was newly developed for the cut flowers by Jeollabuk-do Agricultural Research & Extension Services. It has morphological characteristics of the spreading plant type, double florets of many petals and two pistils, emarginate petal tip and cup shaped calyx. Also 'Double Shine' has several traits such as strong flower stalk, bright colored flower, narrow branching angle and more or less resistance to powdery mildew and pest injury. Furthermore, 'Double Shine' produced higher primary branches, and longer flower stalk length with internodal length and than did a check variety 'Bristol Fairy' during summer cultivation in subalpine areas. Blooming of 'Double Shine' was delayed by 16 days, its floret had broader width, compared to 'Bristol Fairy'. Moreover, 'Double Shine' produced normal flowers with two pistils and 8.5 stamens, with little production of malformed flowers in the high temperature condition.

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.838-843
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• 2017

Sepsis is a major health problem worldwide, with an extremely high rate of morbidity and mortality, partly due to delayed diagnosis during early disease. Currently, sepsis diagnosis requires bacterial culturing of blood samples over several days, whereas PCR-based molecular diagnosis methods are faster but lack sensitivity. The use of biosensors containing nucleic acid aptamers that bind targets with high affinity and specificity could accelerate sepsis diagnosis. Previously, we used the systematic evolution of ligands by exponential enrichment technique to develop the aptamers Antibac1 and Antibac2, targeting the ubiquitous bacterial peptidoglycan. Here, we show that these aptamers bind to four gram-positive and seven gram-negative bacterial sepsis agents with high binding efficiency. Thus, these aptamers could be used in combination as biological recognition elements in the development of biosensors that are an alternative to rapid bacteria detection, since they could provide culture and amplification-free tests for rapid clinical sepsis diagnosis.

• Journal of Environmental Health Sciences
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• v.44 no.3
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• pp.250-258
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• 2018

Objectives: The study examined indoor air quality during daytime and commute times in underground parking lots in five apartments located in northern Gyeonggi province. Methods: This study examined the temporal characteristics of $PM_{10}$, $CO_2$, CO, HCHO and VOCs in the indoor air quality of the underground parking lots in five apartments. Results: $PM_{10}$ concentration in daytime were ranged from 37.4 to $69.9{\mu}g/m^3$ which complied with the indoor air quality maintenance standard ($200{\mu}g/m^3$) and Gyeonggi province ordinance standard ($180{\mu}g/m^3$). However $PM_{10}$ concentrations in commuting time were in range of $447.3{\sim}944.0{\mu}g/m^3$ that exceeded for both criteria. The $CO_2$, CO and HCHO concentrations complied with the standard. The VOCs indicated high tendency in the order of toluene, xylene, ethylbenzene and benzene. This tendency was more apparent in commuting time than daytime. Conclusion: All indoor parking lots of five apartments were equipped with ventilators. However the most apartment residents did not operate blowers to save electricity and the lack of interest for indoor air quality. In order to improve the indoor air quality of underground parking lots in apartment, operating ventilation system during commuting time is recommended.

• Korean Journal of Environmental Agriculture
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• v.18 no.4
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• pp.339-343
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• 1999

This study was carried out to investigate the effects of treatments and adequate concentration of ethephon on coloring promotion of peel of sweet persimmon 'Nishimurawase' cultivar. High concentration(30 and 40㎎/l) of ethephon treatments increased the coloring of peel. but they were unfavorable to marketability due to the decrease of firmness, the increase of softening ratio, and the short of marketing critical period of fruits. Low concentration(20㎎/l) of ethephon treatment maintained higher marketability in terms of coloring of peel, firmness, softening ratio, and marketing critical period of fruit.

• Journal of Korean Society on Water Environment
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• v.28 no.4
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• pp.505-511
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• 2012

While the existing sewage treatment facilities are mainly being operated by biological processes, winter-time efficiency improvement and additional phosphorus treatment equipment using chemicals have been required to follow the effluent criteria of TP (0.2, 0.3 and 0.5 mg/L for the zone of I, II and III respectively) and $BOD_5$ (5.0 mg/L) which is intensified from 2012 in Republic of Korea. We made an investigation into actual condition of biological treatment process and calculated the optimal chemical input amount by jar test of supernatant of secondary sedimentation tank to evaluate the process improvement for the intensified criteria. Ejector BAF system for removing TP, $BOD_5$ of sewage effluent was suggested. The concentration of TP from biological process is 0.3-0.8 mg/L, and the input amount of optimal chemical coagulant was above Al/P ratio of 3(1.9 mg/L as Al) to meet the criteria of TP for secondary treatment effluent. From the results of this experiment, the best Al/P ratio for Ejector BAF system was about Al/P ratio of 1, and LV of BAF process for intensified criteria of $BOD_5$ and TP was below 1.97m/hr.

• The Journal of the Korea Contents Association
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• v.13 no.10
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• pp.46-53
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• 2013

An inspection of nuclear fuel rods should be performed at remoteness from risks of high level radioactivity, and accuracy is required. Currently, inspection of the nuclear fuel rods is operated to monitor the video that recording an original nuclear fuel rods at remoteness because of the risks of radioactivity. In this paper, it is an implementation of the system was carried out in the process according to the image processing inspection of the nuclear fuel rods. The nuclear fuel rods are configured to use a bundle of plurality, in the image processing technology to verify this, the edge detection method is useful. We suggest to DoG technique to add threshold for the nuclear fuel rod edge detections. This is the new technique that optimized DoG. It is to deal with DoG and threshold to dual process. In this way, after detecting an edge of the nuclear fuel rods, by running a nuclear fuel rod inspection algorithm to determine the status of nuclear fuel rods. We applied the system using the new algorithm, and confirmed an excellent characteristic. In this study, it is considered to be able to be carried out more easily and securely inspect of nuclear fuel rods.

• Journal of the Korean Society of Costume
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• v.10
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• pp.5-16
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• 1986

This dissertation is a study of the costume of the Yi dynasty by means of an investigation of the Ka-rae-do-gam-Eui-gue Do(嘉禮都監儀軌圖: a collection of paintings of the royal wedding ceremonies and processions issued by the royal court) and the Jung-ri Eui-gue Do(整理儀軌圖: a collection of a series of paintings showing the whole process of the royal courtesies and ceremonies on the occasion of the king's visit to Hyun-yoong Won in Hwa-sung in 1975年). The Yi dynasty period is roughly divided into two parts. The first period extends from 1392, in which the reign of the dynasty started, to 1600, when Imjin Waeran(the Japanese Invasion of Korea) ended; the second period lasts from 1600 to the last day of the dynasty in 1910. Of the "Eui-gue Do"(儀軌圖: paintings of the royal ceremonies) which were made in the first period of the dynasty, there is no extant example, the reference to which is found only in records. However, the examples of the "Eui-gue Do" belonging to the second period remain abundant in number, together with the detailed accounts about them. The followings are the conclusions deduced from this study, which tries to illuminate the pictorial characteristics as well as the traits of costume manifested in the above-mentioned two groups of paintings. Most of the costume seen in the Ka-rae-do-gam Eui-gue and the Jung-ri Eui-gue were not clad in accordance with the wearer's individual desire or taste, but in strict conformity with the norm and sense of order in the society based on the Cosfucian political and ethical principles.