• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.5
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• pp.419-430
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• 1987

The damage of plasmid DNA by lipid peroxidation and its inhibition were investigated through the model system of DNA and linoleic acid at $37^{\circ}C$. The degree of DNA damage increased in proportion to the increase of concentration and peroxidation of linoleic acid. DNA damage induced from linoleic acid peroxidation was greatly inhibited by the addition of active oxygen scavengers, especially, singlet of oxygen scavenge$(\alpha-tocopherol,\;cysteine)$ and superoxide anion scavenger(superoxide dismutase, ascorbic acid) in reaction system. These active oxygens, such as superoxide anion and hydrogen peroxide were rapidly generated in the early stage of peroxidation (POV below 100 mg/kg) and also scanvenged by the addition of superoxide dismutase and catalase, respectively. Hydroperoxide isolated from autoxidised linoleic acid showed DNA damage. Hydroperoxide induced-DNA damage was not inhibited by active oxygen scavengers. Lipid oxidation products, malonaldehyde and hexanal, also influenced on the DNA damage. Accordingly, it is speculated that DNA damage by lipid oxidation products is due to active oxygens such as singlet oxygen and superoxide anion formed in the early stage of peroxidation, direct action of hydroperoxide and formation of low molecular carbonyl compound-DNA complex. Furthermore, DNA damage induced by lipid peroxidation was remarkably inhibited by the addition of active oxygen scavengers and natural antioxidative fractions extracted from garlic and ginger. These antioxidative fractions also suppressed the generation of active orygens and linoleic acid oxidation. It is assumed that the inhibition of DNA damage by garlic and ginger extracts is due to the scavenging effect of active oxygens and the inhibition of hydroperoxide and oxidation products formation.

• Bulletin of Food Technology
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• v.23 no.2
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• pp.198-204
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• 2010

• Journal of the Korean Chemical Society
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• v.49 no.6
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• pp.546-553
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• 2005

• Journal of Sensor Science and Technology
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• v.21 no.5
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• pp.359-366
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• 2012

Nanopore DNA sequencing is an emerging and promising technique that can potentially realize the goal of a low-cost and high-throughput method for analyzing human genome. Especially, solid-state nanopores have relatively high mechanical stability, simple surface modification, and facile fabrication process without the need for labeling or amplification of PCR (polymerized chain reaction) in DNA sequencing. For these advantages of solid-sate nanopores, the use of solid-state nanopores has been extensively considered for developing a next generation DNA sequencing technology. Solid-state nanopore sequencing technique can determine and count charged molecules such as single-stranded DNA, double-stranded DNA, or RNA when they are driven to pass through a membrane nanopore between two electrolytes of cis-trans chambers with applied bias voltage by measuring the ionic current which varies due to the existence of the charged particles in the nanopore. Recently, many researchers have suggested that nanopore-based sensors can be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000.

• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.586-588
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• 2004

최근 DNA 관련 기술의 개발이 활발하게 이루어지고 있고, 그에 따라 DNA를 개인인식에 사용마고자 하는 시도가 실시간 이용가능성이 높은 DNA 칩 기술과 합쳐져 매우 중요한 이슈로 떠오르고 있다. DNA를 분석하기 위해서는 생물학적 실험이 필수적으로 따르게 되는데 이러한 실험 결과를 인식에 적용하기 위해서는 적절한 전처리가 필요하다. 본 논문에서는 여러 장점들로 인해 최근 DNA분석 기술로 주목받고 있는 모세관 전기영동법을 사용하여 DNA를 분석하고, 그 분석물을 개인인식을 위해 genotyping하는 과정에서 전처리가 요구되는 각 경우들에 대해 논하고 적절한 필터링 기법들을 제시한다.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.818-827
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• 2010

A full-length cDNA and genomic DNA of a $leucoanthocyanidin$ $dioxygenase$ ($DgLDOX$) gene was isolated from the petals of chrysanthemum 'Argus', and comparative features of the gene among three flower color mutants derived from a gamma-ray mutagenesis were characterized. The cDNA coding region of the gene was 1068 bp and was translated into 356 amino acids accordingly. The genomic DNA size was 1346 bp for 'Argus', while three mutants revealed ranges of 1363 to 1374 bp. A single intron between two coding exons for the $DgLDOX$ gene was found, of which size was 112 bp for 'Argus', but 128 or 137 bp for three flower color mutants, indicating that a genomic insertion in the intron occurred during the gamma-ray mutagenesis. DNA blot analysis revealed the $DgLDOX$ gene presenting as a single copy in the chrysanthemum genome. The $DgLDOX$ gene was expressed in both 'Argus' of light-pink color and two purple color mutants (AM1 and AM3) but had very weak expression in only white color mutant (AM2). The results demonstrated that variations in the flower color of the mutants might be associated with changes in the amino acid moieties in the coding exons or fragment insertions in the intron of the $DgLDOX$ gene, which potentially resulted in less expression of the gene in the white colored mutant.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1607-1615
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• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.145-148
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• 2004

As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.

• Electronics and Telecommunications Trends
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• v.35 no.1
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• pp.25-33
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• 2020

Just as it is possible to distinguish people by using physical features, such as fingerprints, irises, veins, and faces, and behavioral features, such as voice, gait, keyboard input pattern, and signatures, the an IoT device includes various features that cannot be replicated. For example, there are differences in the physical structure of the chip, differences in computation time of the devices or circuits, differences in residual data when the SDRAM is turned on and off, and minute differences in sensor sensing results. Because of these differences, Sensor data can be collected and analyzed, based on these differences, to identify features that can classify the sensors and define them as sensor-based device DNA technology. As Similar to the biometrics, such as human fingerprints and irises, can be authenticatedused for authentication, sensor-based device DNA can be used to authenticate sensors and generate cryptographic keys that can be used for security.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.381-385
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• 1999

Taq DNA polymerase from Thermus aquaticus has been shown to be very useful in a polymerase chain reaction. Taq DNA polymerase has a domain at the amino terminus (residues 1 to 290) that has 5'-3' exonuclease activity and a domain at the C-terminus that catalyzes the polymerase reaction. Taq DNA polymerase is classified into the Pol I family, which is represented by E. coli DNA polymerase I. The alignment of amino acid sequences for the 5'-3' exonuclease domains of the Pol I family DNA polymerases shows ten highly conserved carboxylic amino acids. Crystallographic studies suggested that six of the carboxylic amino acids are clustered within a 7 $\AA$ radius by chelating three metal ions in the active site. Those six carboxylic residues are mutagenized to alanines in order to better understand their function. All six carboxylic residues, Asp l8, Glu1l7, Asp1l9, Asp120, Asp142, and Aspl44, are crucial for catalysis of 5'-3' exonuclease.