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Construction of the Full-length cDNA Library and Selection of Diapause-Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs  

Hwnag, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Go, Hyun-Jeong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Goo, Tae-Won (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Choi, Kwang-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Seong, Su-Il (Department of Life Science, College of Natural Science, University of Suwon)
Kim, Sung-Kuk (Department of Life Science, College of Natural Science, Daejin University)
Lee, Sang-Mong (Department of Sericulture and Entomology, Miryang National University)
Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Kim, Nam-Soon (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kang, Seok-Woo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.9, no.1, 2004 , pp. 145-148 More about this Journal
Abstract
As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.
Keywords
cDNA array; Embryonic diapause; Bombyx mori; Differential expression;
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