• Korean Journal of Plant Taxonomy
• /
• v.36 no.1
• /
• pp.21-40
• /
• 2006

We examined nrDNA ITS sequences from 16 taxa of Polygonum sect. Persicaria(Polygonaceae) in Korea to infer relationships among the taxa within the section. A neighbor-joining tree obtained from the analysis of the ITS sequences suggest that the ITS region was useful inferring the phylogenetic relationships among the taxa. The neighbor-joining tree indicates that P.amphibium is clearly separated from the other Korean taxa. The tree also reveals the presence of five major groups in the Korean taxa of the section; 1) P. lapathifolium var. lapathifolium, 2) P. persicaria and P. viscoferum, 3) P. orientale and P. viscosum, 4) P. japonicum and 5) a group including the remaining taxa. these relationships depicted on the ITS tree are largely congruent with those inferred from morphological and anatomical characters.

• Parasites, Hosts and Diseases
• /
• v.57 no.1
• /
• pp.55-60
• /
• 2019

This study was undertaken to determine the complete mitochondrial DNA sequence and structure of the mitochondrial genome of Spirometra ranarum, and to compare it with those of S. erinaceieuropaei and S. decipiens. The aim of this study was to provide information of the species level taxonomy of Spirometra spp. using the mitochondrial genomes of 3 Spirometra tapeworms. The S. ranarum isolate originated from Myanmar. The mitochondrial genome sequence of S. ranarum was compared with that of S. erinaceieuropaei (GenBank no. KJ599680) and S. decipiens (GenBank no. KJ599679). The complete mtDNA sequence of S. ranarum comprised 13,644 bp. The S. ranarum mt genome contained 36 genes comprising 12 protein-coding genes, 22 tRNAs and 2 rRNAs. The mt genome lacked the atp8 gene, as found for other cestodes. All genes in the S. ranarum mitochondrial genome are transcribed in the same direction and arranged in the same relative position with respect to gene loci as found for S. erinaceieuropaei and S. decipiens mt genomes. The overall nucleotide sequence divergence of 12 protein-coding genes between S. ranarum and S. decipiens differed by 1.5%, and 100% sequence similarity was found in the cox2 and nad6 genes, while the DNA sequence divergence of the cox1, nad1, and nad4 genes of S. ranarum and S. decipiens was 2.2%, 2.1%, and 2.6%, respectively.

• Korean Journal of Plant Taxonomy
• /
• v.49 no.2
• /
• pp.127-139
• /
• 2019

The purported four hybrid origins of Asplenium in Korea were tested based on morphological, cytological and DNA sequence data. Asplenium castaneo-viride, A. ${\times}$ uiryeongse, A. ${\times}$ montanus, and A. ${\times}$ kitazawae share several morphological characteristics with the Asian walking fern A. ruprechtii and related taxa as parents and show a sympatric distribution with the putative parents, raising the possibility of hybrid origins: A. castaneo-viride (A. ruprechtii and A. incisum), A. ${\times}$ uiryeongse (A. ruprechtii and A. pekinense), A. ${\times}$ montanus (A. ruprechtii, A. trichomanes, and A. incisum), and A. ${\times}$ kitazawae (A. ruprechtii and A. sarelii). We investigated flow cytometry and chloroplast DNA sequence data (rbcL, rps4-trnS, and rps4-trnS intergenic spacer) to clarify the hybridization and origin of each hybrid. In the flow cytometry analyses, A. ruprechtii shows diploid (2x) only, whereas A. castaneo-viride (3x, 4x), A. ${\times}$ uiryeongse (3x), A. ${\times}$ montanus (3x, 4x), and A. ${\times}$ kitazawae (2x, 4x) exhibit polyploidy, suggesting hybrid events along speciation. The rbcL and rps4-trnS and rps4-trnS intergenic spacer data suggest that A. ruprechtii is one the maternal ancestors of all four hybrids. In addition, the rps4-trnS and rps4-trnS intergenic spacer data indicate that A. incisum is also the maternal ancestor of A. ${\times}$ kitazawae and A. ${\times}$ montanus, proposing multiple hybridization events for these two hybrids. In A. ${\times}$ montanus, morphological features such as the leaf forms and sympatric distributions of the species also support the multimaternal hypothesis, but the morphological features of A. ${\times}$ kitazawae must be examined with consideration of hybrid events. To clarify the complex hybrid evolutionary lineages of the four Asplenium hybrids, further research with taxon sampling and molecular markers should be conducted.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.11
• /
• pp.1448-1456
• /
• 2023

A Gram-positive, non-motile, and non-spore-forming lactic acid bacterium, designated as BK2^T, was isolated from kimchi, a Korean traditional fermented vegetable food, and the taxonomic characteristics of strain BK2^T, along with strain LMG 11983, were analyzed. Both strains optimally grew at 30℃, pH 7.0, and 1.0% NaCl. Cells of both strains were heterofermentative and facultatively anaerobic rods, demonstrating negative reactions for catalase and oxidase. Major fatty acids (>10%) identified in both strains were C_18:1 ω9c, C_16:0, and summed feature 7 (comprising C_19:1 ω6c and/or C_19:1 ω7c). The genomic DNA G+C contents of both strains were 44.7 mol%. The 16S rRNA gene sequence similarity (99.9%), average nucleotide identity (ANI; 99.9%), and digital DNA-DNA hybridization (dDDH; 99.7%) value between strains BK2^T and LMG 11983 indicated that they are different strains of the same species. Strain BK2^T was most closely related to Weissella confusa JCM 1093^T and Weissella cibaria LMG 17699^T, with 100% and 99.4% 16S rRNA gene sequence similarities, respectively. However, based on the ANI and dDDH values (92.3% and 48.1% with W. confusa, and 78.4% and 23.5% with W. cibaria), it was evident that strain BK2^T represents a distinct species separate from W. confusa and W. cibaria. Based on phylogenetic, phenotypic, and chemotaxonomic features, strains BK2^T and LMG 11983 represent a novel species of the genus Weissella, for which the name Weissella fermenti sp. nov. is proposed. The type of strain is BK2^T (=KACC 22833^T=JCM 35750^T).

• International Journal of Industrial Entomology and Biomaterials
• /
• v.33 no.2
• /
• pp.72-77
• /
• 2016

The genus Scutellathous Kishii, 1955 is a small group known from Korea, Japan, and Taiwan. We carried out a taxonomic study on the Korean species of Scutellathous for the first time. As the result, S. porrecticollis (Lewis, 1894) is revealed to be commonly distributing in Korea and Japan. Additionally, we found that the previous record of Athous jactatus (Lewis, 1894) from North Korea is misidentification of S. porrecticollis. We herein provide redescription of S. porrecticollis and the result of DNA barcoding of congeners belonging to Scutellathous.

• Mycobiology
• /
• v.42 no.3
• /
• pp.291-295
• /
• 2014

Mycoflora was assessed in the commercial meju from four well-separated geographic origins. A total of 112 fungal isolates were identified by phenotypic characteristics and molecular taxonomy using sequencing the internal transcribed spacer of the rDNA and revealed 19 species from 13 genera. Enzymatic characteristics of protease and amylase, and mycotoxin production were analyzed.

• Mycobiology
• /
• v.44 no.3
• /
• pp.131-136
• /
• 2016

Gymnopus is a cosmopolitan genus of agaric fungi and consists of ~300 species. In Korea, Gymnopus represents common saprobic mushrooms, and 12 species have been reported in Korea. Several Gymnopus specimens were collected in Korea between 2008 and 2015. To identify them exactly, phylogenetic analysis was performed by means of the internal transcribed spacer region of ribosomal-DNA sequences from the collected Gymnopus specimens. Among them, G. iocephalus, G. polygrammus, and G. subnudus have not been reported in Korea. A phylogenetic tree and images are provided.

• The Korean Journal of Mycology
• /
• v.45 no.4
• /
• pp.362-369
• /
• 2017

This study evaluated macrofungal specimens collected from the Gangwon, Jeonnam, and Chungnam provinces of Korea in 2015~2016. All specimens were identified at the species level using a combination of morphological characteristics and rDNA sequence data. Among them, eight macrofungal species, Coprinopsis romagnesiana, Daldinia loculata, Gymnopus biformis, Gymnopus montagnei, Gymnopus sepiiconicus, Hebeloma danicum, Pholiota limonella, and Russula brevipes, are new records for macromycota in Korea.

• The Korean Journal of Mycology
• /
• v.45 no.4
• /
• pp.304-318
• /
• 2017

While evaluating fungal diversity in fecal and freshwater samples in Korea, three fungal strains, CNUFC-GHD83-1, CNUFC-RD8126, and CNUFC-YR2-1, were isolated from specific habitats including grasshopper and rat feces, and freshwater samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS) and 28S rDNA, the isolates CNUFC-GHD83-1, CNUFC-RD8126, and CNUFC-YR2-1 were identified as Albifimbria terrestris, Cephaliophora tropica, and Mariannaea aquaticola, respectively. These species have not been previously described in Korea.

• Korean Journal of Plant Taxonomy
• /
• v.48 no.1
• /
• pp.1-8
• /
• 2018

Mankyua chejuense is the only member of the monotypic genus Mankyua (Ophioglossaceae) and is endemic to Jejudo Island, Korea. To determine the precise phylogenetic position of M. chejuense, two cpDNA regions of 42 accessions representing major members of lycophytes are obtained from GenBank and analyzed using three phylogenetic analyses (maximum parsimony, maximum likelihood, and Bayesian inference). In addition, the divergence time is estimated based on a relaxed molecular clock using four fossil calibration points. The phylogenetic position of Mankyua still appears to be uncertain, representing either the earliest diverged lineage within Ophioglossaceae or a sister to the clade containing Ophioglossum and Helminthostachys. The most recent common ancestor of Ophioglossaceae and its sister lineage, Psilotum, was estimated to be 256 Ma, while the earliest divergence of Mankyua was estimated to be 195 Ma in the early Jurassic.