• 한국환경생태학회지
• /
• 제28권5호
• /
• pp.523-530
• /
• 2014

2011년 6월 28일에 파주시 조리읍 장곡리 인근 도로에서 두부쪽이 손상되고, 해당연도에 태어난 맹금류 어린새를 발견하였고, 이를 DNA 바코드 기법으로 동정하였다. Mitochondrial DNA(mtDNA) cytochrome c oxidase I(COI) 유전자의 695bp 절편을 중합효소연쇄반응(polymerase chain reaction, PCR)으로 증폭하고 염기서열을 결정하였다. 결정된 염기서열을 BOLD systems과 NCBI의 BLAST에서 유사도 분석을 수행한 결과 총 5개체의 왕새매가 검색되었고, 염기서열의 동일성은 100%로 조사되었다. 또한, DNA 분자성판별 결과는 해당 개체가 암컷임을 나타내었다. 이러한 결과는 경기도 파주에서 1968년 이후 43년만에 왕새매의 번식이 확인된 중요한 정보로, 향후 광역야생동물구조센터는 야생동물의 사체 수거 시 인근 기탁등록보존기관과 연계하여 DNA시료를 확보하고 보다 정확한 종동정과 성판별 정보를 기록하는 등의 체계적인 관리시스템이 필요할 것으로 사료된다. 또한 이렇게 확보한 왕새매의 DNA 시료와 DNA 바코드 COI 유전자 서열은 유사종 연구의 참조표본(reference standard)로 이용될 수 있을 것이다.

• IMMUNE NETWORK
• /
• 제1권2호
• /
• pp.109-115
• /
• 2001

Background: The role of the interferon consensus sequence binding protein (ICSBP), a member of interferon regulatory factor family, in protecting against a vesicular stomatitis virus (VSV) infection has not been firmly elucidated. Thus, it was investigated utilizing the human promyelocytic leukemia HL-60 cells which do not express ICSBP. Methods: HL-60 cells were stably transfected with plasmid containing cDNA for either ICSBP or DNA binding domain (DBD) and tested for their VSV-susceptibilities. The susceptibility of each transfectant group to a VSV infection was determined by a plaque assay at 1 h, 24 h, and 48 h post-infection in the presence (500 IU/ml) or absence of interferon ${\alpha}$ ($IFN{\alpha}$). Results: In the absence of $IFN{\alpha}$, the three groups showed similar sensitivities to a VSV infection. However, when pre-treated with IFN, the viral titers in both the ICSBP and control clones steadily decreased over 48 h of incubation, indicating the existence of $IFN{\alpha}$-mediated protection against VSV infection. The $IFN{\alpha}$-treated ICSBP clones appeared to be more resistant to infection compared with the control clones, although the difference was not great. On the contrary, the viral titers in the $IFN{\alpha}$-treated DBD clones increased at 24 h then decreased by 48 h. Conclusion: The expression of truncated ICSBP (DBD) does not appear to underlie the impaired protection against a VSV infection in the DBD clones, since even the control clones lacking ICSBP were protected from a VSV infection. This suggests that ICSBP does not play a critical role in the $IFN{\alpha}$- mediated anti-VSV response of HL-60 cells, although it appears to confer some resistance to a VSV infection.

• 동의생리병리학회지
• /
• 제16권6호
• /
• pp.1117-1121
• /
• 2002

To test the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was examined using MTT, Beating rate and DNA synthesis assay in the presence of water extract of three kinds of Radix Rehmanniae. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. The results of these experiments were obtained as follows : Xanthine oxydase/hypoxanthine resulted in a decrease in viability, beating rate and DNA synthesis in cultured myocardial cells. Radix Rehmanniae Recens(生地黃, RRR) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in beating rate. Radix Rehmanniae Preparat(熟地黃, RRP) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in DNA synthesis. These results show that xanthine oxydase/hypoxanthine elicits toxic effects in cultured myocardial cells derived from neonatal rat, and suggest that water extract of three kinds of Radix Rehmanniae is very effective in the prevention of xanthine oxydase/hypoxanthine-induced cardiotoxicity.

• 미생물학회지
• /
• 제28권3호
• /
• pp.192-198
• /
• 1990

플라스미드 pKM101과 이의 돌연변이제 pSLA의 mutator 유전자를 subcloning하여 재조합 플라스미드 pJB200과 pJB210을 선별하였고, umuC36- uvrA6-(TK 610) 균주에 도입하여 UV와 MMS에 대해서 보호효과와 돌연변이율에 미치는 영향을 조사하였다. 재조합된 플라스마드는 UV와 MMS에 대해서 nonmutability인 umu- 균주에서 완전히 돌연변이율을 회복시켰고 보호효과를 크게 증가시켰다. 이 사실은 muc 유전자가 cloning된 재조합 플라스미드가 돌연변이원의 처리에 의해 효과적인 발현을 하며, muc 유전자 만으로도 pKM101의 기능을 나타낸다는 것을 확인하였고, pSLA의 muc 유전자가 그 효과에 었어서 높은 영향을 미쳤다. 또한 pKM101의 muc gene을 포함한 pJB210 은 recA - (JC2926) 균주에서 돌연변이 유발능에 영향을 미치지 못한 것은 muc 유전자가 recA 유전자에 의존함을 알 수 있었다.

• 환경생물
• /
• 제18권2호
• /
• pp.205-213
• /
• 2000

실험실에서 형질전환될 수 있는 세균들은 자연환경 조건에서도 형질전환 능력이 발달하는 것으로 알려져 있다. 따라서, 환경 내에서 형질전환 능력이 있는 세균의 존재는 확실한 것으로 여겨진다. DNA는 무기물에 부착된 상태에서는 핵산분해효소에 의한 분해로부터 보호되는 것으로 알려져 있다. 비록 DNA가 토양 속에 분산되어져 일정 비율로 가수분해되더라도, 수 주일 후에도 낮은 비율로 감지될 수 있다. 따라서 free DNA는 자연적 형질전환을 할 수 있을 만큼 충분히 지속될 수 있다. 실험실 조건에서는 세균의 형질전환이 여러 경우 보고되었지만, 자연상태에서 형질전환과 관련된 자료는 매우 적다. 생태학적으로 GMMs로부터 재조합 DNA가 토착 미생물에 전이될 수 있는 잠재력에 대한 문제가 주요 현안이 되었는데, 이는 전이된 DNA가 방출된 세균의 생태학적인 적응력을 변화시켜 생물학적 안전성의 문제를 야기할 수 있기 때문이다. 물론, 방출된 GMMs로부터 재조합 DNA가 토착 미생물에 전이되는 율은 아주 낮은 빈도로 일어나지만, 빈도가 낮다는 것은 그리 중요하지 않다. 왜냐하면, 비록 낮은 빈도로 전이되더라도 유리한 조건을 만나게 되면 전이된 유전자는 선택될 수 있기 때문이다. 이제까지 GMMs는 실험실이나 제한된 환경에서 주로 사용되었지만 앞으로는 개방된 자연 생태계에서 이루어질 전망이다. 그러므로 GMMs가 토착세균에 미치는 영향에 대해서도 연구되어야 하고 동시에 GMMs가 생태계에 방출될 경우 그에 따른 영향평가를 반드시 수행해야 한다.

• The Plant Pathology Journal
• /
• 제34권6호
• /
• pp.514-531
• /
• 2018

Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates.

• 산업과 과학
• /
• 제2권3호
• /
• pp.1-7
• /
• 2023

최근 방사능 노출에 대한 염려에 대한 이슈가 높아지고 있다. 토양, 물, 공기, 작물등에 영향을 주며 장기적으로 환경오염 및 식량오염이 발생하며 나아가 사회적인 혼란 및 경제적 타격을 초래할 것으로 여겨진다. 방사능 노출로 질병을 일으키기도 하지만, 질병진단을 위한 방법으로, X선촬영, CT, PET-CT등 핵의학 검사를 실시하고, 암치료 목적으로 방사선 동위원소에 노출시키기도 한다. 후쿠시마 방사능 폐기물 방류소식으로 물, 특히 식수에 포함되는 방사선에 대한 헝가리의 사례 연구 및 남극 대륙의 Larsemann Hills 지역 검사에서 세계 보건 기구에서 권장하는 음용수의 규정된 방사능 한계 내에 있었다. DNA손상, 세포 및 장기손상, 암에 관련된 내용을 중심으로 방사선 보호제를 살펴보고, 또한 복구물질중 ACE억제제, 항산화제, 천연물질등에 관하여 여러 문헌을 조사하였다. 일상에서 방사능에 노출되지만 안전할 수 있는 이유는 아마도 방사선보호물질, 방사능 피폭에 대한 복구 물질이 있을 것으로 여겨, 가능한 물질들을 찾아보고자 한다.

• Journal of Microbiology and Biotechnology
• /
• 제12권4호
• /
• pp.598-602
• /
• 2002

Damage to cells exposed to radiation is primarily attributed to direct effects on the structure of cellular DNA. Radiation-induced damage of pBluescript SK plasmid DNA and Escherichia coli $DH5\alpha$ were examined in the presence of various branched oligosaccharides, polysaccharides, and/or 8-MOP (8-methoxypsoralen). Branched oligosaccharides efficiently protected DNA and cells exposed to ultrasoft X-ray and UV irradiation. In the presence of 0.2% (w/v) branched oligosaccharides and polysaccharides, DNA can be protected from damage due to W and ultrasoft X-ray by a factor of 1.3-2.1 fo1d and 3.2-8.3 fold, respectively. The protective effect of cells exposed to UV or ultrasoft X-ray was also observed by branched oligosaccharides. The combination of MOP, a photoreagent, with carbohydrates increased the protective effects for DNA and cells, compared with that of a single use of MOP or carbohydrate alone.

• Parasites, Hosts and Diseases
• /
• 제55권5호
• /
• pp.505-512
• /
• 2017

Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant ${\alpha}-GalCer$. As results, TgCPC1 DNA vaccine with or without adjuvant ${\alpha}-GalCer$ showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and $IFN-{\gamma}$ in the spleen compared to controls (PBS, pEGFP-C1, and ${\alpha}-GalCer$). Upon challenge infection with tachyzoites of T. gondii (RH), $pCPC1/{\alpha}-GalCer$ immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and ${\alpha}-GalCer$). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.

• 대한한의학회지
• /
• 제23권3호
• /
• pp.145-163
• /
• 2002

Objectives: The purpose of this investigation was to evaluate the effects of Woohwangcheongsim-won and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods: E20 cortical cells were dissociated in neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Woohwangcheongsim-won was added to the culture media for 24 hrs or 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Woohwangcheongsim-won's effects for neuronal death protection were evaluated by LDH assay, propidium iodide stain and phospho-H2AX immunostain and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family, PKCα, ca1pain I. Results & Conclusions : 1. This study indicated that Woohwangcheongsim-won's effects for neuronal death protection in hypoxia were confirmed by LDH assay, propidium iodide stain and phospho-H2AX immunostain in culture method of Embryonic day 20(E20) cortical neuroblasts. 2. Woohwangcheongsim-won's mechanisms for neuronal death protection in hypoxia are to reduce the membrane damage fraction, to restrain DNA truncate, to restrain inflow of cytochrome c into cellularity caused by Bak diminution, to reduce the caspase cascade intiator caspase-8 and the effector caspase-3, to reduce the calpain I activity and to increase PKCand its activity in the membrane fraction. (J Korean Oriental Moo 2002;23(3):145～163)