• Parasites, Hosts and Diseases
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• 제55권1호
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• pp.81-84
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• 2017

Trypanosoma cruzi is the etiological agent of Chagas disease. Epimastigote forms of T. cruzi can be readily cultured in axenic conditions. Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents employed as vehicles for hydrophobic compounds. In order to produce a reference plot of solvent dependent growth inhibition for T. cruzi research, the growth of epimastigotes was analyzed in the presence of different concentrations of ethanol (0.1-4.0%) and DMSO (0.5-7.5%). The ability of the parasites to resume growth after removal of these solvents was also examined. As expected, both ethanol and DMSO produced a dose-dependent inhibition of cellular growth. Parasites could recover normal growth after 9 days in up to 2% ethanol or 5% DMSO. Since DMSO was better tolerated than ethanol, it is thus recommended to prefer DMSO over ethanol in the case of a similar solubility of a given compound.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제15권4호
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• pp.303-316
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• 1993

The auricular cartilage grafts have been widely used in replacement of the temporomandibular joint disk. Cartilage grafts itself have a low metabolism and high survival rate after grafting. In processing the grafting materials, it was important to preserve the properties of chondrocyte proper. We used 15% glycerol and 10% DMSO (Dimethyl Sulfoxide) solutions for cartilage fixation before deep freezing. We have performed the allogenic auricular cartilage graft in the temporomandibular joint of 20 rabbits which 10 specimen was treated with 15% glycerol and the other 10 specimen was treated with 10% DMSO respectively and examined in 1, 2, 4, 6 and 8 weeks after operation histopathologically. The result were : 1. Inflammatory cell infiltration around the grafted material appeared more glycerol groups than DMSO groups at 1 week, but each group has no differences after 2 weeks. 2. Degenerative changes of grafted auricular chondrocytes were more deveolped in glycerol group than DMSO groups till 4 weeks, but there were no differences between two groups after 6 weeks. 3. Fibrous union between grafted fragment and mandibular condyle was prominent in DMSO group. 4. Vascular proliferation of the grafted auricualr cartilage was more developed in DMSO groups than glycerol group in early stage. 5. Amount of the additional growth of grafted auricular cartilage was more existed in DMSO groups than glycerol group. 6. General survival rate after grafting was more prominent in DMSO group. In summary, allogenic auricular cartilage grafts treated with 15% glycerol and 10% DMSO solution have supported to survivalbility as a cryopreservative agents, especially DMSO groups have little inflammatory cell infiltration in early stages and degenerative changes and additional growth are more prominent than glycerol groups.

• 폴리머
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• 제31권6호
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• pp.532-537
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• 2007

본 연구에서는 poly(vinyl alcohol)(PVA)를 dimethyl sulfoxide(DMSO)에 녹여 이 용액의 유변학적 특성에 미치는 PVA의 분자량분포의 영향에 관하여 고찰하였다. 동일 평균분자량인 경우에도 수지 혼합(resin blending)에 의해 분자량분포가 넓어지면 점도와 손실탄성률이 더 낮아짐을 알 수 있었다. 이것은 동일 평균 분자량의 경우 분자량분포가 넓은 PVA/DMSO 용액계의 자유체적이 증가되기 때문이라고 생각된다. 분자량 분포가 저장탄성률에 미치는 영향은, 14 wt%용액의 경우에는, 1.3 rad/sec보다 낮은 진동수 영역에서는 동일 평균분자량의 경우 분자량분포가 넓은 PVA/DMSO 용액계가 탄성이 더 크고, 1.3 rad/sec보다 높은 진동수 영역에서는 분자량분포가 좁은 PVA/DMSO 용액계가 탄성이 더 크게 측정되었다. 14 wt% 용액의 경우에 Modified Cole-Cole plot에서의 기울기는 분자량분포가 넓어지면 진동수에 따른 상전이 현상이 더 뚜렷해졌다.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.345-351
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• 2013

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: $99.5{\pm}0.8%$, DMSO 7.5%: $99.5{\pm}0.7%$, and DMSO 10.0%: $99.6{\pm}0.6%$). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: $96.2{\pm}2.3%$, DMSO 7.5%: $95.3{\pm}3.6%$, 10.0%: $96.6{\pm}1.8%$) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm ($98.4%{\pm}0.5$) and DMSO concentration level of 5.0% ($97.8{\pm}0.1%$) were not significantly different. However, with 7.5% ($97.2{\pm}0.6%$) and 10.0% DMSO concentrations ($95.9{\pm}0.2%$) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; $66.8{\pm}1.8%$, HR: $82.0{\pm}12.9%$) and 2 years (FR; $78.5{\pm}14.8%$, HR: $79.3{\pm}0.6%$) cryopreserved sperm were lower than control (FR; 100%, HR: $91.1{\pm}3.6%$) and 2 days cryopreserved sperm (FR; $99.6{\pm}0.6%$, HR: $96.6{\pm}1.8%$). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.739-747
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• 2000

This experiment was conducted to evaluate the influence of dimethyl-sulfoxise (DMSO, 0, 5, 50, 100 and $500{\mu}M$) on the motility and acrosome reaction of the frozen-thawed spermatozoa from 3 different bulls (Bull A, Band C). Also we evaluated the developmental capacity of bovine embryos fertilized in a medium containing DMSO at various concentrations. DMSO had negligible effects on the sperm motility and acrosome reaction in all three bulls. However, the development rates from 2 to 16 cells stage on the 3rd day after insemination with 50, 100 and $500{\mu}M$ DMSO in Bull-B, and up to the blastocyst stage fertilized with 5, 50, 100 and $500{\mu}M$ in Bull-A were significantly higher (p<0.05) than those of control ($0{\mu}M$ DMSO) group from each bull. Furthermore, the rates of blastocysts per cleaved embryos of 5 to $500{\mu}M$ DMSO group in Bull-A and of 5 to $100{\mu}M$ DMSO in Bull-C were also significantly higher (p<0.05) than those for their $0{\mu}M$ groups, respectively. These results indicate that DMSO at micromol level used for in vitro fertilization might stimulate the development of embryos for some bulls.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.238-239
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• 2010

Dimethyl sulphoxide (DMSO) is one of the widely-used secondary dopants in order to enhance the conductivity of poly(3, 4-ethylenedioxy-thiophene):poly(styrene sulfonate) (PEDOT:PSS) film. In this work, we investigated the effect of DMSO doping in to PEDOT:PSS on the electrical performance of the bulk heterojunction photovoltaics consisting of poly(3-hexylthiophene-2, 5-diyl) and phenyl-C61-butyric acid methyl ester. Correlation between the power conversion efficiency and the mechanism of improving conductivity, surface morphology, and contact properties was examined. The PEDOT:PSS films, which contain different concentration of DMSO, have been prepared and annealed at different annealing temperatures. The mixture of DMSO and PEDOT:PSS was prepared with a ratio of 1%, 5%, 15%, 25%, 35%, 45%, 55% by volume of DMSO, respectively. The DMSO-contained PEDOT:PSS solutions were stirred for 1hr at $40^{\circ}C$, then spin-coated on the ultra-sonicated glass. The spin-coated films were baked for 10min at $65^{\circ}C$, $85^{\circ}C$, and $120^{\circ}C$ in air. In order to investigate the electrical performance, P3HT:PCBM blended film was deposited with thickness of 150nm on DMSO-doped PEDOT:PSS layer. After depositing 100nm of Al, the device was post-annealed for 30min at $120^{\circ}C$ in vacuum. The fabricated cells, in this study, have been characterized by using several techniques such as UV-Visible spectrum, 4-point probe, J-V characteristics, and atomic force microscopy (AFM). The power conversion efficiency (AM 1.5G conditions) was increased from 0.91% to 2.35% by tuning DMSO doping ratio and annealing temperature. It is believed that the improved power conversion efficiency of the photovoltaics is attributed to the increased conductivity, leading to increasing short-circuit current in DMSO-doped PEDOT:PSS layer.

• 한국수산과학회지
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• 제44권6호
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• pp.635-643
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• 2011

Agar was prepared from Gelidium amansii collected from Jeju Island, South Korea. This agar preparation has high gel strength and low sulfate content compared with G. amansii agar from Morocco. Accordingly, agarose was made from the Jeju agar through the consecutive refining processes of dimethyl sulfoxide (DMSO) extraction and ethylene diamine tetra acetic acid (EDTA) washing. The physicochemical properties of the resulting agarose were compared with those from agarose prepared using only DMSO extraction. Consecutive DMSO extraction and EDTA washing more strongly affected the physicochemical properties of the agarose (purified agarose) compared with the use of DMSO extraction alone. These properties were similar to those of commercial agarose used for electrophoresis. In DNA electrophoresis, the separation and movement speed of the purified agarose were similar to those of the commercial agarose. In a $^{13}C$ NMR analysis, the purified agarose exhibited the same carbon peak as the commercial agarose. When observed under scanning electron microscopy, the agar had an even and smooth surface without irregularities or pores, and the purified agarose had a wide surface area with a large number of pores; the commercial agarose had an irregular surface that would allow the solvent to easily permeate. These results illustrate that the physicochemical properties of agarose prepared from DMSO extraction and EDTA washing were more effective than those observed after DMSO extraction alone; thus, these processes used in succession will be useful in agarose industries.

• 대한화학회지
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• 제28권6호
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• pp.349-354
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• 1984

요오드화알칼리금속(NaI, KI, RbI, CsI)의 상대점도와 삼투 계수를 메탄올, 에탄올, dimethylsulfoxide (DMSO), and sulfolane (TMS) 용액에서 각각 측정하였다. 각 용매에서 요오드화 알칼리금속의 점도 A계수는 MeOH > EtOH > TMS > DMSO의 순위이고, 점도 B계수는 TMS > EtOH > DMSO > MeOH의 순위이다. 또 양성자성용매인 MeOH과 EtOH에서는 dB/dT의 순위가 NaI > KI > RbI > CsI이나, 비양성자성용매인 DMSO와 TMS에서는 반대순위이다. 한편 모든 용액에서 전해질의 삼투 계수는 Raoult 법칙의 양의 벗어남으로 인해 농도증가에 따라 삼투계수는 감소하였다. 각용매에서의 전해질의 삼투계수가 커지는 순위는 EtOH > DMSO > MeOH > TMS이다. 이러한 사실로 부터 요오드화알칼리금속은 양성자성용매와 비양성자성용매에서의 용매화현상이 매우 다르며, 이온쌍형성 및 용매의 구조파괴현상 등이 있음을 알았다.

• 한국식품과학회지
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• 제32권1호
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• pp.42-49
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• 2000

미세캡슐화된 고추올레오레진 capsicum을 사용하여 core material인 올레오레진을 파괴하지 않고, wall material를 제거함으로써 올레오레진 중의 capsaicinoids 함량을 측정하는 방법을 확립하였다. 미세캡슐을 용해할 수 있는 용매로서 dimethyl sulfoxide(DMSO)를 선발하였으며, DMSO에 혼합되는 용매로서 탄수화물을 침전시킬 수 있으면서 올레오레진에 대하여 높은 용해성을 가진 용매인 에탄올을 wall material 침전에 이용하였다. Wall material은 DMSO사용량에 대한 미세캡슐의 사용량의 비율이 $7.5{\sim}10%(w/v)$범위에서 최적이었으며 DMSO 사용량의 8배 이상의 에탄올을 첨가하였을 때 완벽한 추출이 되었다. 또한 동량의 에탄올을 사용하여 추출하였을 때 1회 추출 보다는 2회 반복추출이 완벽하게 capsaicinoids를 용해 할 수 있었다. 가열처리조건은 $55^{\circ}C$, 30분에서 DMSO가 wall materials를 용해시키기에 용이하였고 에탄올 첨가후 capsaicinoids를 추출하기 위한 vortexing time은 40㎐에서 3분 이상의 혼합에서 일정한 값을 보여주었고, 원심분리조건은 21000rpm의 회전속도에서 15분이상이 capsaicinoids함량에 최고치를 나타내었다. 확립된 방법은 고추올레오레진 미세캡슐의 제조 및 저장등의 capsaicinoids의 함량변화측정에 응용이 기대된다.

• 분석과학
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• 제7권4호
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• pp.505-515
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• 1994

DMSO(dimethylsuloxide-$d_6$) 용액 속에 존재하는 CN/CN 리간드의 hemin 착물과 CN/DMSO의 hemin 착물이 $^1H$ NMR로 기록되어지고 분석되어졌다. Hemin으로의 CN 착물화 과정은 온도에 따라 변화함을 NMR 스펙트럼이 보여 주며, 한 개의 CN 리간드에서 두 개의 CN 리간드착물로 바뀌는 과정의 열역학함수는 ${\Delta}H^{\circ}=736.6cal/mol$ 및 ${\Delta}S^{\circ}=16.4eu$인 흡열과정을 나타낸다. CN/DMSO의 hemin 착물은 Curie behavior로부터의 벗어남은 high-spin 성격의 존재를 나태내고, 이는 Fe-DMSO 결합이 순간적으로 깨짐을 의미하며, 이러한 CN/DMSO hemin 착물이 한 개의 axial ligand가 약한 heme 단백질의 전자 및 분자구조의 model complex로 작용할 수 있음을 보여 준다.