• Animal Bioscience
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• v.34 no.1
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• pp.36-47
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• 2021

Objective: This study determined the optimal ratio of whole plant corn silage (WPCS) to corn stover (stems+leaves) silage (CSS) (WPCS:CSS) to reach the greatest profit of dairy farmers and evaluated its consequences with corn available for other purposes, enteric methane production and milk nitrogen efficiency (MNE) at varying milk production levels. Methods: An optimization model was developed. Chemical composition, rumen undegradable protein and metabolizable energy (ME) of WPCS and CSS from 4 cultivars were determined to provide data for the model. Results: At production levels of 0, 10, 20, and 30 kg milk/cow/d, the WPCS:CSS to maximize the profit of dairy farmers was 16:84, 22:78, 44:56, and 88:12, respectively, and the land area needed to grow corn plants was 4.5, 31.4, 33.4, and 30.3 ha, respectively. The amount of corn available (ton DM/ha/yr) for other purposes saved from this land area decreased with higher producing cows. However, compared with high producing cows (30 kg/d milk), more low producing cows (10 kg/d milk) and more land area to grow corn and soybeans was needed to produce the same total amount of milk. Extra land is available to grow corn for a higher milk production, leading to more corn available for other purposes. Increasing ME content of CSS decreased the land area needed, increased the profit of dairy farms and provided more corn available for other purposes. At the optimal WPCS:CSS, MNE and enteric methane production was greater, but methane production per kg milk was lower, for high producing cows. Conclusion: The WPCS:CSS to maximize the profit for dairy farms increases with decreased milk production levels. At a fixed total amount of milk being produced, high producing cows increase corn available for other purposes. At the optimal WPCS:CSS, methane emission intensity is smaller and MNE is greater for high producing cows.

• Molecules and Cells
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• v.46 no.7
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• pp.441-450
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• 2023

β-Catenin (Ctnnb1) has been shown to play critical roles in the development and maintenance of epithelial cells, including the retinal pigment epithelium (RPE). Ctnnb1 is not only a component of intercellular junctions in the epithelium, it also functions as a transcriptional regulator in the Wnt signaling pathway. To identify which of its functional modalities is critically involved in mouse RPE development and maintenance, we varied Ctnnb1 gene content and activity in mouse RPE lineage cells and tested their impacts on mouse eye development. We found that a Ctnnb1 double mutant (Ctnnb1^dm), which exhibits impaired transcriptional activity, could not replace Ctnnb1 in the RPE, whereas Ctnnb1^Y654E, which has reduced affinity for the junctions, could do so. Expression of the constitutively active Ctnnb1^∆ex3 mutant also suppressed the development of RPE, instead facilitating a ciliary cell fate. However, the post-mitotic or mature RPE was insensitive to the loss, inactivation, or constitutive activation of Ctnnb1. Collectively, our results suggest that Ctnnb1 should be maintained within an optimal range to specify RPE through transcriptional regulation of Wnt target genes in the optic neuroepithelium.

• Animal Bioscience
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• v.37 no.6
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• pp.1031-1040
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• 2024

Objective: This study aimed to evaluate the effect of feeding milk replacer (MR) with varying levels of lactose and the increased supply of total solids (from 750 to 960 g/d) on performance, blood metabolites, and health of Holstein male calves during the preweaning period. Methods: Forty newborn Holstein calves (10 per treatment) were blocked according to birth weight and date of birth and distributed in a randomized block design to different liquid diets: Whole milk powder (WMP) diluted to 125 g/L solids; MR with 48% lactose (48L), diluted to 125 g/L solids; MR with 53% lactose (53L), diluted to 125 g/L solids; 53L MR corrected to 160 g/L solids (16TS) by the inclusion of a solid corrector. Calves were individually housed in wood hutches, fed 6 L/d of the liquid diet, and had free water and starter concentrate access. The study lasted 56 days. Results: Liquid diet intake was higher for calves fed 16TS than for other treatments. Calves fed 16TS presented higher protein and fat intake, followed by those fed WMP and the 48L or 53L MRs. Lactose intake was higher for 16TS-fed calves, followed by 53L, 48L, and WMP-fed calves. Starter and total dry matter intake did not differ among liquid diets. The average daily gain was higher for 16TS than 48L-fed calves, with the other treatments being intermediary. The lowest feed efficiency was observed for calves fed 48L. No effects on health were observed, as well as on selected blood metabolites, except for albumin concentration, which was higher for calves fed 16TS and WMP. Conclusion: Higher total solids content (160 g/L) in MR increases nutrient intake and consequently improves the performance of dairy calves. Feeding MRs with levels of lactose up to 53% of the DM had no deleterious effect on the performance or health of the calves.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.225-230
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• 2004

Jeju native sorghums were grown with six different planting densities ($5{\times}5cm,\;10{\times}10cm,\;15{\times}15cm,\;20{\times}20cm,\;25{\times}25cm\;and\;30{\times}30cm$) from April 3 to September 6 in 2000 in Jeju island to determine the influence to growth, yield and feed value by the planting densities. Days to heading were delayed from 66 days to 69 days as increasing of plant density. Plant height was 195.1cm at $30{\times}30cm$ plot, whereas that was 216.5cm at $10{\times}10cm$ plot, indicating that the plant was higher by the decrease of the planting density. As plant density increased, the number of leaves and steam diameter were decreased. Fresh forage yield increased from 25.4 to 61.3 MT/ha, dry matter (DM) yield from 5.8 to 16.5 MT/ha, crude protein (CP) yield from 0.4 to 1.5 MT/ha and total digestible nutrients (TDN) yield from 2.7 to 8.8 MT/ha as planting density from $30{\times}30cm\;to\;10{\times}10cm$ plot. Crude protein content increased from 7.0 to $9.0\%$, ether extract content from 1.7 to $4.4\%$, nitrogen free extract content from 36.9 to $38.4\%$ and TDN content from 46.1 to $55.5\%$ as planting density was decreased. whereas crude fiber content decreased from 43.9 to $37.1\%$ and crude ash content from 8.4 to $6.8\%$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.4
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• pp.254-261
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• 2014

This study was to assess the effect of sowing mixed legume forage and applying cattle manure on the productivity and feed value of whole-crop barley (WCB) as a representative of winter crops and to estimate feeding capacity of Korean native cattle (Hanwoo) per unit area by defining optimal application level of cattle manure for more fertile soil and manure recycling for the purpose of reduced environmental pollution and efficient production of organic forage contributable to production of organic animal products. Sowing mixed legume forage significantly (p<0.05) increased yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) for WCB compared to WCB alone sowing, but there was no difference between mixed sowing treatments. The yields of DM, CP and TDN were significantly (p<0.05) highest in 100~150 kg N/hg. The CP and acid detergent fiber (ADF) contents were highest in mixed sowing with hairy vetch, followed by sowing mixed forage pea and WCB alone (p<0.05). There were no differences in CP and neutral detergent fiber (NDF) contents among cattle manure application levels. As nitrogen application level increased, ADF content increased (p<0.05), but TDN content decreased (p<0.05). Feeding capacity of organic Hanwoo (head/ha) weighing 450 kg of body weight with 400 g of daily gain significantly (p<0.05) increased due to mixed sowing with legume forage, and with increasing application levels of fermented cattle manure, feeding capacity significantly (p<0.05) increased. The results indicate that mixed-sowing of WCB and legume forage not only cut nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity, and feeding capacity. This would be contributable to diversity of forage resources as well as production of organic animal product by creating low cost and high quality of forage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.2
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• pp.169-178
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• 2010

The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 mg/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate ($C_2$) proportion, however, was greater (p<0.001~p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest $C_2$ proportion was observed from SGS. The butyrate proportion was lowest (p<0.007~p<0.027) in the cattle fed BS at 1~6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

• Korean Journal of Organic Agriculture
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• v.23 no.2
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• pp.359-372
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• 2015

This study was to assess the effects of sowing mixed legume forage and applying fermented cattle manure on the productivity and feed value of whole crop wheat (WCW) as a representative of winter crops and also to estimate feeding capacity of Korean native cattle (Hanwoo) per unit area by defining optimal application levels of cattle manure for more fertile soil and manure recycling for the purpose of reduced environmental pollution and efficient production of organic forage contributable to production of organic animal products. Sowing mixed legume forage significantly (p<0.05) increased yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) for WCW compared to WCW alone sowing, but there were no differences between mixed sowing treatments. The yields of DM, CP and TDN were significantly (p<0.05) higher in 100~150 kg N/ha. The CP and acid detergent fiber (ADF) contents were highest in mixed sowing with hairy vetch, followed by sowing mixed forage pea and WCW alone (p<0.05). There were no differences in CP among cattle manure application levels. As nitrogen application level increased, ADF content increased (p<0.05), but TDN content decreased (p<0.05). Feeding capacity of organic Hanwoo (head/ha) weighing 450 kg of body weight with 400 g of daily gain significantly (p<0.05) increased due to mixed sowing with legume forage, and with increasing application levels of fermented cattle manure, feeding capacity significantly (p<0.05) increased. The results indicated that mixed-sowing of WCW and legume forage not only reduced nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity, and feeding capacity. This would be contributable to diversity of forage resources as well as production of organic animal product by creating low cost and high quality of forage.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.525-537
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• 2016

This study was aimed to grow organic forage as a prime requirement for organic Hanwoo cattle production over a period of 3 years (2012~2015) in Gyeongbuk region, Korea through mixed cropping of winter fodder barley with legume and application of fermented cattle manure. The effect of barley-legume mixed cropping and application of different levels of fermented cattle manure was studied on forage productivity of whole-crop barley (WCB) and evaluation of feeding capacity of Korean native cattle (Hanwoo) per unit area. The collected data was analyzed using SAS 9.3 software. Barley-legume mixed cropping increased (P<0.05) yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) as compared to those of sole WCB as monoculture. Similarly, acid detergent fiber (ADF) contents and relative feed value (RFV) were also higher (P<0.05) in barley-legume mixed sowing forage compared with those of sole WCB as monoculture. In case of manure application, yields of DM, CP and TDN were also found (P<0.05) highest in 100~150 kg N/ha. However, varying cattle manure levels did not influence (P>0.05) CP and neutral detergent fiber (NDF) contents. In response to increasing nitrogen application level, ADF content increased (P<0.05) but TDN content and RFV decreased (P<0.05). The Barley-legume mixed sowing and increasing application levels of fermented cattle manure also significantly enhanced (P<0.05) feeding capacity of organic Hanwoo (head/ha) having 450 kg body weight with 400 g of daily gain. Findings of present study indicated that barley-legume biculture not only cut nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity of forage as compared to WCB monoculture. This would be contribution to the diversity of forage resources as well as production of organic animal product by creating low cost and high quality organic forage.

• Journal of the Korean Wood Science and Technology
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• v.43 no.6
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• pp.851-860
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• 2015

The objective of this study was to manufacture the wood based roughage using lumber from thinning of oak and pitch pine (Pinus rigida). And the study also aimed to investigate a feed value evaluation of wood based roughages. To investigate the optimization condition of steam-digestion treatment for roughage, the wood chips of oak and pitch pine were steam-digestion treated at $160^{\circ}C$ under pressure 6 atm depending on treatment times (60 min, 90 min and 120 min) followed by the content of essential oils analyzed. The essential oil content of steam-digestion treated roughages for 90 min and 120 min were under 0.1 mL/kg. The evaluation of feed value was carried out from steam-digestion treated roughages for 90 min through feed chemical composition analysis, NRC (National research Council) modeling, ruminal degradability analysis and relative economic value analysis. The feed chemical compositions including DM (dry mater), CP (crude protein), EE (ether extract), NDF (neutral detergent fiber), ADF (acid detergent fiber), ADL (acid detergent lignin), NFC (nonfiber carbohydrate) in oak roughage were 95.4, 1.36, 3.11, 90.05, 83.85, 17.33, 6.50%, respectively, and in pitch pine roughage were 94.37, 1.33, 5.48, 87.89, 86.88, 30.56, 6.32%, respectively. Both roughages showed low level of protein and very high level of NDF. The TDN (total digestible nutrient) levels using NRC (2001) model in oak and pitch pine roughages were 40.55, 31.22%, respectively. The ruminal in situ dry matter degradability was higher in oak roughage (23.84%) than in pitch pine roughage (10.02%). The economic values of oak and pitch pine rough-ages were 235, and 210 \, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.49-58
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• 2012

This study was conducted to investigate the feeding fermented agro-industrial by-products on estimating the productivity and nutrient utilization of fattening black goats. Four experimental groups were set up with each of 6 animals for a 30 days feeding trial on growth performance and 12 black goats on nutrient digestibility were allocated to 4 groups of 3 animals each by latin-square design, control (offered concentrate and rice straw) and 3 treatment groups (offered fermentation feed and rice straw). Average daily gain (ADG) showed a significant high in control group, T3 was lower than other groups (p<0.05). DM and OM intake (kg/head) were 718.8, 680.9 g and 634.2~699.2 g, 602.8~660.4 g for control and treatment groups, respectively. As increasing of rice bran content was decreased feed intake. Digestibility of DM and OM for control group were significantly higher than treatment groups (p<0.05), and T3 treatment group with 30% rice bran was the lowest among treatment groups. Nitrogen retention was significantly higher in control and T1 groups. Thus, these results showed that productivity and nutrient utilization were similar between fermentation feeds with agricultural by-products and commercial feeds. However, this study was suggested that it must contain less than 5.0% crude fat because of feed efficient was decreased by increasing of rice bran content.