• Proceedings of the SCSK Conference
• /
• 2003.09a
• /
• pp.225-231
• /
• 2003

To search and development a new material with superior melanogenesis inhibitory activity, the bioactivities (obs. pl$_{50}$) of alkyl-3,4-dihydroxybenzoyl esters and N-alkyl-3,4-dihydroxybenz-oyl amides as substrate molecules were measured in mouse melanoma cells. And also, we have studied that 3-D QSARs (3 dimensional Quantitative Structure-Activity Relationships) between molecular interaction field of substrates and the bioactivities were analyzed using CoMFA (Comparative Molecular Field Analyses) method. When cross-validation value (q$^2$) is 0.68 at 3 components, the Pearson correlation coefficient ($r^2$) is 0.900. From the basis on the findings, the model was appeared by the contour map such as steric field and electrostatic field relationships between quantitative structure and the bioactivity of the various substrate derivatives. Measured bioactivities (obs. pl$_{50}$) of unknown compounds are very similar to predicted activity (pred. pl$_{50}$) according to the CoMFA model. As the results of prediction, we could conclude that the bioactivities were increased by creation of R$_1$ substitution of 5,5-dime-thylhexoxy, 6,6-dimethylheptyl, 1-amino-6,6-dimethylheptyl group etc and R$_2$ substitution of hydroxy, methyl, methoxy group etc.p etc.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.27 no.3
• /
• pp.537-542
• /
• 1998

The present study was performed to elucidate desmutagenic principles from Ecklonia stolonifera extracts against 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine(PhIP) and 2-amino-3,8-dime-thylimidazo[4,5-f]duinoxaline(MeIQx) with Salmonella/mammalian-microsome mutagenicity test. Alginate, phenols, chlorophyll and carotenoids from Ecklonia stolonifera were extracted and their desmutagenicities were assayed. Alginate hydroysates showed desmutagenic activities against PhIP and MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about 90% per 0.5mg against PhIP and MeIQx. Chlorophyllin among chlorophyll derivatives exhibited remarkable desmutagenic activities of 92.9% and 82.7% at 20uM against PhIP and MeIQx, respectively. Carotenoids, such as lutein and $\alpha$-cryptoxanthin isolated from Ecklonia stolonifera exerted also high desmutagenic activity. Major desmutagenic substances from Ecklonia stolonifera are considered to be chlorophyllin, phenols, lutein, $\alpha$-cryptoxanthin and low molecular alginates.

• Journal of the Korean Institute of Telematics and Electronics A
• /
• v.32A no.12
• /
• pp.184-197
• /
• 1995

In this paper, high speed IC package-to-package interconnections are modeled as lossless multiconductor transmission lines operating in the TEM mode. And, three mathematical algorithms for computing electrical parameters of the lossless multiconductor transmission lines are described. A semi-analytic Green's function method is used in computing per unit length capacitance and inductance matrices, a matrix square root algorithm based on the QR algorithm is used in computing a characteristic impedance matrix, and a matrix algorithm based on the theory of M-matrix is used in computing a diagonally matched load impedance matrix. These algorithms are implemented in a computer program DIME (DIagonally Matched Load Impedance Extractor) which computes electrical parameters of the lossless multiconductor transmission lines. Also, to illustrate the concept of design database for high speed IC package-to-package interconnection, a database for the multi conductor strip transmission lines system is constructed. This database is constructed with a sufficiently small number of nodes using the multi-dimensional cubic spline interpolation algorithm. The maximum interpolation error for diagonally matched load impedance matrix extraction from the database is 1.3 %.

• Journal of the Korean Society of Costume
• /
• v.42
• /
• pp.223-230
• /
• 1999

Dressmaking which is defined as the process of changing two-dimensional cloth into three-dime-nsional clothes needs a lot of joining parts. Particularly sleeve needs a more curved surface than other joining parts owing to shape of the top of arms. Easing is used to control the fullness along the seam-line to create the fullness curved shape of the sleeves and it plays an important role in increasing three-dimensional constru-ction power. unfortunately sufficient data regarding effective easing conditions to increase three-dimensional construction power of sleeve are not available. Therefore the author tried to investigate the effective easing conditions to increase three-dimensional construction power of sleeve by cap height. Easing construction power of sleeve by cap height. Easing contraction made by different angles on the sleeve cap curve line previous work were examined and the effective easing conditions according to the variation of angles were determined. This study contains the following objectives. Firstly to obtain the easing contraction ratio in sleeve according to the above easing conditions. Secondly to investigate the three-dimensional construction power or sleeve by the factors of easing contraction. Thirdly to determine for each sleeve the effective easing conditions on the basis of the calcuative easing contraction ratio in the sleeve patterns by cap heights.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.4
• /
• pp.698-703
• /
• 1994

In spore rec-assay using B. subtillus H17(rec) and M 45(rec) , the ethanol extract of buckwheat leaves showed antimutagenicity in condition of low concentrations, but its did comutagenicity in condition of high concentrations. In Ames test, the ethanol extract of buckwheat leaves reduced the mtabenicity of N-methyl-N' -nitro-N-nitrosoguaidine (MNNG), benzo (a) pyrene(B($\alpha$)P), 2-amino-fluorene(2AF), and 3-amino -1, 4-dime-thyl-5-H-pyrido(4, 3-b) indol(Trp-P-1) in Salmonella typhimurium TA98 and TA100. The ethanol extract was fractionated by hexane, ethylacetate, butanol and water. Among Them hexane fraction showed the highest inhibition rate on the mutagenicity of B($\alpha$)P, and so did chloroform fraction on the mutagenicity of MNNG in S. typhimurium Ta98 and TA100. To elucidate the antimutagenic mechanism of the ethanol extract, it was mixed and co-incubated with various metagens, S9 mix, and the bacteria with different experimental orders and different reaction times. The ethanol extract did not affect reversion rate of pre-mutated. S.typhimurium. However, when the ethanol extract was added to the mutagens before their interaction with S.typhimurium , it reduced the mutation rate to 152$\pm$12-273$\pm$18 colonies/plates in case of MNNG, and 135$\pm$13-195$\pm$10 colonies/ plates in case of B($\alpha$)P), showing strong desmutagenic activity.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.1
• /
• pp.50-56
• /
• 2002

The $\beta$-galactosidase was purified from the internal organs of edible snail by fractionation with ammonium sulfate, ion exchange chromatography on DEAE-Sephadex, Mono Q HR 5/5 and gel filtration on Sephacryl S-200. Superose 12 HR 10/30 chromatography. The specific activity of the purified $\beta$-galactosidase was 18.8 units/mg protein with 31.3 purification fold from crude extract. The $\beta$-galactosidase had native molecular weight of 144,000 dalton and was composed of two subunits of 72,000 dalton. The isoelectric point of the enzyme was determined 4.1. This enzyme was the most active at pH 3.0 and 6$0^{\circ}C$, and was stable in the pH range 2.0~8.0 and below 5$0^{\circ}C$. The enzyme was inhibited by metal ions and sugars such as fructose, glucose, galactose, maltose and xylose.

• BMB Reports
• /
• v.31 no.3
• /
• pp.274-280
• /
• 1998

An inositol monophosphatase (IMPase) was purified to homogeneity from rat duodenal mucosa for the first time and its enzymatic properties were investigated. Rat duodenal mucosa peculiarly exhibited the highest IMPase activity among various rat tissues examined. By means of ammonium sulfate precipitation, followed by Q-Sepharose, polylysine agarose, reactive-red agarose column chromatography, Uno-Q FPLC, and Bio-Silect FPLC, duodenal IMPase was purified 223-fold to a specific activity of 13.6 U/mg protein. The molecular mass of the native enzyme was estimated to be 48,000 Da on gel filtration. The subunit molecular mass was determined by SDS-PAGE to be 24,000 Da. These results indicate that duodenal IMPase is a dime ric protein made up of identical subunits. Rat duodenal IMPase has distinct properties from brain IMPase. It has a broad spectrum of substrate specificity and is insensitive to $Li^+$. Duodenal IMPase does not absolutely require $Mg^{2+}$ for its catalytic activity. Furthermore, duodenal IMPase is less stable to heat than brain enzyme. It is suggested that the rat duodenal mucosa needs a large amount of IMPase whose properties are quite different from that of the brain enzyme.

• Membrane Journal
• /
• v.19 no.1
• /
• pp.54-62
• /
• 2009

Pervaporation membrane for butanol separation was prepared by hybrid process. Plasma treatment of commercial poly(dimethylsiloxane) (PDMS) membrane was attempted and combination of plasma treatment and PDMS solution coating on polysulfone, poly(ether imide) supports were also performed. Plasma treatment of PDMS membrane with hexane and silane group compounds was performed to increase the hydrophobicity of the surface, which enhanced the separation factor upto 12.5 at the expense of flux decrease down to $1.15kg/m^2{\cdot}hr$. Contact angle and relative sorption ratio were also related with hydrophobicity of the memrbane. Increase of PDMS prepolymer composition resulted in dense structure of coating layer with better separation factor. Effects of sequence of PDMS coating vs. plasma treatment were examined. It was found that plasma treatment with butanol and n-hexane plasma followed by PDMS coating showed better performance and vice versa for plasma treatment with hexamethyldisilane and hexamethyldisilazane.

• Korean Journal of Child Studies
• /
• v.3
• /
• pp.49-62
• /
• 1982

This is a study to analyze rural area women's perception and practice of prenatal care, which is the fundamental of the education of children. Further, it was aimed to provide basic data for ideal childbirth, upbringing and household management process. For that object, questionnaires were distributed to women living in Yongdong region to judge the level of general understanding on prenatal care. Research was done from three different aspects; dietary life, emotion control and health management. Percentages were drawn out to see the general tendency. The following results were discovered from data analysis: 1. Most subjects of the survey knew well about prenatal care. They had acquired that knowledge from their parents. Most thought that prenatal care is necessary. They answered that they think prenatal care has more or less effect on the fetus. The first dime they thought about prenatal care was after they found that they were pregnant. 2. The subjects knew well about dietary needs for pregnant women carried out much of what they knew. Intake of sufficiently nutritious food is somewhat difficult, but foods that are regarded as bad were avoided thoroughly. It appears that most women have much concern about what they eat during pregnancy. 3. Most of the subjects understood that the emotional stability is necessary but it wasn't put into practice so well as in dietary life. Especially, attitudes connected with emotional development like, "Look or hear only what is good (for the fetus)" were practised little. Efforts for self-restraint as "Not to hate or pick out others' defects or talk bad about others" were appeared to be great, and it is practised well, too. 4. The subjects were well-informed on health area, but practised little. Behaviors for health improvement as to "Lead a regular daily life for health" or "Not to take a long trop" were practised very faithfully. The results of the survey showed that passive attitudes related to tabooed food, self-abstinence and cautions for bodily safety were prevalent; more positive and progressive disposition missing. That is, full perception and active practices for caloric intake, emotional improvement and physical strength development are needed.

• Journal of Aquaculture
• /
• v.11 no.3
• /
• pp.303-310
• /
• 1998

The growth and survival rate of larvae and juveniles from female rockfish (Sebastes schlegeli) broodstock in vitellogenesis by injection of 3,5,3'-triiodo-L-thyronine $T_3$ with a dose of 20 mg/kg fish wt. were examined for 30 days and compared with sham-control fish injected dime-thyl sulfoxide and control fish. Larvae were fed with rotifers for first 5 days after parturition, rotifers and Artemia nauplii for next 10 days. And then, they were fed with Artemia nauplii and commercial diet. Growth of larvae and juveniles from maternal $T_3$injection was significantly faster than that of two controls, nevertheless, the condition factor was lower than that of controls. The whole body proximate analyses indicated that there were significant effects of $T_3$ injection on protein and lipid, but no significant on moisture and ash contents. Survival rate of the fish from maternal $T_3$injection was significantly higher than that of fish from the two controls. These results indicate that thyroid hormone supplements appear to confer a distinct advantage to larval and juvenile rockfish in early, fragile development stage.