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우모 digest에 의한 taurine 강화 계육 생산

  • 이승민;임희석;백인기
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.11a
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    • pp.77-79
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    • 2003
  • The effects of dietary supplementation of feather meal digests(FM) and its digests on the growth of broiler chicks and taurine content in the broiler meat were examined. Total of 100 broiler chickens were assigned to five dietary treatments: T1; Control, T2; feather meal(FM) 5 % diet, T3; NaOH treated FM 5% diet, T4; HNO$_3$treated FM 5 % diet and T5; synthetic taurine 0.5 % supplemented diet. Taurine content of leg muscle was significantly(P<0.01) increased by treatments. The highest increase over the control was shown by 0.5 % taurine diet(170 %), followed by FM diet(123 %), NaOH treated FM diet(122 %) and HNO$_3$treated FM diet(63 %). Taurine content of breast muscle was increased by 246 % in 0.5 % taurine diet but FM diets were not significantly different from the control. Taurine content of heart muscle was not significantly affected by dietary treatments. There were big differences in the average taurine content of the parts or organ of the control birds; 778 $\mu\textrm{g}$/g leg muscle, 79 $\mu\textrm{g}$/g breast muscle and 1482 $\mu\textrm{g}$/g heart muscle. It was concluded that taurine content of leg muscle of broiler can be increased by supplementation of feather meal. Alkaline or acid treatment FM was not effective in improving taurine enrichment of the broiler meat.

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Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes

  • Zeinali, Majid;Ardestani, Sussan K.;Kariminia, Amina
    • Parasites, Hosts and Diseases
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    • v.45 no.4
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    • pp.287-293
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    • 2007
  • The identification and characterization of antigens that elicit human T cell responses is an important step toward understanding of Leishmania major infection and ultimately in the development of a vaccine. Micropreparative SDS-PAGE followed by electro transfer to a PVDF membrane and elution of proteins from the PVDF, was used to separate 2 novel proteins from L. major promastigotes, which can induce antibodies of the IgG2a isotype in mice and also are recognized by antisera of recovered human cutaneous leishmaniasis subjects. Fractionation of the crude extract of L. major revealed that all detectable proteins of interest were present within the soluble Leishmania antigens (SLA). Quantitation of these proteins showed that their expression in promastigotes is relatively very low. Considering the molecular weight, immunoreactivity, chromatographic and electrophoretic behavior in reducing and non-reducing conditions, these proteins are probably 2 isoforms of a single protein. A digest of these proteins was resolved on Tricine-SDS-PAGE and immunoreactive fragments were identified by human sera. Two immunoreactive fragments (36.4 and 34.8 kDa) were only generated by endoproteinase Glu-C treatment. These immunoreactive fragments or their parent molecules may be ideal candidates for incorporation in a cocktail vaccine against cutaneous leishmaniasis.

Consumer Needs for HMR Product Development of Chinese Elderly Consumers (중국 고령친화형 HMR 제품 개발을 위한 소비자 요구도)

  • Nam, Sang-Myung;Hong, Wan-Soo
    • Journal of the Korean Society of Food Culture
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    • v.36 no.5
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    • pp.441-454
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    • 2021
  • This study analyzed consumer needs for the development of elderly-friendly HMR products in China. In developing the products, items needed to be improved, types and packages of the necessary products, additional improvements and food preferences were investigated. According to the demographic characteristics of elderly Chinese consumers, these was analyzed as well. A survey was conducted on a total of 370 elderly people, and data analysis was performed using SPSS 18.0 program. Items related to product quality safety, such as "marks of origin by ingredient", "rich nutrients", "expansion of letter size of packaging", "rich nutrients" and "clear manufacturing date and expiration date", were required to develop HMR products. The types of products that should be developed were porridge and noodles, and the packaging types that should be developed were eco-friendly packaging and recycled packaging. In addition, "low-calorie HMR", "development of various seasonings", "use of eco-friendly food ingredients" and "HMR to digest easily" should be improved. The demand for product improvement and food preference showed significant differences according to the degree of education, monthly income and oral health. The results were intended to prepare basic data for setting the direction when developing elderly-friendly HMR.

Mapping of Quantitative Trait Loci for Yield and Grade Related Traits in Peanut (Arachis hypogaea L.) Using High-Resolution SNP Markers

  • Liang, Yuya;Baring, Michael R.;Septiningsih, Endang M.
    • Plant Breeding and Biotechnology
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    • v.6 no.4
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    • pp.454-462
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    • 2018
  • Yield and grade are the key factors that affect production value of peanut. The objective of this study was to identify QTLs for pod yield, hundred-seed weight, and total sound mature kernel (TSMK). A total of 90 recombinant inbred lines, derived from Tamrun OL07 and a breeding line Tx964117, were used as a mapping population and planted in Brownfield and Stephenville, Texas. A genetic map was developed using 1,211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-seq). A total of 10 QTLs were identified above the permutation threshold, three for yield, three for hundred-seed weight and four for TSMK, with LOD score values of 3.7 - 6.9 and phenotypic variance explained of 12.2% - 35.9%. Among those, there were several QTLs that were detected in more than one field experiment. The commonly detected QTLs in this study may be used as potential targets for future breeding program to incorporate yield and grade related traits through molecular breeding.

Enhancing anaerobic digestion of vegetable waste and cellulose by bioaugmentation with rumen culture

  • Jo, Yeadam;Hwang, Kwanghyun;Lee, Changsoo
    • Membrane and Water Treatment
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    • v.10 no.3
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    • pp.213-221
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    • 2019
  • Anaerobic digestion (AD) has been widely used to valorize food waste (FW) because of its ability to convert organic carbon into $CH_4$ and $CO_2$. Korean FW has a high content of fruits and vegetables, and efficient hydrolysis of less biodegradable fibers is critical for its complete stabilization by AD. This study examined the digestates from different anaerobic digesters, namely Rs, Rr, and Rm, as the inocula for the AD of vegetable waste (VW) and cellulose (CL): Rs inoculated with anaerobic sludge from an AD plant, Rr inoculated with rumen fluid, and Rm inoculated with anaerobic sludge and augmented with rumen fluid. A total of six conditions ($3\;inocula{\times}2\;substrates$) were tested in serial subcultures. Biogas yield was higher in the runs inoculated with Rm than in the other runs for both VW (up to 1.10 L/g VS added) and CL (up to 1.05 L/g VS added), and so was biogas production rate. The inocula had different microbial community structures, and both substrate type and inoculum source had a significant effect on the formation and development of microbial community structures in the subcultures. The overall results suggest that the bioaugmentation with rumen microbial consortium has good potential to enhance the anaerobic biodegradability of VW, and thereby can help more efficiently digest high fiber-content Korean FW.

Policies and industrial technology trends for senior-friendly foods (고령친화식품의 정책 및 산업기술 동향)

  • Lee, Hyun-Sun;Nam, Young-Joo;Kim, Yang-Eun;Kim, Jong-Chan;Shin, YunJeong;Lee, YoungJin;Heo, Wan
    • Food Science and Industry
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    • v.53 no.4
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    • pp.435-443
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    • 2020
  • Korea has entered into an aged-society in 2018. If this trend continues to increase, it is expected that a super-aging society will take place by 2026. Therefore, Korea is placed in the situation of becoming the world's fastest aging nation as it becomes a super-aging society from an aged-society in twenty-six years. In order to provide an effective supply of nutrition for the socially weak, Japan developed a variety of foods with food texture improvements. Germany improved accessibility for meal service development. Senior-friendly food is a softened food or a highly concentrated drink, which is considered a texture-modified food, for the elderly with eating disorders to digest food more easily by making food particles smaller. Varying food processing techniques such as freeze-thawing enzyme impregnation, high-pressure processing, super-heated steam processing, 3D food print, and others used to produce texture-modified foods.

DISEASE FORECAST USING MACHINE LEARNING ALGORITHMS

  • HUSSAIN, MOHAMMED MUZAFFAR;DEVI, S. KALPANA
    • Journal of applied mathematics & informatics
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    • v.40 no.5_6
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    • pp.1151-1165
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    • 2022
  • Key drive of information quarrying is to digest liked information starting possible information. With the colossal amount of realities kept in documents, information bases, and stores, in the medical care area, it's inexorably significant, assuming excessive, arising compelling resources aimed at examination besides comprehension like information on behalf of the withdrawal of gen that might assistance in independent direction. Classification is method in information mining; it's characterized as per private, passing on item toward a specific course established happening it is likeness toward past instances of different substances trendy the data collection. In pre-owned recycled four Classification algorithm that incorporate Multi-Layer perception, KSTAR, Bayesian Network and PART to fabricate the grouping replicas arranged the malaria data collection and analyze the replicas, degree their exhibition through Waikato Environment for Knowledge Analysis introduced to Java Development Kit 8, then utilizations outfit's technique trendy promoting presentation of the arrangement methodology. The outcome perceived that Bayesian Network return most elevated exactness of 50.05% when working on followed by Multi-Layer perception, with 49.9% when helping is half, then, at that point, Kstar with precision of 49.44%, 49.5% when supporting individually and PART have lesser precision of 48.1% when helping, The exploration recommended that Bayesian Network is awesome toward remain utilized on Malaria data collection in our sanatoriums.

Design and Implementation of the Document HTML System for Preserving Content Integrity

  • Hyun Cheon Hwang;Ji Su Park;Jin Gon Shon
    • Journal of Information Processing Systems
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    • v.19 no.3
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    • pp.334-346
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    • 2023
  • An electronic document based on PDF has been widely used in customer communication between an enterprise and a customer to deliver personalized content. However, electronic documents based on PDF in the form of paper layouts are not suitable for mobile environments because of low readability and lack of interactive interaction. Even though HTML is an essential language in a mobile environment, electronic document based on PDF is still used as it has a content integrity verification feature with a digital signature. It means that a user is sacrificing user experience in a mobile environment for content integrity and using paper-layout electronic documents. In this research, we design the Document HTML specification by setting the Document HTML conformance, adding the extended meta tags, and signing the message digest with a digital signature based on public key infrastructure (PKI). Furthermore, we implemented the Document HTML system, which has REST API services to generate and verify the Document HTML, and did experimental verification of the theory. As a result, we have confirmed that the Document HTML has both content integrity and user experience on mobile. Furthermore, the Document HTML is expected to be an alternative document format to deliver personalized content from an enterprise to a customer in a mobile environment instead of the paper layout electronic document such as PDF.

Phagocytosis-associated genes in Acanthamoeba castellanii feeding on Escherichia coli

  • Min-Jeong Kim;Eun-Kyung Moon;Hye-Jeong Jo;Fu-Shi Quan;Hyun-Hee Kong
    • Parasites, Hosts and Diseases
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    • v.61 no.4
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    • pp.397-404
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    • 2023
  • Acanthamoeba species are free-living amoebae those are widely distributed in the environment. They feed on various microorganisms, including bacteria, fungi, and algae. Although majority of the microbes phagocytosed by Acanthamoeba spp. are digested, some pathogenic bacteria thrive within them. Here, we identified the roles of 3 phagocytosis-associated genes (ACA1_077100, ACA1_175060, and AFD36229.1) in A. castellanii. These 3 genes were upregulated after the ingestion of Escherichia coli. However, after the ingestion of Legionella pneumophila, the expression of these 3 genes was not altered after the consumption of L. pneumophila. Furthermore, A. castellanii transfected with small interfering RNS (siRNA) targeting the 3 phagocytosis-associated genes failed to digest phagocytized E. coli. Silencing of ACA1_077100 disabled phagosome formation in the E. coli-ingesting A. castellanii. Alternatively, silencing of ACA1_175060 enabled phagosome formation; however, phagolysosome formation was inhibited. Moreover, suppression of AFD36229.1 expression prevented E. coli digestion and consequently led to the rupturing of A. castellanii. Our results demonstrated that the ACA1_077100, ACA1_175060, and AFD36229.1 genes of Acanthamoeba played crucial roles not only in the formation of phagosome and phagolysosome but also in the digestion of E. coli.

Evaluation of the impact of phytase supplementation on growth performance, nutrient digestibility, and fecal score of growing pigs

  • Shanshui Gao;Md Mortuza Hossain;In Ho Kim
    • Korean Journal of Agricultural Science
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    • v.50 no.4
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    • pp.653-661
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    • 2023
  • Phytase enhances phosphorus availability in pig diets by breaking down phytic acid, which is challenging for animals to digest. This study aimed to assess the impact of dietary phytase supplementation on the growth performance, nutrient digestibility, and fecal score of growing pigs. Sixty-four growing pigs ([Yorkshire × Landrace] × Duroc, average body weight 21.20 ± 0.18 kg) were randomly assigned to one of two treatment groups. The dietary treatments were CON, basal diet, and PHY, basal diet + 0.0025% phytase. Results indicated a tendency for increased average daily gain in the phytase-supplemented diet group (p < 0.10) compared to the control diet group. The average daily feed intake and feed conversion ratio were not affected by the addition of 0.0025% phytase. Furthermore, dry matter digestibility, nitrogen content, and digestible energy were not influenced (p > 0.05) by phytase supplementation. Fecal score remained similar (p > 0.05) in growing pigs fed phytase-supplemented and control diets. These findings suggest that supplementing the diet of growing pigs with 0.0025% phytase may lead to improved average daily gain without adverse effects on nutrient digestibility and fecal score. This implies the potential to enhance growth performance without compromising overall health or digestion in pigs. Further investigations into the optimal levels of phytase supplementation for growing pigs are recommended.