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Mycological Characteristics and Carbon Source Activities of Unrecorded Wild Yeast Species from Vineyard Soil in Korea (국내 포도밭 토양에서 분리된 야생 효모 미기록종들의 균학적 특성과 탄소원 활성)

  • Bong Soon Yim;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.373-381
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    • 2023
  • This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.

Formation of Secondary Products by Plant Cell Culture - II. Effects of Growth Regulators on the Formation of Capsaicinoide, Phenylpropanoids and PAL Activity in Cultured Cell of Capsicum annuum L. - (식물세포(植物細胞) 배양(培養)에 의(依)한 이차대사산물(二次代謝産物)의 생성(生成)에 관(關)한 연구(硏究) - II. Capsicum annuum L.의 배양세포(培養細胞)에 있어서 Growth Regulator가 Capsaicinoids, Phenylpropanoids 생성(生成) 및 Phenylalanine Amnonia-lyase (PAL) 활성(活性)에 미치는 영향 -)

  • Choi, Bong-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.1
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    • pp.10-17
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    • 1987
  • In order to investigate the effects of growth regulators on the formation of capsaicinoids in callus of Capsicum annuum L. tissues were cultured in the Linsmaier and Skoog RM 1964 medium containing various growth regulators. Production of capsaicinoids during culture was monitored by gas chromatography. In the presence of $10^{-6}M$ of 2,4-D and kinetin in the medium, $1182{\mu}g$ of capsaicinoids were formed per 100g dry wt. of tissue, of which was greater than with any of three other growth regulators. IAA, NAA, and kinetin of same concentrations had 65%, 38%, 68% effect of 2.4-D in capsaicinoids formation, respectively. Production of capsaicinoids increased gradually in the presence of 2,4-B as culture period was proceeded. Of phenylpropanoids formed, cinnamic acid and coumaric acid were not significantly different in their levels, although growth regulators were varied. On the other hand, caffeic acid and ferulic acid formation were highest in the presence of 2,4-D. Effects of kinetin and IAA were about 70 percent of that of 2,4-D, whereas NAA had only about 30 percent effect. Phenylalanine ammonia-lyase activity in cultured tissue was increased during the periods; 52, 81, and 209 n moles of cinnamic acid per g fresh wt. were formed after 5, 15, and 25 days of culture, respectively.

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Small Bowel Sparing Effect of Small Bowel Displacement System in 3D-CRT and IMRT for Cervix Cancer (자궁경부암의 3D-CRT와 IMRT시 소장전위장치의 소장 선량에 대한 영향)

  • Kang, Min-Kyu;Huh, Seung-Jae;Han, Young-Yih;Park, Won;Ju, Sang-Gyu;Kim, Kyoung-Ju;Lee, Jeung-Eun;Park, Young-Je;Nam, Hee-Rim;Lim, Do-Hoon;Ahn, Yong-Chan
    • Radiation Oncology Journal
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    • v.22 no.2
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    • pp.130-137
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    • 2004
  • Purpose : In radiotherapy for cervix cancer, both 3-dimensioal radiotherapy (3D-CRT) and intensity-modulated radiation therapy (IMRT) could reduce the dose to the small bowel (SB), while the small bowel displacement system (SBDS) could reduce the SB volume in the pelvic cavity. To evaluate the effect of the SBDS on the dose to the SB in 3D-CRT and IMRT plans, 3D-CRT and IMRT plans, with or without SBDS, were compared. Materials and Methods : Ten consecutive uterine cervix cancer patients, receiving curative radiotherapy, were accrued. Ten pairs of computerized tomography (CT) scans were obtained in the prone position, with or without SBDS, which consisted of a Styrofoam compression device and an individualized custom-made abdominal immobilization device. Both 3D-CRT, using the 4-field box technique, and IMRT plans, with 7 portals of 15 MV X-ray, were generated for each CT image, and proscribed 50 Gy (25 fractions) to the isocenter. For the SB, the volume change due to the SBDS and the DVHs of the four different plans were analyzed using palled t-tests. Results : The SBDS significantly reduced the mean SB volume from 522 to 262 cm$^{3}$ (49.8$\%$ reduction). The SB volumes that received a dose of 10$\~$50 Gy were significantly reduced in 3D-CRT (65$\~$80$\%$ reduction) and IMRT plans (54$\~$67$\%$ reduction) using the SBDS. When the SB volumes that received 20$\~$50 Gy were compared between the 3D-CRT and IMRT plans, those of the IMRT without the SBDS were significantly less, by 6$\~$7$\%$, than those for the 3D-CRT without the SBDS, but the volume difference was less than 1$\%$ when using the SBDS. Conclusion : The SBDS reduced the radiation dose to the SB in both the 3D-CRT and IMRT plans, so could reduce the radiation injury of the SB.

Reduction in the contents of acetaldehyde, methanol and fusel alcohols in the Muscat Bailey A wine fermented by Korean indigenous sugar-tolerant yeasts Saccharomyces cerevisiae S13 and D8 (토착형 아황산 및 당 내성 효모 Saccharomyces cerevisiae S13과 D8에 의한 Muscat Bailey A 포도주의 아세트알데히드, 메탄올 및 고급알코올의 감소 효과)

  • Kim, Mi-Sun;Park, Heui-Dong
    • Food Science and Preservation
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    • v.21 no.6
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    • pp.851-858
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    • 2014
  • Muscat Bailey A (MBA) wine was fermented using the indigenous Korean Saccahromyces cerevisiae strains S13 and D8, and the fermentation characteristics were compared with those of S. cerevisiae W-3, an industrial wine yeast. The strains S13 and D8 showed delayed alcohol fermentation compared with the W-3 strain, but the final alcohol contents of the S13 and D8 wines after fermentation were similar to those of the W-3 wine. The S13 wine showed significantly lower malic-acid content than the W-3 wine, but the D8 wine showed a similar level. Both the wines fermented using the S13 and D8 strains showed significantly lower acetaldehyde, methanol, and fusel oil contents, including n-propanol, iso-butyl alcohol, and iso-amyl alcohol, compared to the W-3 wine. Especially, the methanol content was 98.6 mg/L in the S13 wine and 112.0 mg/L in the D8 wine, which were much lower than 192.8 mg/L in the W-3 wine. The S13 wine obtained the highest score in terms of color among the three wines in the sensory evaluation, with lower Hunter's L, a, and b values compared to the W-3 wine.

Fatigue Test of 3D-printed ABS Parts Fabricated by Fused Deposition Modeling (FDM 방식으로 제작된 ABS 재료의 피로 특성 평가)

  • Seol, Kyoung-Su;Shin, Byoung-Chul;Zhang, Sung-Uk
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.17 no.3
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    • pp.93-101
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    • 2018
  • FDM is one of the popular 3D printing technologies because of an inexpensive extrusion machine and multi-material printing. FDM can use thermoplastics such as ABS and PLA. The 3D-printed ABS parts fabricated by FDM are attractive in the automotive industry because of their weight. A 10% reduction in weight can increase the fuel economy by approximately 7%. To use 3D-printed ABS parts as automotive parts, we should evaluate the 3D-printed parts in terms of automotive reliability. In this study, 3D-printed ABS samples were evaluated using Ono's rotary bending fatigue test. We obtained an S-N curve for the 3D-printed ABS specimen from the finite-element analysis. The S-N curve can be useful in early-stage design decisions for 3D-printed ABS parts.

A 10-bit 40-MS/s Low-Power CMOS Pipelined A/D Converter Design (10-bit 40-MS/s 저전력 CMOS 파이프라인 A/D 변환기 설계)

  • Lee, Sea-Young;Yu, Sang-Dae
    • Journal of Sensor Science and Technology
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    • v.6 no.2
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    • pp.137-144
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    • 1997
  • In this paper, the design of a 10-bit 40-MS/s pipelined A/D converter is implemented to achieve low static power dissipation of 70 mW at the ${\pm}2.5\;V$ or +5 V power supply environment for high speed applications. A 1.5 b/stage pipeline architecture in the proposed ADC is used to allow large correction range for comparator offset and perform the fast interstage signal processing. According to necessity of high-performance op amps for design of the ADC, the new op amp with gain boosting based on a typical folded-cascode architecture is designed by using SAPICE that is an automatic design tool of op amps based on circuit simulation. A dynamic comparator with a capacitive reference voltage divider that consumes nearly no static power for this low power ADC was adopted. The ADC implemented using a $1.0{\mu}m$ n-well CMOS technology exhibits a DNL of ${\pm}0.6$ LSB, INL of +1/-0.75 LSB and SNDR of 56.3 dB for 9.97 MHz input while sampling at 40 MHz.

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Steroidal Saponins from the Rhizomes of Asparagus oligoclonos and their Antibacterial Activity (방울비짜루(Asparagus oligoclonos)로부터 분리한 스테로이드 사포닌의 항균활성)

  • Seong, Jae-Duck;Park, Seung-Yong;Oh, Sei-Ryang;Kwack, Yong-Ho;Kim, Geum-Soog
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.136-140
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    • 2000
  • Two steroidal saponins were isolated from MeOH extract of the rhizomes of Asparagus oligoclonos. Using the spectroscopic techniques of $^1H,\;^{13}C$ NMR and 2D NMR, they were identified as $3-O-[{\beta}-D-glucopyranosyl-(1{\rightarrow}2)-{\beta}-D-glucopyranosyl]-(25S)-spirostan-3{\beta}-ol$ and $3-O-{{\beta}-D-glucopyranosyl-(1{\rightarrow}2)-[{\beta}-D-xylopyranosyl-(1{\rightarrow}4)]-{\beta}-D-glucopyranosyl}-(25S)-spirostan-3{\beta}-ol$, respectively. They were first isolated from A. oligoclonos. The in vitro antibacterial activities as well as antibacterial spectrums against 20 bacteria strains were investigated with MIC test. Both saponins inhibited the growth of 10 bacteria strains at the concentration of$100\;{\mu}g/ml$.

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3DTV System Adaptive to User's Environment (사용자 환경에 적응적인 3DTV 시스템)

  • Baek, Yun-Ki;Choi, Mi-Nam;Park, Se-Whan;Yoo, Ji-Sang
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.10C
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    • pp.982-989
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    • 2007
  • In this paper, we propose a 3DTV system that considers user's view point and display environment. The proposed system consists of 3 parts - multi-view encoder/decoder, face-tracker, and 2D/3D converter. The proposed system try to encode multi-view sequence and decode it in accordance with the user's view point and it also gives a stereopsis to the multi-view image by using of 2D/3D conversion which converts decoded two-dimensional(2D) image to three-dimensional(3D) image. Experimental results shows that we are able to correctly reconstruct a stereoscopic view that is exactly corresponding to user's view point.

Recent R&D Trends for 3D Deep Learning (3D 딥러닝 기술 동향)

  • Lee, S.W.;Hwang, B.W.;Lim, S.J.;Yoon, S.U.;Kim, T.J.;Choi, J.S.;Park, C.J.
    • Electronics and Telecommunications Trends
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    • v.33 no.5
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    • pp.103-110
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    • 2018
  • Studies on artificial intelligence have been developed for the past couple of decades. After a few periods of prosperity and recession, a new machine learning method, so-called Deep Learning, has been introduced. This is the result of high-quality big- data, an increase in computing power, and the development of new algorithms. The main targets for deep learning are 1D audio and 2D images. The application domain is being extended from a discriminative model, such as classification/segmentation, to a generative model. Currently, deep learning is used for processing 3D data. However, unlike 2D, it is not easy to acquire 3D learning data. Although low-cost 3D data acquisition sensors have become more popular owing to advances in 3D vision technology, the generation/acquisition of 3D data remains a very difficult problem. Moreover, it is not easy to directly apply an existing network model, such as a convolution network, owing to the variety of 3D data representations. In this paper, we summarize the 3D deep learning technology that have started to be developed within the last 2 years.

Regeneration and Maturation Induction for the Free-living Gametophytes of Ecklonia cava Kjellman (Laminariales, Phaeophyta) (대형갈조류 감태(Ecklonia cava Kjellman) 유리배우체의 재생 및 성숙 유도)

  • Wi, Mi-Young;Hwang, Eun-Kyoung;Kim, Seong-Cheol;Hwang, Mi-Sook;Baek, Jae-Min;Park, Chan-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.5
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    • pp.381-388
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    • 2008
  • Induction of regeneration and maturation for the free-living gametophytes of Ecklonia cava Kjellman was conducted under various temperature, irradiance and photoperiod conditions. Culture conditions for female or male gametophyte fragments were 4 temperature (5, 10, 15 and $20^{\circ}C$), 4 irradiance (5, 10, 20 and $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and 3 photoperiod (14:10, 12:12 and 10:14 h L:D). Female and male gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, $5{\sim}10{\mu}mol{\cdot}m-2{\cdot}s-1$, 12:12h or 10:14h (L:D) and $15-20^{\circ}C$, $20-40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 14:10h (L:D), respectively. Sexual maturation of female and male gametophytes facilitated under $15^{\circ}C$, $20-40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 14:10h (L:D) and $5-10^{\circ}C$, $5-10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 10:14h (L:D), respectively. These results provide basic informations to control the regeneration or maturation of the free-living gametophytes for artificial seed production of E. cava.