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http://dx.doi.org/10.5657/kfas.2008.41.5.381

Regeneration and Maturation Induction for the Free-living Gametophytes of Ecklonia cava Kjellman (Laminariales, Phaeophyta)  

Wi, Mi-Young (Seaweed Research Center, NFRDI)
Hwang, Eun-Kyoung (Seaweed Research Center, NFRDI)
Kim, Seong-Cheol (Seaweed Research Center, NFRDI)
Hwang, Mi-Sook (Seaweed Research Center, NFRDI)
Baek, Jae-Min (Seaweed Research Center, NFRDI)
Park, Chan-Sun (Department of Marine and Fisheries Resources, Mokpo National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.41, no.5, 2008 , pp. 381-388 More about this Journal
Abstract
Induction of regeneration and maturation for the free-living gametophytes of Ecklonia cava Kjellman was conducted under various temperature, irradiance and photoperiod conditions. Culture conditions for female or male gametophyte fragments were 4 temperature (5, 10, 15 and $20^{\circ}C$), 4 irradiance (5, 10, 20 and $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and 3 photoperiod (14:10, 12:12 and 10:14 h L:D). Female and male gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, $5{\sim}10{\mu}mol{\cdot}m-2{\cdot}s-1$, 12:12h or 10:14h (L:D) and $15-20^{\circ}C$, $20-40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 14:10h (L:D), respectively. Sexual maturation of female and male gametophytes facilitated under $15^{\circ}C$, $20-40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 14:10h (L:D) and $5-10^{\circ}C$, $5-10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 10:14h (L:D), respectively. These results provide basic informations to control the regeneration or maturation of the free-living gametophytes for artificial seed production of E. cava.
Keywords
Ecklonia cava; Free-living gametophyte; Regeneration; Maturation;
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1 Athukorala, Y., K.N. Kim and Y.J. Jeon. 2006. Antipro- liferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and Chemical Toxicology, 44, 1065-1074   DOI   ScienceOn
2 Brown, M.T. and M.D. Lamare. 1994. The distribution of Undaria pinnatifida (Harvey) Suringar within Timaru Habour, New Zealand. Jpn. J. Phycol., 42, 63-70
3 Edding, M.E. and F.B. Tala. 2003. Development of techniques for the cultivation of Lessonia trabeculata Villouta et Santelices (Phaeophyceae: Laminariales) in Chile. Aqua. Res., 34, 507-515   DOI   ScienceOn
4 Luning, K. 1980. Critical levels of light and temperature regulating the gametogenesis of three Laminaria species (Phaeophyceae). J. Phycol., 16, 1-15   DOI
5 Novaczek, I. and J. McLachlan. 1987. Correlation of tem- perature and daylength responses of Sphaerotrichia divaricata (Phaeophyta, Chordariales) with field phenology in Nova Scotia and distribution in eastern North America. Br. Phycol. J., 22, 215-219   DOI
6 Serisawa, Y., Y. Yokohama, Y. Aruga and J. Tanaka. 2002. Growth of Ecklonia cava (Laminariales, Phaeophyta) sporophytes transplanted to a locality with different temperature conditions. Phycol. Res., 50, 201-207   DOI
7 Hori, T. 1993. An illustrated atlas of life history of algae. Vol. 2 Brown and red algae. Uchida Rokakuho Publ., Tokyo, 1-345
8 Maier, I. 1982. New aspects of pheromone-triggered sper- matozoid release in Laminaria digitata (Phaeophyta). Protoplasma, 113, 137-143   DOI
9 Parker, R.E. 1979. Introductory statistics for biology. 2nd edition. Edward Arnold, London, 1-122
10 Kim, D.S., J.P. Hong., Y.D. Kim., H.I. Song. and H.G. Kim. 2007. Seed production and cultivation of Ecklonia stolonifera Okamura, Phaeophyta. J. Kor. Fish Soc., 20, 7-13   과학기술학회마을
11 Zar, J.H. 1984. Biostatistical analysis. Prentice Hall, Engelwood Cliffs, N.J., 1-718
12 Tatewaki, M. 1966. Formation of a crustaceos sporophyte with unilocular sporangia in Scytosiphon lomentaria. Phycologia, 6, 62-66   DOI
13 Fain, S.R. and S.N. Murray. 1982. Effects of light and temperature on net photosynthesis and dark respiration of gametophytes and embryonic sporphytes of Macrocystis pyrifera. J. Phycol., 18, 92-98   DOI
14 Gerard, V.A. 1984. The light environment in a giant kelp forest: influence of Macrocystis pyrifera on spatial and temporal variability. Mar. Biol., 84. 189-195   DOI
15 Kain, J.M. 1964. Aspects of the biology of Laminaria hyperborea. III. Survival and growth of gametophytes. J. Mar. Biol. Assoc. U.K., 44, 415-433   DOI
16 Ar Gall, E., A. Asensi, D. Marie and B. Kloareg. 1996. Parthenogenesis and apospory in the Laminariales: a flow cytometry analysis. Eur. J. Phycol., 31, 369-380   DOI   ScienceOn
17 Hong, J.H., B.S. Son, B.K. Kim, H.Y. Chee, K.S. Song, B.H. Lee, H.C. Shin and H.C. Lee. 2006. Antihyper- tensive effect of Ecklonia cava extract. Korean J. Pharmacol., 37, 200-205
18 Lewis, R.J., B.Y. Jiang, M. Neushul and X.G. Fei. 1993. Haploid parthenogenetic sporophytes of Laminaria japonica (Phaeophyceae). J. Phycol., 29, 363-369   DOI   ScienceOn
19 Bolton, L.L. and G.J. Lewitt. 1985. Light and temperature requirements for growth and reproduction in gameto- phytes of Ecklonia maxina (Alariceae, Laminariales). Mar. Biol., 87, 131-135   DOI
20 Leukart, P. and K. Lüning. 1994. Minimum spectral light requirements and maximum light levels for long-term germling growth of several red algae from different water depth and a green alga. Eur. J. Phycol., 29, 103-112   DOI   ScienceOn
21 Kang, L.S. and S.J. Yoo. 1993. The acute toxity of three oils to the early life of Undaria pinnatifida (Harvey) Suringar. Korean J. Phycol., 8, 77-82
22 Westermeier, R., D. Patino, M.I. Piel, I. Maier and D.G. Mueller. 2006. A new approch to kelp mariculture in Chile: production of free-floating sporophyte seedlings from gametophyte cultures of Lessonia trabeculata and Macrocystis pyrifera. Aqua. Res., 37, 164-171   DOI   ScienceOn