• BMB Reports
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• v.40 no.1
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• Bulletin of the Korean Mathematical Society
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• v.20 no.1
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• pp.15-25
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• 1983

The main purpose of the present paper is to generalize the results obtained by A. Hindmarsh in [7] to the holomorphic functions with non-negative real part defined on a complete circular domain D in certain class D in the complex euclidean space $C^{n}$. As described in .cint.2, D includes the bounded symmetric domains.s.

• Journal of Communications and Networks
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• v.16 no.4
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• pp.447-457
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• 2014

In this paper, we investigate the frequency domain channel estimation for multiple-input multiple-output (MIMO) single-carrier frequency-division multiple-access (SC-FDMA) systems. In MIMO SC-FDMA, code-division multiplexed (CDM) pilots such as cyclic-shifted Zadoff-Chu sequences have been adopted for channel estimation. However, most frequency domain channel estimation schemes were developed based on frequency-division multiplexing of pilots. We first develop a channel estimation error model by using CDM pilots, and then analyze the mean-square error (MSE) of various minimum MSE (MMSE) frequency domain channel estimation techniques. We show that the cascaded one-dimensional robust MMSE (C1D-RMMSE) technique is complexity-efficient, but it suffers from performance degradation due to the channel correlation mismatch when compared to the two-dimensional MMSE (2D-MMSE) technique. To improve the performance of C1D-RMMSE, we design a robust iterative channel estimation (RITCE) with a frequency replacement (FR) algorithm. After deriving the MSE of iterative channel estimation, we optimize the FR algorithm in terms of the MSE. Then, a low-complexity adaptation method is proposed for practical MIMO SC-FDMA systems, wherein FR is performed according to the reliability of the data estimates. Simulation results show that the proposed RITCE technique effectively improves the performance of C1D-RMMSE, thus providing a better performance-complexity tradeoff than 2D-MMSE.

• Biomolecules & Therapeutics
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• v.24 no.5
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• pp.475-481
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• 2016

PICK1, a PDZ domain-containing protein, is known to increase the reuptake activities of dopamine transporters by increasing their expressions on the cell surface. Here, we report a direct and functional interaction between PICK1 and dopamine $D_3$ receptors ($D_3R$), which act as autoreceptors to negatively regulate dopaminergic neurons. PICK1 colocalized with both dopamine $D_2$ receptor ($D_2R$) and $D_3R$ in clusters but exerted different functional influences on them. The cell surface expression, agonist affinity, endocytosis, and signaling of $D_2R$ were unaffected by the coexpression of PICK1. On the other hand, the surface expression and tolerance of $D_3R$ were inhibited by the coexpression of PICK1. These findings show that PICK1 exerts multiple effects on $D_3R$ functions.

• Korean Journal of Mathematics
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• v.16 no.1
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• pp.25-40
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• 2008

We are concerned with a free boundary problem for the 2D Stokes channel flows, which determines the profile of the wing for the channel, so that the given traction force is to be distributed along the wing of the channel. Using the domain embedding technique, the free boundary problem is transferred into the shape optimization problem through the compliant formulation by releasing the traction condition along the variable boundary. The justification of the formulation will be discussed.

• Journal of electromagnetic engineering and science
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• v.8 no.4
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• pp.139-143
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• 2008

In this paper, a new scheme to calculate the weighting factor of the 2-D isotropic-dispersion finite difference time domain(ID-FDTD) is proposed. The weighting factor in [1] was formulated in free space, so that it may not be optimal in dielectric media. Therefore, the weighting factor was reformulated by considering the material properties and using the least mean square method. As a result, a minimum numerical dispersion error for any dielectric media is guaranteed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.3B
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• pp.541-548
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• 2000

In the MPEG-4 standardization phase, many methods for coding the irregular shaped VOP (video object Plane) have been researched. Texture coding is one of interesting research items in the MPEG-4. There are the Low pass extrapolation (LPE) padding, the shape adaptive DCT (SA-DCT), and the Extension-Interpolation (EI)/2D-DCT proposed in [1] as texture coding methods. the EI/2D-DCT is the method extending and interpolating luminance values from and Arbitrarily Shaped (AS) image segment into an 8 x 8 block and transforming the extended and interpolated luminance values by the 8x8 DCT. although the EI/2D-DCT and the SA-DCT work well in coding the As image segments. they are degraded since they use one-dimensional (1-D) methods such as the 1D-EI and the 1D-DCT in the two-dimensional (2-D) space. in this paper, we analyze the performance of the EI/2D-DCTand propose a new non-symmetric sig-sag scanning method, which non-symmetrically scans the quantized coefficients in the DCT domain to improve the EI/2D-DCT.

• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1043-1046
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• 2009

Human protein tyrosine kinase-6 (PTK6) is a member of the non-receptor protein tyrosine kinase family and it is found in two-thirds of all breast tumors. Very recently, we proposed that the SH3 domain of PTK6 interacts with the linker region (Linker) between the SH2 and kinase domains, proving that the interaction between SH3 domain and Linker plays an important role in auto-inhibition mechanism. Residues from 1 to 191 corresponding region of SH3-SH2-Linker (SH32L) of PTK6 was cloned into the pET32a expression vector with Tobbaco etch virus (TEV) protease enzyme site by sequence homology and 3D structural model. The purified PTK6-SH32L was determined as a monomer conformation in solution. The amide proton resonances in the $^{15}N-^{1}H$ 2D-HSQC spectrum suggest that PTK6-SH32L possesses disordered structural region of the flexible/unstructured linker region. In addition, the backbone amide proton chemical shifts of the SH3 domain in the PTK6-SH32L differ from that of the independent domain, indicating that intra-molecular interaction between SH3 and Linker in the PTK6-SH32L is present.

• Journal of electromagnetic engineering and science
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• v.9 no.1
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• pp.46-52
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• 2009

This paper conducts a study on the frequency-dependent filtering and blocking effects of a variety of periodic structures, dubbed frequency selective surface(FSS). The periodic structures of interest are 1D and 2D repeated patterns of metal patches or slots sitting on the interface between the two different regions in the layered media which will show the capacitive or inductive behaviors and incorporated with the electromagnetic bandgap(EBG) geometry as another stratified media. Besides the normal substances so called double positive(DPS)-type in the layered media, metamaterials of double negative(DNG) are considered as layering components on the purpose of investigating the unusual electromagnetic phenomena. Frequency responses of transmission(absorption in terms of scattering) and reflection will be calculated by a numerical analysis which can be validated by the comparison with the open literature and demonstrated for the periodic structures embedding metamaterials or not. Most importantly, numerous examples of FSS will present the useful guidelines to have absorption or reflection properties in the frequency domain.

• Environmental Analysis Health and Toxicology
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• v.17 no.1
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• pp.73-80
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• 2002

Pyruvate dehydrogenase phosphatase (PDP)와 kinase는 당대사시 해당과정에서의 대사 산물인 pyruvate를 acetyl CoA로 만들어 구연산 회로로 진입시켜 주는 효소인 pyruvate dehydrogenase complex (PDC)의 활성을 조절하는 중요한 효소이다. PDP의 catalytic subunit는 PDC의 dihydrolipoamide acetyltransferase (E2), PDP regulatory subunit (PDPr), 그리고 칼슘 결합 도메인 등으로 구성되어 있는 것으로 추측되어지고 있다. 본 연구에서는 그 구조와 기능과의 상관관계를 알아보기 위해 PDPc를 E. coli JM101에서 발현시켜 순수 정제 후 단백분해 효소를 이용한 제한적 가수분해 방법을 이용해 그 구조와 기능과의 상관관계에 대해 연구하고자 하였다 정제된 PDPc는 trypsin, chymotrypsin, Arg-C 그리고 elastase를 이용하여 3$0^{\circ}C$ 그리고 pH 7.0에서 제한적으로 분해시켰으며 각 분해산물의 아미노 말단의 아미노산 배열을 분석하였다. 그 결과 PDPc는 trypsin, chymotrypsin, elastase에 의해 N-terminal의 50 kD과 C-terminal의 10 kD의 두개의 분해산물을 만들었으며, Arg-C에 의해 50kD의 분해산물은 약 35kD와 15kD으로 더 가수분해가 되었다. 이러한 결과로 볼 때 PDPc는 앞에서 추측한데로 세개의 주요한 기능적 도메인으로 이루어져 있음을 알 수 있었다 또한 C-terminal의 10kD은 PDPc의 활성에는 영향을 주지 않는 것으로 밝혀졌으나 다른 도메인의 기능은 더 연구가 되어져야 할 것으로 생각된다.