Browse > Article
http://dx.doi.org/10.4062/biomolther.2016.015

Functional Regulation of Dopamine D3 Receptor through Interaction with PICK1  

Zheng, Mei (Pharmacology Laboratory, College of Pharmacy, Chonnam National University)
Zhang, Xiaohan (Pharmacology Laboratory, College of Pharmacy, Chonnam National University)
Min, Chengchun (Pharmacology Laboratory, College of Pharmacy, Chonnam National University)
Choi, Bo-Gil (Medicinal Chemistry Laboratory, College of Pharmacy, Chonnam National University)
Oh, In-Joon (Physical Pharmacy Laboratory, College of Pharmacy, Chonnam National University)
Kim, Kyeong-Man (Pharmacology Laboratory, College of Pharmacy, Chonnam National University)
Publication Information
Biomolecules & Therapeutics / v.24, no.5, 2016 , pp. 475-481 More about this Journal
Abstract
PICK1, a PDZ domain-containing protein, is known to increase the reuptake activities of dopamine transporters by increasing their expressions on the cell surface. Here, we report a direct and functional interaction between PICK1 and dopamine $D_3$ receptors ($D_3R$), which act as autoreceptors to negatively regulate dopaminergic neurons. PICK1 colocalized with both dopamine $D_2$ receptor ($D_2R$) and $D_3R$ in clusters but exerted different functional influences on them. The cell surface expression, agonist affinity, endocytosis, and signaling of $D_2R$ were unaffected by the coexpression of PICK1. On the other hand, the surface expression and tolerance of $D_3R$ were inhibited by the coexpression of PICK1. These findings show that PICK1 exerts multiple effects on $D_3R$ functions.
Keywords
Dopamine $D_2$ receptor; Dopamine $D_3$ receptor; Dopamine transporter; PICK1; Cell surface expression; Tolerance;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Cho, E. Y., Cho, D. I., Park, J. H., Kurose, H., Caron, M. G. and Kim, K. M. (2007) Roles of protein kinase C and actin-binding protein 280 in the regulation of intracellular trafficking of dopamine D3 receptor. Mol. Endocrinol. 21, 2242-2254.   DOI
2 Dev, K. K., Nakajima, Y., Kitano, J., Braithwaite, S. P., Henley, J. M. and Nakanishi, S. (2000) PICK1 interacts with and regulates PKC phosphorylation of mGLUR7. J. Neurosci. 20, 7252-7257.   DOI
3 Diaz, J., Pilon, C., Le Foll, B., Gros, C., Triller, A., Schwartz, J. C. and Sokoloff, P. (2000) Dopamine D3 receptors expressed by all mesencephalic dopamine neurons. J. Neurosci. 20, 8677-8684.   DOI
4 Ferguson, S. S. (2007) Phosphorylation-independent attenuation of GPCR signalling. Trends Pharmacol. Sci. 28, 173-179.   DOI
5 Gurevich, E. V. and Joyce, J. N. (1999) Distribution of dopamine D3 receptor expressing neurons in the human forebrain: comparison with D2 receptor expressing neurons. Neuropsychopharmacology 20, 60-80.   DOI
6 Hausdorff, W. P., Caron, M. G. and Lefkowitz, R. J. (1990) Turning off the signal: desensitization of beta-adrenergic receptor function. FASEB J. 4, 2881-2889.   DOI
7 Hirbec, H., Perestenko, O., Nishimune, A., Meyer, G., Nakanishi, S., Henley, J. M. and Dev, K. K. (2002) The PDZ proteins PICK1, GRIP, and syntenin bind multiple glutamate receptor subtypes. Analysis of PDZ binding motifs. J. Biol. Chem. 277, 15221-15224.   DOI
8 Kim, K. M., Valenzano, K. J., Robinson, S. R., Yao, W. D., Barak, L. S. and Caron, M. G. (2001) Differential regulation of the dopamine D2 and D3 receptors by G protein-coupled receptor kinases and betaarrestins. J. Biol. Chem. 276, 37409-37414.   DOI
9 Sokoloff, P., Giros, B., Martres, M. P., Bouthenet, M. L. and Schwartz, J. C. (1990) Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics. Nature 347, 146-151.   DOI
10 Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M., Chishti, A. H., Crompton, A., Chan, A. C., Anderson, J. M. and Cantley, L. C. (1997) Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science 275, 73-77.   DOI
11 Staudinger, J., Zhou, J., Burgess, R., Elledge, S. J. and Olson, E. N. (1995) PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system. J. Cell. Biol. 128, 263-271.   DOI
12 Torres, G. E., Yao, W. D., Mohn, A. R., Quan, H., Kim, K. M., Levey, A. I., Staudinger, J. and Caron, M. G. (2001) Functional interaction between monoamine plasma membrane transporters and the synaptic PDZ domain-containing protein PICK1. Neuron 30, 121-134.   DOI
13 Violin, J. D., Dipilato, L. M., Yildirim, N., Elston, T. C., Zhang, J. and Lefkowitz, R. J. (2008) beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics. J. Biol. Chem. 283, 2949-2961.   DOI
14 Xia, J., Zhang, X., Staudinger, J. and Huganir, R. L. (1999) Clustering of AMPA receptors by the synaptic PDZ domain-containing protein PICK1. Neuron 22, 179-187.   DOI
15 Zheng, M., Cheong, S. Y., Min, C., Jin, M., Cho, D. I. and Kim, K. M. (2011) beta-arrestin2 plays permissive roles in the inhibitory activities of RGS9-2 on G protein-coupled receptors by maintaining RGS9-2 in the open conformation. Mol. Cell Biol. 31, 4887-4901.   DOI
16 Cho, D., Zheng, M., Min, C., Ma, L., Kurose, H., Park, J. H. and Kim, K. M. (2010a) Agonist-induced endocytosis and receptor phosphorylation mediate resensitization of dopamine $D_2$ receptors. Mol. Endocrinol. 24, 574-586.   DOI
17 Beom, S., Cheong, D., Torres, G., Caron, M. G. and Kim, K. M. (2004) Comparative studies of molecular mechanisms of dopamine D2 and D3 receptors for the activation of extracellular signal-regulated kinase. J. Biol. Chem. 279, 28304-28314.   DOI
18 Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P., Salon, J., Christie, M., Machida, C. A., Neve, K. A. and Civelli, O. (1988) Cloning and expression of a rat D2 dopamine receptor cDNA. Nature 336, 783-787.   DOI
19 Cheng, Y. and Prusoff, W. H. (1973) Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099-3108.   DOI
20 Cho, D. I., Zheng, M. and Kim, K. M. (2010b) Current perspectives on the selective regulation of dopamine $D_2$ and $D_3$ receptors. Arch. Pharm. Res. 33, 1521-1538.   DOI
21 Min, C., Zheng, M., Zhang, X., Caron, M. G. and Kim, K. M. (2013) Novel roles for beta-arrestins in the regulation of pharmacological sequestration to predict agonist-induced desensitization of dopamine D3 receptors. Br. J. Pharmacol. 170, 1112-1129.   DOI
22 Lee, F. J., Pei, L., Moszczynska, A., Vukusic, B., Fletcher, P. J. and Liu, F. (2007) Dopamine transporter cell surface localization facilitated by a direct interaction with the dopamine D2 receptor. EMBO J. 26, 2127-2136.   DOI
23 Lohse, M. J., Lefkowitz, R. J., Caron, M. G. and Benovic, J. L. (1989) Inhibition of beta-adrenergic receptor kinase prevents rapid homologous desensitization of beta 2-adrenergic receptors. Proc. Natl. Acad. Sci. U.S.A. 86, 3011-3015.   DOI
24 Mayfield, R. D. and Zahniser, N. R. (2001) Dopamine D2 receptor regulation of the dopamine transporter expressed in Xenopus laevis oocytes is voltage-independent. Mol. Pharmacol. 59, 113-121.   DOI
25 Mostafapour, S., Kobilka, B. K. and Von Zastrow, M. (1996) Pharmacological sequestration of a chimeric beta 3/beta 2 adrenergic receptor occurs without a corresponding amount of receptor internalization. Recept. Signal Transduct. 6, 151-163.
26 Pao, C. S. and Benovic, J. L. (2002) Phosphorylation-independent desensitization of G protein-coupled receptors? Sci. STKE 2002, PE42.
27 Rocca, D. L., Martin, S., Jenkins, E. L. and Hanley, J. G. (2008) Inhibition of Arp2/3-mediated actin polymerization by PICK1 regulates neuronal morphology and AMPA receptor endocytosis. Nat. Cell Biol. 10, 259-271.   DOI
28 Sibley, D. R., Benovic, J. L., Caron, M. G. and Lefkowitz, R. J. (1987) Molecular mechanisms of beta-adrenergic receptor desensitization. Adv. Exp. Med. Biol. 221, 253-273.   DOI