• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2661-2665
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• 2016

Silibinin is a natural polyphenol with high antioxidant and anticancer properties. In this study, its influence on two of the most commonly employed human breast cancer cell lines, MCF-7 and T47D, and one non-malignant MCF-10A cell line, were investigated and compared. Cell viability, the cell cycle distribution and apoptosis induction were analyzed by MTT and flow cytometry, respectively. The effect of silibinin on PTEN, Bcl-2, P21, and P27 mRNAs expression was also investigated by real-time RT-PCR. It was found that silibinin caused G1 cell cycle arrest in MCF-7 and MCF-10A cells but had no effect on the T47D cell cycle. Silibinin induced cytotoxic and apoptotic effects in T47D cells more than the MCF-7 cells and had no cytotoxic effect in MCF-10A cells under the same conditions. Silibinin upregulated PTEN in MCF-7 and caused slightly increased P21 mRNA expression in T47D cells and slightly increased PTEN and P21 expression in MCF-10A cells. Bcl-2 expression decreased in all of the examined cells under silibinin treatment. P27 mRNA expression upregulated in T47D and MCF-10A cells under silibinin treatment. PTEN mRNA in T47D and P21 and P27 mRNAsin MCF-7 were not affected by silibinin. These results suggest that silibinin has mostly different inhibitory effects in breast cancer cells and might be an effective anticancer agent for some cells linked to influence on cell cycle progression.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.343-347
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• 2003

황토볼을 이용하여 폐수처리를 한 결과 다음과 같은 결과를 보였다. 흐름도 A에서는 T-P 0.5ppm이하, T-N 1.0 ppm이하, COD 10ppm 이하였으며, 흐름도 B에서는 T-P 0.3ppm이하, T-N 5.0 ppm이하, COD 15 ppm 이하의 결과를 보여 주었다. 흐름도 C에서는 T-P 0.6ppm이하, T-N 10 ppm이하, COD 15 ppm 이하였으며, 흐름도 D에서는 T-P 1 ppm, T-N 8 ppm이하, COD 20ppm 이하의 결과를 보여 주었다. BOD는 각 흐름도 A, B, C, D에서 COD보다 높은 경우에는 6 ppm, 낮은 경우에는 3 ppm 정도의 차이를 보였다. SS는 각 공정에 따라 그다지 큰 차이를 보이고 있지 않으며, 1.0 처리 용량 Ton/day으로 계산 할 경우에 5 - 20 g/day 정도를 보이고 있다. 이러한 결과치는 하수종말처리장(특별대책지역 및 잠실수중보권지역)의 2 ppm 및 폐수처리시설(농공단지, 오${\cdot}$폐수처리시설 포함)의 T-P 8 ppm, T-N 질소성분 60 ppm이내의 탄소원 COD 40 ppm 이내의 기준에 해당하는 수치의 좋은 결과로 황토볼을 이용한 폐수처리 시스템의 가능성을 보여주고 있다.

• 대한수학회논문집
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• 제27권1호
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• pp.77-95
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• 2012

For a bounded linear operator T we prove the following assertions: (a) If T is algebraically (p, k)-quasihyponormal, then T is a-isoloid, polaroid, reguloid and a-polaroid. (b) If $T^*$ is algebraically (p, k)-quasihyponormal, then a-Weyl's theorem holds for f(T) for every $f{\in}Hol({\sigma}T))$, where $Hol({\sigma}(T))$ is the space of all functions that analytic in an open neighborhoods of ${\sigma}(T)$ of T. (c) If $T^*$ is algebraically (p, k)-quasihyponormal, then generalized a-Weyl's theorem holds for f(T) for every $f{\in}Hol({\sigma}T))$. (d) If T is a (p, k)-quasihyponormal operator, then the spectral mapping theorem holds for semi-B-essential approximate point spectrum $\sigma_{SBF_+^-}(T)$, and for left Drazin spectrum ${\sigma}_{lD}(T)$ for every $f{\in}Hol({\sigma}T))$.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1569-1576
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• 2013

The study was conducted to evaluate the effect of vitamin E and zinc supplementation on energy metabolites, lipid peroxidation, and milk production in peripartum Sahiwal cows. For this, thirty-two pregnant dry Sahiwal cows were selected at sixty days prepartum and divided into four groups viz control, $T_1$, $T_2$, and $T_3$ of eight each. Group $T_1$ were supplemented with zinc at 60 ppm/d/cow, group $T_2$ were supplemented with vitamin E at 1,000 IU/d/cow and group $T_3$ were supplemented with combination of vitamin E at 1,000 IU/d/cow and zinc at 60 ppm/d/cow during d 60 prepartum to d 90 postpartum. Blood samples were collected on d -60, -45, -30, -15, -7, -3, 0, 3, 7, 15, 30, 45, 60, 90, and 120 with respect to day of parturition and analysed for glucose, non esterified fatty acid, and thiobarbituric acid reactive substance. Body condition score was maintained significantly better (p<0.05) in $T_3$ than in the control, $T_1$ and $T_2$ groups. Overall glucose level was higher (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. Levels of nonesterified fatty acid, and thiobarbituric acid reactive substance were lower (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. Milk yield was higher (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. In conclusion, the present study indicated that the supplementation of vitamin E and zinc in peripartum Sahiwal cows enhanced milk production by reducing negative energy balance.

• International Journal of Internet, Broadcasting and Communication
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• 제6권2호
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• pp.13-16
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• 2014

This study aims to examine the effects of watching 2D and 3D images on the blink rate. Regarding the image watch, their blink rate for 2D and 3D images was separately checked for 1 minute in the 1m distance, before the watch, after 15 minutes of watch, and after 30 minutes of watch. About the change of their blink rate in the 2D image watch, it tended to become higher than that before watching the image; however, there was no statistical significance (paired t-test, p=0.106, p=0.062). And in the 2D image watch, it tended to increase in comparison between after 15 minutes and after 30 minutes, but there was no statistical significance (paired t-test, p=0.623). Meanwhile, about the change of their blink rate in the 3D image watch, it tended to decrease statistically significantly both after 15 minutes and after 30 minutes when compared with that before watching the image (paired t-test, p=0.000, p=0.000). In the 3D image watch, it tended to increase in comparison between after 15 minutes and after 30 minutes; however, there was no statistical significance (paired t-test, p=0.867).

• Journal of Chest Surgery
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• 제14권3호
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• pp.235-240
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• 1981

A total of 102 patients who had an Open Heart Surgery from April 1976 to July 1981 were reviewed. 55 paeitnts were congenital heart disease and 47 patients were acquired heart disease. Among SS patients of congenital heart disease, 18 T 0 F, 18 V S D, 8 A S D, and each one case of l\ulcorner 0 R V, Truncus arteriosus, Ebstein anomaly, Single ventricle, P D A, P 5, A S D + P 5, E C D, V 5 D + P D A, A - P window, D C R V were noted respectively. In 47 patients of acquired heart disease and one Ebstein patient, 46 prosthetic values were implanted: 17 had M V R, 4 had A V R, 2 had M V R + A V R, and 4 had M V R + T V R and one T V R. The operative mortality was 8.S% in acquired heart disease and 17% in congenital heart disease. The follow up period was between 6 months and 6 years. There were 3 cases of late mortality in acquired heart disease and one case in congenital heart disease.

• 아시안잔디학회지
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• 제12권3호
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• pp.173-182
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• 1998

골프장 그린에 이용되는 creeping bentgrass 'Penncross'의 여름철 그린의 질을 높이기 위하여 식물생장억제제인 chlormequat chloride(C) 250, 500, 1000배액, daminozide(D) 50, 100, 200배액, paclobutrazol(P) 1000, 2000, 4000배액, trinexpac-ethyl(T) 1000, 2000, 4000배액 그리고 uniconazole(U) 25, 50, 100배액을 엽면 처리하여 생장과 품질을 조사하였다. 엽장과 엽폭, 그리고 포복경의 절간길이는 대조구에 비해 모든 처리구에서 억제되었는데 엽장은 D-50에서 포복경의 절간길이는 T-2000에서 가장 효과적이었다. 잎두께는 C-250, D-50, T-2000과 T-4000에서 가장 좋았으며 단위면적당 직립경수로 조사한 직립경의 밀도 증가는 P-2000과 4000에서 가장 효과적이었으나 전체적으로 볼 때 대조구에 비해 15∼20％ 정도 증가하였다. 전체 건물중은 P-2000과 4000에서 증가된 반면 잎 건물중은 T-4000, U-25와 50에서 높게 나타났고 나머지 처리구에서는 대조구와 별다른 차이를 나타내지 않았다. 잔디의 품질을 평가하기 위한 시각적 평가 결과 P, T 그리고 U에서는 황변현상이 나타나 녹색유지에는 적합치 않은 것으로 나타났다. 이상을 종합해보면 C-250, D-50, 그리고 T-4000은 실용화 가능성이 있는 것으로 고려되며 여름철 골프장 그린관리를 위해 식물생장억제제의 적용은 크리핑 벤트그라스의 직립경의 밀도를 증가시키고 잔디깎기 회수를 줄이며 여름철 그린의 품질을 향상시킬 수 있을 것으로 사료된다.

• 약학회지
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• 제39권1호
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• pp.78-84
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• 1995

Based on Computer graphics molecular modeling method, quinolone derivatives as DNA-gyrase inhibitors formed stable DNA-intercalation complex with deoxycytidilyl-3',5'-deoxy guanosine[d($C_{p}G)_{2}$] dinucleotide. When d($C_{p}G)_{2}$ and d($A_{p}T)_{2}$, were compared in order to find out which DNA could form more stable DNA-Drug complex based on interaction energy($\Delta$E) and DNA-Drug complex energy, d($C_{p}G)_{2}$ resulted in lower energy than d($A_{p}T)_{2}$.

• Clinical and Experimental Reproductive Medicine
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• 제50권4호
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• pp.292-298
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• 2023

Objective: Ovarian torsion is a gynecological disorder that causes ischemia-reperfusion injuries in the ovary. Our study investigated berberine's short- and long-term effects on ovarian ischemia-reperfusion injuries. Methods: This study included 28 Wistar albino female rats weighing 180 to 220 g, which were divided into four groups: sham (S), torsion/detorsion (T/D), torsion/ detorsion+single dose berberine (T/D+Bb), and torsion/detorsion+15 days berberine (T/D+15Bb). The torsion and detorsion model was applied in all non-sham groups. In the T/D+Bb group, a single dose of berberine was administered, while in the T/D+15Bb group, berberine was administered over a period of 15 days. After the rats were euthanized, their ovaries were excised. The left ovaries were used for histopathologic evaluation, which included ovarian injury scoring and follicle count, while the right ovaries were used for biochemical analyses (tissue transforming growth factor-β [TGF-β] and alpha-smooth muscle actin [α-SMA] levels). Results: The histopathologic evaluation scores for the ovaries were significantly lower in the T/D+B group (p<0.05) and the T/D+15B group (p<0.005) than in the T/D group. The follicle counts in the T/D group were lower than those in both the sham and treated groups (p<0.005). The TGF-β levels were significantly lower in the T/D+15B group (p<0.005), whereas the α-SMA levels did not show a significant difference. Conclusion: Both short- and long-term berberine use could potentially have therapeutic effects on ovarian torsion. Long-term berberine use exhibited anti-inflammatory effects by reducing TGF-β levels, thereby preventing ischemia-reperfusion injuries. Therefore, we suggest that long-term berberine use could be beneficial for ovarian torsion.

• Journal of Korean Neurosurgical Society
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• 제66권2호
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• pp.144-154
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• 2023

Objective : Stroke caused from large vessel occlusion (LVO) has emerged as the most common stroke subtype worldwide. Intravenous tissue plasminogen activator administration (IV-tPA) and additional intraarterial thrombectomy (IA-Tx) is regarded as standard treatment. In this study, the authors try to find the early recanalization rate of IV-tPA in LVO stroke patients. Methods : Total 300 patients undertook IA-Tx with confirmed anterior circulation LVO, were analyzed retrospectively. Brain computed tomography angiography (CTA) was the initial imaging study and acute stroke magnetic resonance angiography (MRA) followed after finished IV-tPA. Early recanalization rate was evaluated by acute stroke MRA within 2 hours after the IV-tPA. In 167 patients undertook IV-tPA only and 133 non-recanalized patients by IV-tPA, additional IA-Tx tried (IV-tPA + IA-Tx group). And 131 patients, non-recanalized by IV-tPA (IV-tPA group) additional IA-Tx recommend and tried according to the patient condition and compliance. Results : Early recanalization rate of LVO after IV-tPA was 12.0% (36/300). In recanalized patients, favorable outcome (modified Rankin Scale, 0-2) was 69.4% (25/36) while it was 32.1% (42/131, p<0.001) in non-recanalized patients. Among 133 patients, non-recanalized after intravenous recombinant tissue plasminogen activator and undertook additional IA-Tx, the clinical outcome was better than not undertaken additional IA-Tx (favorable outcome was 42.9% vs. 32.1%, p=0.046). Analysis according to the perfusion/diffusion (P/D)-mismatching or not, in patient with IV-tPA with IA-Tx (133 patients), favorable outcome was higher in P/D-mismatching patient (52/104; 50.0%) than P/D-matching patients (5/29; 17.2%; p=0.001). Which treatment tired, P/D-mismatching was favored in clinical outcome (iv-tPA only, p=0.008 and IV-tPA with IA-Tx, p=0.001). Conclusion : The P/D-mismatching influences on the recanalization and clinical outcomes of IV-tPA and IA-Tx. The authors would like to propose that we had better prepare IA-Tx when LVO is diagnosed on initial diagnostic imaging. Furthermore, if the patient shows P/D-mismatching on MRA after IV-tPA, additional IA-Tx improves treatment results and lessen the futile recanalization.