• Korean Journal of Microbiology
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• v.28 no.2
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• pp.169-173
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• 1990

For the overproduction of L-phenylalanine using Escherichia coli, the authors constructed various recombinant plasmids including pMW 10, pMW 11 and pMW 12. The $aroF{FR}$ and $pheA^{FR}$ genes for the production of L-phenylalanine were isolated from Escherichia coli MWEC 101-5 strains. The productivity and atability of Escherichia coli regulatory mutants containing recombinant plasmids were investigated to evaluate the efficiency of the $aroF^{FR}$ and $pheA^{FR}$ genes. The MWEC 101-5/pMW 11 strain produced 24.3g/l of L-phenylalanine while its stability was 73.8 percent. The specific activity of prephenate dehydratase in the MWEC 101-5/pMW 11 strain increased by 26-fold compared with that of Escherichia coli K-12.

• Proceedings of the KIEE Conference
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• 1987.07a
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• pp.424-428
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• 1987

A $2{\mu}m$ CMOS P-well double metal technology has been developed. Phosphorus deep implantation and drive-in diffusion steps were utilized to prevent the low voltage bulk punch through in the short channel, 1.6[${\mu}m$] Leff, PMOS device. Double metal process with the rules of 5[${\mu}m$] 1st metal pitch and 7[${\mu}m$] 2nd metal pitch was successfully implemented by using VLTO, low temperature oxide, as on intermetal dielectric.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.9
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• pp.2331-2338
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• 1994

Experimental conditions of the optical shadow methods of caustics for measurement of the stress intensity factor and the J-integral in various materials(polycarbonate, PMMA, Al 5586D) are investigated. The necessary experimental requirements toe determine accurate values of the stress intensity factors and the J-integrals are described. The ratio of $r_o$ (radius of initial curve) to $r_p$ (plastic zone size) is selected as a parameter to verify the experimental limitation of the method of caustics in determination of fracture parameters. In this study, transmission caustics method was used for compact tension specimens made of polycarbonate and PMMA. while reflection caustics method was applied to c-shaped tension specimen made of Al 5586D. The appropriate ranges of $r_o/r_p$ tp determine accurate values of stress intensity factors were found to be 1.5~1.8. Existing experimental results have been obtained mainly by changing $r_p$ with different loads in $r_o/r_p$. However, in this study we could obtain varying $K_{caus}/K_{th}$ over the wide range of $r_o/r_p$ at fixed load conditions with newly designed optical arrangement. Thus, we could find the range in which theoretical and experimental results agree well each other by changing $r_o$ values only. In Al 5586D specimen, experimental caustics were located inside of the plastic zone, and $K_{caus}/K_{th}$ were found to be not unity in this range. It is found that $J_{caus}/J_{th}=1{\;}with{\;}r_o/t{\geq}0.8$ and the experimental plastic zone includes the contours of caustics.

• BMB Reports
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• v.39 no.1
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• pp.68-75
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• 2006

In higher plants, P450s participate in the biosynthesis of many important secondary metabolites. Here we reported for the first time the isolation of a new cytochrome P450 cDNA that expressed in a stem-specific manner from Camptotheca acuminata (designated as CaSS), a native medicinal plant species in China, using RACE-PCR. The full-length cDNA of CaSS was 1735 bp long containing a 1530 bp open reading frame (ORF) encoding a polypeptide of 509 amino acids. Bioinformatic analysis revealed that CASS contained a heme-binding domain PFGXGRRXCX and showed homology to other plant cytochrome P450 monooxygenases and hydroxylases. Southern blotting analysis revealed that there was only one copy of the CaSS present in the genome of Camptotheca acuminata. Northern blotting analysis revealed that CaSS expressed, in a tissue-specific manner, highly in stem and lowly in root, leaf and flower. Our study suggests that CaSS is likely to be involved in the phenylpropanoid pathway.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.4
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• pp.383-390
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• 2014

The following conclusion was made from the 2D-QSAR model for the tyrosinase inhibitory activity according to the variation of the substituents R1 and R2 in analogues of compound 2-[(2,6-dioxocyclohexyl)methyl]cyclohexane- 1,3-dione (1-23). The best optimized 2D-QSAR model was $Obs.pI_{50}=-0.295({\pm}0.031)TDM$ $-0.120({\pm}0.014)DMZ+0.135({\pm}0.050)DMX.R_2+6.382({\pm}0.17)$, and the correlation $r^2=0.905$) of which was greater than its predictability ($q^2=0.843$). The magnitude of the effect of tyrosinase inhibitory activities was in order of TDM > $DMX.R_2{\geq}DMZ$, and it tended to increase as the hydrophobicity of substrate molecule (ClogP > 0) as well as the steric favor of substituent $R_1$ increased. The analysis of the model implies that inhibitory activity of substrate molecule will increase as $DMX.R_2$ (Dipole moment X component of $R_2$-substituent) increases, while TDM (Total Dipole Moment) and DMZ(Dipole Moment of Z-Component) decrease. As such, it is deemed feasible to conclude, that in order to increase the inhibitory effect, it would be rather desirable to replace the polar groups within the molecules with non-polar functional groups.

• Korean Journal of Ecology and Environment
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• v.40 no.3
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• pp.439-448
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• 2007

The purpose of this study was to investigate the seasonal variations of sediment oxygen demand (SOD) in Lake Paldang and Lake Chungju of the Han River system and to suggest SOD values as parameters for the water quality prediction models of two lakes. SOD was measured at laboratory using sediment collected at 2 sites in Lake Paldang from June to November and at 4 sites in Lake Chungju from May to November in 2005, respectively. It was found from the laboratory test that the SOD in Lake Paldang ranged from 337.8 to 881.0 mg $O_2m^{-2}d^{-1}$ and in Lake Chungju ranged from 143.0 to 969.1 mg $O_2m^{-2}d^{-1}$. The SOD of Lake Paldang showed similar variations to the content of organic matter of sediment. The SOD of Lake Chungju was positively correlated with temperature (r=0.78, p<0.01), $PO_4-P(r=0.79,\;p<0.01)$, TP (r=0.55, p<0.05), DTP (r=0.55, p<0.05), $NO_3-N$ (r=(0.72, p<0.01) of hypolimnetic water. These results indicate that the SOD of Lake Paldang was affected by the content and origin of organic matter of sediment and the SOD of Lake Chungju was closely correlated with physical and chemical factors.

• Journal of radiological science and technology
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• v.25 no.1
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• pp.63-71
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• 2002

The aim of this study Is to develop a simple and fast method which computes in-vivo doses from transmission doses measured doting patient treatment using an ionization chamber. Energy fluence and the dose that reach the chamber positioned behind the patient is modified by three factors: patient attenuation, inverse square attenuation. and scattering. We adopted a straightforward empirical approach using a phantom transmission factor (PTF) which accounts for the contribution from all three factors. It was done as follows. First of all, the phantom transmission factor was measured as a simple ratio of the chamber reading measured with and without a homogeneous phantom in the radiation beam according to various field sizes($r_p$), phantom to chamber distance($d_g$) and phantom thickness($T_p$). Secondly, we used the concept of effective field to the cases with inhomogeneous phantom (patients) and irregular fields. The effective field size is calculated by finding the field size that produces the same value of PTF to that for the irregular field and/or inhomogeneous phantom. The hypothesis is that the presence of inhomogeneity and irregular field can be accommodated to a certain extent by altering the field size. Thirdly, the center dose at the prescription depth can be computed using the new TMR($r_{p,eff}$) and Sp($r_{p,eff}$) from the effective field size. After that, when TMR(d, $r_{p,eff}$) and SP($r_{p,eff}$) are acquired. the tumor dose is as follows. $$D_{center}=D_t/PTF(d_g,\;T_p){\times}(\frac{SCD}{SAD})^2{\times}BSF(r_o){\times}S_p(r_{p,eff}){\times}TMR(d,\;r_{p,eff})$$ To make certain the accuracy of this method, we checked the accuracy for the following four cases; in cases of regular or irregular field size, inhomogeneous material included, any errors made and clinical situation. The errors were within 2.3% for regular field size, 3.0% irregular field size, 2.4% when inhomogeneous material was included in the phantom, 3.8% for 6 MV when the error was made purposely, 4.7% for 10 MV and 1.8% for the measurement of a patient in clinic. It is considered that this methode can make the quality control for dose at the time of radiation therapy because it is non-invasive that makes possible to measure the doses whenever a patient is given a therapy as well as eliminates the problem for entrance or exit dose measurement.

• Korean Management Science Review
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• v.34 no.2
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• pp.67-83
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• 2017

This paper proposes a R&D project performance measurement model for private firms combining balanced scorecard (BSC) and data envelopment analysis (DEA). The efficiency of R&D projects is measured in terms of each of the three perspectives of BSC by using DEA : the internal process perspective (DEA-P), the customer perspective (DEA-C), and the financial perspective (DEA-F). The performance indicators of the three perspectives of BSC are considered as outputs of the corresponding DEA models. To provide strategic implications for R&D planning, we also propose the R&D project performance matrices composed of two different types of efficiency dimensions. The proposed model is expected to be fruitfully utilized for R&D performance measurement of private firms.

• Bulletin of the Korean Mathematical Society
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• v.61 no.4
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• pp.959-968
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• 2024

Suppose f belongs to the Bloch space with f(0) = 0. For 0 < r < 1 and 0 < p < ∞, we show that $$M_p(r,\,f)\,=\,({\frac{1}{2\pi}}{\int_{0}^{2\pi}}\,{\mid}f(re^{it}){\mid}^pdt)^{1/p}\,{\leq}\,({\frac{{\Gamma}(\frac{p}{2}+1)}{{\Gamma}(\frac{p}{2}+1-k)}})^{1/p}\,{\rho}{\mathcal{B}}(log\frac{1}{1-r^2})^{1/2},$$ where ρʙ(f) = sup_z∈ⅅ(1 - |z|²)|f'(z)| and k is the integer satisfying 0 < p - 2k ≤ 2. Moreover, we prove that for 0 < r < 1 and p > 1, $${\parallel}f_r{\parallel}_{B_q}\,{\leq}\,r\,{\rho}{\mathcal{B}}(f)(\frac{1}{(1-r^2)(q-1)})^{1/q},$$ where f_r(z) = f(rz) and ||·||ʙ_q is the Besov seminorm given by ║f║ʙ_q = (∫_𝔻 |f'(z)|^q(1-|z|²)^q-2dA(z)). These results improve previous results of Clunie and MacGregor.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.110-117
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• 2008

Three kinds of prenylated flavonols, icariside I, icariside II, and icaritin, were isolated from an icariin hydrolysate and their effects on melanogenesis evaluated based on mushroom tyrosinase inhibition and quantifying the melanin contents in melanocytes. Although none of the compounds had an effect on tyrosinase activity, icariside II and icaritin both effectively inhibited the melanin contents with an $IC_{50}$ of 10.53 and $11.13{\mu}M$, respectively. Whereas icariside II was obtained from a reaction with ${\beta}$-glucosidase and cellulase, the icariin was not completely converted into icariside II. Thus, for the high-purity production of icariside II, the reaction was optimized using the response surface methodology, where an enzyme concentration of 5.0mg/ml, pH 7, $37.5^{\circ}C$, and 8 h reaction time were selected as the central conditions for the central composite design (CCD) for the enzymatic hydrolysis of icariin into icariside II using cellulase. Empirical models were developed to describe the relationships between the operating factors and the response (icariside II yield). A statistical analysis indicated that all four factors had a significant effect (p<0.01) on the icariside II production. The coefficient of determination $(R^2)$ was good for the model (0.9853), and the optimum production conditions for icariside II was an enzyme concentration of 7.5mg/ml, pH 5, $50^{\circ}C$, and 12 h reaction time. A good agreement between the predicted and experimental data under the designed optimal conditions confirmed the usefulness of the model. A laboratory pilot scale was also successful.