• Journal of Animal Science and Technology
• /
• 제46권4호
• /
• pp.701-706
• /
• 2004

The objective of this experiment was to compare the dry matter intake, nutrients digestibility and nitrogen and energy utilization of herbages harvested from turf type mixtures {tall fescue(Rebell Jr.) 40%+ perennial ryegrass(Palmer II) 20%+ Kentucky bluegrass(Newport) 10%+ redtop(Banicuda) 10%+ red fescue(Salem) 10%+ creeping bentgrass(Crenshaw) 10%} and tall type mixtures {orchardgrass(Potomac) 40%+ tall fescue(Fawn) 20%+ Festuloium braunii(Paulita) 10%+ perennial ryegrass(Reveille) 10%+ timothy(Climax) 10%+ red clover(Kenblue) 10%+ alfalfa(Vernal) 10%} by Korean native goats. This experiment was conducted by total collection method in laboratory, 2001. The voluntary DM intake per body weight were slightly higher for turf type mixtures(28.9g) than that of tall type mixtures(26.2g), but there was no significant difference. The digestibility of cellular constituents was slightly higher for turf type mixtures than that of tall type mixtures(p<0.05), but digestibility of DM, NDF and ADF did not differ between two mixtures. The retained nitrogen percent(apparently biological value) was slightly higher for turf type mixtures(56.7%) than that of tall type mixtures(56.4%), but there was no significant difference between two mixtures. Apparently retained digestible energy was slightly higher for turf type mixtures(58.3%) than that of tall type mixtures(57.9%), but there was no significant difference between two mixtures. Based on the results, the herbage utilization by Korean native goats did not showed any significant difference between two mixtures, therefore there is a possibility that herbages of turf type mixtures can be used as a roughages by livestock.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 한국방사성폐기물학회 2005년도 춘계 학술대회
• /
• pp.161-166
• /
• 2005

In KAERI, about 3,100 drums containing soil have been stored. The soils were generated from the decommissioning process of Seoul office in 1988. Those soils occupy about $27\%$ of the capacity of the radioactive waste storage facility and make it difficult to maintain the storage facility. The major radioactive nuclides contained in the soils were expected to be Co-60 and Cs-137. As 16 years have passed, the radioactivity of those nuclides have decayed a lot. In this study, as a basis of regulatory clearance, radionuclides and radioactivity concentration of soils were analyzed. As a result, there are only Co-60 and Cs-137 in soils as ${\gamma}-emitters$. The total concentration of ${\gamma}-emitters$ in soil is analyzed as about $0.01\;{\sim}\;0.12$ Bq/g. As the soils are expected to be regulatory cleared in 2009, those concentrations will decay to be less than 0.1 Bq/g. This concentration can be meet the regulatory criteria suggested by IAEA. The regulatory clearance will be proceeded based on not only the assessment results of environmental influence but also related regulations.

• Applied Biological Chemistry
• /
• 제37권2호
• /
• pp.100-104
• /
• 1994

From the 48 hour-cultured mycelial broth of Phellinus linteus, six compounds were isolated by means of ethyl acetate extraction, silica gel column chromatography and preparative thin layer chromatography, consecutively. Compound 1 was identified as a succinic acid by the comparison of its spectral data with authentic sample. Compounds 2 and 3 were identified as p-hydroxyphenyl acetic acid methyl ester and p-hydroxybenzaldehyde by spectroscopic studies, respectively. NMR and MS studies of compound 6 revealed that it was 2,5-dihydroxymethyl furan. Compound 4, which showed similar NMR absorptions and MS fragmentation pattern with those of compound 6 was identified as 2-hydroxymethyl-5-methoxymethylfuran. These structures were verified by the spectral data of the acetate derivatives of the compounds. Compound 5 was supposed to be a N-acetyltyramine from its $^1H-NMR$ and EI-MS data, and its structure was confirmed by a synthesis starting from tyramine.

• Applied Biological Chemistry
• /
• 제44권1호
• /
• pp.12-19
• /
• 2001

The major volatiles from Allium species were found to be sulfide compounds and the ratio of sulfide to volatiles was 66.1% in garlic, 66.1% in scallion, 62.3% in green onion, 39.2% in onion, and 4.2% in chive. Trace of cyclooctasulfur was found to be present among the volatiles. The most oviposition of 17.2% occurred at diallyl sulfide and the least of 0.8% at acetylthiophene whereas the most oviposition of 43.3% occurred at ethyl alcohol if concentration was 100%. Among the organic solvents used for dilution, ethyl alcohol received the highest 52.5% of oviposition and ether the lowest of 5.9%. Furfuryl mercaptan which is also one of the volatiles, received 46.9% of oviposition. For oviposition site, D. antiqua preferred sulfides at near 1%, ethyl alcohol at high, and other volatiles at various concentrations. At 100% concentration, most volatiles except dimethyl disulfide and ethyl alcohol received less oviposition than the control which was watered sand with no volatiles added.

• Applied Microscopy
• /
• 제15권1호
• /
• pp.31-58
• /
• 1985

The morphological study on different types of cells of reproductive organ including spermatogenesis in the adult planaria was performed to observe their cytochemical and ultrastructural characteristics. 1. Spermatogenesis The circular luminated material appears immediately inside the nuclear envelope of early spermatid and is found also in the nucleus of sperm, but typical acrosomal structures cannot be observed. Approximately ten of small-sized mitochondria occur around the nucleus in the transitional phase from primary spermatocyte to secondary spermatocyte, but in sperm a long mitochondrion is closely associated with nucleus, parellel to long axis of it. The sperm has a relatively long head connected with two tails via hollow neck. 2. Reproductive organ The penis bulb and the bursa stalk were observed. (1) Penis bulb The cells constituted penis bulb are classified into six types on the basis of ultrastructure of the cells and cytochemistry of the cytoplasmic granules. 1) A-type cells: These cells exhibiting low electron density are mainly occupied by large nucleus. These cells possess two different types of granules: highly electron-dense round granules with an average size of $0.9{\mu}m$, and electron-dense granules exhibit PAS-positive reaction. 2) B-type cells contain PAS-positive granules with the size of about $0.4{\mu}m$. They are rich in free ribosomes and mitochondria. 3) C-type cells are found to be dark cells due to high electron-density. These cells are largely occupied by large nucleus. 4) D-type cells: These cells are seen as light cells which have poorly developed cell organelles. 5) E-type tells: These cells contain a large number of glycogen granules which occupy most of cell. 6) F-type cells: These arc parietal epidermal cells surrounding the genital antrum. These cells are characterized by their finger-like shapes and the presence of a number of electron-dense, irregularly-shaped structures inside cells. The relatively large electron-lucent granules can be also found. The F-type cells possess numerous microvilli on their free surfaces. (2) Bursa stalk The cells constituted bursa stalk are classified into 3 types on the basis of cell shapes and presences of electron-dense or electron-lucent granules. 7) G-type cells with a long cytoplasmic process. They have large nuclei and poorly developed cell organelles. 8) H-type cells: These cells are characterized by the presence of a long cytoplasmic process and relatively highly electron-dense cytoplasmic profile. They have poorly developed cell organelles. 9) I-type cells contain large electron-lucent granules which exhibit negative reactions with three kinds of cytochemical staining methods used in this experiment. The fine electron-dense structures can be found inside these granules.

• Journal of The Korean Society of Grassland and Forage Science
• /
• 제35권3호
• /
• pp.257-263
• /
• 2015

This study was conducted to assess the potential of using NIRS to accurately determine the chemical composition and fermentation parameters in fresh coarse sorghum and sudangrass silage. Near Infrared Spectroscopy (NIRS) has been increasingly used as a rapid and accurate method to analyze the quality of cereals and dried animal forage. However, silage analysis by NIRS has a limitation in analyzing dried and ground samples in farm-scale applications because the fermentative products are lost during the drying process. Fresh coarse silage samples were scanned at 1 nm intervals over the wavelength range of 680~2500 nm, and the optical data were obtained as log 1/Reflectance (log 1/R). The spectral data were regressed, using partial least squares (PLS) multivariate analysis in conjunction with first and second order derivatization, with a scatter correction procedure (standard normal variate and detrend (SNV&D)) to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The results of this study showed that NIRS predicted the chemical constituents with a high degree of accuracy (i.e. the correlation coefficient of cross validation ($R^2{_{cv}}$) ranged from 0.86~0.96), except for crude ash which had an $R^2{_{cv}}$ of 0.68. Comparison of the mathematical treatments for raw spectra showed that the second-order derivatization procedure produced the best result for all the treatments, except for neutral detergent fiber (NDF). The best mathematical treatment for moisture, acid detergent fiber (ADF), crude protein (CP) and pH was 2,16,16 respectively while the best mathematical treatment for crude ash, lactic acid and total acid was 2,8,8 respectively. The calibrations of fermentation products produced poorer calibrations (RPD < 2.5) with acetic and butyric acid. The pH, lactic acid and total acids were predicted with considerable accuracy at $R^2{_{cv}}$ 0.72~0.77. This study indicated that NIRS calibrations based on fresh coarse sorghum and sudangrass silage spectra have the capability of assessing the forage quality control

• Journal of the Korea Academia-Industrial cooperation Society
• /
• 제16권4호
• /
• pp.2647-2655
• /
• 2015

This study has the purpose to evaluate the effects of cooperative learning applying Jigsaw model in simulation based infection control education by comparing perception of infection control, intrinsic motive and learning satisfaction, 54 first-year health related students. It is composed of 27 students of the experimental group and 27 students of the control group. In order to evaluate the homogeneity between the two groups, it surveyed in advance perception of infection control, intrinsic motive and learning satisfaction. There was no significant difference between the two groups, The education program composed cooperative learning applying Jigsaw Model, simulation practice and debriefing to the experimental group was performed twice for 2 weeks and lecture and skill training, simulation practice and debriefing were performed to the control group. After two-week education, perception of infection control, intrinsic motive and learning satisfaction of the participants the research were surveyed. As a result of this study, the Jigsow Model indicated the perception of infection control and learning satisfaction experimental group was significantly high in statistic. Based on this research result, I suggests that utilization of cooperative learning applying the Jigsaw Model as a strategy to improve the scholatic achievement and learning satisfaction of students in the variable simulation based education.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• 제10권11호
• /
• pp.3121-3129
• /
• 2009

Conventional marine radar systems utilize pulse radar which is capable of high-power transmissions and is effective for remote detection purposes. A pulse radar is most commonly used on medium or large vessels due to its expensive installation and maintenance costs. I propose the use of a Frequency Modulated Continuous Wave (FMCW) radar system operated at low-power and high-resolution instead of the conventional pulse-radar based system. The transmitted and received signals of the FMCW radar system were theoretically analyzed and radar signal processing design and simulation experiments were performed to detect the range and speed. Intermediate Frequency (IF) signal mixed with virtual transmit and receive signals were generated to perform FMCW radar signal processing simulations where the IF signal underwent noise reduction through a lowpass filter. The maximum frequency was derived through the sample interval of the FFT size instead of using A/D converter. This maximum frequency was used to get the frequency range and frequency speed which were in turn used to calculate the range and speed. The virtual beat frequency generated using MATLAB is utilized to analyze the beat frequency used in the actual FMCW radar system signal processing. The differences in the range and speed of the beat frequency signals are processed and analyzed.

• Journal of Korean Society for Atmospheric Environment
• /
• 제22권5호
• /
• pp.590-603
• /
• 2006

Size-and time-resolved aerosol samples were collected using an eight-stage Davis rotating unit for monitoring (DRUM) sampler from 23 March to 29 April 2001 at Gosan, Jeju Island, Korea, which is one of the super sites of Asia-Pacific Regional Aerosol Characterization Experiment(ACE-Asia). These samples were analyzed using synchrotron X-ray fluorescence for 3-hr average concentrations of 19 elements including Al, Si, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Br, Rb, and Pb. The size-resolved data sets were then analyzed using the positive matrix factorization(PMF) technique to identify possible sources and estimate their contributions to particulate matter mass. PMF analysis uses the uncertainty of the measured data to provide an optimal weighting. Twelve sources were resolved in eight size ranges($0.09{\sim}12{\mu}m$) and included continental soil, local soil, sea salt, biomass/biofuel burning, coal combustion, oil combustion, municipal incineration, nonferrous metal source, ferrous metal source, gasoline vehicle, diesel vehicle, and volcanic emission. The PMF result of size-resolved source contributions showed that natural sources represented by local soil, sea salt, continental soil, and volcanic emission contributed about 79% to the predicted primary particulate matter(PM) mass in the coarse size range ($1.15{\sim}12{\mu}m$) while anthropogenic sources such as coal combustion and biomass/biofuel burning contributed about 58% in the fine size range($0.56{\sim}2.5{\mu}m$). The diesel vehicle source contributed mostly in ultra-fine size range($0.09{\sim}0.56{\mu}m$) and was responsible for about 56% of the primary PM mass.

• Microbiology and Biotechnology Letters
• /
• 제20권5호
• /
• pp.574-582
• /
• 1992

Exo-xylanase encoded by the xylA gene of Bacillus stearothermoPhillus was produced from Escherichia coli ]M109 carrying a recombinant plasmid pMGL Synthesis of the enzyme was observed to be cell-associated, and about 94% of the enzyme synthesized was located in the cytoplasmic region. The maximum production was attained when the E. coli strain was grown at $37^{\circ}C$ for 8 hours on the medium containing 0.5% fructose, 1.0% tryptone, 1.0% sodium chloride, and 0.5% yeast extract. The exo-xylanase was purified to homogeneity using a combination of salting out with ammonium sulfate, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-IOO gel filtration, and Sephadex G-150 gel filtration. The' purified enzyme was most active at pH 6.0 and $45^{\circ}C$. $Ca^{2+}$ and $Co^{2+}$ activated the exo-xylanase activity by about 20% while $Ag^{2+}$, $Fe^{2+}$, $Mg^{2+}$ and $Zn^{2+}$ inhibited the enzyme activity by up to 60%. The $K_m$, value on p-nitrophenyl-$\beta$-D-xylanopyranoside was 2.75 mM. The enzyme had a pI value of 4.7. The estimated molecular weight of the native protein was 200,000 daL SDS-polyacrylamide gel electrophoresis analysis suggested that the native enzyme was a trimer composed of three identical 66,000 da!. polypeptides. The purified enzyme efficiently converted all the xylo-oligosaccharides tested to xylose. It was also confirmed that the enzyme split xylans in an exo-manner even though the degree of hydrolysis was fairly low. The xylanolytic enzyme was, therefore, classified to be one of the few bacterial exo-xylanases lacking transferase activity.