• Journal of Animal Science and Technology
• /
• v.63 no.6
• /
• pp.1314-1327
• /
• 2021

Bacillus is characterized by the formation of spores in harsh environments, which makes it suitable for use as a probiotic for feed because of thermostability and high survival rate, even under long-term storage. This study was conducted to investigate the effects of Bacillus-based probiotics on growth performance, nutrient digestibility, intestinal morphology, immune response, and intestinal microbiota of weaned pigs. A total of 40 weaned pigs (7.01 ± 0.86 kg body weight [BW]; 28 d old) were randomly assigned to two treatments (4 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW and sex). The dietary treatment was either a typical nursery diet based on corn and soybean meal (CON) or CON supplemented with 0.01% probiotics containing a mixture of Bacillus subtilis and Bacillus licheniformis (PRO). Fecal samples were collected daily by rectal palpation for the last 3 days after a 4-day adaptation. Blood, ileal digesta, and intestinal tissue samples were collected from one pig in each pen at the respective time points. The PRO group did not affect the feed efficiency, but the average daily gain was significantly improved (p < 0.05). The PRO group showed a trend of improved crude protein digestibility (p < 0.10). The serum transforming growth factor-β1 level tended to be higher (p < 0.10) in the PRO group on days 7 and 14. There was no difference in phylum level of the intestinal microbiota, but there were differences in genus composition and proportions. However, β-diversity analysis showed no statistical differences between the CON and the PRO groups. Taken together, Bacillus-based probiotics had beneficial effects on the growth performance, immune system, and intestinal microbiota of weaned pigs, suggesting that Bacillus can be utilized as a functional probiotic for weaned pigs.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.4
• /
• pp.56-66
• /
• 2021

In this study, a waste heat recovery system was devised and the performances of components incorporated to recover the heat generated during the processing of aerobic liquid-composting in a livestock manure treatment facility were analyzed. In addition, the availability of recovered heat was confirmed. The heat generated by liquid fermentation in the livestock manure treatment facility was also checked. Experimental temperatures were set at 35, 40, and 45 ℃ based on considerations of the uniformity of aerobic liquid-composting fermentation tank temperature and its operating range (34.5 ~ 43.9 ℃). Recovered heat energies from the combined heat exchanger, which consisted of PE and STS pipes, were 53.5, 65.6, 74.4 MJ/h, The heat pump of capacity 5 RT was heated at 95.6, 96.1, 98.9 MJ/h and the heating COPs of the pump were 4.53, 4.62, and 4.65, respectively. The maximum hot water production capacity of the heat exchanger assuming a fermentation tank temperature of 45 ℃ confirmed an energy supply of 56 360 kcal/day. The heating capacity of the FCU linked to the heat storage tank was 20.8 MJ/h, and the energy utilization efficiency was 96.1%. When livestock manure was dried using the FCU, it was confirmed that the initial function rate was reduced by 50.5 to 45.8 % after drying.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.8
• /
• pp.449-455
• /
• 2019

The increase in impermeable pavement from recent urbanization has resulted in an increase in surface runoff. The surface runoff has also increased the burden of the existing drainage system. This drainage system has structural limitations in that the catchment area is reduced by the waste particles transported with the surface runoff. In addition, the efficiency of the drainage system is decreased. To overcome these limitations, a new type of drainage system with a drainage layer was developed and applied. In this study, various volume porosity and permeability of the lower drainage layer were simulated using ANSYS CFX, which is a three dimensional computational fluid dynamics program. The results showed that the outlet velocity of the 35% volume porosity was faster than that of the 20% and 50% cases, and there was no relationship between the volume porosity and drainage performance. The permeability of the drainage layer can be determined from the particle size of the material, and a simulation of five conditions showed that 2 mm sand grains are most suitable for workability and usability. This study suggests appropriate values of the volume porosity and particle size of the drainage layer. This consideration can be advantageous for reducing and preventing flood damage.

• Korean journal of applied entomology
• /
• v.58 no.1
• /
• pp.69-76
• /
• 2019

This study was evaluated to increase the production efficiency of Gryllus bimaculatus De Geer, which are recently increasing attention as industrial insects in Korea. In this study, the hatching rate and larval period were investigated along with temperature and humidity, as well as the survival rate and ovipositional temperatures along with rearing density. The optimum relative humidity for hatching was highest at 90%, which shows the hatching rate of 90%. The highest hatching rate was 98.3% at $20^{\circ}C$. The hatching period was shortest at $35^{\circ}C$ incubation, which shows 7.1 days in average. The survival rate at 3,000-20,000 individuals in the breeding container was 34-18% after rearing for 35 days. In the effect test of feeding vegetables, the survival rate was 1.8 times and the biomass weight 2.5 times higher than that of normal individuals respectively. The number of laying eggs by temperature was highest at $25^{\circ}C$, which shows 1,710 eggs after reading for 30 days.

• Journal of Korea Technology Innovation Society
• /
• v.22 no.2
• /
• pp.159-185
• /
• 2019

Chemical industry, as one of the basis industries of the country, is characterized by converged high-end technology and large scale capital investment. In 2018, the government announced "The Strategy for Chemical Industry Development" with the vision of 'An Upgrade to the Advanced Chemical Industry'. In the meantime, the advanced chemical industry in Korea has been developed not by its own R&D, but by the upgrading of technologies and processes through the technology transfer from developed countries. The method of technology transfer was mainly achieved with international joint ventures (hereafter IJV) linked with technology transferors from developed countries. As it is necessary to grasp the determinants of the technology transfer and their importance to promote the transfers through IJVs, this relative importance analysis study was conducted by using the method of quantifying experts' opinions (AHP) on the determinants of the technology transfer of both parties. As a result of the analysis, in the Hierarchy1(PEST factors), the technology transferees focus on economic factors such as 'profitability of technology transfer' and 'cost efficiency', while technology transferors considered technical factors such as 'operational excellence (abilities to continuously improve technology process)'. In addition, transferors and transferees have different priorities for technology transfer determinants in all areas of Hierarchy 2 (sub-determinants). This suggests that Korean government or enterprise should shift the policy direction toward 'technology-oriented' when they promote advanced-technology transfer through IJVs.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.12 no.5
• /
• pp.482-492
• /
• 2019

The naval ship consists of a variety of classes, including patrol, frigate, destroyer and landing ship. There are various means of communication between combat ships belonging to the mobile fleet, including voice network, text message network and tactical data link. The operation at the existing mobile fleet level was conducted mainly on voice and text message, and tactical data link were used mainly to share tactical information between combat ships. This study intended to design HCI for commander like as KDDX of fleet level based on tactical data link, a means of communication between ships, to secure operational control of the mobile fleet. Recently, naval ships have been developed in the organization of various sensors, weapons and tactical data links integrated into the combat management system, and this study also designed the HCI based on the tactical data links integrated in the combat management system. The purpose of this study was to secure the operational efficiency of the tactical data link based on the combat management system in operation of the mobile fleet, and to improve the capability of the fleet commander to recognize the battlefield situation awareness.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.32 no.3
• /
• pp.191-197
• /
• 2019

As the shipbuilding and offshore plant industries grow larger and more complex, their maintenance and inspection systems become more important. Recently, maintenance and inspection systems based on augmented reality have been attracting much attention for improving worker's understanding of work and efficiency, but it is often difficult to work with because accurate matching between the augmented model and reality information is not. To solve this problem, marker based AR technology is used to attach a specific image to the model. However, the markers get damaged due to the characteristic of the shipbuilding and offshore plant industry, and the camera needs to be able to detect the entire marker clearly, and thus requires sufficient space to exist between the operator. In order to overcome the limitations of the existing AR system, in this study, a markerless AR was adopted to accurately recognize the actual model of the pipe system that occupies the most processes in the shipbuilding and offshore plant industries. The matching methodology. Through this system, it is expected that the twist phenomenon of the augmented model according to the attitude of the real worker and the limited environment can be improved.

• Journal of Wetlands Research
• /
• v.21 no.2
• /
• pp.181-190
• /
• 2019

Recently, Low impact development techniques, a form of nature-based solutions (NBS), were seen cost-efficient alternatives that can be utilized as alternatives for conventional stormwater management practices. This study evaluated the effectiveness of an infiltration trench (IT) and a small constructed wetland (SCW) in treating urban stormwater runoff. Long-term monitoring data were observed to assess the seasonal performance and cite the advantages and disadvantages of utilizing the facilities. Analyses revealed that the IT has reduced performance during the summer season due to higher runoff volumes that exceeded the facility's storage volume capacity and caused the facility to overflow. On the other hand, the pollutant removal efficiency of the SCW was impacted by the winter season as a result of dormant biological activities. Sediment data also indicated that fine and medium sand particles mostly constituted the trapped sediments in the pretreatment and media zones. Sediments in SCW exhibited a lower COD and TN load due to the phytoremediation and microbiological degradation capabilities of the system. This study presented brief comparison LID facilities equipped with pre-treatment zones. The identified factors that can potentially affect the performance of the systems were also beneficial in establishing metrics on the utilization of similar types of nature-based stormwater management practices.

• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.4
• /
• pp.494-500
• /
• 2019

Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genomewide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.3
• /
• pp.373-381
• /
• 2019

Site-directed mutagenesis was employed to generate five different triple point mutations in the double mutant (C295A/I86A) of Thermoanaerobacter ethanolicus alcohol dehydrogenase (TeSADH) by computer-aided modeling with the aim of widening the small alkyl-binding pocket. TeSADH engineering enables the enzyme to accept sterically hindered substrates that could not be accepted by the wild-type enzyme. The underline in the mutations highlights the additional point mutation on the double mutant TeSADH introduced in this work. The catalytic efficiency ($k_{cat}/K_M$) of the ${\underline{M151A}}$/C295A/I86A triple TeSADH mutant for acetophenone increased about 4.8-fold higher than that of the double mutant. A 2.4-fold increase in conversion of 3'-methylacetophenone to (R)-1-(3-methylphenyl)-ethanol with a yield of 87% was obtained by using ${\underline{V115A}}$/C295A/I86A mutant in asymmetric reduction. The ${\underline{A85G}}$/C295A/I86A mutant also produced (R)-1-(3-methylphenyl)-ethanol (1.7-fold) from 3'-methylacetophenone and (R)-1-(3-methoxyphenyl)-ethanol (1.2-fold) from 3'-methoxyacetophenone, with improved yield. In terms of thermal stability, the ${\underline{M151A}}$/C295A/I86A and ${\underline{V115A}}$/C295A/I86A mutants significantly increased ${\Delta}T_{1/2}$ by $+6.8^{\circ}C$ and $+2.4^{\circ}C$, respectively, with thermal deactivation constant ($k_d$) close to the wild-type enzyme. The ${\underline{M151A}}$/C295A/I86A mutant reacts optimally at $70^{\circ}C$ with almost 4 times more residual activity than the wild type. Considering broad substrate tolerance and thermal stability together, it would be promising to produce (R)-1-(3-methylphenyl)-ethanol from 3'-methylacetophenone by ${\underline{V115A}}$/C295A/I86A, and (R)-1-phenylethanol from acetophenone by ${\underline{M151A}}$/C295A/I86A mutant, in large-scale bioreduction processes.