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Evaluation for the Usefulness of Automated Blood Typing Analyzer (혈액은행 자동화 장비 도입의 유용성 평가)

  • Kim, Ha-na;Kim, Hee-Bum;Park, Hyun-Sang;Lee, Hyun-Im;Hong, Myung-Kook;Shin, Gyoung-Sook;Suh, In-Bum
    • The Journal of the Korea Contents Association
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    • v.19 no.6
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    • pp.565-574
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    • 2019
  • In this study, we evaluated the usefulness of an automatic blood typing analyzer using QWALYS-2 up (Diagast, Loos Cedex, France). During a month( 01OCT2013 - 31OCT2013) we performed 1,636 tests for ABO & RhD blood typing, 1,374 tests for antibody screen & identification tests and compared the results by automatic blood type analyzer with previous manual methods and column agglutination tests. And we analyzed the economic performance by comparison the test unit price between automatic blood type analyzer and manual methods. In ABO & RhD blood typing tests, there were complete concordances between manual and automated blood typing analyzer for 200 clinical samples. In Antibody screen tests, the concordance rate between manual and automated blood typing analyzer was 98.5% and more strong reaction in automated blood typing analyzer than manual methods. Therefore, the introduction of an automated blood typing analyzer, reagents costs were increased but labor costs were decreased. Considering the importance of transfusion safety and economic advantages, the introduction of an automated blood typing analyzer was very useful.

Vi-Phage Type Distribution and Antibiotic Susceptibility of Salmonella typhi in Korean(1982) (장티푸스균이 Vi-phage형 및 항생제감수성에 관한 연구)

  • Lee, B.K.;Kim, B.H.;Chung, T.H.
    • The Journal of the Korean Society for Microbiology
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    • v.18 no.1
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    • pp.39-46
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    • 1983
  • We collected three hundred thirty-five strains of Salmonella typhi isolated from human sources during the period January to December 1982 Korea. Most of them were from general hospitals and city health center, the remaining one hundred sixtyone strains were obtained from other 12 provincial health centers among 335 testing strains. We used ninety-nine Vi-phages as distributed from International Center for Enteric phage Typing(ICEPT) in London. We found nine phage types among 335 strains of Salmonella typhi in this study. Additionally, I+IV, degraded Vi-positive and Vi negative strains were presented. This study documented the occurrence of the new $B_2$ type in Korea for the first time. The present basic phage type formula in Korea appeared to be A, $B_2,\;D_1,\;D_2,\;D_4,\;D_6,\;D_8,\;D_{12},\;E_1,\;E_9,\;D_1,\;M_1$, 40 and plus I+IV and degraded vi strains(Table 2). The current phage types of Salmonella typhi isolated in Korea 1982 were A, $B_2,\;D_1,\;D_2,\;D_6,\;D_8,\;E_1,\;M_1$ and 46 $M_1$ type was widely distributed all over the country, and E type was next predominant. Antibiotic susceptibility test were performed by means of Kirby-Bauer disc diffution method using 12 kind of antibiotics such as Ampicillin, Carbenicillin, Cephalosporin, Chloramphenicol, Colistin, Gentamicin, Kanamycin, Nalidix acid, Neomycin, Polymyxin-B, Streptomycin and Tetracycline. The sensitivity pattern to antibiotics of Salmonelia typhi cultures were summarized.

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The Utility of Perturbation, Non-linear dynamic, and Cepstrum measures of dysphonia according to Signal Typing (음성 신호 분류에 따른 장애 음성의 변동률 분석, 비선형 동적 분석, 캡스트럼 분석의 유용성)

  • Choi, Seong Hee;Choi, Chul-Hee
    • Phonetics and Speech Sciences
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    • v.6 no.3
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    • pp.63-72
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    • 2014
  • The current study assessed the utility of acoustic analyses the most commonly used in routine clinical voice assessment including perturbation, nonlinear dynamic analysis, and Spectral/Cepstrum analysis based on signal typing of dysphonic voices and investigated their applicability of clinical acoustic analysis methods. A total of 70 dysphonic voice samples were classified with signal typing using narrowband spectrogram. Traditional parameters of %jitter, %shimmer, and signal-to-noise ratio were calculated for the signals using TF32 and correlation dimension(D2) of nonlinear dynamic parameter and spectral/cepstral measures including mean CPP, CPP_sd, CPPf0, CPPf0_sd, L/H ratio, and L/H ratio_sd were also calculated with ADSV(Analysis of Dysphonia in Speech and VoiceTM). Auditory perceptual analysis was performed by two blinded speech-language pathologists with GRBAS. The results showed that nearly periodic Type 1 signals were all functional dysphonia and Type 4 signals were comprised of neurogenic and organic voice disorders. Only Type 1 voice signals were reliable for perturbation analysis in this study. Significant signal typing-related differences were found in all acoustic and auditory-perceptual measures. SNR, CPP, L/H ratio values for Type 4 were significantly lower than those of other voice signals and significant higher %jitter, %shimmer were observed in Type 4 voice signals(p<.001). Additionally, with increase of signal type, D2 values significantly increased and more complex and nonlinear patterns were represented. Nevertheless, voice signals with highly noise component associated with breathiness were not able to obtain D2. In particular, CPP, was highly sensitive with voice quality 'G', 'R', 'B' than any other acoustic measures. Thus, Spectral and cepstral analyses may be applied for more severe dysphonic voices such as Type 4 signals and CPP can be more accurate and predictive acoustic marker in measuring voice quality and severity in dysphonia.

Evaluation of IH-1000 for Automated ABO-Rh Typing and Irregular Antibody Screening (ABO 및 RhD 혈액형 검사와 비예기항체 선별검사를 위한 자동화장비 IH-1000의 평가)

  • Park, Youngchun;Lim, Jinsook;Ko, Younghuyn;Kwon, Kyechul;Koo, Sunhoe;Kim, Jimyung
    • The Korean Journal of Blood Transfusion
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    • v.23 no.2
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    • pp.127-135
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    • 2012
  • Background: Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening. Methods: In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening. Results: For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively. Conclusion: The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.

Personal identification of the excavated ancient human bone through molecular-biological methods (분자생물학적 방법을 통한 출토인골의 개인 동정-사천 늑도 출토 인골과 민통선 민묘 출토 인골을 중심으로)

  • Seo, Min-Seok;Lee, Kyu-Shik;Chung, Yong-Jae;Lee, Myeong-Hui
    • 보존과학연구
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    • s.22
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    • pp.27-40
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    • 2001
  • DNA typing is often used to determine identity from human remains. Recently, the molecular biological analysis of ancient deposits has become possible since methods for the recovery of DNA conserved in bones or teeth from archaeological remains have been developed. In the field of archaeology, one of the most promising approaches is to identify the individuals present in a mass burial site. We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from a Korea ancient human remain excavated from Sa-chon Nuk-island and civilian access controlline(CACL). A femur bone were collected and successfully subjected to DNA extraction, quantification, PCR amplification, and subsequently typed for several shot tandem repeat(STR)loci. 4 types of STR systems used in this study were CTT multiplex(CSF1PO, TPOX, TH01), FFv multiplex(F13A01, FESFPS, vWA), Silver STRⅢ multiplex(D16S539, D7S820, D13S317), and amelogenin for sex determination. This studies are primarily concerned with the extraction, amplification, and DNA typing of ancient human bone DNA samples. Also, it is suggestive of importance about closely relationship between both fields of archaeology and molecular biology.

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Development and Evaluation of the Reader for Blood Typing using Column Agglutination Method (원주응집법을 이용한 혈액형 검사 판독기의 개발 및 평가)

  • Kim, Jin;Jeon, Wooram;Park, Seoung Woo;Lee, Chang Youl;Lee, Da-Hyeon;Choi, In-Taek;Kim, Ju Yeon;Suh, In Bum
    • The Journal of the Korea Contents Association
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    • v.14 no.3
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    • pp.346-351
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    • 2014
  • Accurate blood typing tests are essential for safe blood transfusion. Recently many automated test equipments have been introduced to reduce errors and increase the efficiency of the test. However, those equipments being high in price, it is difficult to introduce automated test equipment for every hospital. In this study, we developed a reader for blood typing using column agglutination test. In the process, the results, read out by the image processing, are stored and reaffirmed. To evaluate the reader, 148 samples for ABO and RhD blood typing tests and 154 samples for unexpected antibody test were used. The positive and negative intensity of the reading and the reading of the reaction were 100% in agreement with the result of traditional manual method. If additional verification is completed, this reader can be efficiently and economically used in small-and medium-sized hospitals.

High Resolution Whole Genome Multilocus Sequence Typing (wgMLST) Schemes for Salmonella enterica Weltevreden Epidemiologic Investigations

  • Tadee, Pakpoom;Tadee, Phacharaporn;Hitchings, Matthew D.;Pascoe, Ben;Sheppard, Samuel K.;Patchanee, Prapas
    • Microbiology and Biotechnology Letters
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    • v.46 no.2
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    • pp.162-170
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    • 2018
  • Non-typhoidal Salmonella is one of the main pathogens causing food-borne illness in humans, with up to 20% of cases resulting from consumption of pork products. Over the gastroenteritis signs, multidrug resistant Salmonella has arisen. In this study, pan-susceptible phenotypic strains of Salmonella enterica serotype Weltevreden recovered from pig production chain in Chiang Mai, Thailand during 2012-2014 were chosen for analysis. The aim of this study was to use whole genome sequencing (WGS) data with an emphasis on antimicrobial resistance gene investigation to assess their pathogenic potential and genetic diversity determination based on whole genome Multilocus Sequence Typing (wgMLST) to expand epidemiological knowledge and to provide additional guidance for disease control. Analyis using ResFinder 3.0 for WGS database tracing found that one of pan-susceptible phenotypic strain carried five classes of resistance genes: aminoglycoside, beta-lactam, phenicol, sulfonamide, and tetracycline associated genes. Twenty four and 36 loci differences were detected by core genome Multilocus Sequence Typing (cgMLST) and pan genome Multilocus Sequence Typing (pgMLST), respectively, in two matching strains (44/13 vs A543057 and A543056 vs 204/13) initially assigned by conventional MLST and Pulsed-field Gel Electrophoresis (PFGE). One hundread percent discriminant ability can be achieved using the wgMLST technique. WGS is currently the ultimate molecular technique for various in-depth studies. As the findings stated above, a new of "gold standard typing method era" for routine works in genome study is being set.

Evaluation of the Automated Immunohematology Analyzer DAYMATE M (혈액은행 자동화 검사장비 DAYMATE M의 수행능 평가)

  • Yoo, Jaeeun;Yu, Hain;Choi, Hyunyu;Lee, Gyoo Whung;Song, Young-Sun;Lee, Seungok;Jekarl, Dong Wook;Kim, Yonggoo
    • Laboratory Medicine Online
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    • v.7 no.4
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    • pp.163-169
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    • 2017
  • Background: An automated immunohematology analyzer, DAYMATE M (DAY Medical, Switzerland), has been recently developed. The potential of this analyzer to improve test results has been evaluated. Methods: A total of 300 blood samples from Seoul St. Mary's hospital and Incheon St. Mary's hospital were tested for ABO and RhD typing. In addition, 336 antibody screening test (AST) samples and 82 patients treated with hematopoietic stem cell transplantation (HSCT) were included. AST results by DAYMATE M were compared with those obtained by a manual method using DS-Screening II (Bio-Rad Laboratories, Switzerland) and red blood cells from Selectogen (Ortho-Clinical diagnostics Inc., USA). Results: Of the 300 patients enrolled, 87, 73, 79, and 61 had type A, B, O, and AB blood, respectively. The concordance rate was 99.9% for cell typing and 97.0% for serum typing. One discordant case was classified as type B instead of AB, and six discordant serum-typing cases were type A, but classified as type AB. Among the 336 AST samples, the concordance rate was 93.2%. From 136 positive cases, six were discordant. Within the 82 HSCT-treated patients, the concordance rate for ABO blood typing was 92.2%. Among the six discordant cases, DAYMATE M typed four cases as donor type where the standard method typed them as the recipient blood type. Conclusions: The DAYMATE M automated immunohematology analyzer performs reliably for ABO and RhD typing, as well as for ASTs and on samples from patients treated with HSCT.

Molecular typing and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from bovine mastitis (유방염 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출)

  • Kim, Sin;Hong, Hyon-Pyo;Kim, Sang-Yun;Kwon, Heon-Il;Lee, Hee-Moo
    • Korean Journal of Veterinary Service
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    • v.25 no.3
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    • pp.275-283
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    • 2002
  • Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.