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Molecular typing and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from bovine mastitis  

Kim, Sin (Northern Branch, Gyeongbuk Veterinary Service Laboratory)
Hong, Hyon-Pyo (Dept of Biology, Andong National University)
Kim, Sang-Yun (Dept of Biology, Andong National University)
Kwon, Heon-Il (Dept of Biology, Andong National University)
Lee, Hee-Moo (Dept of Biology, Andong National University)
Publication Information
Korean Journal of Veterinary Service / v.25, no.3, 2002 , pp. 275-283 More about this Journal
Abstract
Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.
Keywords
Staphylococcus aureus; Enterotoxin; Multiplex PCR; PFGE;
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