• Restorative Dentistry and Endodontics
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• 제33권2호
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• pp.125-132
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• 2008

이 연구의 목적은 서로 다른 접착제들로 수리된 복합 레진의 전단 결합 강도를 비교하고, 접착 시스템이 복합레진의 수리 강도에 미치는 영향을 평가하기 위한 것이다. 40개의 복합 레진 시편 (Z-250)을 준비하였으며, 1주일 동안 열순환을 시행한 후, $50\;{\mu}m$ 알루미나 입자로 샌드 블라스팅 하였다. 이 시편들을 네 개의 그룹으로 나누었으며 (n = 10), 다음과 같은 서로 다른 종류의 접착제를 각각 적용하였다; SB 그룹 : Scotchbond multipurpose ; SE 그룹 : Clearfil SE bond ; XP 그룹 : XP bond ; XE 그룹 : Xeno III. 접착 과정을 완료한 후에, 새로운 레진 (Z-250, 3M ESPE)을 몰드에 적용하여, 광조사 하였다. 대조군으로, 접착 과정 없이 10개의 시편을 준비하였다. 7일 동안 열순환을 시행한 후 전단 결합 강도를 측정하였고, 파절 양상을 조사하였으며, 다음과 같은 결과를 얻었다. 1. 기존 복합 레진과 수리용 복합 레진 사이의 전단 결합 강도는 접착제 종류에 따른 유의한 차이를 보이지 않았다. 2. 재료의 응집 강도 (대조군)와 수리한 결합 강도 (실험군) 사이에 유의한 차이를 보이지 않았다. 3. 파절 양상은 대부분 응집성 파절 또는 혼합 파절을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4331-4338
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• 2014

$N^1$-(2, 5-dimethoxyphenyl)-$N^8$-hydroxyoctanediamide (N25) is a novel SAHA cap derivative of HDACi, with a patent (No. CN 103159646). This invention is a hydroxamic acid compound with a structural formula of $RNHCO(CH_2)6CONHOH$ (wherein R=2, 5dimethoxyaniline), a pharmaceutically acceptable salt which is soluble. In the present study, we investigated the effects of N25 with regard to drug distribution and molecular docking, and anti-proliferation, apoptosis, cell cycling, and $LD_{50}$. First, we designed a molecular approach for modeling selected SAHA derivatives based on available structural information regarding human HDAC8 in complex with SAHA (PDB code 1T69). N25 was found to be stabilized by direct interaction with the HDAC8. Anti-proliferative activity was observed in human glioma U251, U87, T98G cells and human lung cancer H460, A549, H1299 cells at moderate concentrations ($0.5-30{\mu}M$). Compared with SAHA, N25 displayed an increased antitumor activity in U251 and H460 cells. We further analyzed cell death mechanisms activated by N25 in U251 and H460 cells. N25 significantly increased acetylation of Histone 3 and inhibited HDAC4. On RT-PCR analysis, N25 increased the mRNA levels of p21, however, decreased the levels of p53. These resulted in promotion of apoptosis, inducing G0/G1 arrest in U251 cells and G2/M arrest in H460 cells in a time-dependent and dose-dependent manner. In addition, N25 was able to distribute to brain tissue through the blood-brain barrier of mice ($LD_{50}$: 240.840mg/kg). In conclusion, our findings demonstrate that N25 will provide an invaluable tool to investigate the molecular mechanism with potential chemotherapeutic value in several malignancies, especially human glioma.

• Korean Chemical Engineering Research
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• 제55권3호
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• pp.307-312
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• 2017

본 연구에서는 PFO (pyrolyzed fuel oil)를 이용해 탄소 전구체(피치)를 얻은 후 KOH와 $K_2CO_3$를 이용한 화학적 활성화를 통해 표면 개질한 카본의 전기화학적 특성을 분석하였다. 탄소 전구체는 3903, 4001, 4002의 세 종류를 사용하였으며, 각 각 PFO를 $390^{\circ}C$ 3 시간, $400^{\circ}C$ 1시간, $400^{\circ}C$ 2 시간 열처리 하여 제조하였다. 또한 화학적 활성화 실험은 활성 촉매의 종류, 교반시간 등을 변화시키면서 비표면적 및 기공크기 등의 물성이 전기화학적 특성에 미치는 효과를 조사 하였다. 제조된 표면개질 PFO 피치의 물리적 특성은 BET, FE-SEM 등을 통해 분석되었으며, 음극 소재로서의 전기 화학적 성능은 충 방전, 순환전압전류, 임피던스, 속도 테스트를 통해 조사되었다. 화학적 활성화법을 이용해 제조한 카본의 평균 기공크기는 22 nm, 비표면적은 $3.12m^2/g$의 결과를 얻었다. 세 가지 개질된 석유계 피치를 음극소재로 사용하여 조사된 전기화학적 특성은 4001 피치가 가장 우수한 것으로 나타났으며, 이 때 표면개질 조건은 KOH를 사용하여 2시간 교반 후 화학적 활성화법에 의하여 열처리 하였다. KOH를 이용한 표면개질 PFO 피치를 사용해 제조한 전지의 초기 용량은 318 mAh/g, 초기효율은 80%로 우수한 결과를 보였으며, 2C/0.1C 속도 테스트 특성은 92%로 높은 특성을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1711-1714
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• 2014

Objective: To explore the effect of Withaferin A on A549 cellular proliferation and apoptosis in non-small cell lung cancer (NSCLC). Materials and Methods: NSCLC cell line A549 was selected to explore the effect of Withaferin A on A549 cellular proliferation, apoptosis and the PI3K/Akt signal pathway capable of regulating tumor biological behavior by assessment of cellular proliferation, cellular apoptotic rates and cellular cycling as well as by immuno-blotting. Results: Withaferin A could inhibit A549 cellular proliferation and the control rate was dosage-dependent (P<0.05), which also increased time-dependently with the same dosage of Withaferin A (P<0.05). The apoptotic indexes in A549 cells treated with 0, 2.5, 5.0, 10.0 and 20.0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A for 48 h were significantly different (P<0.05). In addition, the apoptotic rates of each group in both early and advanced stages were higher than those in 0 ${\mu}mol{\cdot}L^{-1}$ (P<0.05), which were evidently higher after 48 h than those after 24 h (P<0.05). A549 cells treated by Withaferin A for 48 h were markedly lower in Bcl-2 level and obviously higher in Bax and cleaved caspase-3 levels than those treated by 0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05), and there were significant differences among 5, 10 and 20 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05). The ratios of A549 cells treated by Withaferin A for 48 h in G0/G1 stage were higher than those in 0 ${\mu}mol{\cdot}L^{-1}$, while those in S and G2/M stages were obviously lower than those in G2/M stage, and there were significant differences in 5.0, 10.0 and 20.0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05). Additionally, p-Akt/Akt values were in reverse association with dosage, and the differences were significant (P<0.05). Conclusion: Withaferin A can inhibit the proliferation and apoptosis of A549 cells by suppressing activation of the PI3K/Akt pathways.

• 한국환경보건학회지
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• 제43권2호
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• pp.143-156
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• 2017

The purpose of this study was to investigate water quality through a field survey at six floor fountain sites. The floor fountain facilities were designed and operated in such a way that tap water was stored in a water tank and recycled repeatedly. The water tank was cleaned once or twice per week in the summer. The number of facility users was low during the day due to sweltering heat, but up to 40 people, mainly children, were using them around 8 pm. Since the operation time was as short as 30 minutes, it is considered necessary to extend it for at least one hour for the number of users. As a result of the water quality test of the reservoir tank prior to operation after cleaning, it was measured to be within drinking water quality standards at the six facilities. As a result of the water quality test after use, ammonia nitrogen was measured to be 1.45 mg/L at Site IV. This exceeded the drinking water quality standard of 0.5 mg/L. In the case of turbidity, two cases exceed at 7.38 and 4.52 NTU when applying 4 NTU as the water quality standard for waterscape facilities. Twenty-eight cases exceed the standard of drinking water quality. The result of microbiological tests, at five sites excepting Site I, where disinfectant was injected, was that the maximum total colony count was 180,000 CFU/mL, total coliforms was 2,100,000 CFU/100 mL, fecal coliforms was 4,600 CFU/100 mL, Escherichia coli was 170 MPN/100 mL and Enterococcus was 100 CFU/100 mL. This exceeded the water quality standards of drinking water. Children are very likely to inhale because the water spews from below and falls from above, so it is necessary to apply water quality standards for ammonia nitrogen, turbidity and microbes. Current floor fountain facilities are highly susceptible to disease caused by microbial contamination because of water cycling and reuse, so it is necessary to change the water every day, clean the water tank, and perform chlorination. Therefore, it is necessary to inject calcium hypochlorite according to the free chlorine water quality standard of swimming pools with a different water tank capacity. In addition, facilities should be improved to prevent the reuse of water by installing the water tank at a separate location.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6007-6012
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• 2013

ANXA2, a member of the annexin family, is overexpressed and plays important roles in tumor development. However, the significance of ANXA2 expression in gastric carcinoma has not been clarified.To elucidate its roles in growth of gastric cancer, ANXA2 expression in SGC-7901 cells was inhibited with a designated siRNA, then cell proliferation, cell cycling, apoptosis and motility were determined by MTT assay, flow cytometry, Hoechst 33342 staining and wound healing assay, respectively. To further assess the behavior of ANXA2 deleted SGC-7901 cells, changes of microstructures were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. We found that inhibition of ANXA2 expression caused cell proliferation to decrease significantly with G1 arrest, motility to be reduced with changes in pseudopodia/filopodia structure and F-actin and ${\beta}$-tubulin expression, and apoptosis to be enhanced albeit without significance. At the same time, ANXA2 deletion resulted in fewer pseudopodia/filopodia, non-stained areas were increased, contact inhibition among cells reappeared, and expression of F-actin and ${\beta}$-tubulin was decreased, with induction of polymerized disassembled forms. Taken together, these data suggest that ANXA2 overexpression is important to maintain the malignancy of cancer cells, and this member of the annexin family has potential to be considered as a target for the gene therapy of gastric carcinoma.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5915-5920
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• 2013

Aim: Although aberrant miRNA expression has been documented, altered miR-101 expression in cervical cancer and its carcinogenic effects and mechanisms remain unexplored. The aim of our study was to investigate the role of miR-101 alteration in cervical carcinogenesis. Methods: Expression of miR-101 was examined by quantitative real-time reverse transcriptase PCR (qRT-PCR) in Hela cells. After modulating miR-101 expression using miR-101 mimics, cell growth, apoptosis and proliferation, and migration were tested separately by MTT or flow cytometry and cell wound healing assay and protein expression was detected by qRT-PCR. The expression of COX-2 in Hela cell was also examined by immunohistochemical staining and the correlation with miR-101 expression was analysed. Results: The miR-101 demonstrated significantly low expression in Hela cell. When we transfected miR-101 mimics into Hela cells, the modulation of miR-101 expression remarkably influenced cell proliferation, cycling and apoptosis: 1) The expression of microRNA-101 tended to increase after transfection; 2) Overexpression of miR-101 was able to promote cell apoptosis, the apoptosis rate being markedly higher (97.6%) than that seen pre-transfection (12.2%) (P<0.05); 3) The miR-101 negatively regulates cell migration and invasion, scratch results being lower ($42.7um{\pm}2um$) than that observed pre-transfection ($181.4um{\pm}2um$); 4) miRNA-101 inhibits the proliferation of Hela cells as well as the level of COX-2 protein, which was negatively correlated with miR-101 expression. Conclusions: Overexpression of miR-101 has obvious inhibitory effects on cell proliferation, migration and invasion. Thus reduced miR-101 expression could participate in the development of cervical cancer at least partly through loss of inhibition of target gene COX-2, which probably occurs in a relative late phase of carcinogenesis. Our data suggest an important role of miR-101 in the molecular etiology of cancer and indicate potential application of miR-101 in cancer therapy.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1205-1209
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• 2000

The present study was undertaken to investigate the effect of stimulation of follicular development with eCG on the peripheral levels of inhibin and FSH in Murrah buffaloes. Estrus was synchronized in five normally cycling females by insertion of Crestar (Intervet, Boxmeer, Holland) implants for nine days. Estradiol valerate was administered i.m. on the day of implant insertion. On the 10th day of the induced estrous cycle a single dose of 3000 IU eCG (Folligon, Intervet, Boxmeer, Holland) was given, followed by treatment with 25 mg of $PGF_2$ alpha (Lutalyse, Upjohn, Belgium) 48 h later. Blood samples were obtained during the induced estrus, on cycle day 10 (luteal phase), at the superovulatory estrus (43 h after PGF) and during the periovulatory period (64 h after PGF). Ultrasonography was done daily to monitor follicular development. Plasma concentrations of inhibin and FSH were determined by specific radioimmunoassays. Differences between $mean{\pm}SEM$ values of different phases of the cycle were compared by ANOVA. The mean number of small (2-5 mm), medium (6-9 mm) and large (>10 mm) follicles observed two days after eCG treatment and on the day of superovulatory estrus was $2.8{\pm}0.31$, $5.2{\pm}0.30$ and $1.4{\pm}0.09$ and $1.9{\pm}0.21$, $2.8{\pm}0.40$ and $5.0{\pm}0.83$, respectively. The mean number of ovulations was $3.6{\pm}0.37$ and the mean number of unovulated follicles was $6.1{\pm}0.47$. Most of the follicles >10 mm in diameter had ovulated (72%). The mean ${\pm}SEM $ of plasma inhibin concentration $(2584.15{\pm}17.92pg/ml)$ during the superovulatory estrus was significantly higher $(p{\leq}0.05)$ than during the induced estrus $(749.87{\pm}17.29pg/ml)$, the luteal phase $(1099.54{\pm}24.98pg/ml)$ and periovulatory period $(1682.71{\pm}29.88pg/ml)$, respectively. $Mean{\pm}SEM$ plasma FSH concentration during the induced estrus $(10.35{\pm}0.41ng/ml)$ was not different from that during the superovulatory estrus $(8.52{\pm}0.39ng/ml)$, but was significantly higher $(p{\leq}0.05)$ than during the luteal phase $(2.81{\pm}0.42ng/ml)$ and periovulatory period $(5.7{\pm}0.28ng/ml)$. These data indicate that treatment with eCG in buffaloes for inducing superovulation results in a significant elevation in plasma inhibin levels and a decrease in plasma FSH levels during the superovulatory estrus. Thus, we suggest that the elevated plasma inhibin coming from fully developed follicles continued for a long time which results in inhibition of FSH leading to poor ovulation in the remaining follicles, which may be the cause of suboptimal superovulatory response.

• 한국유기농업학회지
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• 제25권2호
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• pp.373-386
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• 2017

본 시험은 전국의 광역친환경 농업단지 중에서 모범적으로 친환경 농업을 실천하고 있는 3곳(장흥, 순천, 옥천)의 벼 농가를 대상으로 시기별로 양분 분석을 하였다. 장흥지역 농가만이 경축자원 순환농업을 실천하고 있는 것으로 관찰되었다. 광역친환경 벼 농업단지의 3~9월 사이의 시기별 토양 pH는 5.5~6.7의 분포를 보였고, EC는 $0.4{\sim}1.0dS\;m^{-1}$으로 나타났다. 시기별 토양 전질소는 순천 농가에서 높았고 칼륨은 옥천 농가에서 낮은 수준을 보였다. 시기별 토양 암모늄태 질소는 봄철 비료 시용으로 6월 전후에 상승하였고, 장흥 농가에서 가장 높은 수준을 보이다가 이후 크게 감소하였다. 수량과 수확지수가 가장 높았던 옥천 농가에서 ha당 연간 조수입이 다른 농가보다 세배 가까이 높은 수준을 보였다. 비교적 많은 비료를 투입한 옥천 농가에서 시기별 양분수지가 높았고, 순천 농가의 ha당 칼륨 수지는 9월에 약 -60 kg까지 관찰되어 칼륨 결핍에 따른 생산성 저하에 일부 영향을 주었을 것으로 추정되었다. 시기별 전질소와 인산수지는 장흥 농가에서 가장 낮은 수준을 보였고 벼 낟알의 질소이용효율도 가장 높아서 광역친환경 농업단지의 추천할 만한 사례 농가로 평가되었다.

• 한국환경복원기술학회지
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• 제20권4호
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• pp.43-61
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• 2017

The wetlands are facing environmental changes such as desiccation that occurs with the passage of time and reduced ecosystem services from wetlands in the city. In order to maintain the ecosystem services provided by wetlands in urban areas, a system thinking about the trade-off phenomenon of ecosystem services occurring as the wetlands undergo environmental changes is needed. Therefore, the purpose of this study is to develop strategies for wetland design using system thinking approach to enhance the resilience of ecosystem services degraded by the desiccation of wetlands and other disturbances. The objectives of this study include the system boundary and variables. Second, analyzing the dynamics of wetland design strategy. Third, it analyzes the trade-off phenomenon of ecosystem services in terms of the hydrology, hydric soil, and plants strategies to mitigate these effects. Fourth, wetland basic design to improve the resilience of ecosystem services. A wetland in Abuk-Mountain Neighborhood Park, Miryang-si, Gyeongsangnam-do, has been selected as a case study. Causal loop diagrams(CLDs) are used to analyze feedback in the wetland regime. In summary, hydrology, hydric soil, and plants is suggested as system boundaries to design plan. Design strategies for the wetland focused on robustness, redundancy, rapidity, and resourcefulness as a result of CLD analysis are first proposed in order to effectively maintain the wetland regime over the long term. Secondly, in a section related to hydrology, the CLD results show the trade-offs between provisioning-cultural services and regulating services. In order to control these services, a "water cycling system" has been implemented due to its strength in terms of robustness. The CLDs for hydric soil showed the trade-offs between regulating services and supporting services. An "installation of storm drainage for maintaining water levels" was selected due to the strength offered in terms of redundancy and rapidity. The CLDs for plants showed the trade-offs between provisioning - cultural services and regulating services. In order to control the strategic points, the "planting of indigenous vegetation" was suggested given the strength in terms of redundancy. In this study, a wetland design method is proposed that can improve the resilience of wetland ecosystem services by analyzing the dynamics overtime. The results of this research can theoretically be applied to help restore ecosystem services in wetlands using ecological landscape design. In addition, this study will contribute to reducing maintenance costs by improving wetland resilience.