• 한국축산식품학회지
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• 제31권6호
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• pp.851-857
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• 2011

This study was conducted to develop a real time reverse transcriptase-PCR (RT-PCR) method for the detection of viable Enterobacteriaceae in milk using primers based on the genes of ribosomal proteins S11 and S13 and to determine effects of heating and subsequent treatments on the threshold cycle (Ct) of the real time RT-PCR. Total RNA was isolated from 17 strains of bacteria including 11 strains of Enterobacteriaceae suspended in milk using a modified Tri reagent method. SYBR Green Master Mix was added to the RNA and the mixture was subjected to the real time RT-PCR. The Cts of eleven type strains of the Enterobacteriaceae in milk ($10^7$ cells) in the real time RT-PCR ranged from 21.5 to 24.6. However, the Cts of Pseudomonas fluorescens, Acinetobacter calcoaceticus, and three gram-positive bacteria were more than 40. The real time RT-PCR detected as low as $10^3$ cells in agarose gel electrophoresis. The Cts increased from 22.0 to 34.2 when milk samples contaminated with Escherichia coli ($10^7$ cells/mL) were heated at $65^{\circ}C$ for 30 min. In addition, subsequent incubation at $37^{\circ}C$ for 6 and 24 h increased the Cts further up to 36.2 and 37.2, respectively. Addition of RNase A to the bacterial suspension obtained from the heated milk and subsequent incubation at $37^{\circ}C$ for 1 h increased the Cts to more than 40. The results of this study suggests that pretreatment of bacterial cells heated in milk with RNase A before RNA extraction might enhance the ability to differentiate between viable and dead bacteria using real time RT-PCR.

• 한국양식학회지
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• 제15권2호
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• pp.87-93
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• 2002

양어용수 재순환을 위한 생물여과상의 처리효율을 향상시키기 위하여 상향류식 연속역세여과장치를 이용하여 다양한 조건하에서 실험하였는데, 기존의 연속역세여과장치와 이를 개량한 연속역세여과장치를 가지고 실험하였다. 개량역세여과장치는 기존에 이용되고 있는 DynaSand 여과장치를 유체역학 및 물리,화학적 개념을 응용한 장치로 개조하여 역세기능의 향상, 산소전달 능력의 향상, 여과 처리수 인출부의 개량에 의한 처리수 탁도제거 기능 향상 및 연속적인 수두손실 감지에 의해 폐수성상에 따른 여과장치운전의 자동화를 도모하는데 중점을 두었고, 또한 여과사의 표면을 특정물질로 코팅함으로 여과사 표면전하를 변화시켜 여과능력의 향상과 생물막 형성력의 증대를 꾀하는데 실험의 목적을 두었다. 본 실험에서 사용 한 여과장치의 크기는 전체 용적이 약 70 L이고 working volume은 35 L이었다 이번 연구에서 양어장사육용수 처리를 위해 조사된 개량형 상향류식 연속역세여과장치의 효율은 부유물 제거 효율이 평균 71% 이었고, NH$_4$$^{+/-N}$ , T-P및 SRP의 제거효율은 84%, 85% 및 88%로 각각 나타나서 그 효과가 인정되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6247-6251
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• 2014

Background: The aim of the present study was to investigate the involvement of emodin on the growth of human breast cancer MCF-7 and MDA-MB-231 cells and the estrogen (E2) signal pathway in vitro. Materials and Methods: MTT assays were used to detect the effects of emodin on E2 induced proliferation of MCF-7 and MDA-MB-231 cells. Flow cytometry (FCM) was applied to determine the effect of emodin on E2-induced apoptosis of MCF-7 cells. Western blotting allowed detection of the effects of emodin on the expression of estrogen receptor ${\alpha}$, cyclin D1 and B-cell lymphoma-2 (Bcl-2), mitogen-activated protein kinases (MAPK) and phosphatidylinostiol 3-kinases (PI3K). Luciferase assays were emplyed to assess transcriptional activity of $ER{\alpha}$. Results: Emodin could inhibit E2-induced MCF-7 cell proliferation and anti-apoptosis effects, and arrest the cell cycle in G0/G1 phase, further blocking the effect of E2 on expression and transcriptional activity of $ER{\alpha}$. Moreover, Emodin influenced the ER ${\alpha}$ genomic pathway via downregulation of cyclin D1 and Bcl-2 protein expression, and influenced the non-genomic pathway via decreased PI3K/Akt protein expression. Conclusions: These findings indicate that emodin exerts inhibitory effects on MCF-7 cell proliferation via inhibiting both non-genomic and genomic pathways.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.5131-5136
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• 2012

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.

• Fisheries and Aquatic Sciences
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• 제20권8호
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• pp.16.1-16.8
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• 2017

The exportation of cultured olive flounder (Paralichthys olivaceus) in Korea has been recently decreasing due to the infections with a myxozoan parasite Kudoa septempunctata, and there is a strong demand for strict food safety management because the food poisoning associated with consumption of raw olive flounder harbouring K. septempunctata has been frequently reported in Japan. The life cycle and infection dynamics of K. septempunctata in aquatic environment are currently unknown, which hamper establishment of effective control methods. We investigated sea water and marine invertebrates collected from olive flounder farms for detecting K. septempunctata by DNA-based analysis, to elucidate infection dynamics of K. septempunctata in aquaculture farms. In addition, live marine polychaetes were collected and maintained in well plates to find any possible actinosporean state of K. septempunctata. The level of K. septempunctata DNA in rearing water fluctuated during the sampling period but the DNA was not detected in summer (June-July in farm A and August in farm B). K. septempunctata DNA was also detected in the polychaetes Naineris laevigata intestinal samples, showing decreased pattern of 40 to 0%. No actinosporean stage of K. septempunctata was observed in the polychaetes by microscopy. The absence of K. septempunctata DNA in rearing water of fish farm and the polychaetes N. laevigata intestinal samples during late spring and early summer indicate that the infection may not occur during this period. N. laevigata was suspected as the possible alternate invertebrate host of K. septempunctata, but the actinosporean stage was not found by well plate method and further studies will be necessary. This research provides important baseline information for understanding the infection dynamics of K. septempunctata in olive flounder farms and further establishment of control strategies.

• 대한의생명과학회지
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• 제9권1호
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• pp.29-36
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• 2003

Progesterone levels in the blood plasma or skim milk of cows are considered to be very useful indicator fur the detection of estrus cycle and early pregnancy diagnosis. During 13 to 14 days after estrus the level of progesterone in plasma or skim milk were not different between the inseminated arid non-inseminated cows. In the pregnant cows the peak level of progesterone reached on 14th day after artificial insemination (AI), but in the absence of conceptus the level declines after the 14th day slowly, and then very rapidly towards the basal level after the 17th day. This low level persists about 4 days, including those of estrus and ovulation a highly characteristic pattern which differs so markedly from that in the pregnant cows. Progesterone levels in blood plasma or skim milk can provided a reliable diagnosis of early pregnancy and monitoring ovarian activity in cows. The mean $\pm$ standard deviation of milk urea nitrogen(MUN) and protein concentration in the cows at 9 herds were 17.7$\pm$2.35 mg/dL and 3.2$\pm$0.17%, respectively. The days of nonpregnant after parturition was shorter in the cows in which the lower level of MUN than higher level of MUN concentration.

• Journal of Information Processing Systems
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• 제5권1호
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• pp.33-40
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• 2009

Ever since the network-based malicious code commonly known as a 'worm' surfaced in the early part of the 1980's, its prevalence has grown more and more. The RCS (Random Constant Spreading) worm has become a dominant, malicious virus in recent computer networking circles. The worm retards the availability of an overall network by exhausting resources such as CPU capacity, network peripherals and transfer bandwidth, causing damage to an uninfected system as well as an infected system. The generation and spreading cycle of these worms progress rapidly. The existing studies to counter malicious code have studied the Microscopic Model for detecting worm generation based on some specific pattern or sign of attack, thus preventing its spread by countering the worm directly on detection. However, due to zero-day threat actualization, rapid spreading of the RCS worm and reduction of survival time, securing a security model to ensure the survivability of the network became an urgent problem that the existing solution-oriented security measures did not address. This paper analyzes the recently studied efficient dynamic network. Essentially, this paper suggests a model that dynamically controls the RCS worm using the characteristics of Power-Law and depth distribution of the delivery node, which is commonly seen in preferential growth networks. Moreover, we suggest a model that dynamically controls the spread of the worm using information about the depth distribution of delivery. We also verified via simulation that the load for each node was minimized at an optimal depth to effectively restrain the spread of the worm.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.83-92
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• 2009

Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, respectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and $20/30\;{\mu}g/kg$). The cut-off values of the OS-ICG for the spiked corn were 5 and $10\;{\mu}g/kg$ for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.

• Parasites, Hosts and Diseases
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• 제47권4호
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• pp.323-335
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• 2009

A successful experience of lymphatic filariasis control in the Republic of Korea is briefly reviewed. Filariasis in the Republic of Korea was exclusively caused by infection with Brugia malayi. Over the past several decades from the 1950s to 2006, many investigators exerted their efforts to detection, treatment, and follow-up of filariasis patients in endemic areas, and to control filariasis. Mass, combined with selective, treatments with diethylcarbamazine to microfilaria positive persons had been made them free from microfilaremia and contributed to significant decrease of the microfilarial density in previously endemic areas. Significant decrease of microfilaria positive cases in an area influenced eventually to the endemicity of filariasis in the relevant locality. Together with remarkable economic growth followed by improvement of environmental and personal hygiene and living standards, the factors stated above have contributed to blocking the transmission cycle of B. malayi and led to disappearance of this mosquito-borne ancient disease in the Republic of Korea.

• 방사성폐기물학회지
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• 제2권3호
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• pp.219-230
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• 2004

Most of the low-level liquid radioactive wastes generated from PWR plants are classified into high or low total suspended solid(HTDS or LTDS), and into radiochemical and radioactive laundry waste. Although the evaporation process has a high decontami- nation ability, it has several problems such as corrosion, foam, and congestion. A new liquid waste disposal process using the ion-exchange demineralizer(IED), instead of the current evaporation process, has been introduced into the Yonggwang NPP #5 and 6. These two methods have been compared to understand the differences in this study. Aspects compared here were the released radioactivity amount of the liquid radioactive wastes, the dose of off-site residents, the decontamination factor, and the amount of the solid radioactive wastes. The IED system is designed to discharge higher radioactivity about 20% than the evaporating system, and the actual radioactivity released from the evaporating and IED system were 0.473mCi and 1.098mCi, respectively. The radioactivity released from the IED was 2.32 times higher than that of the evaporating system. The dose of off-site residents was $2.97{\times}10^{-6}$mSv for the evaporating system, and $6.47{\times}10^{-6}$mSv for IED. The decontamination factor(DF) of the evaporator is, in most cases, far lower than the lower limits of detection(LLD) with the Ge-Li detector. Due to the low concentration of the liquid wastes collected from the liquid waste system, the decontamination factor of IED is very low. Since there is not enough data on the amount of solid radioactive wastes generated by the evaporation system, the comparison on these two systems has been conducted on the basis of the design, and the comparison result was that the evaporating system generated more wastes about 40% than IED.