[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0802.105

One-Step Simultaneous Immunochromatographic Strip Test for Multianalysis of Ochratoxin A and Zearalenone

Shim, Won-Bo (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University)
Dzantiev, Boris B. (A.N.Bach Institute of Biochemistry, Russian Academy of Sciences)
Eremin, Sergei A. (Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University)
Chung, Duck-Hwa (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University)

Publication Information

Journal of Microbiology and Biotechnology / v.19, no.1, 2009 , pp. 83-92 More about this Journal

Abstract

Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, respectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and $20/30\;{\mu}g/kg$ ). The cut-off values of the OS-ICG for the spiked corn were 5 and $10\;{\mu}g/kg$ for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.

Keywords

One-step simultaneous immunoassay; ochratoxin A; zearalenone; immunochromatography strip; corn;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 9 (Related Records In Web of Science)

Reference
Cited By KSCI

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