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http://dx.doi.org/10.7314/APJCP.2012.13.10.5131

Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines  

Alabsi, Aied M. (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA))
Ali, Rola (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA))
Ali, Abdul Manaf (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA))
Al-Dubai, Sami Abdo Radman (Community Medicine Department, International Medical University)
Harun, Hazlan (Department of Chemistry, Universiti Sultan Zainal Abidin (UniSZA))
Kasim, Noor H. Abu (Faculty of Dentistry, Universiti of Malaya)
Alsalahi, Abdulsamad (Faculty of Pharmacy, Sana'a University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.10, 2012 , pp. 5131-5136 More about this Journal
Abstract
Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.
Keywords
Goniothalamin; HeLa cervical cancer cell line; fluorescence microscopy; cellular DNA content; apoptosis;
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