• Title/Summary/Keyword: Cutting media

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In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture (희귀 및 멸종위기 수종 개느삼의 액아배양을 통한 기내번식)

  • Moon, Heung-Kyu;Kim, Yong-Wook
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.229-234
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    • 2008
  • An efficient micropropagation was established by using axillary bud explants from two-year-old tree(Echinosphorea koreensis Nakai), which has been known as a rare and endangered species. Among various basal media tested, DKW medium was shown to be the best for axillary shoot elongation. The addition of both BA and TDZ to the medium induced 6 to 10 shoots per explant during eight weeks of culture, without showing any abnormal morphology at the shoot proliferation stage. However, high concentration of TDZ(>0.05 mg/L) appeared to cause hyperhydration on either leaf or shoot at the later developmental stage. Approximately 20% of shoots produced roots by the addition of 1.0 mg/L NAA but not by IBA($0.2{\sim}1.0$ mg/L). Ex vitro micro-cuttings were better source for root induction; up to 58.6% of the micro-cuttings rooted when 100 mg/L IBA was applied to the soil(vermiculite). More than 90% of plantlets with roots were successfully acclimatized and grew normally in the field. Therefore, we suggest that this endangered tree species can be effectively micropropagated by axillary bud culture system developed in this study.

Cutting Propagation of Eleutherococcus senticosus MAXIM (가시오가피의 거목번식방법(揷木繁殖方法))

  • Park, Ho-Ki;Park, Moon-Soo;Kim, Tai-Soo;Choi, In-Leok;Jang, Yeong-Sun;Kim, Gue-Seong
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.2
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    • pp.133-139
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    • 1994
  • Eleutherococcus senticocus MAXIM containing compounds for a cordial and lumbago is a native plant in this country. However it is generally difficult to propagate them by seed. This study was carried out to improve propagation efficacy by cutting method using Eleutherococcus senticosus MAXIM collected Mt. Odae for three years$('90{\sim}'92)$. Rooting was delayed by 3 to 12 days but callus formation and rooting percentage was high by 75%, 30% respectively in the treatment of Rooton-F powder, a chemical for rooting promotion, compared with non-treatment. Rooting percentage and rooting characters were not differenced by treatment for remove rooting inhibition compounds and soil-media mixured by 1:1 of vermiculite and perlite was suitable for elevating rooting ratio. A. chiisanensis and A. sieboldianum were rooted well in any time to be cut, while rooting activity of E. senticosus was different by time to be cut. Rooting ratio of E. senticosus was 26%, 36% and 60% when cutten March 20, July 20 and september 20, respectively.

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Effects of Pisolithus tinctorius Ectomycorrhizal Inoculation on in vitro Rooting of Tissue-Cultured Quercus acutissima Carr. and of Cutting of Pinus densiflora Sieb. et Zucc. (모래밭버섯 균근균(菌根菌)의 인공접종(人工接種)이 상수리나무 조직배양묘(組織培養苗)와 소나무 삽목묘(揷木苗)의 기내(器內) 발근(發根)과 생존(生存)에 미치는 영향(影響))

  • Lee, Kyung Joon;Kim, Jong Ju
    • Journal of Korean Society of Forest Science
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    • v.83 no.4
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    • pp.531-539
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    • 1994
  • We examined the in vitro rooting and survival of tissue cultured plantlets of Quercus acutissima Carr. and Pinus densiflora Sieb. et Zucc. after addition of Pisolithus tinctorius(Pt) ectomycorrhizal fungus inoculum to the medium and effects of three levels of sucrose and phosphorus in culture media. Shoots of Quercus acutissima were obtained from winter buds of a 30-year old tree and cuttings of Pinus densiflora from germinated seed, and they were inoculated with Pt in vitro. In both species, Pt enhanced shoot length, survival, number of adventitious roots, root length, and rooting percentage. Survival in Quercus acutissima was increased from 75% in control to 100% in Pt inoculation. Pt inoculation increased the percentage of rooting from 20% to 70% in Quercus acutissima cuttings and from 63% to 100% in Pinus densiflora cuttings. It is concluded that mycorrhizal inoculation to tissue cultured Quercus acutissima Carr. and to in vitro cutting of Pinus densiflora Sieb. et Zucc. has practical application to improvement of poor root development and initial period of reduced shoot growth in vitro.

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SINTERED $Al_{2}O_{3}$-TiC SUBSTRATE FOR THIN FILM MAGNETIC HEAD

  • Nakano, Osamu;Hirayama, Takasi
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 1998.04b
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    • pp.6-6
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    • 1998
  • In 1957, the first magnetic disk drive compatible with a movable head was introduced as an external file memory device for computer system. Since then, magnetic disks have been improved by increasing the recording density, which has brought about the development of a high performance thin film magnetic head. The thin film magnetic head has a magnetic circuit on a ceramic substrate using IC technology. The physical property of the substrate material is very important because it influences the tribology of head/disk interface and also manufacturing process of the head. $Al_{2}O_{3}$-TiC ceramics, so called ALTIC, is known to be one of the best substrate materials which satisfies this property requirement. Even though the head is not in direct contact with the disk, frequent instantaneous contacts are unavoidable due to its high rotating speed and the close gap between them. This may cause damage in the magnetic recording media and, thus, it is very important that the magnetic head has a good wear resistance. $Al_{2}O_{3}$-TiC ceramics has an excellent tribological property in head/disk interface. Manufacturing process of thin film head is similar to that of IC, which requires extremely smooth and flat surface of the substrate. The substrate must be readily sliced into the heads without chipping. $Al_{2}O_{3}$-TiC ceramics has excellent machineability and mechanical properties. $Al_{2}O_{3}$-TiC ceramics was first developed at Nippon Tungsten Co. as cutting tool materials in 1968, which was further developed to be used as the substrate materials for thin film head in collaboration with Sumitomo Special Metals Co., Ltd. in 1981. Today, we supply more than 60% of the substrates for thin film head market in the world. In this paper, we would like to present the sintering process of $Al_{2}O_{3}$-TiC ceramics and its property in detail.

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Zinc Inhibits Amyloid ${\beta}$ Production from Alzheimer's Amyloid Precursor Protein in SH-SY5Y Cells

  • Lee, Jin-U;Kim, Chul-Hoon;Kim, Dong-Goo;Ahn, Young-Soo
    • The Korean Journal of Physiology and Pharmacology
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    • v.13 no.3
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    • pp.195-200
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    • 2009
  • Zinc released from excited glutamatergic neurons accelerates amyloid ${\beta}$ (A ${\beta}$) aggregation, underscoring the therapeutic potential of zinc chelation for the treatment of Alzheimer's disease (AD). Zinc can also alter A ${\beta}$ concentration by affecting its degradation. In order to elucidate the possible role of zinc influx in secretase-processed A ${\beta}$ production, SH-SY5Y cells stably expressing amyloid precursor protein (APP) were treated with pyrrolidine dithiocarbamate (PDTC), a zinc ionophore, and the resultant changes in APP processing were examined. PDTC decreased A ${\beta}$ 40 and A ${\beta}$ 42 concentrations in culture media bathing APP-expressing SH-SY5Y cells. Measuring the levels of a series of C-terminal APP fragments generated by enzymatic cutting at different APP-cleavage sites showed that both ${\beta}$-and ${\alpha}$-cleavage of APP were inhibited by zinc influx. PDTC also interfered with the maturation of APP. PDTC, however, paradoxically increased the intracellular levels of A ${\beta}$ 40. These results indicate that inhibition of secretase-mediated APP cleavage accounts -at least in part- for zinc inhibition of A ${\beta}$ secretion.

The Type Classification on Modifiable Styling Methods in Wearing Folk Costumes (민속복식의 가변성 스타일링 유형분류)

  • Kim, Hye-Ree;Chun, Hei-Jung
    • Journal of the Korean Society of Costume
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    • v.61 no.8
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    • pp.114-126
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    • 2011
  • Many varieties in folk costumes have inspired fashion designs for a long time. Each country, with a unique style of its own reflects their own traditions, beliefs, customs and morals into their traditional costumes. The traditional methods of creating different ways of drapery methods by using only a rectangular piece of cloth have been passed down from generation to generation and it is still used today. This way of styling has been developed mainly in the Middle East, Asia and Africa and the modifiable ways of creating drapery wearing methods are as follows. The first modifiable styling method is changing the purpose of wearing costumes according to the needs and the wants of the person who wears it. The second method is exposing the knots on the surface of the garments and by doing this, there was no visible difference on the aesthetic formation and it did not alternate for a long time. This means that simple knot shapes have been used to tie edges of rectangular fabrics. The third method is a wrapping type of modifiable styling method, which has been used most commonly in folk costumes because there are lots of ways to wrap a rectangular cloth around the body. The fourth way is the fixed type of modifiable styling method and this method can only be noticed only in the Moroccan Izar using fibulas and belts to alter the shape of the Izar. The fifth method is the composite type of modifiable styling method. This method starts by cutting out cylindrical shapes of fabrics and then wraps or twists it to fit the body. The most significant character of modifiable styling methods in folk costumes is to use rectangular cloths, which varies only in ratio of length, width and sizes. Therefore, this study suggests that rectangular cloths can create enormous amounts of fashion styling which can be easily adapted and created in everyday fashion life.

Production of Plug Plantlets for Mass Propagation Using Stem Cuttings of Virus Free Microtubers in Potato (감자 바이러스 무균종묘의 대량생산과 플러그화에 관한 기초 연구)

  • 박양문;소인섭;유장걸;강봉균
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.678-686
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    • 1997
  • This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 2$0^{\circ}C$) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

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Gene Expression in Zn-deficient U937 Cell Line : Using cDNA Microarray (아연결핍된 단핵구 U937 Cell Line에 있어서의 유전자 발현 탐색 : cDNA Microarray 기법 이용)

  • Beattie, John H.;Trayhurn, Paul
    • Journal of Nutrition and Health
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    • v.35 no.10
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    • pp.1053-1059
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    • 2002
  • In post-genome period, the technique for identifying gene expression has been changed to high throughput screening. In the field of molecular nutrition, the need for this technique to clarify molecular function of the specific nutrient is essential. In this study, we have tested the zinc-regulated gene expression in zinc-deficient U937 cells, using cDNA microarray which is the cutting-edge technique to screen large numbers of gene expression simultaneously. The study result can be used for the preliminary gene screening data for clarifying, using monocyte U937 cell line, molecular Zn aspect in atherosclerosis. U937 cells were cultured in Zn-adequate (control, 12 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) ESMI media during 2 days, respectively. Cells were harvested and RNA was extracted. Total RNA was reverse-transcriptinized and synthesized cDNA probe labeled with Cy-3. fluorescent labeled cDNA probe was applied to microarray slide for hybridization slide, and after then, the slide was scanned using fluorescence scanner. ‘Highly expressed genes’ in Zn-deficient U937 cells, comparing to Zn-adequate group, are mainly about the genes for motility protein, immune system protein, oncogene and tumor suppressor and ‘Less highly expressed genes’ are about the genes for transcription, apoptosis associated protein, cell cycle, and several basic transcription factors. The results of this preliminary study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, specially Zn. Furthur study, using tailored-cDNA array and capillary endothelial cell lines, would be beneficial to clarify molecular Zn function, more in detail.

A Case study of engineering comprehensive design subject incorporating convergence with art (예술과의 융합을 접목한 공학 종합설계 교과목 사례연구)

  • Huh, Wonwhoi
    • Journal of the Korea Convergence Society
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    • v.13 no.4
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    • pp.195-201
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    • 2022
  • The core of the Fourth Industrial revolution, the topic of the 21st century, lies in the convergence of various technologies that are developing to the cutting edge. Therefore, convergence education is essential to solve problems from various perspectives. Above all, in order to solve the complex problem arising from the contact point between humans and technology in the future society, it was considered that insight through a humanities and artistic approach was necessary. Now, universities are trying to run a multidisciplinary comprehensive design to adapt to the new digital environment. In this study, an operation case of an engineering comprehensive design subject incorporating art operated by a researcher was described. The researcher presented an art convergence curriculum in a comprehensive design subject by applying 1. problem recognition and definition, 2. idea derivation and evaluation, 3. project development, 4. presentation, and demonstration process operation methods. Through this study, it is meaningful that it presented a method of operating art convergence in engineering comprehensive design subjects by allowing students to attempt an artistic aesthetic and emotional approach to project development.

A Study on XR Handball Sports for Individuals with Developmental Disabilities

  • Byong-Kwon Lee;Sang-Hwa Lee
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.6
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    • pp.31-38
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    • 2024
  • This study proposes a novel approach to enhancing the social inclusion and participation of individuals with developmental disabilities. Utilizing cutting-edge virtual reality (VR) technology, we designed and developed a metaverse simulator that enables individuals with developmental disabilities to safely and conveniently experience indoor handicapped handball sports. This simulator provides an environment where individuals with disabilities can experience and practice handball matches. For the modeling and animation of handball players, we employed advanced modeling and motion capture technologies to accurately replicate the movements required in handball matches. Additionally, we ported various training programs, including basic drills, penalty throws, and target games, onto XR (Extended Reality) devices. Through this research, we have explored the development of immersive assistive tools that enable individuals with developmental disabilities to more easily participate in activities that may be challenging in real-life scenarios. This is anticipated to broaden the scope of social participation for individuals with developmental disabilities and enhance their overall quality of life.