Browse > Article
http://dx.doi.org/10.5010/JPB.2008.35.3.229

In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture  

Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute)
Kim, Yong-Wook (Division of Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.35, no.3, 2008 , pp. 229-234 More about this Journal
Abstract
An efficient micropropagation was established by using axillary bud explants from two-year-old tree(Echinosphorea koreensis Nakai), which has been known as a rare and endangered species. Among various basal media tested, DKW medium was shown to be the best for axillary shoot elongation. The addition of both BA and TDZ to the medium induced 6 to 10 shoots per explant during eight weeks of culture, without showing any abnormal morphology at the shoot proliferation stage. However, high concentration of TDZ(>0.05 mg/L) appeared to cause hyperhydration on either leaf or shoot at the later developmental stage. Approximately 20% of shoots produced roots by the addition of 1.0 mg/L NAA but not by IBA($0.2{\sim}1.0$ mg/L). Ex vitro micro-cuttings were better source for root induction; up to 58.6% of the micro-cuttings rooted when 100 mg/L IBA was applied to the soil(vermiculite). More than 90% of plantlets with roots were successfully acclimatized and grew normally in the field. Therefore, we suggest that this endangered tree species can be effectively micropropagated by axillary bud culture system developed in this study.
Keywords
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Park SY, Ahn JK, Lee WY (2003) High frequency shoot induction from root segments of Albizzia coreana. J Korean For Soc 92: 626-631
2 Han MS, Moon HK, Kang YJ, Kim WW, Kang BS, Byun KO (2004) Micropropagation of an endangered species, Stellera rosea N. by tissue culture. Korean J Plant Biotechnol 31: 31-35   DOI   ScienceOn
3 Conde P, Sousa A, Costa A, Santos C (2008) A protocol for Ulmus minor Mill. Micropropagation and acclimatization. Plant Cell Tiss Org Cult 92: 113-119
4 Sanjaya, Muthan B, Rathore TS, Rai VR (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). J For Res 11: 203-209   DOI
5 Rai VR (2002) Rapid clonal propagation of Nothapodytes foetida (Wight) sleumer- a threatened medicinal tree. In Vitro Cell Dev Biol-Plant 38: 347-351   DOI   ScienceOn
6 Kim CS, Koh JG, Cho RM (1993) Effects of media growthregulators and dark treatment on in vitro propagation using vegetative buds of Prunus yedoensis M. Korean J Plant Tiss Cult 20: 213-219
7 Gomes GAC, Paiva R, Paiva PDO, Santiago EJAD (2003) Plant regeneration from callus cultures of Maclura tinctoria, an endangered woody species. In Vitro Cell Dev Biol-Plant 39: 293-295   DOI   ScienceOn
8 Lloyd G, McCown BH (1981) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Comb Proc Intl Plant Prop Soc 30: 421-427
9 Martin KP (2003) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21: 415-420   DOI
10 Lee YM, Lee WR (1997) Illustrated rare and endangered species in Korea. Korea Forest Research Institute, Jungbu Forest Experimental Station, pp 255
11 Youn Y, Lee SK, Park JI (1992) In vitro propagation of a rare species-Berchemia berchemiaefolia. Res Rep For Gen Res Inst Korea 28: 63-67
12 Moon HK, Suk GY, Kim SC (1997) MIcropropagation of a rare species, Forsythia saxatilis N. through tissue culture. J Korean For Soc 86: 430-434
13 Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum distichum Nakai. via axillary bud culture. Korean J Plant Tiss Cult 26: 133 -136
14 Shim KK, Ha YM, Son CJ, Han DS, Lee SA (2006) A study on development of materials for native landscape treenative area survey and characteristic of Korean endemic plant, Echinosophora koreensis Nakai. J Korea Tradition Landscape Architecture Soc 24: 32-42
15 Chen UC, Hsia CN, Yeh MS, Agrawal DC, Rsay HS (2006) In vitro micropropagation and ex vitro acclimation of Bulpleurum kaoi- an endangered medicinal plant native to Taiwan. In Vitro Cell Dev Biol-Plant 42: 128-133   DOI   ScienceOn
16 Debnath SC (2007) Influence of indole-3-butyric acid and propagation method on growth and development of in vitro and ex vitro-derived lowbush blueberry plants. Plant Growth Reg 51: 245-253   DOI
17 Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. HortSci 19: 507-509
18 Liesebach M, Naujoks G (2004) Approaches on vegetative propagation of difficult-to-root Salix caprea. Plant Cell Tiss Org Cult 79: 239-247   DOI
19 Beena MR, Martin KP (2003) In vitro propagation of the rare medicinal plant Ceropegia candelabrum L. through somatic embryogenesis. In Vitro Cell Dev Biol-Plant 39: 510-513   DOI   ScienceOn
20 Durkovic J (2006) Rapid micropropagation of mature wild cherry. Biol Plant 50: 733-736   DOI
21 Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1: 155-161   DOI
22 Kartsonas E, Papafotiou M (2007) Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tiss Org Cult 90: 111-116   DOI
23 Murashig T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-479   DOI
24 Negash L (2002) Successful vegetative propagation techniques for the threatened african pencil cedar (Juniperus procera Hoechst. ex Endl.). Forest Ecol. Manag 161: 53-64   DOI   ScienceOn