• 동의생리병리학회지
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• 제25권3호
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• pp.417-424
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• 2011

Ketoconazole (KET) is an antifungal drug with a broad spectrum of activity that also induces reproductive toxicity in humans and animals. KET inhibits C17-20 lyase which blocks the conversion of 17 ${\alpha}$-hydroxyprogesterone to androstenedione. The effect of Cuscutae semen(CS) extract against KET-induced testicular damage was evaluated in male rats. CS extract was administered orally (100 and 200 mg/kg) for 26 days. Three weeks after CS extract administration, KET was CS-administered intraperitoneally at a dose of 100 mg/kg once a day for 5 days. KET-induced reproductive toxicity was associated with clear reductions of the weights of testes and epididymides, sperm indices and serum testosterone levels. In addition, marked oxidative damage to testicular lipids and alterations of natural antioxidant enzymes were reported in association with KET toxicity. Most of the KET-induced effects were greatly decreased with the concomitant application of CS extract. This study suggests a protective role of Cuscutae semen extract that could be attributed to its antioxidant properties.

• 한국식품조리과학회지
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• 제24권1호
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• pp.46-51
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• 2008

본 연구에서는 토사자 에탄올 추출물의 항산화 효과와 항돌연변이 활성을 분석하였다. 토사자 에탄올 추출물은 100 ppm, 200 ppm의 농도에서 BHT보다 높은 항산화 활성을 나타내었다. 토사자 추출물은 본 연구에서 사용한 농도에서는 돌연변이를 유발하지 않았으며, 직접돌연변이원인 4-NQO에 의해 유발된 돌연변이를 90% 이상 억제시키는 효과를 보여주어 우수한 항돌연변이능을 가지고 있는 것으로 나타났다. 또한 토사자 에탄올 추출물은 폴리페놀 화합물을 다량 함유하고 있어 이들 항산화 물질이 토사자의 항산화 효과와 항돌연변이 활성에 영향을 주었을 것으로 사료되었다. 본 연구 결과 토사자 에탄올 추출물은 항산화 효과와 함께 돌연변이 유발을 억제하는 항돌연변이 활성을 가지는 것으로 나타나 천연항산화제 및 고부가가치의 기능성식품 신소재로서 이용될 가능성을 보여주었다.

• 생약학회지
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• 제19권3호
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• pp.193-200
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• 1988

Immunopotentiating activity of water extract of Lycii Fructus, Rubus Fructus, Torilis Fructus, Schizandrae Fructus and Cuscutae Semen were investigated using mouse fed with normal diet and protein deficient diet. Body weight, ratio of spleen or thymus to body weight, number of total peritoneal exudate cell, content of total serum protein and albumin content were decreased in the mouse fed with protein deficient diets as compared to the mouse fed with normal diet. Regardless of the nutritional condition, the supplement of the water extract of Lycii, Rubus Torilis, Schizandrae Fructus or Cuscutae Semen increased the ratio of spleen to body weight. The increment of this ratio was significant by the supplement of water extracts of Lycii and Torilis Fructus in mouse fed with protein deficient diet. The supplement of water extracts of Torilis Fructus to the mouse fed with protein deficient diet increased the content of total serum protein and albumin content significantly. Similar results were obtained with Lycii, Rubus, Schizandrae Fructus and Cuscutae Similar. The electrophorogram shows the difference in the concentration of some serum protein constituents between the mouse fed with normal diet and protein deficient diet.

• 대한한의학방제학회지
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• 제13권2호
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• pp.101-110
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• 2005

The present study was conducted to compare antioxidant activity of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH radical scavenging activity. The extract was studied using diphenyl-picryl-hydrazyl (DPPH) for DPPH method. DPPH radical scavenging activity was measured after. .10, 20 and 30 minutes. The extract was tested by 1, 5, 10, 50, 100, 500 and $1000{\mu}g/ml$ concentrations. The results showed that the extract scavenged DPPH radical with time-dependent manner. Also, the extract showed dose-dependent DPPH radical scavenging activity. The extract of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba scavenged DPPH radical with the IC50 being 2.7, 3.2, 2.9 and 1.1 mg/ml, respectively. In conclusion, the extract of Epimedii Herba, Cuscutae Semen, Cnidii Fructus and Psoraleae Fructus have antioxidant activity for the treatment of male sterility.

• 대한한방부인과학회지
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• 제27권2호
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• pp.12-22
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• 2014

Objectives: This study aimed to evaluate the effects of Cuscutae Semen water extract (CS) on MCF-7 human breast cancer cells. Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of $100{\mu}g/ml$, $300{\mu}g/ml$, $600{\mu}g/ml$ CS, gave it to MCF-7 cell. After these process we performed MTT assay to elucidate the ability of apoptosis. The result of mRNA was analyzed by RT-PCR. Results: Each of concentrated extracts CS decreased the survival rate of MCF-7 cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3, P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CS did not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make it more active but turn it on. Conclusions: According to the above results, we could suggest that CS can occur the apoptosis on MCF-7 cells.

• 한국식품영양과학회지
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• 제18권3호
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• pp.333-337
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• 1989

This study was carried out to investigate effects of Cuscutae semen extract(C. S. E) on $CCI_4$-induced liver damage in rabbits and acute toxicity in mice. $LD_{50}(mg/kg)$ of C. S. E. was 7.83g/kg by intraperitoneal administration in mice. C. S. E. groups were showed more rapid recuperation than the control group intoxicated by $CCI_4$ only. SGPT activity was lowered significantly in 800mg/kg after 6 days. The levels of total cholesterol and total bilirubin and alkaline phosphatase were decreased apparently in 800mg/kg from 6 days. It is suggested that C. S. E. can be administered not only as a therapeutic agent(such as liver tonics) but also a healthy food to shorten the recovery time in liver diseases.

• 대한한의학방제학회지
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• 제6권1호
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• pp.187-197
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• 1998

The study was aimed to investigate the effect cuscutae semen(CS) on the vascular systems including changes in blood pressure (BP), regional cerebral blood flow(rCBF) and pial arteriolar diameter of male Sprague-Dawely rats. The changes in rCBF were determinated by laser-Doppler flowmetry, and the changes in diameter of pial arteriole were measured through a closed crainal window. 1. Blood pressure was not affected by CS in rats. 2. rCBF was increased by CS in a dose-dependent manner. 3. Pretreatment with methylene blue(Img/kg), and propranolol(1mg/kg) significantly inhibited CS induced increased in rCBF. 4. Pretreatment with indomethacin(1mg/kg) did not inhibited CS induced increased in rCBF. 5. Pial arterial diameter was increased by CS in a dose-dependent manner. These results suggest that CS causes a diverse response of blood pressure, regional cerebral blood flow(rCBF), and pial arteral diameter. The increased in rCBF is also mediated by adrenergic ${\beta}-receptor $ and guanylate cyclase.

• 대한한방부인과학회지
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• 제25권4호
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• pp.1-11
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• 2012

Objectives: Osteoporosis, which occurs after menopause, is a kind of metabolic bone disorder. It develops when the bone mass begins to decrease radically, and its main symptoms are bone fracture and height-shortening. This thesis aims at what effects the Cuscutae Semen Extract(CSE) has on the prevention of osteoporosis in SD-rat that is caused by ovariectomy. Methods: The 24 female white rats, after removing their ovaries, were divided into the Normals, the Control group, and the CSE administrated group. For the next 8 weeks, distilled water to the normals and the control, and the CSE(45.9 mg/100 g) to the CSE administrated group were given in the mouths of them. After 8 weeks the rats were sacrificed. Weight, albumin, aspartate aminotransferase, alanine aminotransferase, total-cholesterol, triglyceride, phosphorus, calcium, tetraiodothyronine, estradiol, the weight of the femur, the amount of tibia ash, the area of trabecular bone, and the thickness of trabecular bone were measured. Results: The serum analysis shows that the calcium and phosphorous of the CSE administrated group significantly increased compared to that of the control group. Aspartate aminotransferase, alkalinephosphatase, total cholesterol, tetraiodothyronine of CSE group were decreased, but not so significant. Albumin, alanine aminotransferase, triglyceride and estradiol of the group showed the tendency to increase, but the significance wasn't admitted. Regarding the variation of bone, the femur weight and the ash content of tibia, CSE group was more increased than the control group but the significance wasn't admitted. The histological observation shows that the trabecular thickness was more increased than the control group, and trabecular area increased significantly compared to those of the control group. The number of osteoclast and osteoblast area of the CSE groups decreased significantly compared to those of the control group. Conclusions: From the result of the above study, CSE should be effective for the osteoporosis cure and precaution and deeper study through bedside and clinical demonstration is much needed from now on.

• 한국응용과학기술학회지
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• 제33권4호
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• pp.693-697
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• 2016

본 연구는 추출물 농도에 따른 생리활성물질의 용출량을 측정하기 위해 전자공여능과 총 폴리페놀 함량을 측정한 결과이다. 전자공여능은 추출물의 첨가 농도가 15%인 경우는 21.81%로 나타났고, 35% 농도에서 40.45%로 가장 높았다. 한약재의 첨가 농도가 증가함에 따라 전자공여능은 유의적으로 증가하였다(p<0.05). 가장 높은 35% 첨가 농도에서의 40.45% 공여능은 이보다 더 낮았으므로 전자공여능은 미약한 것으로 생각된다. 총 폴리페놀함량은 한방약술 15%에서는 $113.89{\pm}1.79{\mu}g\;GAE/m{\ell}$로 나타났고, 한방약술 35% 에서는 $274.24{\pm}0.71{\mu}g\;GAE/m{\ell}$로 나타나서 첨가물의 농도 증가에 따라 총 폴리페놀의 함량도 유의적으로 증가하였다(p<0.05). 추출물 농도가 30%에서 총 폴리페놀 함량의 증가 폭이 $61.75{\mu}g\;GAE/m{\ell}$로 가장 높았다.

• Journal of Acupuncture Research
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• 제32권3호
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• pp.69-81
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• 2015

Purpose : The first purpose of this study is to find out whether the water extract of Rehmanniae Radix Preparat(RRP), Cuscutae Semen(CS) and their combination(Ssangbohwan, SBH) have the effect of suppressing Receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation. The second purpose of this study is to find out whether the water extract of RRP, CS and SBH have the effect of inhibiting osteoporosis in an osteoporosis model induced by lipopolysaccharide(LPS). Methods : After promoting differentiation of osteoclasts by treating the RANKL, we observed the effect by the administration of RRP, CS and SBH. In addition, by means of Reverse transcription polymerase chain reaction(RT-PCR), we assayed mRNA expression levels of NFATc1, c-Fos, TRAP and GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, spermatogeni) from bone marrow macrophages(BMMs). Similarly, the protein expression levels of NFATc1 (Nuclear factor of activated T-cells, cytoplasmic1), C-Fos, MAPKs(Mitogen-activated protein kinases) and ${\beta}$-actin in cell lysates were analyzed by means of Western Blotting. Finally, we determined the anti-osteoporotic effects of RRP, CS and SBH, through the use of Lipopolysaccharide-induced bone-loss mouse. Results : RRP, CS and SBH showed remarkable inhibitive effect on RANKL-treated osteoclast differentiation without cytotoxicity. SBH inhibited the phosphorylation of p38, Jun N-terminal kinases(JNK), and I-${\kappa}B$ and down-regulated the induction of c-Fos and NFATc1 by RANKL. RRP, CS suppressed degradation of I-${\kappa}B$, but it did not affect c-Fos and NFATc1 by RANKL. Lastly, in vivo data showed that RRP and SBH prevented bone erosion by LPS treatment. Conclusions : These results demonstrate SBH can be effective remedy for bone-loss diseases such as osteoporosis.