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http://dx.doi.org/10.15204/jkobgy.2014.27.2.012

Effects of Cuscutae Semen Water Extract on Apoptosis of MCF-7 Human Breast Cancer Cells  

Kim, Ji-Hyun (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University, Graduate School, Dae-Jeon University)
Jung, Eun-Hye (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University, Graduate School, Dae-Jeon University)
Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University, Graduate School, Dae-Jeon University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.27, no.2, 2014 , pp. 12-22 More about this Journal
Abstract
Objectives: This study aimed to evaluate the effects of Cuscutae Semen water extract (CS) on MCF-7 human breast cancer cells. Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of $100{\mu}g/ml$, $300{\mu}g/ml$, $600{\mu}g/ml$ CS, gave it to MCF-7 cell. After these process we performed MTT assay to elucidate the ability of apoptosis. The result of mRNA was analyzed by RT-PCR. Results: Each of concentrated extracts CS decreased the survival rate of MCF-7 cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3, P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CS did not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make it more active but turn it on. Conclusions: According to the above results, we could suggest that CS can occur the apoptosis on MCF-7 cells.
Keywords
Cuscutae Semen; Breast Cancer; Apoptosis; MCF-7 Cell; Bcl-2; Bax; Caspase-3; P21; RIP-1;
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