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Effects of Normal Diet with or without Naringin Supplement Following Ethanol Diet on Changes in Lipid Profiles and Antioxidant Enzyme Activities in Rats

  • Seo, Hyun-Ju;Lee, Hyo-Sun;Choi, Myung-Sook
    • Nutritional Sciences
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    • v.9 no.2
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    • pp.82-91
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    • 2006
  • This study was performed to investigate the effect of normal diet with or without naringin supplement on the lipid and antioxidant metabolism in ethanol-treated rats for a short tenn. Male Sprague-Dawley rats were divided into three groups (n=10), which were assigned to one of three dietary categories : $E_8$ : ethanol diet for 8 wks, $E_4N_4$ : ethanol diet for the first 4 wks and normal diet for the last 4 wks, $E_4Nna_4$ : ethanol diet for the first 4 wks and normal diet with naringin supplement for the last 4 wks. Plasma total cholesterol concentrations were significantly higher in ethanol fed rats for 8 weeks. The HDL-C/total-C ratios of the $E_4N_4$ and the $E_4Nna_4$ groups were significantly higher than that of the $E_8$ group, while the atherogenic index was lower in the $E_4N_4$ and the $E_4Nna_4$ groups than in the $E_8$ group. The $E_4N_4$ and $E_4Nna_4$ diets significantly lowered both the hepatic cholesterol and triglyceride levels compared to the $E_8$ group. Accumulation of hepatic lipid droplets was observed to be the highest in the $E_8$ group. In the current study, the naringin supplement to normal diet significantly lowered both the hepatic HMG-CoA reductase and ACAT activities in ethanol pre-treated rats for 4 weeks. Antioxidant enzyme activities were also upregulated when ethanol feeding was ceased. Naringin supplement given for 4 weeks after ethanol cessation resulted in a significant decrease in the plasma cholesterol and hepatic lipids and plasma TBARS as well as the hepatic HMG-CoA reductase and ACAT activities compared to the rats given ethanol diet for the entire 8 weeks. Replacement of normal diet following a short tenn ethanol feeding was effective for the recovery of ethanol-induced fatty liver and for normalizing plasma and hepatic lipid profiles and antioxidant enzyme activities, regardless of an additional phytochemical supplement, naringin. The effect of naringin could seemingly be more evident if its supplementation period had been extended longer than 4 weeks after ethanol cessation.

Effects of Dietary Pearlzyme on Growth Performance and Development of Digestive Organs in Broilers (펄자임 첨가사료가 육계의 생산성과 소화기관의 발달에 미치는 영향)

  • Kim, Jimin;Kang, Seokmin;Yoon, Jeong Yong;Yang, Young-Rok;Kim, Won;Jang, Jung-Soon;Choi, Yang-Ho
    • Korean Journal of Poultry Science
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    • v.42 no.4
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    • pp.291-297
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    • 2015
  • We investigated the effects of dietary pearlzyme (mudflat-bacteria origin protease) on growth performance and development of digestive organs in broilers. Two hundred forty, 4 day-old female Ross broiler chicks were divided into 2 groups (control vs. pearlzyme) which were randomly housed in 8 pens with 15 chicks/pen. They were fed one of two diets containing pearlzyme at 0 or 0.1% for 4 weeks. Dietary pearlzyme resulted in significant increase in body weight during the first week of the experiment (p<0.05). With age, weight gain and feed efficiency continued to decrease reaching significant level during the last week. Mortality was 3.3% in control but not in pearlzyme during the entire period of the experiment. Dietary pearlzyme resulted in increased weight (p<0.05) in the ceca and rectum, and increased length in the ceca (p<0.05). However, there were tendencies to increase the weight of the gizzard (p<0.071) but to decrease the length of the small intestine (p<0.068). The results of the current study show that dietary pearlzyme affects weight gain and the development of digestive organs.

Applicability Evaluation of Nile Blue A Dye to Korean Termite (Reticulitermes speratus kyushuensis) (국내 서식 흰개미의 개체 및 군체 식별을 위한 Nile Blue A 염색법 적용성 평가)

  • Kim, Si Hyun;Im, Ik Gyun;Chung, Yong Jae
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.333-338
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    • 2020
  • Dye markers are used in the study of subterranean termites to determine colony territories and population sizes. Dyes not only allow the study of termite biology, but can also be used for control purposes. Although Reticulitermes speratus kyushuensis causes damage to wooden historical properties in Korea, the dye markers have not been applied. Therefore, we evaluated the applicability of Nile Blue A dye for R. s. kyushuensis. Filter papers dyed with concentrations of 0.05%, 0.10%, and 0.25% (w/w) were each fed to termite populations through a no-choice feeding test for 1, 3, or 7 days. After 4 weeks, the survival rate, dyeing rate, and maintenance of dyeing were recorded. The results showed high survival rates with lower dye concentrations and shorter feeding periods. Among the combinations of concentrations and days, high survival rates, dyeing rates, and dyeing maintenance periods were found at concentrations of 0.05% and 0.10% for 3 days of feeding. These maximized the survivorship and visibility. The results from the current study can be applied to investigate the ecology and control of R. s. kyushuensis in both the laboratory and field.

Growth Performance and Nutrient Composition in the White-spotted Flower Chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae) Fed Agricultural By-product, Soybean Curd Cake (비지박 첨가 먹이원 급여에 따른 흰점박이꽃무지 유충의 생육과 영양성분 변화)

  • Song, Myung-Ha;Han, Moon-Hee;Lee, Seokhyun;Kim, Eun-Sun;Park, Kwan-Ho;Kim, Won-Tae;Choi, Ji-Young
    • Journal of Life Science
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    • v.27 no.10
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    • pp.1185-1190
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    • 2017
  • Insects are gaining recognition as an alternative source of protein. As a result, more and more domestic farms have begun mass rearing of edible insects. In order to produce high quality insects, studies on the development of safe and nutritious feed sources are needed. Given the cost of rearing insects, agricultural and industrial by-products are good sources for feed. The efficient utilization of these by-products can help in reducing the cost of production and preventing environmental pollution. In the current study, Citrus unshiu peel (CP), soybean curd cake (SCC), soybean oil meal (SOM), and brewers dried grain (BDG) were investigated for their effects on larval growth and development of Protaetia brevitarsis. Interestingly, the feed with 10% SCC increased larval weight by 3.5 times. For the larval period, the group of 10% SCC was significantly shorter than the control. Furthermore, minerals such as Zn, Ca, K, Mg, Na, and P were recorded to be high in 10% SCC. A total of 17 amino acids were present in 10% SCC, of which tyrosine and arginine were predominant. The heavy metal contents were very small amounts or not detected in any of the investigated groups. These findings provided a scientific basis for the utilization of soybean curd cake as a nutritional feed source to promote larval growth and produce quality insects.

Dietary chia (Salvia hispanica L.) improves the nutritional quality of broiler meat

  • Mendonca, Nicole Batelli de Souza Nardelli;Filho, Sergio Turra Sobrane;de Oliveira, David Henrique;Lima, Eduardo Machado Costa;e Rosa, Priscila Vieira;Faria, Peter Bitencourt;Naves, Luciana de Paula;Rodrigues, Paulo Borges
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.8
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    • pp.1310-1322
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    • 2020
  • Objective: The current study was conducted to evaluate the quality and profile of fatty acid in the breast and thigh, and the performance of broilers fed diets containing seed or oil of chia (Salvia hispanica L.) as a replacement for soybean, in the rearing period from 29 to 42 days of age. Methods: On the 29th day of age, 120 broilers were distributed in four treatments evaluated in five replicates of six birds. The grain or oil of soybean was respectively replaced on a weight-to-weight basis in the formulation by the seed or oil of chia, constituting the experimental diets. The roasted whole soybean and chia seed were included in the feed at 16.4%, whereas the soybean and chia oils were included at 2.5%. Results: The dietary chia oil increased the lipid peroxidation in the thigh meat, and the dietary chia seed increased the cooking loss of the thigh. However, for the other physicochemical parameters evaluated and for the proximate composition of the breast and thigh, in general, the inclusion of chia seed or oil in the diet provided similar or better results than those observed when the diets contained soybean oil or roasted whole soybean. With regard to the fatty acid profile and associated parameters, dietary chia increased the concentrations of α-linolenic, eicosapentaenoic, and docosahexaenoic acids and reduced the Σω-6:Σω-3 ratio and the atherogenicity and thrombogenicity indices of the broiler meat. However, the dietary chia seed worsened the feed conversion ratio. Conclusion: Diet containing 2.5% chia oil supplied to broilers during the period from 29 to 42 days of age improves the feed conversion ratio, increases the deposition of the ω-3 fatty acids in the breast and thigh, in addition to reducing the Σω-6:Σω-3 ratio and the atherogenicity and thrombogenicity indices, thereby resulting in meat with higher nutritional quality.

A Preliminary Development of Real-Time Hardware-in-the-Loop Simulation Testbed for the Satellite Formation Flying Navigation and Orbit Control (편대비행위성의 항법 및 궤도제어를 위한 실시간 Hardware-In-the-Loop 시뮬레이션 테스트베드 초기 설계)

  • Park, Jae-Ik;Park, Han-Earl;Shim, Sun-Hwa;Park, Sang-Young;Choi, Kyu-Hong
    • Journal of Astronomy and Space Sciences
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    • v.26 no.1
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    • pp.99-110
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    • 2009
  • The main purpose of the current research is to developments a real-time Hardware In-the-Loop (HIL) simulation testbed for the satellite formation flying navigation and orbit control. The HIL simulation testbed is integrated for demonstrations and evaluations of navigation and orbit control algorithms. The HIL simulation testbed is composed of Environment computer, GPS simulator, Flight computer and Visualization computer system. GPS measurements are generated by a SPIRENT GSS6560 multi-channel RF simulator to produce pseudorange, carrier phase measurements. The measurement date are transferred to Satrec Intiative space borne GPS receiver and exchanged by the flight computer system and subsequently processed in a navigation filter to generate relative or absolute state estimates. These results are fed into control algorithm to generate orbit controls required to maintain the formation. These maneuvers are informed to environment computer system to build a close simulation loop. In this paper, the overall design of the HIL simulation testbed for the satellite formation flying navigation and control is presented. Each component of the testbed is then described. Finally, a LEO formation navigation and control simulation is demonstrated by using virtual scenario.

Feeding Behavior of the Small Brown Planthopper, Laodelphax striatellus (Hemiptera: Delphacidae) on Rice Plants Based on EPG Waveform, Honeydew Excretion, and Microsection Analysis (EPG 파형과 감로 분비, 미세절편 관찰로 해석된 애멸구의 벼 섭식행동)

  • Seo, Bo Yoon;Kwon, Youn-Hee;Jung, Jin Kyo;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.351-358
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    • 2016
  • Consistent with a previous study on the brown planthopper Nilaparvata lugens (BPH) (Seo et al., 2009), we identified seven distinct EPG waveforms (np, L1, L2, L3, L4-a, L4-b, and L5) in adult female Laodelphax striatellus (SBPH) that fed on rice plants, by using the direct current electrical penetration graph (DC-EPG) system. The shape of waveforms and the pattern of occurrence of each waveform of SBPH were very similar to those of BPH. L3 and L4-a always occurred prior to L4-b. Periodical honeydew excretion was observed in L4-b only. Microsection observation following laser stylectomy revealed that the tips of SBPH stylets severed in L3, L4-a, and L4-b were commonly located in or near the phloem region of rice plants, but were located in the xylem in L5. Plant sap flowed from the stylets severed in L4-b only, and its main carbohydrate component was detected as sucrose by HPLC analysis. These results and the patterns of EPG waveform progress in SBPH suggested that feeding activities on rice plant tissue were relevant to each EPG waveform. L1 and L2 corresponded to the initiation of stylet penetration and stylet movement with salivation on the outside of the vascular bundle. L3 and L4-a were related to feeding activities within the phloem region in preparation for phloem sap ingestion. L4-b was closely associated with phloem sap ingestion, and L5 corresponded to xylem feeding behavior.

Supplementation of Safflower Seed Powder and Extracts Enhances Bone Metabolism in Rib-Fractured Rats

  • Seo, Hyun-Ju;Moon, Kwang-Deog;Jeon, Seon-Min;Kim, Jun-Han;Cho, Myung-Sook
    • Preventive Nutrition and Food Science
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    • v.8 no.1
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    • pp.46-53
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    • 2003
  • The current study investigated the effect of Korean safflower (Carthamus tinctorius L.) seed powder and its water and ethanol extracts on bone metabolism during recovery from rib-fracture induced by surgical operation in rats. 10-week-old male Sprague-Dawley rats weighing about 320 g were divided into 9 groups after arrival: 10d control (AIN 76 semi-purified diet), 10d safflower seed powder (10d SS-powder), 10d safflower seed ethanol extract (10d SS-EtOH), 10d safflower seed water extract (10d SS-$H_2O$), 20d control (AIN-76 semi-purified diet), 20d safflower heed powder (20d SS-powder), 20d safflower seed ethanol extract (20d SS-EtOH), 20d safflower seed water extract (20d SS-$H_2O$), and 20d sham-operation (20d sham), The dietary level for all the supplements was 5% based on the raw material weight. The rats were fed the experimental diets for 10 days before the rib fracture operation and for a further 10 or 20 days after the operation. A number 9 rib was fractured surgically and a sham-operation also performed. The rats were then sacrificed on the l0th or 20th day after the operation. The body weight initially decreased after the operation in all the rib-fractured groups, then gradually recovered. The concentrations of plasma osteocalcin were higher in the control group than in all the safflower-supplemented groups 10 and 20 days after the rib-fracture (p < 0.05). The bone-specific ALP (alkaline phosphatase) activity was significantly higher in the SS-EtOH group than in the other groups 20 days after the rib-fracture (p < 0.05). The level of urinary DPD (deoxypridinoline) was significantly higher in the SS-EtOH and SS-$H_2O$ groups than in the other groups 10 days after the rib-fracture. When comparing the PTH (parathyroid hormone) and calcitonin levels, the SS-$H_2O$ group exhibited the highest PTH level among the groups 10 and 20 days after the rib-fracture. Thus, it was concluded that the bone turnover during the fracture-healing period was more rapid in the rats supplemented with safflower seed powder or its fractions than in the control rats. Furthermore, the SS-$H_2O$ fraction was identified as the most effective in stimulating bone remodeling, as bone resorption and bone formation were both significantly increased during fracture healing when compared to the control group.

Effects of Dietary Peat Moss on Performance in Broilers (피트모스 첨가사료가 육계의 생산성에 미치는 영향)

  • Kim, Jimin;Kang, Seokmin;Yang, Young-Rok;Yoon, Jeong-Yong;Jo, Kwonho;Choi, Yang-Ho
    • Korean Journal of Poultry Science
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    • v.42 no.3
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    • pp.267-274
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    • 2015
  • Peat moss has been found to be useful as a plant growth substance, fertilizer, and soil solution. It is not known, however, whether dietary peat moss affects performance in broilers. In the present study, we investigated the effects of dietary peat moss as a feed ingredient in broilers. Experimental diets were prepared by mixing basal feed with PM at 0.0, 0.2, 0.4, 0.8, and 1.6%. In experiment 1, 150 14-day-old Ross broiler chicks were housed for 2 weeks in 15 pens that were randomly assigned into five groups (0.0%, 0.2%, 0.4%, 0.8% and 1.6% PM). Dietary peat moss at 0.2% and 0.4% significantly increased the water intake, body weight, weight gain and feed efficiency compared with the control (0.0% PM, P<0.05) but did not affect feed intake. Higher levels of PM (0.8 and 1.6%) significantly decreased feed intake, body weight and weight gain. In experiment 2, 198 7-day-old Ross chicks were housed for 3 weeks in nine pens that were randomly assigned into three groups (0.0%, 0.2% and 0.4% PM). When the broilers were fed with dietary peat moss for 3 weeks, the peat moss effects disappeared. The results of the current study show that low amounts of dietary peat moss may exert beneficial effects on performance in broilers.

Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs (친환경 복합효소제 첨가가 이유자돈의 성장, 장내 형태학, 영양소 소화율에 미치는 영향)

  • Kim, Seong-Ki;Cho, Myung-Woo;Kim, Jun-Su;Jang, Ki-Beom;Kim, Sheen-A;Mun, Da-Ye;Kim, Byeong-Hyeon;Kim, Young-Hwa;Park, Jun-Cheol;Choe, Jee-Hwan;Song, Min-Ho
    • Korean Journal of Organic Agriculture
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    • v.26 no.1
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    • pp.141-149
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    • 2018
  • This experiment was conducted to investigate the effects of multi-enzyme on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multi-enzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Measurements were growth performance, morphology of ileum, apparent ileal digestibility and apparent total tract digestibility of dry matter, crude protein, and energy of weaned pigs. There were no significant differences on growth performance during overall experimental period. No differences were found for the morphology of ileum and nutrient digestibility between CON and Multi groups. Therefore, the results in the current study indicated that multi-enzyme supplementation in diets had no effects on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.