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http://dx.doi.org/10.5656/KSAE.2016.07.0.039

Feeding Behavior of the Small Brown Planthopper, Laodelphax striatellus (Hemiptera: Delphacidae) on Rice Plants Based on EPG Waveform, Honeydew Excretion, and Microsection Analysis  

Seo, Bo Yoon (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kwon, Youn-Hee (Department of Plant Medicine, Chungbuk National University)
Jung, Jin Kyo (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Kim, Gil-Hah (Department of Plant Medicine, Chungbuk National University)
Publication Information
Korean journal of applied entomology / v.55, no.4, 2016 , pp. 351-358 More about this Journal
Abstract
Consistent with a previous study on the brown planthopper Nilaparvata lugens (BPH) (Seo et al., 2009), we identified seven distinct EPG waveforms (np, L1, L2, L3, L4-a, L4-b, and L5) in adult female Laodelphax striatellus (SBPH) that fed on rice plants, by using the direct current electrical penetration graph (DC-EPG) system. The shape of waveforms and the pattern of occurrence of each waveform of SBPH were very similar to those of BPH. L3 and L4-a always occurred prior to L4-b. Periodical honeydew excretion was observed in L4-b only. Microsection observation following laser stylectomy revealed that the tips of SBPH stylets severed in L3, L4-a, and L4-b were commonly located in or near the phloem region of rice plants, but were located in the xylem in L5. Plant sap flowed from the stylets severed in L4-b only, and its main carbohydrate component was detected as sucrose by HPLC analysis. These results and the patterns of EPG waveform progress in SBPH suggested that feeding activities on rice plant tissue were relevant to each EPG waveform. L1 and L2 corresponded to the initiation of stylet penetration and stylet movement with salivation on the outside of the vascular bundle. L3 and L4-a were related to feeding activities within the phloem region in preparation for phloem sap ingestion. L4-b was closely associated with phloem sap ingestion, and L5 corresponded to xylem feeding behavior.
Keywords
Laodelphax striatellus; EPG waveform; Feeding behavior; Honeydew excretion; Microsection;
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Times Cited By KSCI : 4  (Citation Analysis)
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