Journal of Conservation Science (보존과학회지)

The Korean Society Of Conservation Science For Cultural Heritage (한국문화재보존과학회)
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Applicability Evaluation of Nile Blue A Dye to Korean Termite (Reticulitermes speratus kyushuensis)

국내 서식 흰개미의 개체 및 군체 식별을 위한 Nile Blue A 염색법 적용성 평가

  • Kim, Si Hyun (Safety and Disaster Prevention Division, National Research Institute of Cultural Heritage) ;
  • Im, Ik Gyun (Department of Cultural Heritage Science, Chungbuk National University) ;
  • Chung, Yong Jae (Department of Heritage Conservation and Restoration, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage)
  • 김시현 (국립문화재연구소 안전방재연구실) ;
  • 임익균 (충북대학교 대학원 문화재과학협동과정) ;
  • 정용재 (한국전통문화대학교 문화유산전문대학원 문화재수리기술학과)
  • Received : 2020.08.18
  • Accepted : 2020.09.24
  • Published : 2020.10.20
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Abstract

Dye markers are used in the study of subterranean termites to determine colony territories and population sizes. Dyes not only allow the study of termite biology, but can also be used for control purposes. Although Reticulitermes speratus kyushuensis causes damage to wooden historical properties in Korea, the dye markers have not been applied. Therefore, we evaluated the applicability of Nile Blue A dye for R. s. kyushuensis. Filter papers dyed with concentrations of 0.05%, 0.10%, and 0.25% (w/w) were each fed to termite populations through a no-choice feeding test for 1, 3, or 7 days. After 4 weeks, the survival rate, dyeing rate, and maintenance of dyeing were recorded. The results showed high survival rates with lower dye concentrations and shorter feeding periods. Among the combinations of concentrations and days, high survival rates, dyeing rates, and dyeing maintenance periods were found at concentrations of 0.05% and 0.10% for 3 days of feeding. These maximized the survivorship and visibility. The results from the current study can be applied to investigate the ecology and control of R. s. kyushuensis in both the laboratory and field.

이 연구는 국외에서 지중흰개미의 생태와 방제 연구를 위해 사용하는 Nile Blue A 염색약이 국내 전역에 서식하는 R. s. kyushuensis에 대한 적용 여부 및 적정 조건을 평가하기 위해 수행되었다. 0.05%, 0.10%, 0.25%(w/w) 농도로 여과지를 염색한 뒤 1, 3, 7일간 no-choice feeding test로 염색 여과지를 강제 섭식시켜 공시충을 염색시킨 뒤 4주 동안 생존률, 염색률, 염색의 유지 여부 등을 관찰하였다. 그 결과 공시충의 생존률은 약제의 농도가 낮을수록, 약제의 섭식기간이 짧을수록 높은 경향을 보였으며 반대로 염색률 및 선명도는 약제의 농도가 높고 약제의 섭식기간이 길수록 높게 나타나 반비례하는 양상을 보였다. 다양한 농도와 섭식기간 조건 중 0.05%에서 3일, 0.10% 농도에서 3일간 염색하였을 때 높은 생존률과 염색률, 염색의 유지기간을 동시에 만족시켜 적합한 조건으로 확인되었다. 본 실험 결과는 향후 실내 실험 단계의 흰개미 관련 연구에서 약제의 섭식 여부 확인 등에 활용할 수 있을 것으로 기대된다.

Keywords

References

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