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http://dx.doi.org/10.12654/JCS.2020.36.5.04

Applicability Evaluation of Nile Blue A Dye to Korean Termite (Reticulitermes speratus kyushuensis)  

Kim, Si Hyun (Safety and Disaster Prevention Division, National Research Institute of Cultural Heritage)
Im, Ik Gyun (Department of Cultural Heritage Science, Chungbuk National University)
Chung, Yong Jae (Department of Heritage Conservation and Restoration, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage)
Publication Information
Journal of Conservation Science / v.36, no.5, 2020 , pp. 333-338 More about this Journal
Abstract
Dye markers are used in the study of subterranean termites to determine colony territories and population sizes. Dyes not only allow the study of termite biology, but can also be used for control purposes. Although Reticulitermes speratus kyushuensis causes damage to wooden historical properties in Korea, the dye markers have not been applied. Therefore, we evaluated the applicability of Nile Blue A dye for R. s. kyushuensis. Filter papers dyed with concentrations of 0.05%, 0.10%, and 0.25% (w/w) were each fed to termite populations through a no-choice feeding test for 1, 3, or 7 days. After 4 weeks, the survival rate, dyeing rate, and maintenance of dyeing were recorded. The results showed high survival rates with lower dye concentrations and shorter feeding periods. Among the combinations of concentrations and days, high survival rates, dyeing rates, and dyeing maintenance periods were found at concentrations of 0.05% and 0.10% for 3 days of feeding. These maximized the survivorship and visibility. The results from the current study can be applied to investigate the ecology and control of R. s. kyushuensis in both the laboratory and field.
Keywords
Termite; Dye; Nile Blue A; Reticulitermes speratus kyushuensis;
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