• Animal Systematics, Evolution and Diversity
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• 제7권2호
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• pp.233-240
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• 1991

중국 칭다오(북위 36도 08분, 동경 120도 43분)의 새우양식장에서 채집된 해산 하모섬모충류가 Oxytricha saltans(Cohn,1866)로 밝혀져 형태 및 생태에 관한 연구를 실시했다. 서식처에서 채집한 표본과 실험실에서 배양한 것을 각각 생체 관찰하고 protargol로 염색하여 섬모하부구조를 관찰했다. 크기가 작아 분류학적 연구가 미흡했던 본 종에 대해서 새로운 식별기재를 제시했고 상세히 재기재 했으면 근연종 Actinotricha saltans sensu Dragesco, 1963에 관해 논의했다.

• 수산해양교육연구
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• 제28권6호
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• pp.1724-1738
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• 2016

This paper was researched on economic feasibility and sensibility of operation to offshore cage culture for cod. Offshore cage culture for cod needs to invest high budget what to construction and operation. And it was required variety methods about analysis of economic feasibility. Therefore, these were studied NPV, B/C and sensitivity for each by assuming a six scenarios considering the product performance according to the size of cod and culturing methods of fingerlings, etc. As a results, even though economy, if efficiency is low, it is a priority need the technical development to promote the feed efficiency to increase economic feasibility and should make efforts to enhance the business economy to strengthen the price competitiveness pricing with high quality products through quality control and brand recognition of cod. It expects to be used as a reference for related research in the future by deriving policy implications based on the method of analysis of the economic feasibility on offshore cage culture for cod.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2000년도 추계학술발표대회
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• pp.85-91
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• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. It secrets large quantities of proteins or enzymes into the culture medium which makes this organism appealing for the production of heterologous proteins. Recently Electric field-mediated transformation method, electroporation, has been applied to fungal transformation. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, decreasing the culturing time and non-protoplast transformation for the increment of transformation efficiency. Transformants were obtained with electroporation in optimal condition 2,500 voltage, 1,540 ohm and 0.50 capacitance. More than 1,000 transform ants were obtained with 6-10 hrs cultured mycelia without enzyme treatment, called non-protoplast transformation.

• 한국해양생명과학회지
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• 제1권1호
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• pp.70-78
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• 2016

Microalgae are of significant importance for future biotechnological applications. Many microalgae banks or laboratories attempt to maintain various microalgae for further research purposes. Cryopreservation has been preferred to reduce a labor-intensive and costly routine sub-culturing. Cryopreservation can also diminish the genetic drift risk. However, cryopreservation as a long term storage of microalgae method are still in developing progress because it cannot be generalized for all microalgae. Microalgae types, cryoprotectant agents (CPAs) types, freezing and thawing methods are the most important factors that should be considered for cryopreservation. In this short review the basic principles and the current advanced of microalgae cryopreservation methods are discussed with a suggested starting parameters for microalgae cryopreservation.

• 한국기계가공학회지
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• 제13권3호
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• pp.35-41
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• 2014

Poly(g-glutamic acid) (g-PGA) is a very promising biodegradable polymer that is produced by microorganism of Bacillus subtilis. Because g-PGA is water-soluble, anionic, biodegradable, and even edible, its potential applications have been studied from an industrial standpoint. In this study, we fabricated porous g-PGA foams by means of a freeze-solvent extraction method for tissue-engineering applications. Porous g-PGA foams were chemically cross-linked using a hexamethylene diisocyanate solution. An aqueous basic solution was used to neutralize g-PGA foam for cell culturing. During an in vitro cell culture study, it was observed that primary rabbit ear chondrocytes were well at tached and spread over the surface oft hree-dimensional cross-linkedg-PGA foam. From these results, it is concluded that cross-linkedg-PGA foam is aprom is in gmaterial for tissue-engineering applications, especially those pertaining to the regeneration of human cartilage.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2007년도 학술발표회
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• pp.27-31
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• 2007

This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factors such as TNF-${\alpha}$ and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which showed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR. Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors (NK. cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

• 식품저장과 가공산업
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• 제6권2호
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• pp.11-13
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• 2007

This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factor such as TNF-a and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which shoed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR, Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors(NK cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

• Restorative Dentistry and Endodontics
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• 제41권4호
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• pp.283-295
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• 2016

Objectives: In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. Materials and Methods: HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting. Results: Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups. Conclusions: Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs.

• 대한의용생체공학회:의공학회지
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• 제38권2호
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• pp.62-73
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• 2017

To identify a solution for the restricted availability of healthy lungs and the high risk of immune rejections following organ transplantation, tissue engineering techniques for culturing lungs have been studied by many research groups. The most promising method for culturing lungs is the utilization of a bio-scaffold that was prepared using harvested organs from human donors or other animals by removing their original cells. In this study, a pulsatile perfusion pump was used to alleviate the cell removal effect with the high fluid-dynamic power of the perfusion stream during the decellularization process, while other conventional studies focused on chemical methods to identify efficient detergents. The purpose of this study was to analyze the developed device by using energy equivalent pressure (EEP), which is an indicator of pulsatility, to understand the characteristics of pulsatile energy transmitted according to the load size by using the artificial model and compare it with the measured EEP. The pulsatility of the device can be estimated with the concept of fluid-dynamic energy during a particular constant time period or fluid-dynamic power represented as EEP and EEP increment. Because the measured EEP of perfusion flow during decellularization can be changed by the amount of fluid leakage and the degree of clogging in the capillary vessels, EEP should be measured to determine whether the decellularization is progressing without problems. The decrement of EEP caused by the high perfusion resistance was observed from some experimental results that were obtained with artificial models. EEP can be used to monitor the decellularization process after analyzing the varying EEP according to the amount of load. It was confirmed that the EEP was maintained at a high level in the experiment using the harvested lungs from 12-13-week-old rats. In addition, it was confirmed that the cell removal time was faster than when continuous perfusion was performed. In this study, pulsatile power delivered to the lungs was measured to monitor the process of cell removal, and it serve as the evidence for efficient decellularization.

• 한국양식학회지
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• 제10권2호
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• pp.105-112
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• 1997

동해안에서 고품질의 굴 양식 생산과 어업인의 안정적인 소득 향상을 도모하고저 경남 하동에서 채묘한 굴을 강원도 주문진 연안에 연승수하식으로 이식한 후 1994년 8월부터 1995년 7월까지 어장환경과 수층별 성장시험을 실시하였다. 환경요인 조사결과 수온은 8.33-$25.62^{\circ}C,\;염분\;32.84-34.28\textperthousand,\;영양염류인\;인산염은\;0.09-0.40\mug$-at/l, 용존성 무기질소(DIN)는 0.32-3.12$\mu$g-at/l로 나타났다. 굴의 성장은 상층이 각고 88.7 mm, 중층 84.9 mm, 하층 78.0 mm로 하층에 비해 상층의 성장이 더 빨랐다. 수층별 각고(SH)에 대한 각장(SL)의 상대성장식은 상층 SL=0.5403 SH+8.5486 (r=0.9959), 중층 SL=0.5813 SH+3.7775 (r=0.9869), 하층 SL=0.5159 SH+6.8736 (r=0.9961) 이였다. 육질은 상층 13.24 g, 중층 12.68 g, 하층 10.96 g으로 상층에서 높게 나타났으며, 수층별 전중량(TW)에 대한 육중량(MW)의 상대성장식은 상층 MW=0.1933 TW+0.1051 (r=0.9973), 중층 MW=0.1915 TW+0.1894 (r=0.9984), 하층 MW=0.1650 TW+0.0558 (r=0.9983)으로 나타났다.