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Current Advances in Cryopreservation of Microalgae

  • Nugroho, Wahyu Sri Kunto (Department of Chemistry, Pukyong National University) ;
  • Kim, Do-A (Department of Chemistry, Pukyong National University) ;
  • Kim, Dong-Woo (Department of Chemistry, Pukyong National University) ;
  • Koo, Bon-Won (Department of Chemistry, Pukyong National University) ;
  • Hur, Young Baek (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Hak Jun (Department of Chemistry, Pukyong National University)
  • Received : 2016.04.05
  • Accepted : 2016.04.25
  • Published : 2016.04.30

Abstract

Microalgae are of significant importance for future biotechnological applications. Many microalgae banks or laboratories attempt to maintain various microalgae for further research purposes. Cryopreservation has been preferred to reduce a labor-intensive and costly routine sub-culturing. Cryopreservation can also diminish the genetic drift risk. However, cryopreservation as a long term storage of microalgae method are still in developing progress because it cannot be generalized for all microalgae. Microalgae types, cryoprotectant agents (CPAs) types, freezing and thawing methods are the most important factors that should be considered for cryopreservation. In this short review the basic principles and the current advanced of microalgae cryopreservation methods are discussed with a suggested starting parameters for microalgae cryopreservation.

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