• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2003년도 추계 학술발표회
• /
• pp.60-60
• /
• 2003

• Journal of Plant Biotechnology
• /
• 제2권1호
• /
• pp.29-33
• /
• 2000

To develop an efficient propagation method for yew tree, zygotic embryos were cultured under various conditions. When dissected embryos were cultured on GA$_3$ containing media, the highest germination frequency was observed on WPM medium contaning 1.0 mg/L GA$_3$. For germination of the embryos, two different conditions were compared; culturing embryos with endosperm (Method I), and 2) culturing embryos only (Method II). Maximum germination was achieved in 0.5 mg/L GA$_3$ when embryos with endosperm were cultured on the media. Of the media tested, White and WPM medium were the most suitable on germination of embryos. The abnormality of yew embryos found was observed when it cultured on GA$_3$ or culture media. About 40% of the precociously germinated embryos could be developed into full seedlings. Seedlings contained taxol in high quantity (535 $\mu\textrm{g}$/g dry weight). In vitro techniques will be sewed as a useful tool for the development of transformed root cultures and biosynthesis studies.

• Journal of Ginseng Research
• /
• 제9권1호
• /
• pp.42-53
• /
• 1985

This study was carried out to obtain the basic information about the large scale propagation of ginseng (Panax ginseng C.A. Meyer). Therefore, the stem cuttings of 1-year old ginseng, treated with various concentrations of plant growth regulators for 5 seconds (quick dipping) and 24 hours (prolonged soaking), were cultured. The root formation of stem cuttings was varied with the concentrations, kinds, and treatment methods of plant growth regulators. Besides normal-looking roots various malformed roots were observed. In the prolonged soaking method, the culture of stem cuttings, treated with 10 ppm of IBA or NAA, resulted in profuse root regeneration. And stem cuttings, in quick dipping method, treated with 2000 ppm of IBA or NAA resulted in more excellent root regeneration. In general, IBA was more vigorous for the root formation than NAA, The treatment with 50 ppm kinetin or 100 ppm BA brought good result for the retardation of senescence of stem cuttings and BA treatment was more effective than kinetin. As for the saponin content of roots derived from stem cutting culture, the roots, formed by non-treatment of growth regulators, were higher in saponin content than those formed by treatment of growth regulators.

• Journal of Ginseng Research
• /
• 제5권1호
• /
• pp.35-40
• /
• 1981

Explants of mature root tissues and calli derived from root and cotyledon of Panax ginseng were cultured in vitro on Murashige and Skoog medium supplemented with 2, 4-dichlorophen-oxyacetic acid(3,4-D), naphthaleneacetic acid(NAA), benzyladenine, and gibberellic acid to assess their capacity to regenerate organs. Root formation at high percentage (46.2-61.1%) was obtained 20-30 days after culturing on media supplemented with combinations of NAA(5 mg/l) and kinetin (1 mg/l), And calli derived from cotyledon produced numerous embryoids in media($\frac{1}{2}$MS) containing 2,4-D(0.5 mg/l) and kinetin (0.5 mg/l). Reculture of these embryoids in media($\frac{1}{2}$MS) enriched with 1 mg/l of benzyladenine and 1 mg/l of gibberellic acid resulted in more plantlet regeneration.

• Journal of Plant Biology
• /
• 제38권4호
• /
• pp.337-341
• /
• 1995

Hairy roots of Korean ballon flower (Platycodon grandiflorum A. DC) were induced from the root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene [lobetyol (1), lobetyolin (2) and lobetyolinin (3)] production fo ten hairy root clones cultured in 1/4 Gamborg B5 (B5) liquid medium were determined. One selected hairy root clone (D6) grew well in hormone free-B5 liquid medium and showed maximum content of polyacetylenes at week 6 for 1 (0.375% dry wt) and at week 7 for 2 and 3 (3.030% and 0.206% dry wt, respectively) whose levels were much higher than those of the intact plant root (1:0.019%, 2:0.077% dry wt, 3 was not detected).

• Journal of Ginseng Research
• /
• 제20권3호
• /
• pp.256-261
• /
• 1996

Comparative biological activities of 70fr methanol extracts from the main roots and rhizomes of both red and white ginsengs were investigated using several in vitro experimental models. The main root of red ginseng and the rhizome of white ginseng strongly inhibited lipld peroxidation of hepatic microsomes induced by the non-enzymatic $Fe^{+}$ / Ascorbate system. The main root and rhizome of red ginseng markedly inhibited the release of G07, GPT and LDH by $CCl_4$-induced cytotoxicity in primary cultured rat hepatocytes as compared with those of white ginseng. And also, the main root of red ginseng showed a slight differentiating activity on HL-60 cancer cell line. The results suggest that the rhizome of ginseng have potential as a source of medicinal crude drug with possible pharmacolobica1 applications .

• 한국균학회지
• /
• 제50권1호
• /
• pp.41-46
• /
• 2022

Gastrodia elata infected with root rot disease was collected from cultivated G. elata fields in Gimcheon, Korea, in 2018. G. elata tuber surfaces exhibited root rot disease symptoms of dark-grey lesions and white fungal mycelial growth. The fungus was isolated from symptomatic tubers and cultured. Based on morphological characteristics and molecular analysis of the internal transcribed spacer region of ribosomal DNA and translation elongation factor 1-alpha, the isolated fungus was Fusarium oxysporum. This is the first report of root rot caused by F. oxysporum on G. elata tubers in Korea.

• Rapid Communication in Photoscience
• /
• 제5권1호
• /
• pp.11-12
• /
• 2016

Schwann cells and neuronal cells from dorsal root ganglion (DRG) in embryos of rat were cultured in vitro respectively. The purified neuronal cells with anti-mitotic agents and purified Schwann cells were co-cultured and then accomplished myelination processing. Infection of Semliki forest virus into this myelinated co-culture system was performed and then accomplished demyelination. We identified myelination and demyelination processing using antibody of neuropeptide Y.

• 대한본초학회지
• /
• 제24권3호
• /
• pp.13-19
• /
• 2009

Objectives : This study was aimed to define the protective effect of Tissue Cultured Root of Wild Ginseng (CWG) against doxorubicin (Doxo) toxicity, and investigate the anti-tumor synergic effect of CWG in combination with Doxo in tumor-bearing C57BL/6 mice. Methods : Tumor-bearing mice were established by single inoculation with B16/F10 melanoma cells (2$\times$10$^6$/ml) subcutaneously. Tumor-bearing mice (tumor volume between 50-100 mm$^3$) were selected and divided them into control, Doxo, and Doxo+CWG group. Mice of Doxo group were received with Doxo (4 mg/kg of B.W.) intraperitoneally at 0, 4, 8 days after starting the experiment. Mice of Doxo+CWG group were received CWG water extract during 12 days in combination with Doxo treatment. The body weight, tumor volume, tumor weight, and organ weight (heart, liver, kidney, and testis) were measured. And serum SPK, GOT and creatinine values were analysed. Results : The volume and weights of tumor masses in Doxo group were decreased significantly compared with the those of control group. And the those of Doxo+CWG group were not significantly different from the those of Doxo group. Whereas the weight of body, liver, kidney and testis in Doxo+CWG group were increased significantly compared with the those of Doxo group. The level of serum CPK and GOT in Doxo group were increased compared with the those of control group. But the value of Doxo+CWG group were decreased significantly compared with the values of Doxo group. Conclusions : These results suggest that CWG has protective effect against doxorubicin toxicity. And these effect is guessed that is caused in augmentation of vital energy.

• 한국약용작물학회지
• /
• 제7권4호
• /
• pp.251-256
• /
• 1999

시호 모상근 태양에선 MS와 RCM 배지를 기본배지로 하는 몇 가지 배지를 사용하여 생장과 사이코사포닌 함량을 비교하였으며, 그 중 MS와 3RCM 두 배지에서 보이는 생장과 성분 함량의 차이가 뿌리의 조직학적 차이와 연관이 있는지를 조사하였다. 모상근의 생장은 생중량은 MS배지가 건중량은 3RCM 배지가 각각 양호하였으며, MS와 3RCM의 건중량에 대한 생중량의 비가 각각 7.6%와 14%로 MS 배지가 함수량이 높았다. 사이코사포닌 함량은 MS배지에서는 극히 낮은 함량을 나타내었으나, 3RCM 배지에서는 총함량에서 국내 1년산 재배근의 약 $2.2{\sim}5.5$배의 함량을 나타내었다. 한편, 두 배지에서 배양한 시료의 근단부와 뿌리끝 1 cm 부근에서는 조직학적인 큰 차이를 관찰할 수 없었으나, 성숙대에서 는 MS 배지 보다 3RCM 배지에서 목부 사부 세포들의 발달이 보다 뚜렷하고, 3RCM 에서는 많은 세포에서 탄닌을 함유하는 것으로 추정되는 진한 염색의 액포 또는 세포 소기관이 관찰되었다. 따라서 이러한 세포 소기관의 발달은 사이코사포닌을 포함한 2차대사물질의 생합성과 연관이 았는 것으로 사료된다.