The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effect of the Water Extract of Tissue Cultured Root of Wild Ginseng Against Doxorubicin Toxicity

배양산삼 추출액의 Doxorubicin 독성 완화 효과

  • Choi, Kyung-Un (Department of Physiology, College of Oriental Medicine, Semyung University) ;
  • Lee, Pyeong-Jae (Department of Natural Medicinal Resources, Semyung University) ;
  • Kim, Ho-Hyun (Department of Physiology, College of Oriental Medicine, Semyung University)
  • 최경운 (세명대학교 한의과대학 생리학교실) ;
  • 이평재 (세명대학교 자연약재과학과) ;
  • 김호현 (세명대학교 한의과대학 생리학교실)
  • Published : 2009.09.30
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Abstract

Objectives : This study was aimed to define the protective effect of Tissue Cultured Root of Wild Ginseng (CWG) against doxorubicin (Doxo) toxicity, and investigate the anti-tumor synergic effect of CWG in combination with Doxo in tumor-bearing C57BL/6 mice. Methods : Tumor-bearing mice were established by single inoculation with B16/F10 melanoma cells (2$\times$10$^6$/ml) subcutaneously. Tumor-bearing mice (tumor volume between 50-100 mm$^3$) were selected and divided them into control, Doxo, and Doxo+CWG group. Mice of Doxo group were received with Doxo (4 mg/kg of B.W.) intraperitoneally at 0, 4, 8 days after starting the experiment. Mice of Doxo+CWG group were received CWG water extract during 12 days in combination with Doxo treatment. The body weight, tumor volume, tumor weight, and organ weight (heart, liver, kidney, and testis) were measured. And serum SPK, GOT and creatinine values were analysed. Results : The volume and weights of tumor masses in Doxo group were decreased significantly compared with the those of control group. And the those of Doxo+CWG group were not significantly different from the those of Doxo group. Whereas the weight of body, liver, kidney and testis in Doxo+CWG group were increased significantly compared with the those of Doxo group. The level of serum CPK and GOT in Doxo group were increased compared with the those of control group. But the value of Doxo+CWG group were decreased significantly compared with the values of Doxo group. Conclusions : These results suggest that CWG has protective effect against doxorubicin toxicity. And these effect is guessed that is caused in augmentation of vital energy.

Keywords

References

  1. 신순식, 김경철, 최영현, 이용태, 엄현섭, 김창식. 산삼 감정 기준의 객관성. 동의한의연. 2001 ; 5 : 107-14.
  2. 徐樹楠, 朱兵占. 中醫經典通釋 神農本草經. 河北 : 河北科學技術出版社. 1996 ; 10-1.
  3. 김성진, 신순식, 서부일, 지선영. 산삼, 장뇌삼, 인삼의 항암효과에 대한 비교연구. 대한본초학회지. 2004 ; 19(2) : 41-50.
  4. 곡경승, 이선구, 권기록. pH 및 전해질 조절 산양산삼 증류약침의 Apoptosis에 관한 실험적 연구. 대한침구학회지. 2004 ; 21(6) : 1-17.
  5. 민병일, 김호현, 권기록, 서일복. 산양산삼 추출액의 항암효과 및 Doxorubicin에 의한 고환독성 방어효과. 대한약침학회지. 2007 ; 10(1) : 85-100.
  6. 이태웅. 수종의 암세포주에 대한 조직배양 산삼 부정근의 항암효과. 충북대학교 대학원. 2006.
  7. 이근경. 항암물질인 ID-6105와 조직 배양 산삼 추출물과의 병용 투여에 의한 암세포 증식 억제 상승 효과. 충북대학교 대학원. 2006.
  8. 李時珍. 本草綱目. 서울 : 高文社. 1987 ; 405-12.
  9. 江蘇新醫學院. 中藥大辭典. 대구 : 大成出版社. 1984 ; 29-36.
  10. 王浴生. 中藥藥理與應用. 北京 : 人民衛生出版社. 1983 ; 15-28.
  11. 황석연, 박선우, 박정숙, 한건. 랫트에서 2,3,7,8-Tetrachlorodibenzo-p-dioxin(TCDD) 유발 생체 독성에 대한 조직배양 산삼부정근 사포닌의 치유효과. 약학회지. 2006 ; 50(4) : 220-7.
  12. 유영근, 정민석, 이윤희, 최종완, 김중회, 백기엽. 산삼부정근 추출물의 효능․효과에 관한 연구. 대한화장품학회지. 2004 ; 30(3) : 377-83.
  13. 신미희. 산삼의 배양 및 그 응용에 관한 연구. 대한화장품학회지. 2001 ; 27(2) : 45-56.
  14. 신창환. 배양산삼이 뇌 공간지각 학습능력에 미치는 영향에 대한 연구. 원광대학교 대학원. 2004.
  15. 홍사석. 이우주의 약리학강의. 서울 : 의학문화사. 1993 : 655-6.
  16. Labonte P, Kadhim S, Bowlin T, Mounir S. Inhibition of tumor growth with doxorubicin in a new orthotopically implanted human hepatocellular carcinoma model. Hepatology Research. 2000 ; 18 : 72-85. https://doi.org/10.1016/S1386-6346(99)00087-X
  17. 정희상, 박찬국, 이승일, 문철웅, 김만우, 장경식, 정춘해, 홍순표. Doxorubicin의 백서 심장독성에 관한 연구. 대한내과학회잡지. 1992 ; 42(2) : 209-17.
  18. Abd El-Aziz MA, Othman AI, Amer M and El-Missiry MA. Potential Protective Role of Angiotensin-converting Enzyme Inhibitors Captopril and Enalapril 9against Adriamycin- induced Acute Cardiac and Hepatic Toxicity in Rats. J Appl Toxicol. 2001 ; 21 : 469-73. https://doi.org/10.1002/jat.782
  19. Mansour MA, El-Kashef HA and Al-Shabanah OA. Effect of captopril on doxorubicin-induced nephrotoxicity in normal rats. Pharmacological Research. 1999 ; 39(3) : 233-7. https://doi.org/10.1006/phrs.1998.0432
  20. Shinoda K, Mitsumori K, Yasuhara K, Uneyama C, Onodera H, Hirose M, Uehara M. Doxorubicin induces male germ cell apoptosis in rats. Arch Toxicology. 1999 ; 73 : 274-81. https://doi.org/10.1007/s002040050617
  21. Yachi K., Yamauchi H., Kikuchi H., Screening and biological evaluation of liposomal formulations containing Adriamycin, Advanced Drug Delivery Reviews, 1997 ; 24 : 123-31. https://doi.org/10.1016/S0169-409X(96)00452-8
  22. Roh YW, Ji HJ, Chai HY, Hwang SY, Nam SY, Hong JT, Sung JH, Kim HK, Kang HG, Kim YB and Kang JK. Antitumor and cardioprotective effects of a ginseng intestinal metabolite in combination with doxorubicin in sarcoma-180 tumorbearing mice. The Korean Journal of Laboratory Animal Science. 2004 ; 20(4) : 348-56.
  23. Zhou S, Palmeira CM, Wallace KB. Doxorubicininduced persistent oxidative stress to cardiac myocytes. Toxicology letters. 2001 ; 121(3) : 151-7 https://doi.org/10.1016/S0378-4274(01)00329-0
  24. Al-Majed AA, Gado AM, Al-Shabanah OA, Mansour MA. Alpha-lipoic acid ameliorates myocardial toxicity induced by doxorubicin. Pharmacological research. 2002 ; 46(6) : 499-503. https://doi.org/10.1016/S1043661802002311
  25. Deepa PR, Varalakshmi P. Protective effect of low molecular weight heparin on oxidative injury and cellular abnormalities in adriamycin-induced cardiac and hepatic toxicity. Chemico-biological interactions. 2003 ; 146(2) : 201-10. https://doi.org/10.1016/j.cbi.2003.08.003
  26. Nagi MN, Mansour MA. Protective effects of thymoquinone against doxorubicin-induced cardiotoxicity in rats. Pharmacological research. 2000 ; 41(3) : 283-9 https://doi.org/10.1006/phrs.1999.0585
  27. Eun-sung Park, Sun-don Kim, Min-hye Lee, Heung-shik Lee, Je-kyung Sung, Yeo-sung Yoon, Protective effects of N-acetylcyteine and selenium against doxorubicin toxicity in rats. Journal of Veterinary Science. 2003 ; 4(2) : 129-36.
  28. E Pereverzeva, I Treschalin, D Bodyagin, O Maksimenko, K Langer, S Dreis, B Asmussen, J Kreuter, S Gelperina. Influence of the formation on the tolerance profile of nanoparticle- bound doxorubicin in healthy rats : Focus on cardio- and testicular toxicity International Journal of Pharmaceutics. 2007 ; 337 : 346-56. https://doi.org/10.1016/j.ijpharm.2007.01.031
  29. Saad SY, Najjar TA, Al-Rikabi AC. The preventive role of deferoxamine against acute doxorubicin-induced cardiac, renal and hepatic toxicity in rats. Pharmacological research. 2001 ; 43(3) : 211-8. https://doi.org/10.1006/phrs.2000.0769
  30. Shri N Giri, Mohammed Ali Al-Bayati, Xiaogu Du, Edward Schelegle, F Charles Mohr, Solomon B Margolin. Amelioration of doxorubicin-induced cardiac and renal toxicity by pirfenidone in rats. Cancer Chemother Pharmacol. 2004 ; 53 : 141-50. https://doi.org/10.1007/s00280-003-0703-z
  31. Yilmaz S, Atessahin A, Sahna E, Karahan I, Ozer S. Protective effect of lycopene on adriamycininduced cardiotoxicity and nephrotoxicity. Toxicology. 2006 ; 218(2/3) : 164-71. https://doi.org/10.1016/j.tox.2005.10.015
  32. Mansour MA, El-Kashef HA and Al-Shabanah OA. Effect of captopril on doxorubicin-induced nephrotoxicity in normal rats. Pharmacological Research. 1999 ; 39(3) : 233-7. https://doi.org/10.1006/phrs.1998.0432
  33. Sjoblom T, West A and Lähdetie J. Apoptotic response of spermatogenic cells to the germ cell mutagens etoposide, adriamycin and diepoxybutane. Environmental and Molecular Mutagenesis. 1998 ; 31 : 133-48. https://doi.org/10.1002/(SICI)1098-2280(1998)31:2<133::AID-EM5>3.0.CO;2-N
  34. Kang JK, Lee YJ, No KO, Jung EY, Sung JH, Kim YB, Nam SY. Ginseng intestinal metabolite-I (GIM-I) reduces doxorubicin toxicity in the mouse testis. Reproductive Toxicology. 2002 ; 16 : 291-8. https://doi.org/10.1016/S0890-6238(02)00021-7