• Restorative Dentistry and Endodontics
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• v.10 no.1
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• pp.177-181
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• 1984

This study was to confirm the influence of intracanal instrumentation whether the pulp infection could be extended to periapical tissue. Fifty three teeth (24 badly decayed and infected, 29 sound teeth) were employed for this experiment and grouped as follows; 1. The specimen taken from the tip of 12 infected pulp in which reamer was inserted to the canal up to apical 1/3 and cultured as long as 48 hours. After 24 hours culture 11 cases were positive and 1 ease was negative but the time of incubation elapsed as 48 hours a negative case turned to positive. 2. Broth immersed paper disc was placed for 1 minute on the tip of 12 infected teeth with a reamer inserted to the apical end and cultured as usual manner in the incubator. At 24 hour culture the growth was significant in 9 cases and after 48 hours total 12 cases were positive. 3. Reamer was inserted to apical 1/3 on 14 sterile pulp canals and specimens obtained from the root tip were cultured for 24 and 48 hours. The results on both group were negative. 4. Similar maner with No.3 except reamer tip was rest exactly at the apex revealed only 2 cases of positive at 48 hour culture. 5. The tip of 24 reamers which reached to apical 1/3 and apex of infected canal were cultured for 24 and 48 hours. At 24 hour culture the growth was evident. 6. The tip of 14 reamers which inserted to apical 1/3 of sterile canal showed negative at 24 hour culture. The 15 cases of the tip which reached to the apex of sterile canal were found negative except 3 positive cases at 48 hour culture.

• Journal of Fisheries and Marine Sciences Education
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• v.18 no.1
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• pp.1-10
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• 2006

A total of 480 questionnaires were sent out for this study and 414 collected, and 410 analyzed excluding inadequate ones. The major findings were as follows:First, the middle school teachers recognized their schooling culture to be neither positive nor negative in nature, rather neutral. They felt administrative work was over emphasized in schools. Second, their overall job satisfaction was slightly above neutral. Factors of organizational structure, human relations, and work-itself showed above neutral and only 'reward and self-actualization' factor below neutral: The 'reward and self-actualization' factor was hindrance to job satisfaction.Third, schooling culture and job satisfaction showed negative correlation: a toxic culture.

• Journal of Environmental Health Sciences
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• v.45 no.5
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• pp.511-519
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• 2019

Objectives: The standard method for the enumeration of environmental Legionella is culturing, which has several disadvantages, including long incubation and poor sensitivity. The purpose of this study is to demonstrate the usefulness of real-time PCR and to improve the standard method. Methods: In 200 environmental water samples, a real-time PCR and culture were conducted to detect and quantify Legionella. Using with the results of the survey, we compared the real-time PCR with the culture. Results: Each real-time PCR assay had 100% specificity and excellent sensitivity (5 GU/reaction). In the culture, 36 samples were positive and 164 samples were negative. Based on the results of the culture, real-time PCR showed a high negative predictive value of 99%, 35 samples were true positive, 105 samples were true negative, 59 samples were false positive and one sample was a false negative. Quantitative analysis of the two methods indicated a weak linear correlation ($r^2=0.29$, $r^2=0.61$, respectively). Conclusions: Although it is difficult to directly apply quantitative analysis results of real-time PCR in the enumeration of environmental Legionella, it can be used as a complementary means of culturing to rapidly screen negative samples and to improve the accuracy of diagnosis.

• Journal of the Korean Society for Railway
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• v.8 no.2
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• pp.176-181
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• 2005

The purpose of this thesis is, based on the cognitive response of employees, to depict the phenomenon and the pattern of organizational culture in A Subway system, To attain this purpose, several aspects of organizational culture are surveyed through questionaire and analyzed by SPSS. The major findings are . 1) In general, the cognitive response of employees in railway-concerned firms on culture-related aspects is awfully negative. 2) Job satisfaction and involvement in A Subway is highly related to achievement factor in terms of culture. 3) According to the analysis based on four kinds of Job Groups, certain job groups such as drivers and vehicle maintenance are seen as having exceptionally negative attitude towards every aspects of their organization. So, some careful diagnosis and approaches to the symptoms, the causes, and the treatments are urgently needed.

• The Journal of Korean Academic Society of Nursing Education
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• v.25 no.4
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• pp.424-435
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• 2019

Purpose: The purpose of this study was to investigate the relationship among nursing organizational culture, work performance, and workplace bullying of nurses, and to identify the factors that affect workplace bullying. Methods: The design of this study was descriptive research, and the data were collected from October 26 to November 5, 2018. The research participants were 210 nurses in six general hospitals in G province. The data were analyzed by descriptive statistics, a t-test, one-way ANOVA, Scheff? post-hoc test, Pearson's correlation coefficient, and a stepwise multiple regression using SPSS 22.0. Results: The workplace bullying type showed negative correlations with innovation-oriented culture, relation-oriented culture, and work performance. The workplace bullying consequence showed negative correlations with the innovation-oriented culture, relation-oriented culture, and work performance, and showed positive correlations with the workplace bullying type. The factors influencing the results of the workplace bullying consequence were workplace bullying type followed by relation-oriented culture, and unmarried status. Conclusion: Workplace bullying among nurses was related to the nursing organizational culture and work performance. Therefore, in order to reduce the turnover rate of nurses and to help them adapt well to the nursing organizational culture, a bullying prevention and intervention program is necessary.

• Tuberculosis and Respiratory Diseases
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• v.47 no.6
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• pp.747-756
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• 1999

Background: Acid-fast stain and cultures for diagnosis of pulmonary tuberculosis are primary and essential method, but have their limitation : low sensitivity and time consuming. The objective of this study is comparison of amplified Mycobacterium tuberculosis direct test(MTD) by the conventional AFB smears and cultures in the detection of Mycobacterium tuberculosis in respiratory specimens. Methods: During the period between November, 1997 and May, 1998 a total of 267 respiratory specimens (sputum 173, bronchial washing 94) from 187 patients suspected pulmonary tuberculosis were subjected to AFB smears, cultures and MID test. MID is based on nucleic acid amplification. We compared the MID with 3% Ogawa culture method. In positive AFB smear and negative MID specimen, positive culture identification between nontuberculous mycobacterium and M.tuberculosis was assesed by using Accuprobe M.tuberculosis complex probe. In negative AFB smear and negative AFB culture, MTD results are assessed by clinical follow-up. Results : 1) Compared with culture in sputum and bronchial fluid specimens, sensitivity and specificity of MTD in positive AFB smear is 79.7% and 20.0%, sensitivity and specificity of MTD in negative AFB smear specimens is 75.0% and 79.7%. 2) Discrepant analysis is assessed by clinical follow-up and other specimen results beyond study. Culture negative but MTD positive specimens were proved to be true positive and gave MTD sensitivity 79.2%, specificity of 84.4%, positive predictive value 80.5% and negative predictive value 83.2%. 3) 14 out of 31 specimens in negative AFB smear, negative AFB culture and positive MTD showed pulmonary tuberculosis diagnosed on clinical follow-up and sensitivity is 45.2%. 4) 2 out of 13 specimens in positive AFB smear, positive AFB culture and negative MID diagnosed as non tuberculous mycobacterium by Accuprobe culture. Conclusion: This study suggested that MID in respiratory specimens is simple and rapid diagnostic method, but considered adjuvant method rather than replace the conventional AFB smear and culture.

• Biotechnology and Bioprocess Engineering:BBE
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• v.4 no.1
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• pp.41-45
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• 1999

Microbial cellulose has many potential applications due to its excellent physical properties. The production of cellulose from Acetobacter xylinum in submerged culture is, however, beset with numerous problems. The most difficult one has been the appearance of negative mutants under shaking culture conditions, which is deficient of cellulose producing ability. Thus genetic instability of Acetobacter xylinum under shaking culture condition made developing a stable mutant major research interest in recent years. To find a proper type of bioreactor for the production of microbial cellulose, several production systems were developed. Using a reactor system with planar type impeller with bottoms sparging system, it was possible to produce 5 g/L microbial cellulose without generating cellulose minus mutants, which is comparable to that of static culture system.

• Proceedings of the KSR Conference
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• 2002.05a
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• pp.142-146
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• 2002

The purpose of this article is to depict the organizational effectiveness of "A" Subway Corporation based on the cognitive response of employees(subway drivers). To attain this purpose, several aspects of current personnel aid organization management system and organizational culture are surveyed through questionaire and analyzed by SPSS. The questionaire includes two parts of items. First, it includes items on personnel-related factors such as evaluation system job, wage, role, leadership and job satisfaction. Second, it includes items on organizational culture model suggested by Rousseau. The major findings are : 1) In general the cognitive response of driven concerning personnel-related aspects is to a certain degree negative. 2) The cognitive response of drivers concerning culture-related aspects is awfully negative. 3) Job satisfaction of drivers is highly related to role and leadership factors in terms of personnel-related aspects, on the one hard, and to achievement factor in terms of culture, on the other hand.

• Journal of The Korean Society of Emergency Medicine
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• v.29 no.5
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• pp.474-484
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• 2018

Objective: The time to positivity (TTP) of blood culture reflects bacterial load and has been reported to be associated with outcome in bloodstream infections. This study was performed to evaluate the relationship between the TTP of blood culture and the mortality rates associated with sepsis and septic shock according to the site of infection. Methods: We performed a retrospective cohort study on patients with sepsis and septic shock. The rates of blood culture positivity and mortality as well as the relationship between the TTP and 28-day mortality rate were compared among patients with different sites of infection, such as the lungs, abdomen, urogenital tract, and other sites. Results: A total of 2,668 patients were included, and the overall mortality rate was 21.6%. The rates of blood culture positivity and mortality were different among the different infection sites. There was no relationship between the TTP and mortality rates of total, lung, and urogenital infections. Patients with abdominal infections showed a negative correlation between the TTP and 28-day mortality rate. In patients with abdominal infections, a TTP<20 hours was independently associated with 28-day mortality compared with patients with negative blood culture (hazard ratio, 1.73; 95% confidence interval, 1.16-2.58). However, there was no difference in mortality rates of patients with a $TTP{\geq}20$ hours and a negative blood culture. Conclusion: The shorter TTP in patients with abdominal infections in sepsis and septic shock was associated with a higher 28-day mortality rate.

• The Journal of the Korea Contents Association
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• v.16 no.5
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• pp.497-508
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• 2016

The purpose of this study is to investigate the effect of organizational culture on subjective well-being in organization. For this, this study surveyed organizational culture and subjective well-being those 142 workers experienced. CVM(Competing Value Model) and COMOSWB(Concise Measure of Subjective Well-Being) were used to measure them. The results were as follows: First, organizational culture had a significant influence on the subjective well-being(p<.001) in the organization and explained it as 28.4%. Only group culture had a significant positive influence on the subjective well-being(p<.001). On the other hand, hierarchical culture, developmental culture and rational culture had no significant influence on subjective well-being. Second, organizational culture had a significant positive influence on satisfaction(p<.001) and positive emotion(p<.001), but had a negative influence on negative emotion(p<.05) which are the factors of subjective well-being, and explained them as 35.3%, 26.0% and 5.1%. Only group culture had a significant positive influence on satisfaction(p<.001) and positive emotion(p<.001), but had negative influence on negative emotion(p<.01). Based on the drawn results, this study provides practical and theoretical implications and suggests further studies.