• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.468-473
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• 2009

The antibiotic meroparamycin was produced in the free culture system of Streptomyces sp. strain MAR01. Five solid substrates (rice, wheat bran, Quaker, bread, and ground corn) were screened for their ability to support meroparamycin production in solid-state fermentation. In batch culture, wheat bran recorded the highest antibacterial activity with the lowest residual substrate values. The highest residual substrate values were recorded for both ground corn and Quaker. On the other hand, no antibacterial activity was detected for rice as a solid substrate. The use of the original strength of starch-nitrate medium in the solid-state fermentation gave a lower antibacterial activity compared with the free culture system. Doubling the strength of this medium resulted in the increase in the activity to be equivalent to the free culture. The initial pH (7.0) of the culture medium and 2 ml of spore suspension (1 ml contains $5{\times}10^{9}spores/ml$) were the optima for antibiotic production. The water was the best eluent for the extraction of the antibiotic from the solid-state culture. Ten min was enough time to extract the antibiotic using a mixer, whereas, 60 min was required when shaking was applied. Semicontinuous production of meroparamycin using a percolation method demonstrated a more or less constant antibacterial activity over 4 runs ($450-480{\mu}g/ml$). The semicontinuous production of the antibiotic was monitored in a fixed-bed bioreactor and the maximum activity was attained after the fourth run ($510{\mu}g/ml$) and the overall process continued for 85 days.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.445-450
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• 1992

An enhancement of the oxygen transfer rate in a 1$\ell$ bioreactor for mammalian cell culture by using a silicone rubber tubing as an oxygenator was investigated. When the silicone membrane was used to supply oxygen to the culture broth, the oxygen transfer coefficients ($k_{\iota}a$) measured in deionized-distilled water were markedly increased. Effect of surface aeration without the tubing aeration was very low under $1.0hr^{-1}$ of $k_{\iota}a$. The enhancing effects of agitation rates on $k_{\iota}a$ were much more effective than those of aeration rates. The increase of $k_{\iota}a$ with increasing tube length was observed as a result of the large surface area for oxygen supply. However, 2 m of the tube length was adequate for a 1$\ell$ vessel. The larger blade type of impeller was effective to enhance the kLa values because of its high mixing intensity. In culture medium supplemented with 5% serum, kLa values were reduced to approximately 40% probably due to the viscosity. We also obtained the normal cell concentration of $5{\times}10^6$ cells/m$\ell$ of HepG2 on microcarriers, which could be achieved in a typical bioreactor for animal cell culture.

• Journal of Digital Convergence
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• v.19 no.12
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• pp.157-164
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• 2021

This study was conducted to investigate students' perception of the integration of Chinese ethnic minority culture into the college EFL teaching which was carried out in the fall and spring semesters with different presentation topics. One and the same questionnaire was distributed to 61 participants, involving Han Chinese and Chinese ethnic minority students, at a university in ethnic minority area in China at the end of each semester, and SPSS 20.0 was used for t-test to analyze the data. The research results showed that Chinese undergraduates have got more significant improvements in cultural cognition, emotions, attitudes, and multi-cultural values in the spring semester than those in the fall semester. All participants benefit a lot from multi-cultural activities without significant differences between Han Chinese and ethnic minority students in both semesters. It is suggested that ethnic minority culture be integrated into the college EFL teaching, along with English culture and Chinese mainstream culture, which be administered as a practical teaching mode to develop students' intercultural competence.

• 지역과문화
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• v.7 no.3
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• pp.1-21
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• 2020

Living culture emphasizes cultural activities in daily life based on local characteristics, and aims to integrate art with daily life to communicate with others through activities of local residents. In this process, the values that individuals and society should have, are evoked and restored. In order to understand the context and processes in which the values of the living culture are expressed as local culture, we analyzed the case of 'Gosaek Nongak(community band music, dance and rituals in Korea) conservation association' in Suwon area and tried to diagnose how art and daily life are integrated and restoring community life in the actual field. For this purpose, phenomenological research was conducted through literature review participatory observation, and in-depth interviews to categorize the social impacts of living culture activities and public support due to individual, community, and local influences. We found that public support for living culture led to increased artistic development and human interaction in the community, and that individuals who are thus empowered to seek artistic experience and actively interact with other people, emerge as vital subjects and practitioners of culture and arts in their local community. Furthermore, we found that the activities of living culture facilitated the formation of local identity for members and local residents. Through living culture activities, members and local residents became more aware of being members of the community and found value and meaning in participating in daily life and cultural activities.

• Journal of the Korea Safety Management & Science
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• v.17 no.2
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• pp.79-88
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• 2015

The safety culture of an organization is the product of individual and group values, attitudes, perceptions, competencies, and patterns of behavior that determine the commitment, the style and proficiency, and an organization's health and safety management. A good safety culture includes effective, appropriate safety management systems; strong safety leadership & commitment from management; participation and involvement of the workforce; and organizational learning and continuous improvement. This paper will introduce the safety culture inspection standards and process in Korea Railway. The main purpose is to get a better understanding of safety culture and to develop measuring tool. First of all we developed the composition factor of safety culture and the question set. And we prepared the base of computerization of safety culture measurement by developing of evaluation standards and weighted value.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.5
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• pp.315-324
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• 2021

The study aims to investigate new theoretical approaches in an effort to resolve conceptual contradictions regarding the impact of organizational culture on developing culture for change, first by providing socio-structural frameworks supporting intrapreneurship, and second through encouraging professional culture behavior with management practices or organizational members acting as agents of change forming a deeper culture of values and behavior. The design of this research is quantitative. This study employs survey data of managers and section heads, which met the sampling requirements. Data analysis in this research used structural equation modeling (SEM) with the help of the AMOS program. The results show that all hypotheses are supported. The findings of this study suggest that the organizational culture transformation and dyadic social intrapreneurship based on professional culture (DSIBPC) have significant positive influence as antecedents of competitive advantage. This research also shows that DSIBPC is able to act as an important mediator that fills a gap in the relationship between organizational culture transformation toward competitive advantage, as well as being an alternative strategy for organizations in an effort to increase their competitive advantage. Referring to the results of this research, organizations need to focus and pay attention to the DSIBPC.

• Journal of the International Relations & Interdisciplinary Education
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• v.2 no.2
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• pp.59-79
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• 2022

In recent years, "Korean Wave" has entered the life of college students with the carrier of popular culture such as popular music, TV plays and novels, which is unique to South Korea, and has been widely pursued by college students. The Korean wave culture inevitably exerts an important influence on their values. Based on this, on the basis of questionnaire survey, this paper takes Chinese college students as the research object. After understanding the attitude and contact status of contemporary college students towards Korean Wave culture, this paper explores the reasons for the prevalence of Korean Wave culture in China, and then specifically analyzes the influence of Korean Wave culture on Chinese college students from both positive and negative aspects. It also puts forward countermeasures and suggestions to correctly guide college students to face the Korean Wave culture from the four aspects of society, colleges, families and individuals. I hope to guide college students to rationally cope with the Korean wave culture, establish a correct outlook on life, and better integrate into the trend of The Times.

• Archives of design research
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• v.17 no.2
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• pp.181-188
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• 2004

The values of material civilizations that dominated the last century have been replaced with invisible values such as knowledge, information, and culture. The central axis of the world economy is also rapidly shifting from a capital and labor-intensive industry to a knowledge-based industry of software and contents. From the perspective of the industrialization of culture as multinational corporations and dominance and subordination relationships between cultures thereof, and crisis of cultural identity, the issues of support and fostering a culture industry arise. These have become major issues of concern in deciding national policies. In response to this trend, new departments related to culture contents in various universities have been established for fostering manpower in the field and the government policy to foster related human resources. However, actual results have not been currently achieved, despite the increase in quantity. This is due to the fact that the walls between culture contents departments were too high and thus education and fostering excellent human resources have not been properly conducted. It is also due to the obscure direction of education and a deficiency in the system. To cope with these problems, a systematic manpower-fostering education program should be developed through enhancement of the understanding of culture contents via linking education programs with detailed major fields of study. Accordingly, this study aims to identify changes in the current culture contents industry at home and abroad according to digitalization for fostering manpower of culture contents required in a rapidly changing environment of the culture contents industry. Then, it will review what influence has been made on the education system through the identification of personnel types through work flow charts and contents delivery modes following integration, as well as a desirable method to construct culture contents. Therefore, this study is a preliminary study on the means of fostering core personnel in the culture contents industry through linkage with the education system.

• Microbiology and Biotechnology Letters
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• v.24 no.3
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• pp.380-383
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• 1996

Adenosine deaminase inhibitor was extracted from culture broth of Streptomyces sp. strain V-8 with ethylacetate. The ethylacetate extract showed the characteristic UV absorption spectrum of actinomycins at 440-450 nm. The ethylacetate extract was compared with respect to inhibitory behavior against adenosine deaminase from calf intestinal mucosa with actinomycin D, -C complex and actinomycin V. The Ki values for actnomycin D, -C complex, and actinomycin V against adenosine deaminase were determined to be 9.9 $\times$ 10$^{-6}$ M, 9.6 $\times$ 10$^{-6}$ M and 9.3 $\times$ 10$^{-6}$ M, respectively. The Ki value for the ethylacetate extract of culture broth against adenosine deaminase was determined to be 5.7 $\times$ 10$^{-6}$ M. The kinetic parameters of actinomycin D, -C complex, -V and ethylacetate extract of culture broth for adenosine deaminase were as follows:I$_{50}$ = 1.5 $\times$ 10$^{-5}$ M (actinomycin D), 2.7 $\times$ 10$^{-5}$ M (actinomycin C complex), 3.5 $\times$ 10$^{-5}$ M (actinomycin V), 8.9 $\times$ 10$^{-6}$ M (ethylacetate extract of culture broth). The adenosine deaminase was inhibited noncompetitively by ethylacetate extract of culture broth as well as by actinomycin D, -C complex and actinomycin V.

• Proceedings of the KSR Conference
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• 2009.05a
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• pp.1687-1690
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• 2009

It is important to safety culture in railway industry because they variously refer to the safety-related values, attitudes, beliefs, risk perceptions and behaviours of railway employees. So this study tried to figure out the assessment criteria for safety culture at Korean National Railway. For the achieve the goal, we have the literature review, some assessment criteria have been founded by intensively comparing safety culture questionnaires in other industries to establish domestic railway safety. This study expects Korean railways to become a well-organized that as assessing safety culture with reasonable criteria or requirements.