• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.212-212
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• 2017

This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.7-15
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• 2018

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

• Journal of Biosystems Engineering
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• v.15 no.4
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• pp.328-338
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• 1990

The objective of this study was to develop automatic systems of nutrient solution management for optimal nutrient solution environment and labor saving in water culture which enables factory crop production. In this study, an automatic control system and its driving program are developed to prepare, supply, and recover nutrient solution and to keep the optimal solution concentration level using microcomputers. Based on this study, the following conclusions are obtained: 1. The concentration measured by the system using oscillating circuit designed and built in this study, gave good agreements with the actual nutrient solution. 2. In water culture, the period of 12 hours for measuring concentration, pH, and temperature of the nutrient solution was optimum. Addition of control solution due to the decrease of the nutrient solution concentration is required in every 3 to 5 days. 3. It is estimated that the period of the whole solution change is 15 days, however, further research is needed to assure it. In addition, this period must be shortened in the future. 4. Both the hardware and software of the developed optimal nutrient solution control system in the water culture are working very well, however, it is necessary to develop a more economical one-chip micro controller to substitute for the microcomputer.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.263-268
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• 2005

The purpose of this experiment was to select proper nutrient solution for the water culture of young welsh onion (Allium fistulosum L.). Using two welsh onion varieties, 'Geumjanguedaepa' and 'Tokyokuro', which are good for water culture, four different nutrient solutions which were Chiba Agricultural Experiment Station solution for welsh onion, M's solution for leafy vegetables, Takekawa's solution far welsh onion, and Yamazaki's solution for welsh onion seedling were tested. Among the four nutrient solutions, increments of fresh weight and dry weight per block were the highest in Yamazaki's solution and, in the next, were placed by the order of M's solution, Chiba Agricultural Experiment Station solution and Takekawa's solution. Considering all the other results and fresh weight and dry weight increments, Yamazaki's solution for welsh onion seedling was selected as the best nutrient solution for water culture of young welsh onion.

• Journal of Bio-Environment Control
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• v.2 no.1
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• pp.9-15
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• 1993

Chinese white cabbage, Chinese flat cabbage, lettuce, garland chrysanthemum, and green perilla were grown in nutrient solution culture to investigate the effects of various media and nutrient solutions. The culture media were sand, mixed substrate(peatmoss : sand= 1 : 1), and non-media(deep-flow culture). The nutrient solutions were Cooper's, Hoagland's, and Yamazaki's solution. Plants were grown under different treatments for three weeks. Generally, the growth was greatest in non-media culture and followed mixed substrate culture, and poorest in sand culture. In non-media culture, the growth of Chinese white cabbage, Chinese flat cabbage, lettuce, and green perilla was good in Yamazaki's solution. And regardless of nutrient solution, garland chrysanthemum was good in non-media culture. Relative chlorophyll was not different among the treatments.

• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.35-40
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• 1999

$N(^{15}N)$ and $P(^{32}P)$ absorption by 2 year-old Cymbidium Jungfrau in solution culture were investigated. Growth, photosynthesis rate, chlorophyll content and mineral composition of Cymbidium in the solution culture with bark or granular rockwool were compared with these parameters in the conventional pot culture. Nitrogen absorption by Cymbidium was higher in full sunlight than in 60% of sunlight while P absorption was higher in 60% of sunlight. Sixty seven % of N absorbed in plant was redistributed to the bulb(39%) and leaves(28%) while 46% of P absorbed was fund in the bulb (36.2%) and leaves (10.2%). Accumulation of P in leaves was 3-fold lower than that of N. N and P absorption in 0.5 or 1 year- old daughter plant Bowing vigorously were greater than in immature daughter or mother plant. The absorption rate of phosphorus in Cymbidium was 350-fold lower than that of barley. Greater shoot length and bulb diameter, and higher fresh weight, photosynthesis rate and chloroployll content were observed in the solution culture than in the conventional pot culture. Solution culture had-also more content of N, P, K and Mg in leaves, bulb and root than conventional pot culture but did not that of Ca. A large part of the nutrient absorption was occurred during vegetative growth. Also, There was no difference between bark and rockwool in the solution culture due to the improvement of poor dispersion of nutrient solution in bark.

• Magazine of the Korean Society of Agricultural Engineers
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• v.41 no.2
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• pp.37-43
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• 1999

This study was conducted to develop the simple and convenient irrigation control model which can maintain the appropriate rates of irrigation and drainage of nutrient solution according to the enviornmental conditions and growth stages in soilless culture of cucumber. In order to obtain fundamental data for development of the model, investigation of the actual state of soilless culture practices was carried out. Most irrigatioin systems of soillness culture were controlled by the time colock. Evapotranspiration of cucumber in soilness culture was investigated and correlations with environmental conditions were analyzed , and its estimating model was developed. In order to develop the irrigation system which can control the amount of nutrient solution applied according to seasons, weather conditions, and growth stages, a irrigation clock control was developed. Applicability of the model was tested by simulation. Drainage rates of nutrient solution controlled by conventional time clock, integrated solar radiation, and the developed model were 61% , 20%, and 32% , respectively in cucumber perlite culture.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2002.05a
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• pp.147-147
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• 2002

This experimental study was carried out to investigate on the safety of Nog-yong aqua-acupuncture solution in primary culture of adult rat hepatocytes. Primary culture of adult rat hepatocytes has been considered as a ideal model for toxicological studies because cultured hepatocytes maintained many liver-specific functions. In this research, we investigated the effects of Nog-yong aqua-aqupunture(1-10$\mu\textrm{g}$/ml) on cytotoxicites in primary culture of adult rat hepatocytes using LDH release assays. Hepatic glutathione level. glutathione-S-transferase activity, and albumin synthesis were not affected by treatment with Nog-yong aqua-aqupuntur alone. Nog-yong aqua-aqupuncoure solution(0.5-$10{\mu}\textrm{g}$/ml) on cytotoxicites in primary culture of adult rat hepatocytes using LDH release not significantly affected normal functional charaterists.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.554-561
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• 1999

A novel estimation method was investigated for determining the concentrations of residual biomass, poly-3-hydroxybutyrate (PHB), and main nutrients including carbon and nitrogen sources, phosphate, and mineral ions from the supplied amount of ammonia solution used for a pH-control solution and nitrogen source in a PHB fermentation. The estimation equations for a batch culture and a fed-batch culture were derived from the relationship between the growth rate of residual biomass and the feed rate of the pH-control solution, and then were applied to the batch culture and the fed-batch cultures of Alcaligenes latus. This method was successfully applied to estimate the concentrations of residual biomass, PHB, and nutrients.

• Review of Korea Contents Association
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• v.14 no.2
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• pp.59-63
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• 2016