Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Estimation of Residual Biomass, PHB, and Nutrient Concentrations by Supplied Amount of Ammonia Solution in Fermentation of Alcaligenes latus

  • Lee, Yong-Woo (Research Institute of Biodegradable Polymers, Department of Biochemical Engineering, College of Engineering, Yanbian University of Science and Technology) ;
  • Tsuneo Yamane (Department of Applied Biological Sciences, School of Bioagricultural Sciences, Nagoya University)
  • Published : 1999.10.01
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Abstract

A novel estimation method was investigated for determining the concentrations of residual biomass, poly-3-hydroxybutyrate (PHB), and main nutrients including carbon and nitrogen sources, phosphate, and mineral ions from the supplied amount of ammonia solution used for a pH-control solution and nitrogen source in a PHB fermentation. The estimation equations for a batch culture and a fed-batch culture were derived from the relationship between the growth rate of residual biomass and the feed rate of the pH-control solution, and then were applied to the batch culture and the fed-batch cultures of Alcaligenes latus. This method was successfully applied to estimate the concentrations of residual biomass, PHB, and nutrients.

Keywords

References

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