• Title/Summary/Keyword: Culture Technology

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Analysis of Microbial Community During the Anaerobic Dechlorination of Tetrachloroethylene (PCE) in Stream of Gimpo and Inchon Areas (경기도 김포, 인천 서구지역 소하천의 PCE 탈염소화 군집의 선별 및 다양성 분석)

  • Kim, Byung-Hyuk;Baek, Kyung-Hwa;Cho, Dea-Hyun;Sung, Youl-Boong;Ahn, Chi-Yong;Oh, Hee-Mock;Koh, Sung-Cheol;Kim, Hee-Sik
    • Korean Journal of Microbiology
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    • v.45 no.2
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    • pp.140-147
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    • 2009
  • In this study, anaerobic enrichment cultivation was performed with the sediments from the Gimpo and Inchon areas. Lactate as an electron donor and PCE as an electron acceptor was injected into the serum bottle with an anaerobic medium. After the incubation of 8 weeks, the reductive dechlorination of PCE was observed in 7 sites among 16 sites (43%). Three enrichment cultures showed completely dechlorination of PCE to ethene, while four enrichment culture showed transformation of PCE to cis-DCE. The bacterial community structure was analyzed by PCR-DGGE. Dechlorinating bacteria were detected by species-specific primers. The dominant species in seven anaerobic enrichments were found to belong to the genus of Dehalococcoides sp. and Geobacter sp., and Dehalobacter sp.

The Landscape Characteristics of Utopia Shown in the Travel Records of Jirisan Mountain (지리산 유람록에 나타난 이상향의 경관 특성)

  • So, Hyun-Su;Lim, Eui-Je
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.32 no.3
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    • pp.139-153
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    • 2014
  • This study contemplates the utopian landscape recognized by the scholars with twenty three pieces of 'travel record'. Consequently, five key words - Mureungdowon(武陵桃源), Byeolcheonji(別天地), Dongcheon(洞天), Chonghakdong, Eungeoji(hermitage) - are chosen for comprehending the utopia and their landscape characteristics are organised as follows. Mureungdowon in Jirisan Mountain which the scholars dreamed of is a flatland with the full energy for local vegetation and domestic animals in the mysterious and deep gorge. This utopia eventually reflects the rural landscape. Byeolcheonji is a utopia combining the concept of a fairyland and beautiful scenery. The scholars also used the term 'Dongcheon' for naming the enclosed landform which is suitable for seclusion and defining the some areas of beautiful scenery. Cheonghakdong, which is set only in Jirisan Mountain, has been formed by the stone scenery of gorges and Buril waterfalls around the whole area of Burilam Hermitage, the vegetation scenery of pine trees and bamboos with the legend of Choi Chiwon and his engraved inscription on a rock. Adding to the utopia passed down, the scholars perceived the village with geographical features with back to the mountain and facing the water, the river practising the trade, the flatland enclosed by bamboo forests, the vegetation mainly consisting of fruit trees and beautiful scenery as the utopia realized on earth. It is equivalent to the world of human beings laboring appropriately and living in Mother Nature. As mentioned above, this study has significance for apprehending the relevance between the culture of strolling in the mountains by the scholars of Joseon Dynasty and the fairyland and explaining the various traditional utopias from the inherited concepts from China to the naturalized realistic utopia.

Organic Matter and Hydraulic Loading Effects on Nitrification Performance in Fixed Film Biofilters with Different Filter Media

  • Peng, Lei;Oh, Sung-Yong;Jo, Jae-Yoon
    • Ocean and Polar Research
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    • v.25 no.3
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    • pp.277-286
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    • 2003
  • Nitrification performance of fixed film biofilters using coarse sand, loess bead, or styrofoam beads in biofilter columns 1 meter high and 30cm in diameter were studied at different hydraulic and organic matter loading rates. Synthetic wastewater was supplied to the culture tank in order to maintain desired TAN concentrations in inlet water to biofilters. All the biofilters were conditioned 5 months before start of sampling. TAN and $NO_2-N$ conversion rates increased with an increase in the hydraulic loading rate (HLR). However, the improvement in biofilter performance was not linearly correlated to HLR in styrofoam bead filters. This is mainly due to the characteristics of the styrofoam beads used. TAN conversion rates of sand filters increased with the increase of HLR up to $200m^3/m^2$. per day. No increase in the TAN conversion rate was observed at the highest HLR since flooding on the media surface took place. HLR had a significant impact on the TAN conversion rates in loess bead filter up to the highest HLR tested (P<0.05). TAN conversion rates were much less at organic matter loading rates of 9 and 18kg $O_2/m^3$ per day than those without the addition of organic matter in styrofoam bead filters. The addition of glucose resulted in a reduction of the TAN conversion rate from 540 to 284g $TAN/m^3$ per day. No significant difference of TAN conversion rates between the two organic matter loading rates was found (p<0.05). This indicates that the impact of organic matter on nitrification becomes less and less sensitive with an increase in the COD/TAN ratio. At an organic matter loading rate of 9kg $O_2/m^3$. per day, a great reduction of TAN conversion rates was observed in sand filters and loess bead filters. Clearly, organic matter can be one of the most Important Impacting factors on nitrification. $NO_2-N$ conversion rates showed a similar trend for TAN. Based on the TAN and nitrite conversion rates, styrofoam beads showed the best performance among the three filter media tested. Also, the low gravity and price of styrofoam beads make the handling easier and more cost-effective for commercial application. The results obtained at the highest organic matter loading rates can be used in the biofilter design in recirculating aquaculture system.

Optimum Stocking Density of Juvenile Abalone (Haliotis discus hannai) Fed the formulated Diet or Macroalgae (Undaria) (실험 배합사료 및 미역 공급시 참전복 치패의 적정 사육밀도)

  • KIM Byong-Hak;LEE Sang-Min;GO Chang-Soon;KIM Jae-Woo;MYEONG Jeong-In
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.6
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    • pp.869-874
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    • 1998
  • A feeding trial was carried out to investigate the optimum density of juvenile abalone (Haliotis discus hannai) fed the formulated diet or macroalgae ( Undaria). Abalone with initial average shell length of 8.41 mm were stocked into three replicate tanks at four different densities of 1430, 2860, 4290, and 5720 individual/$m^2$ for 15 weeks. The formulated diet produced higher body weight and shell growth, soft body weight and survival rate than Undaria in abalone. Abalone fed the formulated diet have significantly (P<0,05) higher lipid and ash, and lower moisture and protein in soft body. Higher density resulted in lower growth and survival (P<0.05). Whereas shell growth, survival rate and weight gain were not significantly (P>0.05) different within the abalone stocked at stocking densities of 1430 and 2860 individual/$m^2$ in each diet group. Body composition of abalone was not affected by increasing density. These study indicate that the number of abalone (shell length ranges of between 8 mm and 17 mm) below 2860 individual/$m^2$ were predicted to effective density in the indoor tank culture system.

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Microbial Communities of the Microbial Fuel Cell Using Swine Wastewater in the Enrichment Step with the Lapse of Time (가축분뇨를 이용한 미생물연료전지의 농화배양 단계에서 미생물 군집 변화)

  • Jang, Jae Kyung;Hong, Sun Hwa;Ryou, Youg Sun;Lee, Eun Young;Chang, In Seop;Kang, Young Koo;Kim, Jong Goo
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.12
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    • pp.973-977
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    • 2013
  • These studies were attempted to investigate the change of microbial community of anode of microbial fuel cell using swine wastewater in the enrichment step with the lapse of time. Microbial fuel cells enriched by a 1 : 1 mixture of anaerobic digestive juices of the sewage treatment plant and livestock wastewater. Enrichment culture step was divided into three stages to indentify the microorganisms. It was separated by each lag phase, exponential phase, and stationary phase. These steps were determined by the change of the current value. The current after enrichment was generated about $0.84{\pm}0.06mA$. We were cut out the different 17 bands in the DGGE fingerprint gel to do sequencing. The bands which the concentration was increasing or newly appearing with the lapse of time were included for this study. In the lag and exponential phase, Clostridium, Rhodocyclaceae, Bacteriodetes, and Uncultured bacterium etc. were detected. There were in the stationary phase Geobacter sp., Rhodocyclaceae, Candidatus, Nitrospira, Flavobactriaceae and uncultured bacterium etc. Geobactor among microorganisms detected in this study is known as the Electrochemically active microorganisms. It may include electrochemically active microorganisms to be considered as electrical activity microorganisms.

The Analysis of Chemistry's Image in Daily newspaper on '2006, the Year of Chemstry' (2006년 '화학의 해'의 언론매체 속 화학 이미지 분석)

  • Shin, Sunyoung;Cho, Mijoo;Park, Jongseok
    • Journal of Science Education
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    • v.32 no.1
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    • pp.47-60
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    • 2008
  • The Ministry of Science and Technology has been planning various events and carrying them out as the effort for promotion and popularization of science. The ministry has made followings: i)Science culture year, 2004 ii) Physics year, 2006 iii)Chemistry year, 2007 iv)Biology year, 2008. Especially, in 2006 the ministry held various exhibitions and contests about chemistry. The science play "Oxygen" had been presented in the whole country, 2006. From 2006 to 2007 the moving science museum had been displayed many times in the whole country. The national broadcast media KBS made a special program and televised several times to commemorate the year of chemistry. Like that in 2006 the chemistry approached to general people. In these surroundings, we wondered how chemistry was expressed in daily newspaper and how the general public accepted chemistry in the year of chemistry, 2006. So we selected four daily newspaper and researched descriptions about chemistry then analyzed the image of chemistry in that descriptions. When we research, we first selected 5 words of high frequency. On the internet research engine we used these 5 words and searched descriptions. We read and sorted descriptions which were more connected to chemistry. Finally we analyzed the images of descriptions(affirmative or negative) and character. The chemistry's image on newspaper descriptions influences people greatly. Because that is the easiest channel.

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Ways to Educational Improvement of Korean Oriental Medicine (한국 한의학교육의 발전방안)

  • Lee Sun-Dong;Han Yong-Joo;Shin Kyu-Won
    • Journal of Society of Preventive Korean Medicine
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    • v.8 no.2
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    • pp.99-113
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    • 2004
  • Problems and current situation of public health globally and domestically were analyzed in this study and based on these findings, ways to improve from western medicine and Oriental medicine can be deduced as follows: 1. Current problems of public health in Korea and the world 1) Increase of diseases resulted from daily habits and infectious diseases, many are at the brink of being ill. Quality of life from extended life span and unbalanced health care must be solved. 2) Natural and societal factors including host factors, public health service, and other external and internal factors play an important role in deciding healthy and being ill. 3) Some of the limits and problems of modem medicine include insufficient academic knowledge and incomplete theory, as well as misled approach to the treatment. Human itself isn't perfect organism and other realistic problems hinder one's well-being. 4) Regardless of western medicine or Oriental medicine, patients were approached as someone with diseases and disorders, and wholistic approach was disregarded. Lack of clinical training, absence of clear educational philosophy and goal are some of the reasons why the education isn't under concrete system 2. Important factors for the medical education and proper direction for the education of Oriental medicine 1) Important factors for medical education - Education should not be limited on the human health and illness, but also cover qualities such as well-being, social welfare, service, and happiness. Every aspects of human life must be considered and attended for more productive outcome. - Basic understanding of humanity must be included in the educational curriculum - Foundation of human diseases and pain are associated with inner life and surrounding causes including family, society, nature, race, culture, religion, politics, and etc., thus the education must be approached to recognize aforementioned criteria. 2) Proper direction for the education of Oriental medicine - Values of Oriental medicine for medical principles and importance of lifehood must be educated. - Educational goal, limits, and levels must be established for the school of Oriental medicine - Respect for life must be the top priorities of educational direction which should lead to solution based education for the human health. Latest medical theory and technology should be accommodated as well as prevention, treatment, and balancing of basic courses and clinical training for optimal education.

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Investigation of Transformation Efficiency of Rice Using Agrobacterium tumefaciens and High Transformation of GPAT (glycerol-3-phosphate acyltransferase) Gene Relative to Chilling Tolerance (Agrobacterium tumefaciens를 이용한 벼의 형질전환 효율의 검토 및 내한성 관련 GPAT (glycerol-3-phosphate acyltransferase) 유전자의 형질전환)

  • Seo, Mi-Suk;Bae, Chang-Hyu;Choi, Dae-Ock;Rhim, Seong-Lyul;Seo, Suk-Chul;Song, Pill-Soon;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.85-92
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    • 2002
  • This study has been focused on improving transformation efficiency of rice using Agrobacterium tumefaciens. We have demonstrated the effect of this system when the GPAT gene related to the cold-resistance was transferred by Agrobacterium tumefaciens in rice. Transformation conditions were modified using intron $\beta$-glucuronidase (GUS) expression as a reporter gene in the rice. In this study, mature seed-derived calli of rice (Oruza sativa L. cv. Dongjin) were pre-cultured for 3 days and then infected with Agrobacterium. When this infected calli were cultured in the dark for 10 days on co-cu]lure medium containing 50 mg/L of CaCl$_2$, 30 mg/L of acetosyringone, 2 mg/L of 2,4-D, 120 mg/L of betaine, high GUS expression was observed. In the present transformation system, the efficiency of transformation of GPAT gene was about 54%. Stable integration of GPAT gene into chromosomal DNA was proven by southern blot analysis of genomic DNA isolated from T$_{0}$ progenies. The progenies (T1 generation) derived from primary transformant of 5 lines were segregated with a 3 (resistant) : 1 (sensitive ratio) in medium containing hygromycin. This high frequency transformation system can be used as a useful tool in transformation of another monocotyledon.n.

Endoreduplication Pattern of Somatic Embryos and Variants Occurrence Affected by Pre-existed Endoreduplicated Cells in Doritaenopsis (Doritaenopsis 체세포배의 내배수성 특성과 절편체의 내배수성 세포에 기인한 체세포변이의 발생)

  • Park, So-Young;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.297-302
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    • 2006
  • In general, the proliferation of orchids via somatic embryos has been used for mass production of somatic clones because of high propagation efficiency. In spite of high propagation rate, this method often brings somaclonal variation, especially polyploid frequency. Therefor we here concentrated to investigate the relationship between endopolyploidization patterns of explants and the occurrence of tetraploid variant in clonally proliferated Doritaenopsis via somatic embryo regeneration system. In the fully developed somatic embryo, upper part contained 2C to 16C while middle and lower parts showed 2C to 32C DNA content. Two-week-old embryo contained 2C to 16C, whereas those regenerated after 4 to 10-week-old contained 2C to 64C nuclei. Results showed that endoreduplication was variable depending upon tissue types, ages, and parts in one species. lower part of somatic embryo having high endoreduplication degree increased the regeneration of tetraploid variants by about 3-fold comparing to upper part of somatic embryo culture. polyploid frequency occurrence might be closely related to the high levels of endoreduplication of somatic embryos used as explant. It suggested that the upper part of somatic embryo having comparatively low endoreduplication degree is suitable for the stable in vitro propagation system.

A basic study on development of Women's Fashion Design using Global Market Oriented-Supersensitive Jacquard (글로벌 마켓 지향 고감성 자카드를 활용한 여성복 디자인 개발에 대한 기초적 연구)

  • Kim, Young-Mi;Cho, So-Young;Ahn, Hee-Jung
    • Journal of Fashion Business
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    • v.14 no.4
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    • pp.91-101
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    • 2010
  • This paper has the purpose of suggesting a development-method on the fashion products that would secure the competitiveness in the global markets through creating supersensitive practical products on the basis of developing originative fashion-goods being made of jacquard that is fine quality of couture. This paper has collected data through the cases of the developed nations in fashion, precedent studies and all the related literature on the subject. The results of this researching are as following: The first, it is expected that the necessity of developing new fashion products would be appealed toward the prestige group of consumers who are seeking for the fine quality and super-sensitivity in female fashion in Korea, and it is the current situation that the products made of jacquard are gradually expanding not only in the area of apparel, fashion goods and interior but also another areas. The current situation of the global market suggests the necessary strategy of survival, that is, the development of supersensitive materials and creative products which would be able to keep the high quality and lower the selling price through cost reduction. The second, the suggestion of the direction in developing the products of the female fashion made of jacquard has two points - the development of the texture that would realize a unique form and the development of the material that would be able to realize planar pattern and three dimensional pattern which are woven with thin and light materials with various solidity and delicacy through various techniques of mixing and three dimensional expression. The third, the expected ripple effect and utilization generated from the development of fashion products are as followings: As material characteristics of jacquard, It needs the specialization of various techniques and specialized production system in using jacquard, and the specialized technique and system would make it possible to produce not only the higher value-added products through expressing affluent colors and delicate design but also competitive products through cutting the process and cost, eventually, it would lead to the expansion of the jacquard market of super-sensitive prestige. Therefore, it is remarkable that various development of products toward the global market and the prestige female fashion market can suggest the vision that make the national fashion industry develop into the higher value-added knowledge industry integrating technology and culture.