• Title/Summary/Keyword: Culture Fusion

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Mycotic Abdominal Pseudoaneurysm due to Psoas Abscess after Spinal Fusion

  • Ryu, Dae Woong;Lee, Sam Youn;Lee, Mi Kyung
    • Journal of Chest Surgery
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    • v.48 no.6
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    • pp.443-446
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    • 2015
  • A 36-year-old man, who had undergone thoracoscopic anterior spinal fusion using the plate system and posterior screw fusion three months previously, presented to our hospital with left flank pain and fever. Computed tomography indicated the presence of a psoas muscle abscess. However, after two days of percutaneous catheter drainage, a mycotic abdominal aortic pseudoaneurysm was detected via computed tomography. We performed in situ revascularization using a prosthetic graft with omental wrapping. Methicillin-resistant Staphylococcus aureus was identified on blood and pus culture, and systemic vancomycin was administered for one month. Although the abscess recurred, it was successfully treated with percutaneous catheter drainage and systemic vancomycin administration for three months, without the need for instrumentation removal. The patient remained asymptomatic throughout two years of follow-up.

Intergeneric protoplast fusion between Gliocladium virens and Trichoderma harzianum (Gliocladium virens 와 Trichoderma harzianum의 속간(屬間) 원형질체융합(原形質體融合))

  • Shin, Pyung-Gyun;Cho, Moo-Je
    • The Korean Journal of Mycology
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    • v.21 no.4
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    • pp.323-331
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    • 1993
  • The protoplast formation and intergeneric protoplast fusion between Gliocladium virens and Trichoderma harzianum were attempted to obtain fusants. Protoplast formation was the most effective when the strains were treated with concentration of 5 mg/ml of Novozyme 234 and Cellulase at $25^{\circ}C$ for 3 hours in phosphate buffer, pH 6.5, supplemented with 0.6 M sorbitol as osmotic stabilizer. Auxotrophic mutants of G. virens G88 did not grow in minimal medium and benomyl resistant T. harzianum T95 from wild types, however, was selected by treatment with UV light as genetic marker to isolate fusants. When the intergeneric protoplast fusion between G. virens G88 and T. harzianum T95 was carried out using 30% PEG 4000 containing 10 mM $CaCl_{2}$, and 50 mM glycine (pH 8.5) as fusogenic agent at $25^{\circ}C$ for 10-15 min, the fusion frequency was $0.8{\times}10^{-4}$. Fusants obtained from intergeneric protoplast fusion were spontaneously segregated into va rious strains by continous culture on complete medium. Several intergeneric hybrids were classified into three types: parent-like hybrids, segregants, and recombinants.

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Fusion research method for the Korean traditional Folksong ('융합' 중심의 민요연구방법론 모색)

  • Kwon, Oh-Kyung
    • (The) Research of the performance art and culture
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    • no.32
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    • pp.525-547
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    • 2016
  • The purpose of this study starts searching a new method for preservation, succession, and research of folksongs in the situation that the function and transmission of folksongs are transformed or disappear by being behind modernization. Also, trying to develop a new method of study of folksongs along with a new view of them, I put fusion in the heart of the study. Fusion means a structural change in the middle of the process of combination from more than two things. Moreover, in order to practice the fusion method of study consilience in a view of study is necessary. The interdisciplinary study beings in accepting differences between academics as long as the differences have a meaningful value disassemble and differentiation are repeated. That is, fusion means rebirth through disassemble. According to this logic, we need to disassemble the folksongs for fusion study of folksongs, research the structure and component of folksongs in a new view and understand each part of the wholeness of folksongs. On top of that, we need to try combine the parts with anything else or interpret them with consilience. In this study, I researched the previous example in the fusion or integration of folksongs and Korean classic poetry in the time of enlightenment of Korea. In addition, I studied the way of fusion folksongs as culturology, bio-sociology, and sympathy. Looking back folksongs in a variety of view, we can realize folksongs are disassembled or differentiated at the same time they reborn and are interpreted in a new view.

Designing Performance Costumes of Daily Dance Ganggangsullae for Elementary Students (초등학생을 위한 강강술래 국민생활댄스 공연복식 제안)

  • Cho, Du Na
    • Human Ecology Research
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    • v.53 no.1
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    • pp.81-92
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    • 2015
  • Daily dance Ganggangsullae is recommended for youth to help their diet, improve their team life and know Korean culture. Its performance costumes were fusion hanbok and casual wear shown at the Second Daily Dance Ganggangsullae Contest Conference of Myeongryang Battle Festival in 2013. A fusion hanbok shows the Korean traditional spirit, but it is not practical. Casual wear is practical, but it does not show any Korean identity. Therefore, their combination is needed to reflect the Korean identity and practicality. For this, a literature review and an online search were conducted for the history, purpose and dance motion of daily dance Ganggangsullae. And photographs of participating teams were gathered from Jeonnam Information & Culture Industry Promotion Agency. Goguryeo costumes were used for design motifs. Four designs were sets of T-shirts and pants for the summer. Style 1 is designed using sam, jikryeong, go, round and triangle pattern with rippled cotton. Colors were from Wang Huiji's mural painting. Style 2 is designed using dansuui, beonryeong, go, round and quadrangle patterns with rippled cotton. Colors were from a Deokheungri mural painting. Style 3 is designed using sam, round ryeong, go, quadrangle patterns, prints with cotton jersey and denim. Colors were from a Gamsinchong mural painting. Style 4 is designed using dansuui, jikryeong, go, a flame pattern, a bow and arrow shape with cotton, knit and jersey. Colors were from a Muyongchong mural painting. This study is helpful to anyone who wants to develop Korean-style performance costumes.

Design of Low Cost Real-Time Audience Adaptive Digital Signage using Haar Cascade Facial Measures

  • Lee, Dongwoo;Kim, Daehyun;Lee, Junghoon;Lee, Seungyoun;Hwang, Hyunsuk;Mariappan, Vinayagam;Lee, Minwoo;Cha, Jaesang
    • International Journal of Advanced Culture Technology
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    • v.5 no.1
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    • pp.51-57
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    • 2017
  • Digital signage is becoming part of daily life across a wide range of visual advertisements segments market used in stations, hotels, retail stores, hotels, etc. The current digital signage system used in market is generally works on limited user interactivity with static contents. In this paper, a new approach is proposed using computer vision based dynamic audience adaptive cost-effective digital signage system. The proposed design uses the Camera attached Raspberry Pi Open source platform to employ the real-time audience interaction using computer vision algorithms to extract facial features of the audience. The real-time facial features are extracted using Haar Cascade algorithm which are used for audience gender specific rendering of dynamic digital signage content. The audience facial characterization using Haar Cascade is evaluated on the FERET database with 95% accuracy for gender classification. The proposed system, developed and evaluated with male and female audiences in real-life environments camera embedded raspberry pi with good level of accuracy.

Alterations in the Level of Calpain During the Differentiation of Chick Embryonic Muscle Cells in Culture (배양 근원세포의 분화에 따른 Calpain의 활성 및 양적 변화)

  • 곽규봉;정성수;박혜경;김혜선;정진하;하두봉
    • The Korean Journal of Zoology
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    • v.33 no.2
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    • pp.158-165
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    • 1990
  • Proteolytic activity of calpain was found to increase as myoblast fusion proceeds. At 60 hr after cell seeding, lis activity reached to a maximal level and then slighdy decreased thereafter. Similarly, the protein level of calpain reached to a maximal level just proir to the initiation of fusion and remained elevated upon prolonged culture as analyzed by immunoblol using anti-calpain antiserum. These results suggest that the synthesis of calpain is regulated during myogenesis and its proteolytic activity may be related with the process of myoblasts fusion.

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Optimization of recombinant E. coli fermentation through biological manipulation and engineering control

  • Kim, Jeong-Yoon
    • The Microorganisms and Industry
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    • v.19 no.4
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    • pp.14-26
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    • 1993
  • Optimizing protein production in recombinant E. coli strains involves manipulation of genetic and environmental factors. In designing a production system, attention must be paid to gene expression efficiency, culture conditions and bioreactor configuration. Although not much emphasis was given to the physiology of host strains in this review, an understanding of the relationship between the physiology of host cell growth and the overproduction of a cloned gene protein is of primary importance to the improvement of the recombinant fermentation processes. Sometimes it is desirable to make use of gene fusion systems, e.g. protein A, polypeptide, gutathione-S-transferase, or pneumococcal murein hydrolase fusion, to facilitate protein purification.

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A Study on Intertextuality with Other Domains in the 21C Contemporary Fashion - Focused on Animation, Music, Performing Arts and Technology - (21세기 현대 패션에 나타난 타영역과의 상호텍스트성에 관한 연구 - 애니메이션, 음악, 무대예술, 테크놀로지를 중심으로 -)

  • 김혜정
    • Journal of the Korean Society of Costume
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    • v.54 no.2
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    • pp.105-119
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    • 2004
  • Cultural hybridization and global cross-fertilization, begun since the 1990s, mean the hybrid mixture of the low-class popular arts and the high-class fine arts and have found expression in intertextuality by means of the thinking system of de-constructuralist post-modernism. This study was intended to investigate the artistic intertextuality between fashion and art frontiere, especially between such fields as 'animation', 'music', and 'performing arts' and 'fashion', which shows the greatest characteristic of the phenomenon that the non-mainstream culture flows into the mainstream culture. It also aimed to investigate the intertextuality between 'technology' and 'fashion' as the delivery room of the youth culture and copycat culture due to the benefit of technologies since the 20th-century digital revolution. Animation as the neo-pop art with the popular code coming to the forefront in the 21th century integrated high-class fashion and low-class fashion into one through the combination of fashion and humor, and the musical element referring to the social difference of the cultural field and the social strata is becoming the 21th-century fashion icon through its fusion with the pictorial tendency along with the leisure-time life of the non-mainstream strata. In terms of intertextuality in performing arts, fashion style performs an important role in the presentation of performing arts and since the de-construction of the fin-de-ciecle form and its fusion with media have taken place, fashion works become and element of the origin that has an influence on a series of film, dance, dramatic elements and the like. The paradigm of technology made it socially and culturally possible to achieve the architecture of clothing system necessary for fashionable technical clothing by allowing the possibility of imitation, the function of technology to form popular culture. The intertextual tendency in the 21th-century fashion began from the de-constructive phenomenon of existing norms and now takes the multicultural character of surpassing a certain domain or concept. And it positions itself as the total art of spearheading the low-class culture and the non-mainstream culture as the hybrid domain of mainstream and non-mainstream cultures or high-class and low-class cultures.

Research of the Foreign Dancing Costumes - From Han to Sui Tang Dynasty - (중국(中國) 호무복식(胡舞服飾)에 관한 연구 - 한대(漢代)부터 수당대(隋唐代)를 중심으로 -)

  • Yoo, Ji-Won
    • Journal of the Korean Society of Costume
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    • v.56 no.3 s.102
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    • pp.57-72
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    • 2006
  • Culture speaks for the characters of the period, so it is presented by the mutual actions of many factors affecting culture. Foreign culture, introduced by cultural exchange, was modified and accepted into a new form and value system. In the beginning, only foreigners danced these dances, but Chinese started to dance them gradually. Thus, the dancing costume showed the complete fusion of Chinese and exotic styles. Especially, in the Tang dynasty, Chinese accepted foreign culture very actively and with open hearts. They accepted foreign culture based on their traditional culture, and fused them completely. In these costumes, not one culture was prominent, but many cultures from Gandhara to East and West Turkistan and even to Persian and Hellenism were synthesized together. Chinese, in the Tang dynasty, retained on their traditional culture and modified new foreign culture into Chinese style.

Purification and Characterization of $\beta$-Xylosidase from Trichoderma sp. SY

  • Kim, Bong-Gyu;Jung, Bo-Ra;Jung, Jae-Gyu;Hur, Hor-Gil;Ahn, Joong-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.643-645
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    • 2004
  • A $\beta$-xylosidase was purified from the culture of Trichoderma sp. SY. The ten-day-old culture filtrate was concentrated, followed by ion-exchange chromatography and gel filtration chromatography. As a result, $\beta$-xylosidase was finally purified about 53-fold and appeared as a single band by SDS-PAGE. The optimum pH and temperature were 5.0 and $55^{\circ}C$, respectively, and the molecular weight about 80 kDa. The purified $\beta$-xylosidase was found to be inhibited by various metal ions but not inhibited by xylose.